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[Modification of a panoptic method of staining isolated cells].

A simple and rapid method of isolated cell staining was achieved by modification of the Pappenheim's method. It is based on the following steps: (1) dilution of commonly used stock solutions May-Grünwald (1:1), Giemsa-Romanowsky (1.25% v/v), (2) reverse sequence of the used staining solutions in comparison with the original method, (3) shorter time of procedure (5 min/slide), (4) utilization of distilled water of pH 5.1-5.6 for dilution of solutions and rinsing of slides, (5) it is not necessary to add serum to cell suspensions. The presented histological method is suitable for cytomorphological examination of cells. It can be applied in routine hematological laboratories not equipped with cytospins. (Fig. 5, Ref. 6.).
E Paulíková, A Kociková, M Sabol

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100tests100ul100 ul100tests100.00 ug 70 Slides 25 assays 100ul200 500 Slides

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Staining properties and stability of a standardised Romanowsky stain.

An evaluation of the standardised Romanowsky stain of Marshall et al. has been made in a routine haematology laboratory. It was noted that this stain had several advantages over the May-Grünwald Giemsa stain used in most British laboratories. These advantages include ease and speed of preparation, a shorter staining time, and reproducibility of results. These results are described in detail. The stability of the stock stain solution and of the 'working' stain (stock + buffer) has been studied by, respectively, thin-layer chromatography and visible spectroscopy. No change was detected in the composition of the stock solution at ambient temperature over a period of six months. Stability was unaffected by the composition of the container (polyethylene, PyrexTM, or soda-glass) or by daylight. The 'working' solution was stable for 3 hours. Thereafter a precipitate is formed, consisting of thiazine dyes and eosin in a molar ratio of approximately 2:1.
P N Marshall, S A Bentley, S M Lewis

2740 related Products with: Staining properties and stability of a standardised Romanowsky stain.

1 kit(s) 96 wells (1 kit) 70 Slides 0,5 ML100ug100 assays 500 Slides 0,1 ML25 assays 70 Slides 1 g2.5 mg

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