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#28646718   2017/06/24 Save this To Up

Reduced graphene oxide-functionalized FeOOH for signal-on photoelectrochemical sensing of prostate-specific antigen with bioresponsive controlled release system.

A new and signal-on photoelectrochemical (PEC) sensing platform was successfully designed for the sensitive detection of prostate-specific antigen (PSA), using reduced graphene oxide- functionalized iron oxyhydroxide (FeOOH-rGO) as the photoactive material, accompanying target-responsive controlled release system to achieve the signal amplification. Introduction of rGO as electron mediator greatly facilitated the electron transfer from FeOOH to electrode under visible light, which inhibited the electron-hole recombination to enhance the photo-activity of FeOOH-rGO. Additionally, the bioresponsive release system was controlled via the reaction of target PSA with the aptamer capped glucose-loading mesoporous silica nanoparticle (MSN) to release numerous glucose molecules (as the electron donors) for the amplification of the photocurrent generated from FeOOH-rGO. Thus, more glucose molecules could be released and enhanced photocurrents could be obtained with the increasing PSA concentrations. Experimental results showed that the photocurrents of the PEC sensing platform were linearly dependent on the logarithm of PSA concentrations from 1.0pg/mL to 100ng/mL. Moreover, the PEC sensing system afforded good stability and specificity, and its accuracy matched well with the commercial PSA enzyme-linked immunosorbent assay (ELISA) kit. The excellent performance of the PEC sensing platform indicated its promising prospect as a useful tool for PSA detection in practical application.

2073 related Products with: Reduced graphene oxide-functionalized FeOOH for signal-on photoelectrochemical sensing of prostate-specific antigen with bioresponsive controlled release system.

PSA (Prostate Specific A PSA (Prostate Specific A PSA (Prostate Specific A PSA (Prostate Specific A PSA (Prostate Specific A PSA (Prostate Specific A MOUSE ANTI BORRELIA BURGD Mouse Anti-Prostate Speci Human Prostate Specific A Prostate cancer, hyperpla Prostate-Specific Antigen PSAP (Prostate Specific

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#28494534   2017/05/11 Save this To Up

Ferroptosis: A Novel Anti-Tumor Action for Cisplatin.

Ferroptosis is a new mode of regulated cell death, which is completely distinct from other cell death modes based on morphological, biochemical, and genetic criteria. This study evaluated the therapeutic role of ferroptosis in classic chemotherapy drugs, including the underlying mechanism.

1786 related Products with: Ferroptosis: A Novel Anti-Tumor Action for Cisplatin.

MOUSE ANTI BOVINE ROTAVIR MOUSE ANTI BORRELIA BURGD RABBIT ANTI GSK3 BETA (pS Multiple organ tumor and Rabbit Anti-WT-1 Wilms Tu Rabbit Anti-WT-1 Wilms Tu Rabbit Anti-WT-1 Wilms Tu Rabbit Anti-WT-1 Wilms Tu Rabbit Anti-WT-1 Wilms Tu Rabbit Anti-WT-1 Wilms Tu Rabbit Anti-WT-1 Wilms Tu Rabbit Anti-WT-1 Wilms Tu

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#28351895   2017/03/29 Save this To Up

Magnetic poly(2-hydroxyethyl methacrylate) microspheres for affinity purification of monospecific anti-p46 kDa/Myo1C antibodies for early diagnosis of multiple sclerosis patients.

The aim of the present study is to develop new magnetic polymer microspheres with functional groups available for easy protein and antibody binding. Monodisperse macroporous poly(2-hydroxyethyl methacrylate) (PHEMA-COOH) microspheres ~4 µm in diameter and containing ∼1 mmol COOH/g were synthesized by multistep swelling polymerization of 2-hydroxyethyl methacrylate (HEMA), ethylene dimethacrylate (EDMA), and 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA), which was followed by MCMEMA hydrolysis. The microspheres were rendered magnetic by precipitation of iron oxide inside the pores, which made them easily separable in a magnetic field. Properties of the resulting magnetic poly(2-hydroxyethyl methacrylate) (mgt.PHEMA) particles with COOH functionality were examined by scanning and transmission electron microscopy (SEM and TEM), static volumetric adsorption of helium and nitrogen, mercury porosimetry, Fourier transform infrared (FTIR) and atomic absorption spectroscopy (AAS), and elemental analysis. Mgt.PHEMA microspheres were coupled with p46/Myo1C protein purified from blood serum of multiple sclerosis (MS) patients, which enabled easy isolation of monospecific anti-p46/Myo1C immunoglobulin G (IgG) antibodies from crude antibody preparations of mouse blood serum. High efficiency of this approach was confirmed by SDS/PAGE, Western blot, and dot blot analyses. The newly developed mgt.PHEMA microspheres conjugated with a potential disease biomarker, p46/Myo1C protein, are thus a promising tool for affinity purification of antibodies, which can improve diagnosis and treatment of MS patients.

