Search results for: Mouse Anti-GAPDH(3E12)-Loading Control Monoclonal Antibody, PE-Cy5.5 conjugated Isotype: IgG
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Immune suppression of food allergy by maternal IgG in murine models.Most of the patients develop food allergy early in life. The factors related to parental immune condition might be one of the conceivable causes.
Mouse Anti Salmonella typ anti HCMV IE pp65 IgG1 (m Mouse AntiInfluenza A Tar Hamster AntiSerine Protea Mouse AntiMRSA Target Ant Mouse Anti-Insulin-Like G anti-C1 inhibitor (4G12), HIV1 integrase antibody, DMPO, N1664A Host Mouse S Mouse AntiSerratia marces anti HSV (II) gB IgG1 (mo Mouse Anti P.aeruginosa s
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Internalization of targeted quantum dots by brain capillary endothelial cells in vivo.Receptors located on brain capillary endothelial cells forming the blood-brain barrier are the target of most brain drug delivery approaches. Yet, direct subcellular evidence of vectorized transport of nanoformulations into the brain is lacking. To resolve this question, quantum dots were conjugated to monoclonal antibodies (Ri7) targeting the murine transferrin receptor. Specific transferrin receptor-mediated endocytosis of Ri7-quantum dots was first confirmed in N2A and bEnd5 cells. After intravenous injection in mice, Ri7-quantum dots exhibited a fourfold higher volume of distribution in brain tissues, compared to controls. Immunofluorescence analysis showed that Ri7-quantum dots were sequestered throughout the cerebral vasculature 30 min, 1 h, and 4 h post injection, with a decline of signal intensity after 24 h. Transmission electron microscopic studies confirmed that Ri7-quantum dots were massively internalized by brain capillary endothelial cells, averaging 37 ± 4 Ri7-quantum dots/cell 1 h after injection. Most quantum dots within brain capillary endothelial cells were observed in small vesicles (58%), with a smaller proportion detected in tubular structures or in multivesicular bodies. Parenchymal penetration of Ri7-quantum dots was extremely low and comparable to control IgG. Our results show that systemically administered Ri7-quantum dots complexes undergo extensive endocytosis by brain capillary endothelial cells and open the door for novel therapeutic approaches based on brain endothelial cell drug delivery.
1696 related Products with: Internalization of targeted quantum dots by brain capillary endothelial cells in vivo.Human Internal Mammary Ar GFP Expressing Human Brai Human Large Intestine Mic GFP Expressing Mouse Brai Human Brain Microvascular GFP Expressing Human Inte Human Small Intestine Mic MarkerGeneTM in vivo lacZ Mouse Brain Microvascular Brain tumor tissue array Human Uterine Microvascul Brain-Specific Angiogenes
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Small-Animal PET Imaging of Pancreatic Cancer Xenografts Using a 64Cu-Labeled Monoclonal Antibody, MAb159.Overexpression of the GRP78 receptor on cell surfaces has been linked with tumor growth, metastasis, and resistance to therapy. We developed a (64)Cu-labeled probe for PET imaging of tumor GRP78 expression based on a novel anti-GRP78 monoclonal antibody, MAb159.
