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Search results for: Monoclonal Anti-BAF155 produced in mouse Antibody

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#38499218   2024/03/16 To Up

Inducible IL-2 production and IL-2 cell expansion are landmark events for T-cell activation of teleost.

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Jiansong Zhang, Kang Li, Yi Cao, Ding Wang, Jie Cheng, Haiyou Gao, Ming Geng, Jialong Yang, Xiumei Wei

1579 related Products with: Inducible IL-2 production and IL-2 cell expansion are landmark events for T-cell activation of teleost.

500 ml25ml25ml2500 assays5 x 200ul/Unit25ml2500 assays

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#38490439   2024/03/13 To Up

Extracellular metallothionein as a therapeutic target in the early progression of Type 1 Diabetes.

Type 1 diabetes (T1D) is characterized by lymphocyte infiltration into the pancreatic islets of Langerhans, leading to the destruction of insulin-producing beta cells and uncontrolled hyperglycemia. In the non-obese diabetic (NOD) murine model of T1D, onset of this infiltration starts several weeks before glucose dysregulation and overt diabetes. Recruitment of immune cells to the islets is mediated by several chemotactic cytokines, including CXCL10, while other cytokines, including SDF-1α, can confer protective effects. Global gene expression studies of the pancreas from prediabetic NOD mice and single cell sequence analysis of human islets from prediabetic, autoantibody positive patients showed an increased expression of metallothionein (MT), a small molecular weight, cysteine-rich metal binding stress response protein. We have shown that beta cells can release MT into the extracellular environment, which can subsequently enhance the chemotactic response of Th1 cells to CXCL10 and interfere with the chemotactic response of Th2 cells to SDF-1α. These effects can be blocked in vitro with a monoclonal anti-MT antibody, clone UC1MT. When administered to NOD mice before the onset of diabetes, UC1MT significantly reduces the development of T1D. Manipulation of extracellular MT may be an important approach to preserving beta cell function and preventing the development of T1D.
Clare K Melchiorre, Matthew D Lynes, Sadikshya Bhandari, Sheng-Chiang Su, Christian M Potts, Amy V Thees, Carol E Norris, Lucy Liaw, Yu-Hua Tseng, Michael A Lynes

1067 related Products with: Extracellular metallothionein as a therapeutic target in the early progression of Type 1 Diabetes.

0.1ml (1mg/ml)100ug Lyophilized 100ul1000 assays100ug

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#38489115   2024/03/15 To Up

Modulation of T Cell Differentiation in Mice with COPD Combined with Lung Cancer Through Key Targets of PD-1 by Tao Hong Si Wu Tang.

The objective is to assess the anti-inflammatory effect of Tao Hong Si Wu Tang combined with anti-PD-1 in a mouse model of COPD combined with lung cancer, elucidating its mechanism through modulation of PD-1/PD-L binding, regulation of Th1/Th2 and Th17/Treg balance, inhibition of IL-4 and IL-17, and promotion of IFN-γ and TGF-β levels in peripheral blood. One hundred male C57/BL6 mice were randomly allocated to five groups: A (blank control), B (model control), C (THSW), D (anti-PD-1), and E (THSW + anti-PD-1), with 20 mice in each group. The COPD model was induced using fumigation and LPS intra-airway drip, followed by the establishment of lung cancer by Lewis cell inoculation. Treatment groups received Tao Hong Si Wu Tang or/and PD-1 monoclonal antibody. Various indicators were assessed, including macroscopic observation, HE staining of lung tissue, ELISA for cytokines, flow cytometry for cell proportions, and immunohistochemistry/western blotting for protein expression. Lung tissue analysis revealed significant differences between groups, with marked tumor formation observed in groups B-E. Serum levels of IL-4, IFN-γ, IL-17, and TGF-β were significantly altered, along with changes in CD4 + T/CD8 + T ratio and cytokine-producing cell populations. Expression levels of key proteins were also significantly affected across treatment groups. Tao Hong Si Wu Tang demonstrated anti-inflammatory effects comparable to anti-PD-1, potentially through modulation of PD-1/PD-L binding, correction of Th1/Th2 and Th17/Treg imbalance, and modulation of cytokine levels. These findings suggest a role for Tao Hong Si Wu Tang in ameliorating inflammation and immune dysregulation in COPD combined with lung cancer.
Guoli Wang, Ge Wang, Keming Zhao, Aifeng Sui, Lina Wang, Yanling Xu, Nini Qu, Xiande Ma, Hu Deng

2433 related Products with: Modulation of T Cell Differentiation in Mice with COPD Combined with Lung Cancer Through Key Targets of PD-1 by Tao Hong Si Wu Tang.



