Search results for: Mouse Anti-Pig TNF-alpha Antibodies
#26777482 
2016/01/09
To Up
Beneficial effects of the transgenic expression of human sTNF-αR-Fc and HO-1 on pig-to-mouse islet xenograft survival.
Both human soluble tumor necrosis factor-α receptor-Fc (sTNF-αR-Fc) and heme oxygenase-1 (HO-1) transgenic pigs have been generated previously for xenotransplantation. Here, we investigated whether overexpression of sTNF-αR-Fc or HO-1 in pig islets prolongs islet xenograft survival. Adult porcine islets were isolated from human sTNF-αR-Fc or HO-1 transgenic and wild type pigs, and were transplanted into diabetic nude mice. Effects of the expression of both genes on islet apoptosis, chemokine expression, cellular infiltration, antibody production, and islet xenograft survival were analyzed. Human sTNF-αR-Fc transgenic pigs successfully expressed sTNF-αR-Fc in the islets; human HO-1 transgenic pigs expressed significant levels of HO-1 in the islets. Pig-to-mouse islet xenograft survival was significantly prolonged in both the sTNF-αR-Fc and HO-1 groups compared with that in the wild type group. Both the sTNF-αR-Fc and HO-1 groups exhibited suppressed intragraft expression of monocyte chemoattractant protein-1 (MCP-1) and decreased perigraft infiltration of immune cells. However, there was no difference in the anti-pig antibody levels between the groups. Apoptosis of islet cells during the early engraftment was suppressed only in the HO-1 group. Porcine islets from both sTNF-αR-Fc and HO-1 transgenic pigs prolonged xenograft survival by suppressing islet cell apoptosis or secondary inflammatory responses following islet death, indicating that these transgenic pigs might have applications in successful islet xenotransplantation.Ji-Jing Yan, Hye-Jeong Yeom, Jong Cheol Jeong, Jae-Ghi Lee, Eun Won Lee, Bumrae Cho, Han Sin Lee, Su Jin Kim, Jong-Ik Hwang, Sung Joo Kim, Byeong-Chun Lee, Curie Ahn, Jaeseok Yang
1266 related Products with: Beneficial effects of the transgenic expression of human sTNF-αR-Fc and HO-1 on pig-to-mouse islet xenograft survival.
100ug Lyophilized100ug100ug Lyophilized100ug Lyophilized100ul100ug Lyophilized100ug Lyophilized100ug Lyophilized100μl100ug Lyophilized100ug Lyophilized100ug Lyophilized
Related Pathways
#18447887 //
To Up
Characterization of porcine UL16-binding protein 1 endothelial cell surface expression.
Natural killer (NK) cells participate in the immune response against solid organ allo- and xenografts and are tightly regulated through signals mediated by inhibiting and activating receptors expressed on their cell surface. Human NK cytotoxicity against porcine endothelial cells (pEC) is mediated by the interaction of the activating human NK receptor hNKG2D and its corresponding ligand on pEC, porcine UL-16 binding protein 1 (pULBP1). The aim of the present study was to characterize the regulation of pULBP1 cell-surface expression on primary porcine aortic endothelial cells (PAEC).Benjamin G Lilienfeld, Anita Schildknecht, Lukas L Imbach, Nicolas J Mueller, Mårten K J Schneider, Jörg D Seebach