2337 related Products with: Small-Animal PET Imaging of Pancreatic Cancer Xenografts Using a 64Cu-Labeled Monoclonal Antibody, MAb159.Anti-Infectious Pancreati Anti-Infectious Pancreati Monoclonal antibody to hu Anti-Infectious Pancreati Monoclonal Anti-Breast Ca Anti-Infectious Pancreati Proteins and Antibodies H Monoclonal antibody to hu Proteins and Antibodies H Monoclonal Anti-Breast Ca HBsAg antibody, Monoclona Anti Human AGO3, Monoclon
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Real-time and sensitive detection of Salmonella Typhimurium using an automated quartz crystal microbalance (QCM) instrument with nanoparticles amplification.The accidental contamination of Salmonella in raw and processed foods is a major problem for the food industry worldwide. At present many of the currently used methods for Salmonella detection are time and labour intensive. Therefore, rapid detection is a key to the prevention and identification of problems related to health and safety. This paper describes the application of a new quartz crystal microbalance (QCM) instrument with a microfluidic system for the rapid and real time detection of Salmonella Typhimurim. The QCMA-1 bare gold sensor chip which contain two sensing array was modified by covalently immobilising the monoclonal capture antibody on the active spot and a mouse IgG antibody on the control spot using a conventional amine coupling chemistry (EDC-NHS). The binding of the Salmonella cells onto the immobilised anti-Salmonella antibody alters the sensor frequency which was correlated to cells concentration in the buffer samples. Salmonella cells were detected using direct, sandwich, and sandwich assay with antibody conjugated gold-nanoparticles. The performance of the QCM immunosensor developed with gold-nanoparticles gave the highest sensitivity with a limit of detection (LOD) ~10-20 colony forming unit (CFU) ml(-1) compared to direct and sandwich assay (1.83 × 10(2) CFU ml(-1) and 1.01 × 10(2) CFU ml(-1), respectively). The sensor showed good sensitivity and selectivity for Salmonella in the presence of other bacteria in real food samples and helped in reducing the pre-enrichment step, hence, demonstrating the potential of this technology for the rapid and sensitive microbial analysis.
2844 related Products with: Real-time and sensitive detection of Salmonella Typhimurium using an automated quartz crystal microbalance (QCM) instrument with nanoparticles amplification.Rabbit Anti-Salmonella ty Rabbit Anti-Salmonella ty Salmonella Real Time PCR Rabbit Anti-Salmonella ty Rabbit Anti-Salmonella ty Rabbit Anti-Salmonella ty Syringe pump can be contr Rabbit Anti-Salmonella ty Mouse Anti-Salmonella typ Rabbit Anti-Salmonella ty Rabbit Anti-Salmonella ty Salmonella Real Time PCR
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Identification of the optimal therapeutic antibody for fluorescent imaging of cutaneous squamous cell carcinoma.Intraoperative, real-time fluorescence imaging may significantly improve tumor visualization and resection and postoperatively, in pathological assessment. To this end, we sought to determine the optimal FDA approved therapeutic monoclonal antibody for optical imaging of human cutaneous squamous cell carcinoma (cSCC). A near-infrared (NIR) fluorescent probe (IRDye800) was covalently linked to bevacizumab, panitumumab or tocilizumab and injected systemically into immunodeficient mice bearing either cutaneous tumor cell lines (SCC13) or cutaneous human tumor explants. Tumors were then imaged and resected under fluorescent guidance with the SPY, an FDA-approved intraoperative imaging system, and the Pearl Impulse small animal imaging system. All fluorescently labeled antibodies delineated normal tissue from tumor in SCC13 xenografts based on tumor-to-background (TBR) ratios. The conjugated antibodies produced TBRs of 1.2-2 using SPY and 1.6-3.6 using Pearl; in comparison, isotype control antibody IgG-IRDye produced TBRs of 1.0 (SPY) and 0.98 (Pearl). Comparison between antibodies revealed them to be roughly equivalent for imaging purposes with both the SPY and Pearl (p = 0.89 SPY, p = 0.99 Pearl; one way ANOVA). Human tumor explants were also imaged and tumor detection was highest with panitumumab-IRDye800 when using the SPY (TBR 3.0) and Pearl (TBR 4.0). These data suggest that FDA approved antibodies may be clinically used for intraoperative detection of cSCC.