1642 related Products with: Magnetic poly(2-hydroxyethyl methacrylate) microspheres for affinity purification of monospecific anti-p46 kDa/Myo1C antibodies for early diagnosis of multiple sclerosis patients.

MOUSE ANTI BOVINE ROTAVIR MOUSE ANTI BORRELIA BURGD RABBIT ANTI GSK3 BETA (pS Mouse Anti-Human Cytokera Mouse Anti-EBV Early Nucl Rat Anti-Mouse Forssman G Mouse Anti-HSV-6 Human Ea Mouse Anti-Human poly(ADP Mouse Anti-Human Cytokera Multiple Anti-IgG1 [+FITC Multiple Anti-IgG1(+FITC) Rabbit Anti-Poly-penicill

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#28341633   2017/03/25 Save this To Up

Increased hypoxia-inducible factor-1α in striated muscle of tumor-bearing mice.

Cancer cachexia is a progressive wasting disease resulting in significant effects on the quality of life and high mortality. Most studies on cancer cachexia have focused on skeletal muscle; however, the heart is now recognized as a major site of cachexia-related effects. To elucidate possible mechanisms, a proteomic study was performed on the left ventricles of colon-26 (C26) adenocarcinoma tumor-bearing mice. The results revealed several changes in proteins involved in metabolism. An integrated pathway analysis of the results revealed a common mediator in hypoxia-inducible factor-1α (HIF-1α). Work by other laboratories has shown that extensive metabolic restructuring in the C26 mouse model causes changes in gene expression that may be affected directly by HIF-1α, such as glucose metabolic genes. M-mode echocardiography showed progressive decline in heart function by day 19, exhibited by significantly decreased ejection fraction and fractional shortening, along with posterior wall thickness. Using Western blot analysis, we confirmed that HIF-1α is significantly upregulated in the heart, whereas there were no changes in its regulatory proteins, prolyl hydroxylase domain-containing protein 2 (PHD2) and von Hippel-Lindau protein (VHL). PHD2 requires both oxygen and iron as cofactors for the hydroxylation of HIF-1α, marking it for ubiquination via VHL and subsequent destruction by the proteasome complex. We examined venous blood gas values in the tumor-bearing mice and found significantly lower oxygen concentration compared with control animals in the third week after tumor inoculation. We also examined select skeletal muscles to determine whether they are similarly affected. In the diaphragm, extensor digitorum longus, and soleus, we found significantly increased HIF-1α in tumor-bearing mice, indicating a hypoxic response, not only in the heart, but also in skeletal muscle. These results indicate that HIF-1α may contribute, in part, to the metabolic changes that occur during cancer cachexia.NEW & NOTEWORTHY We used proteomics and metadata analysis software to identify contributors to metabolic changes in striated muscle during cancer cachexia. We found increased expression of hypoxia-inducible factor-1α in the heart and skeletal muscle, suggesting a potential target for the therapeutic treatment of cancer cachexia.

2990 related Products with: Increased hypoxia-inducible factor-1α in striated muscle of tumor-bearing mice.

ELISA Kit for Tumor Necr AKT1 (dn) Inducible Insulin promoter factor 1 Human Tumor Necrosis Fact Human Insulin-like Growth Human Migration Inhibitor Human Interleukin-1-alpha Human Insulin-like Growth Human Tumor Necrosis Fact Macrophage Colony Stimula Macrophage Colony Stimula TNFRSF1B - Goat polyclona

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#28315424   2017/03/18 Save this To Up

Diagnostic criteria for oncocytic renal neoplasms: a survey of urologic pathologists.

