Search results for: Mouse Anti-GAPDH(3E12)-Loading Control Monoclonal Antibody, PE-Cy5.5 conjugated Isotype: IgG
#29724483 // Save this To Up
Immune suppression of food allergy by maternal IgG in murine models.Most of the patients develop food allergy early in life. The factors related to parental immune condition might be one of the conceivable causes.
anti-Diazepam Binding Inh Mouse Anti Shigella boydi Mouse Anti P. aeruginosa Mouse Anti Salmonella typ Hamster AntiSerine Protea anti HCMV IE pp65 IgG1 (m Mouse AntiInfluenza A Tar anti-C1 inhibitor (4G12), Mouse AntiMRSA Target Ant Mouse Anti-Insulin-Like G HIV1 integrase antibody, DMPO, N1664A Host Mouse S
#26661181 // Save this To Up
Internalization of targeted quantum dots by brain capillary endothelial cells in vivo.Receptors located on brain capillary endothelial cells forming the blood-brain barrier are the target of most brain drug delivery approaches. Yet, direct subcellular evidence of vectorized transport of nanoformulations into the brain is lacking. To resolve this question, quantum dots were conjugated to monoclonal antibodies (Ri7) targeting the murine transferrin receptor. Specific transferrin receptor-mediated endocytosis of Ri7-quantum dots was first confirmed in N2A and bEnd5 cells. After intravenous injection in mice, Ri7-quantum dots exhibited a fourfold higher volume of distribution in brain tissues, compared to controls. Immunofluorescence analysis showed that Ri7-quantum dots were sequestered throughout the cerebral vasculature 30 min, 1 h, and 4 h post injection, with a decline of signal intensity after 24 h. Transmission electron microscopic studies confirmed that Ri7-quantum dots were massively internalized by brain capillary endothelial cells, averaging 37 ± 4 Ri7-quantum dots/cell 1 h after injection. Most quantum dots within brain capillary endothelial cells were observed in small vesicles (58%), with a smaller proportion detected in tubular structures or in multivesicular bodies. Parenchymal penetration of Ri7-quantum dots was extremely low and comparable to control IgG. Our results show that systemically administered Ri7-quantum dots complexes undergo extensive endocytosis by brain capillary endothelial cells and open the door for novel therapeutic approaches based on brain endothelial cell drug delivery.
1801 related Products with: Internalization of targeted quantum dots by brain capillary endothelial cells in vivo.Human Internal Mammary Ar GFP Expressing Human Brai Human Large Intestine Mic GFP Expressing Mouse Brai Human Brain Microvascular GFP Expressing Human Inte Human Small Intestine Mic MarkerGeneTM in vivo lacZ Mouse Brain Microvascular Directed In Vivo Angiogen Human Pancreatic Microvas Human Tonsil Microvascula
#25908833 // Save this To Up
Small-Animal PET Imaging of Pancreatic Cancer Xenografts Using a 64Cu-Labeled Monoclonal Antibody, MAb159.Overexpression of the GRP78 receptor on cell surfaces has been linked with tumor growth, metastasis, and resistance to therapy. We developed a (64)Cu-labeled probe for PET imaging of tumor GRP78 expression based on a novel anti-GRP78 monoclonal antibody, MAb159.