2654 related Products with: Identification of the optimal therapeutic antibody for fluorescent imaging of cutaneous squamous cell carcinoma.Multiple head and neck sq Esophageal squamous cell FDA Standard Frozen Tissu MarkerGeneTM Fluorescent Skin squamous cell carcin Multiple organ squamous c Non small cell lung carci Esophagus squamous cell c Normal rat multiple organ Lymph node metastatic squ Cervix squamous cell carc Non small cell lung carci
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Synthesis of site-specific antibody-drug conjugates using unnatural amino acids.Antibody-drug conjugates (ADCs) allow selective targeting of cytotoxic drugs to cancer cells presenting tumor-associated surface markers, thereby minimizing systemic toxicity. Traditionally, the drug is conjugated nonselectively to cysteine or lysine residues in the antibody. However, these strategies often lead to heterogeneous products, which make optimization of the biological, physical, and pharmacological properties of an ADC challenging. Here we demonstrate the use of genetically encoded unnatural amino acids with orthogonal chemical reactivity to synthesize homogeneous ADCs with precise control of conjugation site and stoichiometry. p-Acetylphenylalanine was site-specifically incorporated into an anti-Her2 antibody Fab fragment and full-length IgG in Escherichia coli and mammalian cells, respectively. The mutant protein was selectively and efficiently conjugated to an auristatin derivative through a stable oxime linkage. The resulting conjugates demonstrated excellent pharmacokinetics, potent in vitro cytotoxic activity against Her2(+) cancer cells, and complete tumor regression in rodent xenograft treatment models. The synthesis and characterization of homogeneous ADCs with medicinal chemistry-like control over macromolecular structure should facilitate the optimization of ADCs for a host of therapeutic uses.
1571 related Products with: Synthesis of site-specific antibody-drug conjugates using unnatural amino acids.anti GFP antibody, rat mo MMP (Human) Antibody Arra Anti-ACE-1 (Angiotension Rabbit Polyclonal Antibod Anti-HBsAg (Pre-S2 Specif Apoptosis (Human) Antibod Cytokine (Mouse) Antibody Anti-ADAM-10 (A Disintigr Inflammation (Mouse) Anti Atherosclerosis (Mouse) A Anti- ADAM-19 (A Disintig Cytokine (Human) Antibody
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Model IgG monoclonal autoantibody-anti-idiotype pair for dissecting the humoral immune response to oxidized low density lipoprotein.Increasing evidence implicates IgG autoantibodies against oxidized forms of low density lipoprotein (oxLDL) in the pathophysiology of atherosclerotic arterial disease. However, insufficient knowledge of their structure and function is a key gap. Using an elderly LDL receptor-deficient atherosclerotic mouse, we isolated a novel IgG3k against oxLDL (designated MAb LO1). LO1 reacts with copper-oxidized LDL, but minimally with native LDL. Further analysis showed that MAb LO1 also reacts in vitro with malondialdehyde-conjugated LDL (MDA-LDL), a known key epitope in copper-oxidized LDL preparations. By screening a phage library expressing single chain variable region antibodies (scFv), we selected an anti-idiotype scFv (designated H3) that neutralizes MAb LO1 binding to MDA-LDL. Amino acid substitutions between H3 and an irrelevant control scFv C12 showed that residues in the H3 CDRH2, CDRH3, and CDRL2 are all critical for MAb LO1 binding, consistent with a conformational epitope on H3 involving both heavy and light chains. Comparison of amino acids in H3 CDRH2 and CDRL2 with apoB, the major LDL protein, showed homologous sequences, suggesting H3 has structural similarities to the MAb LO1 binding site on MDA-LDL. Immunocytochemical staining showed that MAb LO1 binds epitopes in mouse and human atherosclerotic lesions. The MAb LO1-H3 combination therefore provides a very promising model for analyzing the structure and function of an individual IgG autoantibody in relation to atherosclerosis.
