Search results for: Mouse Anti-Chicken MHC Class II Antibodies
#30626692 
2019/01/09
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Characterization of Subpopulations of Chicken Mononuclear Phagocytes That Express TIM4 and CSF1R.
The phosphatidylserine receptor TIM4, encoded by , mediates the phagocytic uptake of apoptotic cells. We applied anti-chicken TIM4 mAbs in combination with reporter transgenes to dissect the function of TIM4 in the chick (). During development in ovo, TIM4 was present on the large majority of macrophages, but expression became more heterogeneous posthatch. Blood monocytes expressed KUL01, class II MHC, and mApple uniformly. Around 50% of monocytes were positive for surface TIM4. They also expressed many other monocyte-specific transcripts at a higher level than TIM4 monocytes. In liver, highly phagocytic TIM4 cells shared many transcripts with mammalian Kupffer cells and were associated with uptake of apoptotic cells. Although they expressed mRNA, Kupffer cells did not express the -mApple transgene, suggesting that additional transcriptional regulatory elements are required by these cells. By contrast, -mApple was detected in liver TIM4 and TIM4 cells, which were not phagocytic and were more abundant than Kupffer cells. These cells expressed alongside high levels of , , , and other markers associated with conventional dendritic cells in mice. In bursa, TIM4 was present on the cell surface of two populations. Like Kupffer cells, bursal TIM4 phagocytes coexpressed many receptors involved in apoptotic cell recognition. TIM4 cells appear to be a subpopulation of bursal B cells. In overview, TIM4 is associated with phagocytes that eliminate apoptotic cells in the chick. In the liver, TIM4 and reporters distinguished Kupffer cells from an abundant population of dendritic cell-like cells.Tuanjun Hu, Zhiguang Wu, Stephen J Bush, Lucy Freem, Lonneke Vervelde, Kim M Summers, David A Hume, Adam Balic, Pete Kaiser
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Immunolocalization of MHC-II+ cells in the ovary of immature, young laying and old laying hens Gallus domesticus.
The aim of this study was to localize major histocompatibility complex class II positive (MHC-II+) cells in the hen ovary, and to determine the effects of ageing and sex steroids on their frequency. Cryostat sections of ovarian tissues of immature, young laying and old laying hens and those of immature hens treated with or without diethylstilboestrol or progesterone were prepared. Sections were immunostained for MHC class II antigens using mouse anti-chicken MHC class II monoclonal antibody and observed under a light microscope. Positive cells were counted using a computer-assisted image analyser. MHC-II+ cells were localized in the ovarian stroma and theca layer of primary follicles in all birds examined. The frequency of MHC-II+ cells in the stroma and theca of primary follicles (approximately 400-600 microns in diameter) was significantly greater in young laying hens than it was in immature and old laying hens (P < 0.01). In the stroma and the theca of primary follicles of diethylstilboestrol-treated birds, the frequency of MHC-II+ cells was significantly greater than it was in the stroma and theca of control and progesterone-treated birds (P < 0.01). Progesterone had no significant effect when compared with controls. These results indicate that both the ovarian stroma and theca of follicles in the hen ovary contain MHC-II+ cells, the frequency of MHC-II+ cells increases in association with sexual maturation and decreases thereafter during ageing, and oestrogen may be one of the factors enhancing the induction of MHC-II+ cells in the ovary.A Barua, Y Yoshimura