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Search results for: Mouse Anti-Human HLA ABC Antibodies

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Participation of stem cells from human cord blood in skeletal muscle regeneration of SCID mice.

In this report, we demonstrate the participation of human cord blood (HUCB) stem cells in the skeletal muscle regeneration of SCID (severe combined immunodeficient) mice.
Edyta Brzóska, Iwona Grabowska, Grazyna Hoser, Władysława Stremińska, Danuta Wasilewska, Eugeniusz Krzysztof Machaj, Zygmunt Pojda, Jerzy Moraczewski, Jerzy Kawiak

2178 related Products with: Participation of stem cells from human cord blood in skeletal muscle regeneration of SCID mice.

1.00 flask1 mg10 ug1.00 flask1 Unit1100 extractions50 mg1ml1.00 flask1.00 flask

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[The HLA-I and HLA-II expression evaluation in liver biopsy specimens of patients with chronic viral hepatitis].

The main an etiological agents of chronic hepatitis are viral infections. The viral infection course and outcome depend mostly on the immunological response. Infected hepatocytes are damaged by appropriately viral antigen-specific cytolytic T lymphocytes. Those sensitised T cells react only with those hepatocytes which express viral antigen and antigen HLA on membrane surface. The aim of this study was to evaluate the expression of selected histocompatibility antigens HLA in liver biopsy specimens of patients with chronic viral hepatitis. Seventeen patients with chronic persistent hepatitis (inflammatory activity 1-4 points according to Scheuer scale modified by Gabriel) and 27 patients with chronic active hepatitis (5-10 points) were studied. In these groups of patients the intensity of HLA-I (A, B, C), HLA-II (DR) expression in liver biopsy specimens, alanine aminotransferase activity, markers of HBV and HCV in serum were examined. The monoclonal mouse anti-human antibodies and streptavidin-biotin with alkaline phosphatase method for estimation of HLA-I, HLA-II was used. Results were statistically analysed using Mann-Whitney's U test and Spearman's rank correlation test. Generally, the expression of HLA-I and HLA-II on hepatocyte membrane was shown. Significant differences in expression of HLA-II among studied groups were observed, moreover the highest degree of HLA-II intensity in the group of patients with greater inflammation activity was significantly more frequently observed. The expression of HLA-I, HLA-II was regardless of the viral a etiology and serological markers of HBV replication. The degree of studied parameters expression was positively correlated with biochemical activity of inflammation.
W Stolarz, A Wiczkowski, R Wojnicz, A Dziambor, L Kepa, M Biskupska-Karasińska

1821 related Products with: [The HLA-I and HLA-II expression evaluation in liver biopsy specimens of patients with chronic viral hepatitis].

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Secretion of genetically engineered human/mouse class I antigens.

Two soluble, secreted forms of HLA-B7 were engineered by the creation of hybrid human/mouse molecules containing the polymorphic 5' region of the HLA-B7 gene and the secretory 3' region of the mouse Q10d gene. The hybrid, designated F1, is the first construct with only human extracytoplasmic domains, consisting of exons for the leader peptide and the three extracellular domains (alpha 1, alpha 2, alpha 3) of B7 spliced to the exons for the Q10d truncated transmembrane and 3' untranslated (3'UT) sequences. The second construct, designated C2, is similar but has the human alpha 3 replaced by the Q10 alpha 3 domain. Protein product from each construct was best demonstrated after gene transfection into the J27.2 cell line. In particular, secretion of the F1 product proves that the Q10 alpha 3 domain is not necessary for secretion of class I/Q10 hybrids. Moreover, the two soluble B7 forms, which differ only in their alpha 3 domain, are similarly recognized by monoclonal antibodies W6/32 (anti-HLA-ABC), BBM.1 (anti-human beta 2 microglobulin), and allo-B7-antibody, but differentially recognized by monoclonal antibody Q1/28 (anti-HLA class I heavy chain). Production of such soluble hybrid class I molecules in large amounts should allow critical structural and functional studies of these proteins.
N Cohen, J S Crawford, D D Hiraki, F C Grumet

2555 related Products with: Secretion of genetically engineered human/mouse class I antigens.