1096 related Products with: Small-Animal PET Imaging of Pancreatic Cancer Xenografts Using a 64Cu-Labeled Monoclonal Antibody, MAb159.Monoclonal Anti-Breast Ca Proteins and Antibodies H Anti-Infectious Pancreati Monoclonal Anti-Breast Ca Proteins and Antibodies H Monoclonal antibody to hu Anti-Infectious Pancreati Anti-Infectious Pancreati Anti-Infectious Pancreati Monoclonal antibody to hu Monoclonal Anti-C-Reactiv Rabbit Anti-F1 protein (Y
#24054660 // Save this To Up
Real-time and sensitive detection of Salmonella Typhimurium using an automated quartz crystal microbalance (QCM) instrument with nanoparticles amplification.The accidental contamination of Salmonella in raw and processed foods is a major problem for the food industry worldwide. At present many of the currently used methods for Salmonella detection are time and labour intensive. Therefore, rapid detection is a key to the prevention and identification of problems related to health and safety. This paper describes the application of a new quartz crystal microbalance (QCM) instrument with a microfluidic system for the rapid and real time detection of Salmonella Typhimurim. The QCMA-1 bare gold sensor chip which contain two sensing array was modified by covalently immobilising the monoclonal capture antibody on the active spot and a mouse IgG antibody on the control spot using a conventional amine coupling chemistry (EDC-NHS). The binding of the Salmonella cells onto the immobilised anti-Salmonella antibody alters the sensor frequency which was correlated to cells concentration in the buffer samples. Salmonella cells were detected using direct, sandwich, and sandwich assay with antibody conjugated gold-nanoparticles. The performance of the QCM immunosensor developed with gold-nanoparticles gave the highest sensitivity with a limit of detection (LOD) ~10-20 colony forming unit (CFU) ml(-1) compared to direct and sandwich assay (1.83 × 10(2) CFU ml(-1) and 1.01 × 10(2) CFU ml(-1), respectively). The sensor showed good sensitivity and selectivity for Salmonella in the presence of other bacteria in real food samples and helped in reducing the pre-enrichment step, hence, demonstrating the potential of this technology for the rapid and sensitive microbial analysis.
2716 related Products with: Real-time and sensitive detection of Salmonella Typhimurium using an automated quartz crystal microbalance (QCM) instrument with nanoparticles amplification.Salmonella Real Time PCR Rabbit Anti-Salmonella ty Rabbit Anti-Salmonella ty Rabbit Anti-Salmonella ty Rabbit Anti-Salmonella ty Salmonella Real Time PCR Rabbit Anti-Salmonella ty Rabbit Anti-Salmonella ty Rabbit Anti-Salmonella ty Syringe pump can be contr Rabbit Anti-Salmonella ty Mouse Anti-Salmonella typ
#23298904 // Save this To Up
Identification of the optimal therapeutic antibody for fluorescent imaging of cutaneous squamous cell carcinoma.Intraoperative, real-time fluorescence imaging may significantly improve tumor visualization and resection and postoperatively, in pathological assessment. To this end, we sought to determine the optimal FDA approved therapeutic monoclonal antibody for optical imaging of human cutaneous squamous cell carcinoma (cSCC). A near-infrared (NIR) fluorescent probe (IRDye800) was covalently linked to bevacizumab, panitumumab or tocilizumab and injected systemically into immunodeficient mice bearing either cutaneous tumor cell lines (SCC13) or cutaneous human tumor explants. Tumors were then imaged and resected under fluorescent guidance with the SPY, an FDA-approved intraoperative imaging system, and the Pearl Impulse small animal imaging system. All fluorescently labeled antibodies delineated normal tissue from tumor in SCC13 xenografts based on tumor-to-background (TBR) ratios. The conjugated antibodies produced TBRs of 1.2-2 using SPY and 1.6-3.6 using Pearl; in comparison, isotype control antibody IgG-IRDye produced TBRs of 1.0 (SPY) and 0.98 (Pearl). Comparison between antibodies revealed them to be roughly equivalent for imaging purposes with both the SPY and Pearl (p = 0.89 SPY, p = 0.99 Pearl; one way ANOVA). Human tumor explants were also imaged and tumor detection was highest with panitumumab-IRDye800 when using the SPY (TBR 3.0) and Pearl (TBR 4.0). These data suggest that FDA approved antibodies may be clinically used for intraoperative detection of cSCC.
2060 related Products with: Identification of the optimal therapeutic antibody for fluorescent imaging of cutaneous squamous cell carcinoma.Lung squamous cell carcin Human squamous cell carci FDA Standard Frozen Tissu Normal rat multiple organ Oral cavity squamous cell Multiple head and neck sq Esophageal squamous cell FDA Standard Frozen Tissu Lung squamous cell carcin Multiple organ squamous c Skin squamous cell carcin Esophagus squamous cell c
#22988081 // Save this To Up
Synthesis of site-specific antibody-drug conjugates using unnatural amino acids.Antibody-drug conjugates (ADCs) allow selective targeting of cytotoxic drugs to cancer cells presenting tumor-associated surface markers, thereby minimizing systemic toxicity. Traditionally, the drug is conjugated nonselectively to cysteine or lysine residues in the antibody. However, these strategies often lead to heterogeneous products, which make optimization of the biological, physical, and pharmacological properties of an ADC challenging. Here we demonstrate the use of genetically encoded unnatural amino acids with orthogonal chemical reactivity to synthesize homogeneous ADCs with precise control of conjugation site and stoichiometry. p-Acetylphenylalanine was site-specifically incorporated into an anti-Her2 antibody Fab fragment and full-length IgG in Escherichia coli and mammalian cells, respectively. The mutant protein was selectively and efficiently conjugated to an auristatin derivative through a stable oxime linkage. The resulting conjugates demonstrated excellent pharmacokinetics, potent in vitro cytotoxic activity against Her2(+) cancer cells, and complete tumor regression in rodent xenograft treatment models. The synthesis and characterization of homogeneous ADCs with medicinal chemistry-like control over macromolecular structure should facilitate the optimization of ADCs for a host of therapeutic uses.