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#38467979   // To Up

Development of Orally Ingestible IgA Antibody Drugs to Maintain Symbiosis Between Humans and Microorganisms.

In recent years, dysbiosis, abnormalities in the gut microbiota, has been reported to be associated with the development of many diseases, and improving the gut microbiota is important for health maintenance. It has been shown that the host recognizes and regulates intestinal bacteria by means of IgA antibodies secreted into the gut, but the precise nature of the commensal gut bacteria recognized by each IgA antibody is unclear. We have cloned monoclonal IgA antibodies from mouse intestinal IgA-producing cells and are searching for bacterial molecules recognized by each IgA clone. Although the interaction of IgA antibodies with intestinal bacteria is still largely unknown and requires further basic research, we discuss the potential use of orally ingestible IgA antibodies as agents to improve intestinal microbiota.
Reiko Shinkura

1440 related Products with: Development of Orally Ingestible IgA Antibody Drugs to Maintain Symbiosis Between Humans and Microorganisms.

1 module200ul200 ug1 module1 module1 module1 module100 ug1 module1 module1 module200 ug

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#38446855   2024/03/06 To Up

Central tolerance shapes the neutralizing B cell repertoire against a persisting virus in its natural host.

Viral mimicry of host cell structures has been postulated to curtail the B cell receptor (BCR) repertoire against persisting viruses through tolerance mechanisms. This concept awaits, however, experimental testing in a setting of natural virus-host relationship. We engineered mouse models expressing a monoclonal BCR specific for the envelope glycoprotein of lymphocytic choriomeningitis virus (LCMV), a naturally persisting mouse pathogen. When the heavy chain of the LCMV-neutralizing antibody KL25 was paired with its unmutated ancestor light chain, most B cells underwent receptor editing, a behavior reminiscent of autoreactive clones. In contrast, monoclonal B cells expressing the same heavy chain in conjunction with the hypermutated KL25 light chain did not undergo receptor editing but exhibited low levels of surface IgM, suggesting that light chain hypermutation had lessened KL25 autoreactivity. Upon viral challenge, these IgM cells were not anergic but up-regulated IgM, participated in germinal center reactions, produced antiviral antibodies, and underwent immunoglobulin class switch as well as further affinity maturation. These studies on a persisting virus in its natural host species suggest that central tolerance mechanisms prune the protective antiviral B cell repertoire.
Marianna Florova, Tiago Abreu-Mota, Guido C Paesen, Anna Sophia Beetschen, Karen Cornille, Anna-Friederike Marx, Kerstin Narr, Mehmet Sahin, Mirela Dimitrova, Nivedya Swarnalekha, Jane Beil-Wagner, Natasa Savic, Pawel Pelczar, Thorsten Buch, Carolyn G King, Thomas A Bowden, Daniel D Pinschewer

2980 related Products with: Central tolerance shapes the neutralizing B cell repertoire against a persisting virus in its natural host.

25 ml.0.1ml (1mg/ml)1 L.1mg0.1ml100ug Lyophilized1100 ulcase1100 ul

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#38387059   2024/02/22 To Up

Cell-surface ZnT8 antibody prevents and reverses autoimmune diabetes in mice.

