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Search results for: Mouse Anti-Human GFAP Antibodies

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#18353289   2008/03/04 To Up

Müller glia factors induce survival and neuritogenesis of peripheral and central neurons.

We have examined the trophic effects of conditioned media obtained from purified murine Müller glia cells on chick purified sympathetic or dorsal root ganglia (DRG) neurons and on Retinal Ganglion Cells (RGC) from postnatal mice. Purified murine Müller glia cultures stained positively for vimentin, GFAP or S-100, but were negative for neuronal markers. Murine Müller glial conditioned medium (MMG) was concentrated and at 1:1 dilution supported 100% survival of chick or rat sympathetic neurons after 48 h compared to <5% in controls. Partial purification of the MMG using centriprep concentrators showed that trophic activity is from molecules above 10 kDa. MMG stimulated AKT, ERK and pStat3 in sympathetic neurons. Sympathetic or DRG neuronal survival induced by MMG was blocked by anti-human NGF, but not by anti-human CNTF (sympathetic) or by anti-BDNF (DRGs) neutralizing antibodies. MMG also induced neurite outgrowth in P4 mice retinal explants and on isolated RGC. RGCs plated on top of Müller glia cells had a much better survival rate (>80%, 96 h) compared to laminin+poly-L-lysine substrates. In conclusion, we show that purified mice Müller glia cultures secrete NGF that support peripheral neuronal survival and other unidentified trophic molecules that induce RGC survival and neuritogenesis.
Ricardo Augusto de Melo Reis, Mauricio e Castro Cabral-da-Silva, Fernando Garcia de Mello, Jeremy S H Taylor

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#10440241   // To Up

Differential expression of nitric oxide synthase in experimental uveoretinitis.

To investigate the site and the cellular source of inducible nitric oxide synthase (iNOS) expression in human S-antigen peptide-induced experimental autoimmune uveoretinitis (EAU).
J Zhang, L Y Wu, G S Wu, N A Rao

2575 related Products with: Differential expression of nitric oxide synthase in experimental uveoretinitis.

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#2840106   // To Up

[Interleukin 1 and gliomas: immunohistochemical and immunocytochemical examinations].

Interleukin 1 is a monokine produced by macrophage and has an ability to activate thymocytes. In addition to the immunological regulatory effect, interleukin 1 has attracted a great deal of investigators as a new peptide hormone that was secreted by many cells and has a various physiological activities. In central nervous systems, interleukin 1 promotes the glial cells proliferations on the injured brain and the fetal brain. The cell sources of interleukin 1 in central nervous systems are considered to the microglial cells. On gliomas, Lachmann and Dinarello reported growth promoting effect of IL-1 on U-373 MG human glioblastoma cell. The authors investigated the roles and effects of IL-1 on the growth of gliomas using recombinant human IL-1 beta and anti-HuIL-1 beta monoclonal antibody. On Immunohistochemistry, paraffin sections of 10 cases of gliomas were stained with immunoperoxidase method using anti-human IL-1 beta and anti-GFAP mouse monoclonal antibody. All astrocytomas examined and 2 of 4 glioblastomas were stained by anti-IL-1 beta. The origin of IL-1 that was stained by immunoperoxidase staining is unknown. The authors think it that IL-1 existed in glioma cells were secreted by microglial cells or that the glioma cells themselves secreted IL-1. In either case, IL-1 must be related to the growth of glioma in situ. On immunocytochemistry, U-373 MG human glioblastoma cells purchased from ATCC were incubated on cover-slip with 0 and 10 U/ml of rHuIL-1 beta for 3 or 7 days. The cells were stained with immunoperoxidase method using anti-GFAP monoclonal antibody.(ABSTRACT TRUNCATED AT 250 WORDS)
S Tanaka, H Kamitani, T Hori, Y Hirai, S Nakai, K Kawai

1143 related Products with: [Interleukin 1 and gliomas: immunohistochemical and immunocytochemical examinations].

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