1818 related Products with: Synthesis of site-specific antibody-drug conjugates using unnatural amino acids.anti GFP antibody, rat mo Anti-AKT phospho specific Apoptosis (Human) Antibod Cytokine (Mouse) Antibody Anti-ADAM-9 (A Disintigri Inflammation (Mouse) Anti Rabbit Anti-Nkx2.5 Cardia Human Dnak (HSP70) His ta MOUSE ANTI BORRELIA BURGD Atherosclerosis (Mouse) A Anti-ADAM-17 (A Disintegr Rabbit Anti-Nkx2.5 Cardia
#22509912 // Save this To Up
Model IgG monoclonal autoantibody-anti-idiotype pair for dissecting the humoral immune response to oxidized low density lipoprotein.Increasing evidence implicates IgG autoantibodies against oxidized forms of low density lipoprotein (oxLDL) in the pathophysiology of atherosclerotic arterial disease. However, insufficient knowledge of their structure and function is a key gap. Using an elderly LDL receptor-deficient atherosclerotic mouse, we isolated a novel IgG3k against oxLDL (designated MAb LO1). LO1 reacts with copper-oxidized LDL, but minimally with native LDL. Further analysis showed that MAb LO1 also reacts in vitro with malondialdehyde-conjugated LDL (MDA-LDL), a known key epitope in copper-oxidized LDL preparations. By screening a phage library expressing single chain variable region antibodies (scFv), we selected an anti-idiotype scFv (designated H3) that neutralizes MAb LO1 binding to MDA-LDL. Amino acid substitutions between H3 and an irrelevant control scFv C12 showed that residues in the H3 CDRH2, CDRH3, and CDRL2 are all critical for MAb LO1 binding, consistent with a conformational epitope on H3 involving both heavy and light chains. Comparison of amino acids in H3 CDRH2 and CDRL2 with apoB, the major LDL protein, showed homologous sequences, suggesting H3 has structural similarities to the MAb LO1 binding site on MDA-LDL. Immunocytochemical staining showed that MAb LO1 binds epitopes in mouse and human atherosclerotic lesions. The MAb LO1-H3 combination therefore provides a very promising model for analyzing the structure and function of an individual IgG autoantibody in relation to atherosclerosis.
2319 related Products with: Model IgG monoclonal autoantibody-anti-idiotype pair for dissecting the humoral immune response to oxidized low density lipoprotein.MOUSE ANTI APAAP COMPLEX, MOUSE ANTI HUMAN CD19 RPE MOUSE ANTI BOVINE ROTAVIR MOUSE ANTI CANINE DISTEMP anti-Superoxide Dismutase anti-Complement C3 (10A1) anti Adenovirus C11 IgG2a anti-Bid (8D2), Mouse mon anti-Peroxiredoxin Ⅲ (2 anti-α2-macroglobulin (3 anti HBcAg core IgG2a (mo anti-Casein kinase II β
#19836578 // Save this To Up
Antibodies conjugated with new highly luminescent Eu3+ and Tb3+ chelates as markers for time resolved immunoassays. Application to simultaneous determination of clenbuterol and free cortisol in horse urine.Highly luminescent Eu(3+) and Tb(3+) complexes of 10-[4-(3-isothiocyanatopropoxy)benzoylmethyl]-1,4,7,10-tetraazacyclododecane-1,4,7 triacetic acid Eu(3+) is a subset of 1 and Tb(3+) is a subset of 1 were conjugated with a goat anti-rabbit IgG and a rabbit anti-mouse IgG, respectively, and applied as markers in a time resolved immunoassay for simultaneous quantitative determination of anabolic compounds clenbuterol (CL) and hydrocortisone (HC). The assay was performed in horse urine, using a monoclonal antibody specific to CL and a rabbit polyclonal antibody specific to the free HC. These lanthanide chelates are very stable and highly luminescent in aqueous solution and allowed to reach 10 microg L(-1) and 40 microg L(-1) sensitivities for CL and for HC, respectively. Application to the horse urine, that is a very complex matrix, has a considerable interest in the control of illegal use of these compounds.