2359 related Products with: Model IgG monoclonal autoantibody-anti-idiotype pair for dissecting the humoral immune response to oxidized low density lipoprotein.MOUSE ANTI CANINE DISTEMP MOUSE ANTI BOVINE ROTAVIR MOUSE ANTI APAAP COMPLEX, MOUSE ANTI HUMAN CD19 RPE anti-Superoxide Dismutase anti-CDK5 (8A1), Mouse mo anti-ZAP70 (49B4), Mouse anti HBcAg core IgG2a (mo Rabbit Anti-HDL high dens anti-GAPDH (8C2), Mouse m anti-GFP (18A11), Mouse m anti-Glutathione Peroxida
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Antibodies conjugated with new highly luminescent Eu3+ and Tb3+ chelates as markers for time resolved immunoassays. Application to simultaneous determination of clenbuterol and free cortisol in horse urine.Highly luminescent Eu(3+) and Tb(3+) complexes of 10-[4-(3-isothiocyanatopropoxy)benzoylmethyl]-1,4,7,10-tetraazacyclododecane-1,4,7 triacetic acid Eu(3+) is a subset of 1 and Tb(3+) is a subset of 1 were conjugated with a goat anti-rabbit IgG and a rabbit anti-mouse IgG, respectively, and applied as markers in a time resolved immunoassay for simultaneous quantitative determination of anabolic compounds clenbuterol (CL) and hydrocortisone (HC). The assay was performed in horse urine, using a monoclonal antibody specific to CL and a rabbit polyclonal antibody specific to the free HC. These lanthanide chelates are very stable and highly luminescent in aqueous solution and allowed to reach 10 microg L(-1) and 40 microg L(-1) sensitivities for CL and for HC, respectively. Application to the horse urine, that is a very complex matrix, has a considerable interest in the control of illegal use of these compounds.
2268 related Products with: Antibodies conjugated with new highly luminescent Eu3+ and Tb3+ chelates as markers for time resolved immunoassays. Application to simultaneous determination of clenbuterol and free cortisol in horse urine.T-2 Toxin Mycotoxins ELIS Rabbit Anti-Cullin 5 CUL5 T-Cell Receptor Signaling Cytokine (Human) Antibody Cytokine (Mouse) Antibody Syringe pump can be contr Goat Anti-Human Androgen EnzyChrom™ Kinase Assay Rabbit Anti-TNIP2 ABIN2 T Th1 Th2 Th17 (Human) Anti Cytokine (Human) Antibody Apoptosis antibody array
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Therapy of advanced B-lymphoma xenografts with a combination of 90Y-anti-CD22 IgG (epratuzumab) and unlabeled anti-CD20 IgG (veltuzumab).Antibodies are effective therapeutic agents in cancer, but cures are rarely if ever obtained. Combination therapies are likely to be more effective than a single agent. In this study, the combination of a new unconjugated humanized anti-CD20 IgG, veltuzumab, with a (90)Y-conjugated humanized antibody to CD22 (epratuzumab) was evaluated for the treatment of B-cell lymphoma in a nude mouse model system.
2201 related Products with: Therapy of advanced B-lymphoma xenografts with a combination of 90Y-anti-CD22 IgG (epratuzumab) and unlabeled anti-CD20 IgG (veltuzumab).anti-Thioredoxin Reductas anti-Apolipoprotein A-I ( anti HBcAg core IgG2b (mo Mouse anti-porcine type I Sheep Anti-IgG2 Antibodie anti-GAPDH (7B), Mouse mo anti-α2-macroglobulin (2 anti-Peroxiredoxin I (3G5 Mouse anti human type II Sheep Anti-Human IgG1 Ant Anti monomethyl Histone H anti-Protein Tyrosine pho
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Liposomal co-entrapment of CD40mAb induces enhanced IgG responses against bacterial polysaccharide and protein.Antibody against CD40 is effective in enhancing immune responses to vaccines when chemically conjugated to the vaccine antigen. Unfortunately the requirement for chemical conjugation presents some difficulties in vaccine production and quality control which are compounded when multivalent vaccines are required. We explore here an alternative to chemical conjugation, involving the co-encapsulation of CD40 antibody and antigens in liposomal vehicles.
1504 related Products with: Liposomal co-entrapment of CD40mAb induces enhanced IgG responses against bacterial polysaccharide and protein.EZLys(TM) Bacterial Prote anti HIV 2 gp36 IgG1 (mon anti FAS IgG1 (monoclonal Mouse Anti-Histone H4 Me3 anti-Protein Tyrosine pho Rabbit Anti-Rat Androgen Enhanced Green Fluorescen anti HCV core IgG2a (mono anti FAS IgG1 (monoclonal anti HIV 1 p55 17 IgG1 (m anti-pRb (retinoblastoma Mouse Anti-Histone H4 Me1
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