Search results for: Mouse Anti-Human GH Antibodies
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#1299418 // To Up
Pattern of antigens A and B distribution on the surface of AB group red blood cells--an argument in support of the concept of "genetic oscillation".
Using a mixture of mouse monoclonal antibodies anti red blood cell (RBC) AgA and human antibodies anti RBC AgB coupled to fluorescein isothiocyanate (FITC) conjugated goat antimouse monoclonal antibodies and to phycoerythrin (PE) conjugated goat antihuman monoclonal antibodies, we obtained the distribution of these antigens on the AB RBC surface. The analysis of 30 samples of 30,000-100,000 RBCs each was carried out using flow cytometry and revealed three kinds of AB RBC populations: (1) a population characterized by maximum concentration of AgA and minimum concentration of AgB; (2) a complementary population characterized by minimum concentration of AgA and maximum concentration of AgB; (3) an intermediary equilibrated population with about equal concentrations of the A and B antigens. This pattern corresponds to the pattern of oscillating genetic activity defined as "genetic oscillation", a concept amply discussed in the paper.M Balea, S Purice, B Chatelain, C Chatelain, Y Cornet, A Dromelet, M Garrino, S Balea
1353 related Products with: Pattern of antigens A and B distribution on the surface of AB group red blood cells--an argument in support of the concept of "genetic oscillation".
1100 100ug/vial100 Tests0.2 mg100.00 ug 100ulRelated Pathways
#2510809 // To Up
Immunoreactivity of hormonally-characterized human endocrine cells against three novel anti-human chromogranin B(B11 and B13) and chromogranin A (A11) monoclonal antibodies.
Two novel monoclonal antibodies, called B11 and B13, directed exclusively against human chromogranin B (CgB) and another antibody, A11, specific for human chromogranin A (CgA), were obtained by immunization of mice with chromaffin granules, the fusion of their splenocytes, the screening of hybridomas supernatants by ELISA and immunohistochemistry, and characterization of the antibodies by two-dimensional immunoblotting. The antibodies were used in immunohistochemical tests to investigate the distribution of CgA and CgB in hormonally-identified cells of the human endocrine system. The A11 antibody confirmed the occurrence of CgA in gut EC, ECL, gastrin, secretin and neurotensin cells, pancreatic A and PP cells, parathyroid chief cells, pituitary TSH and gonadotrope cells and adrenal medullary cells. Only a fraction of CgA-immunoreactive cells in the human gut and pancreas showed C-terminus arginine-glycinamide immunoreactivity, suggesting pancreastatin storage. Both CgB antibodies showed immunoreactivity in gastrin cells, intestinal (but not gastric) EC cells, pancreatic A and PP cells, pituitary TSH and gonadotrope cells and adrenal medullary cells. In addition, the B11 antibody stained thyroid C cells and the B13 antibody stained the Golgi area of pituitary GH cells. It is concluded that most CgB is stored in the same cells showing CgA, although some CgA-rich cells, like gastric EC and ECL cells. lacked B11 and B13 immunoreactivities and some CgA-poor cells, like human thyroid C cells, showed intense B11 immunostaining.R Buffa, A Gini, M Pelagi, A G Siccardi, C Bisiani, A Zanini, E Solcia
1199 related Products with: Immunoreactivity of hormonally-characterized human endocrine cells against three novel anti-human chromogranin B(B11 and B13) and chromogranin A (A11) monoclonal antibodies.
1 ml0.1 mg0.1 mg1 ml1 ml0.1 ml100 μg200 100.00 ug50ul25 TESTS0.5 mlRelated Pathways
#7094888 // To Up
The same region of human growth hormone is involved in its binding to various receptors.
The Fab fragments of three monoclonal antihuman GH (anti-hGH) antibodies, among five tested, inhibited the binding of the hormone to the receptors of the human lymphoid cell line IM-9 and liver membranes of the pregnant rabbit. The results were similar for the receptors of human lymphocytes and rabbit liver, suggesting that both receptors reacted with the same region of the hormone. The Fab fragments of the most inhibitory antibody also inhibited the down-regulation by the hormone of the receptors on human lymphocytes. The fragments of this antibody completely blocked the binding of the hormone to the receptors of the rabbit liver, despite the fact that this carries two or more classes of receptors. Therefore, all of these various receptors apparently interact with the same region of the hormone. Three synthetic peptides extending from residues 19-128, 73-128, and 98-128 failed to inhibit the binding of hGH to its lymphocyte or liver receptors however, these peptides reacted significantly with the monoclonal antibody, which was the strongest inhibitor of the interaction of hGH with the cellular receptor, confirming that the receptor-binding site of the hormone is in the amino-terminal part of the molecule and suggesting indirectly that the short sequences 98-128 participates in the constitution of the receptor-binding site of the hormone.L A Retegui, P De Meyts, C Peña, P L Masson
1281 related Products with: The same region of human growth hormone is involved in its binding to various receptors.
100 μg100 μg1 mg100 μg100 μg100 μg100 μg100 μg100 μg100 μg1 mg100 μgRelated Pathways
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