1251 related Products with: Antibodies conjugated with new highly luminescent Eu3+ and Tb3+ chelates as markers for time resolved immunoassays. Application to simultaneous determination of clenbuterol and free cortisol in horse urine.T-2 Toxin Mycotoxins ELIS Rabbit Anti-ZHX2 Alpha fe Cytokine (Mouse) Antibody Rabbit Anti-Human Androge Cell Cycle Phospho-Specif Inflammation (Human) Anti GPCR Signaling to MAPK ER NF-kB Phospho-Specific Ar Rabbit Anti-NPHS2 Podocin Angiogenesis (Human) Anti Rabbit Anti-APIP Apaf1 In Cytokine (Human) Antibody
#18829494 // Save this To Up
Therapy of advanced B-lymphoma xenografts with a combination of 90Y-anti-CD22 IgG (epratuzumab) and unlabeled anti-CD20 IgG (veltuzumab).Antibodies are effective therapeutic agents in cancer, but cures are rarely if ever obtained. Combination therapies are likely to be more effective than a single agent. In this study, the combination of a new unconjugated humanized anti-CD20 IgG, veltuzumab, with a (90)Y-conjugated humanized antibody to CD22 (epratuzumab) was evaluated for the treatment of B-cell lymphoma in a nude mouse model system.
1531 related Products with: Therapy of advanced B-lymphoma xenografts with a combination of 90Y-anti-CD22 IgG (epratuzumab) and unlabeled anti-CD20 IgG (veltuzumab).Mouse anti Canine IgE ant anti-Superoxide Dismutase anti Adenovirus E11 IgG2a anti-α2-macroglobulin (9 Mouse anti-bovine type II Mouse Anti Retinal Santig Mouse Anti-IgG1 neg. cont anti-Erk1 (33A5), Mouse m Mouse Anti-PP2A B PR55 al Mouse anti Human IgG anti MOUSE ANTI BOVINE ROTAVIR anti-GST tag (LF4G2), Mou
#18523585 // Save this To Up
Liposomal co-entrapment of CD40mAb induces enhanced IgG responses against bacterial polysaccharide and protein.Antibody against CD40 is effective in enhancing immune responses to vaccines when chemically conjugated to the vaccine antigen. Unfortunately the requirement for chemical conjugation presents some difficulties in vaccine production and quality control which are compounded when multivalent vaccines are required. We explore here an alternative to chemical conjugation, involving the co-encapsulation of CD40 antibody and antigens in liposomal vehicles.
1376 related Products with: Liposomal co-entrapment of CD40mAb induces enhanced IgG responses against bacterial polysaccharide and protein.anti HIV 1 p17 IgG1 (mono anti-pRb (retinoblastoma anti FAS IgG1 (monoclonal EZLys(TM) Bacterial Prote Mouse Anti-Histone H4 Me3 anti-Protein Tyrosine pho anti HIV 2 gp36 IgG1 (mon anti HCV core IgG2a (mono Rabbit Anti-Rat Androgen Enhanced Green Fluorescen anti HIV 1 p55 17 IgG1 (m Mouse Anti-Histone H4 Me1
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 Fax 0032 16 50 90 45
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50 Fax 01 43 25 01 60
52062 Aachen Deutschland
Tel 0241 40 08 90 86 Fax 0241 55 91 05 36
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
Schweiz Züri +41435006251
Česká republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Ελλάς Αθήνα +302111768494
Magyarország Budapest +3619980547
GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
GENTAUR Nederland BV
5521 DG Eersel Nederland
Tel 0208-080893 Fax 0497-517897
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93 Fax 02 36 00 65 94
53 Iskar Str. 1191 Kokalyane, Sofia