Search results for: Laminar Flow Cabinet BBSDDS Horizontal
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Aseptic technique for cell culture.
This unit describes some of the ways that a laboratory can deal with the constant threat of microbial contamination in cell cultures. A protocol on aseptic technique is described first. This catch-all term universally appears in any set of instructions pertaining to procedures in which noncontaminating conditions must be maintained. In reality, aseptic technique encompasses all aspects of environmental control, personal hygiene, equipment and media sterilization, and associated quality control procedures needed to ensure that a procedure is, indeed, performed with aseptic, noncontaminating technique. Although cell culture can theoretically be carried out on an open bench in a low-traffic area, most cell culture work is carried out using a horizontal laminar-flow clean bench or a vertical laminar-flow biosafety cabinet. Both are described here.R J Coté
2362 related Products with: Aseptic technique for cell culture.
10 lt 1KG1 kit(96 Wells)50 g50 ml5 x 2 mlcase1 kit(96 Wells)10 ml1 kit(96 Wells)
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Biological safety cabinetry.
The biological safety cabinet is the one piece of laboratory and pharmacy equipment that provides protection for personnel, the product, and the environment. Through the history of laboratory-acquired infections from the earliest published case to the emergence of hepatitis B and AIDS, the need for health care worker protection is described. A brief description with design, construction, function, and production capabilities is provided for class I and class III safety cabinets. The development of the high-efficiency particulate air filter provided the impetus for clean room technology, from which evolved the class II laminar flow biological safety cabinet. The clean room concept was advanced when the horizontal airflow clean bench was manufactured; it became popular in pharmacies for preparing intravenous solutions because the product was protected. However, as with infectious microorganisms and laboratory workers, individual sensitization to antibiotics and the advent of hazardous antineoplastic agents changed the thinking of pharmacists and nurses, and they began to use the class II safety cabinet to prevent adverse personnel reactions to the drugs. How the class II safety cabinet became the mainstay in laboratories and pharmacies is described, and insight is provided into the formulation of National Sanitation Foundation standard number 49 and its revisions. The working operations of a class II cabinet are described, as are the variations of the four types with regard to design, function, air velocity profiles, and the use of toxins. The main certification procedures are explained, with examples of improper or incorrect certifications. The required levels of containment for microorganisms are given. Instructions for decontaminating the class II biological safety cabinet of infectious agents are provided; unfortunately, there is no method for decontaminating the cabinet of antineoplastic agents.R H Kruse, W H Puckett, J H Richardson
2701 related Products with: Biological safety cabinetry.
10 ug5ml2ml5ml50ml50ml10ml100ml10ml50 ug
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Contamination of cultures processed with the isolator lysis-centrifugation blood culture tube.
Overall contamination (on- plus off-streak) of the Isolator (Du Pont Co.) blood culture tube (23%) was greater than that of a conventional broth blood culture bottle (0.6%) or that of a biphasic blood culture bottle (1.3%). To determine the source of this contamination, Isolator cultures of blood from 59 healthy volunteers and of sterile broth from 60 vials were made. A total of 37% of the blood cultures and 22% of the broth cultures were contaminated (P = 0.06). Staphylococcus epidermidis-contaminated cultures represented 31 and 10% of the blood and broth cultures, respectively (P = 0.06). Contamination of plates processed on a bench top, in front of horizontal laminar flow, and in a biological safety cabinet with vertical laminar flow were compared. Processing plates in a biological safety cabinet resulted in a significant reduction in the number of contaminated plates (P less than 0.05). The contamination rate for 7,874 Isolator blood cultures processed in the biological safety cabinet was significantly decreased to 6.7% on-streak (9.3% on- plus off-streak). Contamination of Isolator-processed blood cultures originated from the laboratory and the patient. The former can be reduced by inoculating plates in a vertical laminar flow biological safety cabinet and by maintaining adequate quality control of media. The latter may be unavoidable.R B Thomson, S J Vanzo, N K Henry, K L Guenther, J A Washington
2103 related Products with: Contamination of cultures processed with the isolator lysis-centrifugation blood culture tube.
100 extractions10x96, 2.0ml cultures50 samplescultured cells (100 ml)cultured cells (50 ml)1 kit(96 Wells)100 extractionsmin 2 cartonsUp to 200 ml cultures100 samples
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Prevention of airborne contamination and cross-contamination in germ-free mice by laminar flow.
The efficacy of horizontal and vertical laminar flow units (equipped with high-efficiency air filters) in the prevention of cross-contamination between cages and of contamination from outside has been demonstrated. With germ-free mice and using germ-free standard techniques for sterilization and for the transfer of germ-free mice into the cabinets via a standard entry lock, it was found that during an observation period of 2 weeks the animals remain ;negative'. Other experiments were performed with equally good results in cabinets equipped with a hinged flap, closing 95% of the open front side. When the flap was closed the air flow could be reduced accordingly, thus reducing the noise level and the risk of dehydration.Experiments made with germ-free mice in a ;down-flow unit' were also invariably good.In another type of experiment, cages with conventional mice were placed in the cabinets between cages with germ-free animals at varying distances. If all animals were maintained on wire mesh (to minimize the aerosol production of dust) and if the ;conventional' cages were at a distance of 10 cm. from ;germ-free cages' the latter remained bacteria-free during test periods of one week.The use of ;laminar flow isolators' for the isolation of human patients is mentioned.D van der Waaij, A H Andreas