100.00 ug530 μg100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100 μg100ug Lyophilized100ug Lyophilized

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Correlation of vascular permeability and blood flow with monoclonal antibody uptake by human Clouser and renal cell xenografts.

The specific uptake of 125I-A6H antibody by xenografts of the human renal cell carcinoma (RCC) TK177G in the athymic mouse was considerably greater than that seen for other human tumor xenografts and their associated antibodies (e.g., 125I-B6.2 uptake by the human breast carcinoma, Clouser). In addition the A6H-RCC model also demonstrated both greater localization indices and absolute amount of antibody bound than did the B6.2-Clouser model. Several physiological factors were studied to assess whether they might play a role in this greater specific uptake. Vascular volume was determined using the in situ labeling of red blood cells with 99mTc. Vascular permeability was determined by measuring the amount of 125I-labeled bovine serum albumin and 131I-labeled nonspecific IgG1 (anti-horseradish peroxidase) extravasated out of the tumor vasculature during 1 hr. Relative blood flow to the tumor was determined using the 86Rb method. Blood flow and vascular permeability were found to be significantly greater in the RCC tumor xenografts than in Clouser tumors. Differences in vascular permeability were especially dramatic, showing the vasculature of the RCC xenograft was twice as permeable as that of the Clouser tumor. Animals bearing either RCC or Clouser xenografts were injected with a monoclonal antibody to human major histocompatibility complexes (125I-labeled anti-human histocompatibility complex A, B, C). Tumor uptake of 125I-labeled anti-human histocompatibility complex A, B, C was found to be 5 times greater in RCC than Clouser xenografts. These results, therefore, suggest that the differences seen in the physiological factors studied can account for some of the greater specific 125I-A6H uptake by the RCC tumor than 125I-B6.2 uptake by the Clouser xenograft.
H Sands, P L Jones, S A Shah, D Palme, R L Vessella, B M Gallagher

2029 related Products with: Correlation of vascular permeability and blood flow with monoclonal antibody uptake by human Clouser and renal cell xenografts.

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Ontogeny of the MHC antigens on human trophoblast cells during the first trimester of pregnancy.

The presence and the ontogeny of class I and class II major histocompatibility (MHC) antigens were investigated on human trophoblast cells in single cell suspensions of collagenase dispersed placentae during the first trimester of pregnancy by using a highly sensitive radioautographic approach. Cells were treated with monoclonal anti-HLA-A,B,C or anti-HLA-DR (or anti-human Ia) antibodies followed by 125I-labeled protein A, normal mouse serum, or medium alone used as negative controls, and monoclonal antibodies against trophoblast specific antigenic markers (anti-Trop 2, NDOG-1, and NDOG-2) used as positive controls for specificity of trophoblast labeling. Tissue controls were provided by Ficoll-paque-separated adult human peripheral blood lymphocytes (PBL), and placental lymphocytes were used as additional internal controls. Results revealed variable but specific labeling of cytotrophoblast cells for class I but not class II MHC antigens at all gestational stages during the first trimester. The incidence of HLA-A,B,C antigen-bearing cytotrophoblast cells in early gestational (5 to 8 wk) placentae was high (75 to 91%) in the minority, but was low to medium (35 to 66%) in placentae at later gestational intervals (9 to 12 wk). The initial antigen density on the labeled cytotrophoblast cells as reflected by the median silver grain counts per unit area was approximately 75% of that noted on adult PBL or placental lymphocytes at 5 to 7 wk. The density then dropped to 50% of the initial level by the eighth week of gestation with no further change up to 12 wk. Syncytiotrophoblast cells revealed no appreciable labeling for either antigen class. This could not be explained by antigen stripping due to the collagenase dispersion procedure; specific labeling was not detectable on the syncytiotrophoblast cell layer in situ in histologic sections after intact chorionic villi had been directly exposed to 125I-labeled monoclonal anti-class I antibody. These results are qualitatively similar to our findings in the mouse in revealing the presence of class I MHC antigens on a major population of trophoblast cells.
B Montgomery, P K Lala

1556 related Products with: Ontogeny of the MHC antigens on human trophoblast cells during the first trimester of pregnancy.

1mg102 100ul 100ul1001 ml1mg1.00 flask 100ul1002000 IU

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