Renal oncocytoma and chromophobe renal cell carcinoma have been long recognized as distinct tumors; however, it remains unknown if uniform diagnostic criteria are used to distinguish these tumor types in practice. A survey was distributed to urologic pathologists regarding oncocytic tumors. Responses were received from 17 of 26 invitees. Histologically, more than 1 mitotic figure was regarded as most worrisome (n=10) or incompatible (n=6) with oncocytoma diagnosis. Interpretation of focal nuclear wrinkling, focal perinuclear clearing, and multinucleation depended on extent and did not necessarily exclude oncocytoma if minor. Staining techniques most commonly used included the following: cytokeratin 7 (94%), KIT (71%), vimentin (65%), colloidal iron (59%), CD10 (53%), and AMACR (41%). Rare cytokeratin 7-positive cells (≤5%) were regarded as most supportive of oncocytoma, although an extent excluding oncocytoma was not universal. Multiple chromosomal losses were most strongly supportive for chromophobe renal cell carcinoma diagnosis (65%). Less certainty was reported for chromosomal gain or a single loss. For tumors with mixed or inconclusive features, many participants use an intermediate diagnostic category (82%) that does not label the tumor as unequivocally benign or malignant, typically "oncocytic neoplasm" or "tumor" with comment. The term "hybrid tumor" was used variably in several scenarios. A slight majority (65%) report outright diagnosis of oncocytoma in needle biopsies. The morphologic, immunohistochemical, and genetic characteristics that define oncocytic renal tumors remain incompletely understood. Further studies correlating genetics, behavior, and histology are needed to define which tumors truly warrant classification as carcinomas for patient counseling and follow-up strategies.

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MOUSE ANTI BOVINE ROTAVIR Bone Morphogenetic Protei Growth Differentiation Fa Amplite™ Fluorimetric F MOUSE ANTI BORRELIA BURGD succinate-CoA ligase, GDP formin-like 1 antibody So succinate-CoA ligase, ADP Primary antibody Caspase Primary antibody FLIP An Glucose Assay With the La Cultrex In Vitro Angiogen

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#28247036   2017/03/01 Save this To Up

Ferritin heavy chain as a molecular imaging reporter gene in glioma xenografts.

The development of glioma therapy in clinical practice (e.g., gene therapy) calls for efficiently visualizing and tracking glioma cells in vivo. Human ferritin heavy chain is a novel gene reporter in magnetic resonance imaging. This study proposes hFTH as a reporter gene for MR molecular imaging in glioma xenografts.

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Amplite™ Luciferase Rep Amplite™ Luciferase Rep Amplite™ Luciferase Rep Amplite™ Gaussia Lucife Amplite™ Gaussia Lucife Amplite™ Renilla Lucife Amplite™ Renilla Lucife Amplite™ Renilla Lucife anti Ferritin Heavy chain SuperLight™ Dual Lucife SuperLight™ Luciferase SuperLight™ Luciferase

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#28230785   2017/02/23 Save this To Up

The Application of a Modified d-ROMs Test for Measurement of Oxidative Stress and Oxidized High-Density Lipoprotein.

Reactive oxygen species (ROS) are involved in the initiation and progression of atherosclerosis. ROS-derived hydroperoxides, as an indicator of ROS production, have been measured by using the diacron reactive oxygen metabolites (d-ROMs) test, which requires iron-containing transferrin in the reaction mixture. In this study we developed a modified d-ROMs test, termed the Fe-ROMs test, where iron ions were exogenously added to the reaction mixture. This modification is expected to exclude the assay variation that comes from different blood iron levels in individuals. In addition, this Fe-ROMs test was helpful for determining the class of plasma lipoproteins that are hydroperoxidized. Low-density lipoprotein/very low-density lipoprotein (LDL/VLDL) and high-density lipoprotein (HDL) were purified by use of an LDL/VLDL purification kit and the dextran sulfate-Mg(2+) precipitation method, respectively; their hydroperoxide contents were assessed by performing the Fe-ROMs test. The majority of the hydroperoxides were detected only in the HDL fraction, not in the LDL/VLDL. Further detailed analysis of HDLs by size-exclusion high-performance liquid chromatography revealed that the hydroperoxide-containing molecules were small-sized HDLs. Because HDL was shown to be the principal vehicle for the plasma hydroperoxides, this Fe-ROMs test is a beneficial method for the assessment of oxidized-HDL levels. Indeed, Fe-ROMs levels were strongly associated with the levels of oxidized HDL, which were determined by performing the malondialdehyde-modified HDL enzyme immunoassay. In conclusion, the Fe-ROMs test using plasma itself or the HDL fraction after dextran sulfate-Mg(2+) precipitation is useful to assess the functionality of HDL, because the oxidation of HDL impairs its antiatherogenic capacity.