Type 1 diabetes (T1D) is an autoimmune disease where pathogenic lymphocytes target autoantigens expressed in the pancreatic islets, leading to the destruction of insulin-producing β-cells. Zinc transporter 8 (ZnT8) is a major autoantigen abundantly present on the β-cell surface. This unique molecular target offers the potential to shield β-cells against autoimmune attacks in T1D. Our previous work showed that a monoclonal antibody (mAb43) against cell-surface ZnT8 can home in on the pancreatic islets and prevent autoantibodies from recognizing β-cells. This study demonstrates that mAb43 binds to exocytotic sites on the β-cell surface, masking the antigenic exposure of ZnT8 and insulin following glucosestimulated insulin secretion. In vivo administration of mAb43 to nonobese diabetic (NOD) mice selectively increased the proportion of regulatory T-cells (Tregs) in the islet, resulting in complete and sustained protection against T1D onset as well as reversal of new-onset diabetes. The mAb43-induced self-tolerance was reversible after treatment cessation and exhibited no adverse effects during long-term monitoring. Our findings suggest that mAb43 masking of the antigenic exposure of β-cells suppresses the immunological cascade from B-cell antigen presentation to T-cell mediated β-cell destruction, providing a novel islet-targeted and antigen-specific immunotherapy to prevent and reverse clinical T1D.
Devi Kasinathan, Zheng Guo, Dylan C Sarver, G William Wong, Shumei Yun, Aaron W Michels, Liping Yu, Chandan Sona, Matthew N Poy, Maria L Golson, Dax Fu

2738 related Products with: Cell-surface ZnT8 antibody prevents and reverses autoimmune diabetes in mice.

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#38381847   2024/02/21 To Up

Synthetic development of a broadly neutralizing antibody against snake venom long-chain α-neurotoxins.

Snakebite envenoming is a major global public health concern for which improved therapies are urgently needed. The antigenic diversity present in snake venom toxins from various species presents a considerable challenge to the development of a universal antivenom. Here, we used a synthetic human antibody library to find and develop an antibody that neutralizes long-chain three-finger α-neurotoxins produced by numerous medically relevant snakes. Our antibody bound diverse toxin variants with high affinity, blocked toxin binding to the nicotinic acetylcholine receptor in vitro, and protected mice from lethal venom challenge. Structural analysis of the antibody-toxin complex revealed a binding mode that mimics the receptor-toxin interaction. The overall workflow presented is generalizable for the development of antibodies that target conserved epitopes among antigenically diverse targets, and it offers a promising framework for the creation of a monoclonal antibody-based universal antivenom to treat snakebite envenoming.
Irene S Khalek, R R Senji Laxme, Yen Thi Kim Nguyen, Suyog Khochare, Rohit N Patel, Jordan Woehl, Jessica M Smith, Karen Saye-Francisco, Yoojin Kim, Laetitia Misson Mindrebo, Quoc Tran, Mateusz Kędzior, Evy Boré, Oliver Limbo, Megan Verma, Robyn L Stanfield, Stefanie K Menzies, Stuart Ainsworth, Robert A Harrison, Dennis R Burton, Devin Sok, Ian A Wilson, Nicholas R Casewell, Kartik Sunagar, Joseph G Jardine

2842 related Products with: Synthetic development of a broadly neutralizing antibody against snake venom long-chain α-neurotoxins.

100ug1 mL0.05 mg100ug Lyophilized100ug100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100 μg100ug100ug Lyophilized

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#38379712   2024/01/17 To Up

Validation of a fibrinogen γ' enzyme-linked immunosorbent assay using a new monoclonal antibody: effects of bariatric surgery.

Fibrinogen γ' is a naturally occurring 20-amino-acid splice variant of the fibrinogen γ chain. Animal studies link variations in fibrinogen to obesity, but it is unknown how fibrinogen γ' is associated with obesity in humans.
Nadja Bødker Pedersen, Else-Marie Bladbjerg, Claus Bogh Juhl, Anette Larsen, Anna-Marie Bloch Münster, Moniek P M de Maat, Yaseelan Palarasah

2373 related Products with: Validation of a fibrinogen γ' enzyme-linked immunosorbent assay using a new monoclonal antibody: effects of bariatric surgery.

0.1ml (1.0mg/ml)100ug Lyophilized1mg1 mg100ul1 mg100ug 50UG100ul

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