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OXI TEK (Oxidative Stress Rabbit Anti-HDL high dens Rabbit Anti-HDL high dens Rabbit Anti-HDL high dens Rabbit Anti-HDL high dens Rabbit Anti-HDL high dens Rabbit Anti-HDL high dens Rabbit Anti-HDL high dens Rabbit Anti-HDL high dens Rabbit Anti-HDL high dens Rabbit Anti-HDL high dens Rabbit Anti-HDL high dens

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#28189796   2017/02/12 Save this To Up

Substrate binding in human indoleamine 2,3-dioxygenase 1: A spectroscopic analysis.

Human indoleamine 2,3-dioxygenase (hIDO1) is a heme enzyme that catalyzes the oxidative cleavage of the L-tryptophan indole ring. As increased levels of hIDO1 expression in tumor cells correlate with a poor prognosis for surviving several cancer types, hIDO1 has become an appealing drug target for cancer therapy. However, detailed structural knowledge of the catalytically active complex is necessary to eb able to design de novo inhibitors selective for hIDO1. Here we have applied Fourier transform infrared (FTIR) and nanosecond time-resolved optical spectroscopy to hIDO1 variants with modified heme pocket structures to identify important amino acid residues that stabilize the substrate in the active site. A cluster of small side chain residues at positions 260-265 ensures structural flexibility of the binding site. Thr379 and Arg231 are key residues acting in concert to bind the substrate. Thr379 is the final residue of a disordered loop; the neighboring Gly380, however, is still visible in the X-ray structure of the substrate-free protein, 20Å away from the heme iron. Therefore, large-scale conformational changes are necessary to bring Thr379 close to the substrate. The use of substrate analogs further reveals that an indole-like side chain with two aromatic rings and L-stereoisomery at the Cα are required for high affinity binding.

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Sheep Anti-Human Indoleam Goat Anti-Human Vitamin D Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Human Macrophage Inflamma Human Macrophage Inflamma Human Interleukin-32 alph Human Interleukin-1-alpha Interferon-a Receptor Typ Goat Anti-Human Synaptota Goat Anti-Human STK39 SPA Goat Anti-Human SPHK1, (i

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#28144141   2017/02/01 Save this To Up

In vitro toxicity of FemOn, FemOn-SiO2 composite, and SiO2-FemOn core-shell magnetic nanoparticles.

Over the last decade, magnetic iron oxide nanoparticles (IONPs) have drawn much attention for their potential biomedical applications. However, serious in vitro and in vivo safety concerns continue to exist. In this study, the effects of uncoated, FemOn-SiO2 composite flake-like, and SiO2-FemOn core-shell IONPs on cell viability, function, and morphology were tested 48 h postincubation in human umbilical vein endothelial cell culture. Cell viability and apoptosis/necrosis rate were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and annexin V-phycoerythrin kit, respectively. Cell morphology was evaluated using bright-field microscopy and forward and lateral light scattering profiles obtained with flow cytometry analysis. All tested IONP types were used at three different doses, that is, 0.7, 7.0, and 70.0 μg. Dose-dependent changes in cell morphology, viability, and apoptosis rate were shown. At higher doses, all types of IONPs caused formation of binucleated cells suggesting impaired cytokinesis. FemOn-SiO2 composite flake-like and SiO2-FemOn core-shell IONPs were characterized by similar profile of cytotoxicity, whereas bare IONPs were shown to be less toxic. The presence of either silica core or silica nanoflakes in composite IONPs can promote cytotoxic effects.

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Cultrex In Vitro Angiogen Human integrin aVb3, affi Colon cancer high density Inhibitory Mouse Monoclon Inhibitory Mouse Monoclon High density larynx and p Resorufin Oleate, Fluorog FIV Core Ag, recombinant Small intestine cancer, m Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti Hepatitis B Core Antigen

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#28140480   2017/01/31 Save this To Up

Acute-Phase Proteins and Iron Status in Cats with Chronic Kidney Disease.

The role of inflammation in the development and progression of chronic kidney disease (CKD) in cats is not well characterized. Hepcidin is a recently discovered acute-phase protein (APP) that plays an important role in iron metabolism and contributes to the development of anemia in humans with CKD.

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Anti 3 DG imidazolone Mon Kidney disease spectrum ( Syringe pump can be contr Native Influenza HA (A Br Native Influenza HA (A Br Native Influenza HA (A Br Native Influenza HA (A Ca Native Influenza HA (A Ca Native Influenza HA (A Ca Recombinant Influenza HA Recombinant Influenza HA Recombinant Influenza HA

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