Search results for: Mouse Anti-beta-Amyloid(1-16) (human) Polyclonal Antibody, PE-Cy5.5 conjugated Isotype: IgG
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Carbohydrate Specificity and Isotypes of Monoclonal and Polyclonal Antibodies to Conjugated Tetrasaccharide, a Synthetic Analogue of Repeating Unit of Capsular Polysaccharide of Streptococcus Pneumoniae Serotype 14.We studied carbohydrate specificity and isotypes of antibodies to BSA-conjugated tetrasaccharide, a repeating unit of the capsular polysaccharide of Streptococcus pneumoniae serotype 14, in mouse polyclonal sera and hybridoma-synthesized products. Natural IgM antibodies to the tetrasaccharide containing epitopes similar to surface carbohydrate structures of mammalian and human cells in low titers were determined in native mouse serum by ELISA using biotinylated tetrasaccharide and synthetic capsular polysaccharide as the solid-phase antigens. Polyclonal sera to the conjugated tetrasaccharide contained IgM and all subclasses of IgG antibodies, which were detected in a higher titer when the biotinylated tetrasaccharide was used as a solid phase antigen compared to synthetic capsular polysaccharide. Monoclonal antibodies to S. pneumoniae serotype 14 tetrasaccharide were identified in an equivalent titer using either biotinylated tetrasaccharide or synthetic capsular polysaccharide. Monoclonal antibodies obtained in vitro belonged to IgM isotype and cross-reacted with secondary full-size IgG antibodies. In the serum of mice inoculated with hybridoma, IgM and IgG2a antibodies recognizing the tetrasaccharide epitope in the structure of synthetic capsular polysaccharide were simultaneously determined.
2774 related Products with: Carbohydrate Specificity and Isotypes of Monoclonal and Polyclonal Antibodies to Conjugated Tetrasaccharide, a Synthetic Analogue of Repeating Unit of Capsular Polysaccharide of Streptococcus Pneumoniae Serotype 14.Clostridum difficile toxi MOUSE ANTI HUMAN CD19 RPE Chlamydia pneumoniae anti Clostridum difficile toxi Rat monoclonal anti mouse RABBIT ANTI GSK3 BETA (pS Goat Anti-Human Androgen Mac3 antibody (FITC), Con MOUSE ANTI HUMAN CD15, Pr Androgen Receptor (Phosph Diphtheria toxin antibody Shiga Toxin 2 antibody, M
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Enhanced and long term immunogenicity of a Her-2/neu multi-epitope vaccine conjugated to the carrier CRM197 in conjunction with the adjuvant Montanide.We previously identified three short single peptides (P4, P6 and P7) representing different B-cell epitopes on the extracellular domain of Her-2/neu for a vaccine that was tested in a phase-I clinical trial. Here we describe the improvement of the multi peptide vaccine by fusing the single peptides to a hybrid peptide P467.
2087 related Products with: Enhanced and long term immunogenicity of a Her-2/neu multi-epitope vaccine conjugated to the carrier CRM197 in conjunction with the adjuvant Montanide.FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Multiple organ tumor tiss Thermal Shaker with cooli Goat Anti- Neudesin NENF, FDA Standard Frozen Tissu FDA Standard Frozen Tissu Rabbit Anti-Integrin alph Rabbit Anti-IAA (Indole-3 Rabbit Anti-Phospho-INPPL
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Indirect Back-Titration ELISA: A New Format for Estimation of Human Tissue Kallikreins.Enzyme-linked immunosorbent assay (ELISA) is either based on sandwich, competitive, or inhibition type of format. However, these formats need 2 or 3 monoclonal antibodies (moAB) to estimate 1 antigen. To get a cost-effective, high throughput, ELISA for estimation of human tissue kallikreins we have now developed an indirect, back-titration style, Time Resolved ImmunoFluorometric (TRIF) ELISA that uses only 1 antigen-specific moAB and a general polyclonal antibody. Polystyrene microtiter plate wells coated with a capture antibody, a mouse moAB prepared against a specific human tissue kallikrein are allowed to interact either with the corresponding pure antigen, as the calibrator, or with the corresponding antigen present in a biological fluid or tissue extract. The detection antibody, anti-mouse IgG conjugated with alkaline phosphatase, is added to find the antigen-free immobilized capture moAB. Conjugated enzyme is allowed to hydrolyze diflunisal phosphate to produce a highly fluorescent complex. The fluorescence measured in TRIF mode corresponds to the antigen-free immobilized capture moAB and is used to quantify antigen-bound capture moAB. The detection antibody binds with the antigen-free capture moAB and strength of the signal correlates inversely with the amount of antigen bound to the capture moAB. With a minimum detection level of 20 ng/L the assay has no cross-reactivity with several test molecules. The method is sensitive, specific, applicable to a variety of biological samples, and cost-effective as it uses only 1 moAB and a polyclonal antibody. Using this assay, a single epitope can be estimated without purification.
1290 related Products with: Indirect Back-Titration ELISA: A New Format for Estimation of Human Tissue Kallikreins.MOUSE ANTI HUMAN CD15, Pr NATIVE HUMAN PROLACTIN, P MOUSE ANTI HUMAN CD19 RPE MOUSE ANTI HUMAN CD15, Pr ELISA kit CLGI,Collagenas 10x ELISA WASH BUFFER, Pr NATIVE HUMAN PROLACTIN, P Leptin ELISA Kit, Human A Human Apolipoprotein AI E Monkey Apolipoprotein CII Human interleukin 2(IL-2) Human borrelia burgdorfer
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Emergent properties of nanosensor arrays: applications for monitoring IgG affinity distributions, weakly affined hypermannosylation, and colony selection for biomanufacturing.It is widely recognized that an array of addressable sensors can be multiplexed for the label-free detection of a library of analytes. However, such arrays have useful properties that emerge from the ensemble, even when monofunctionalized. As examples, we show that an array of nanosensors can estimate the mean and variance of the observed dissociation constant (KD), using three different examples of binding IgG with Protein A as the recognition site, including polyclonal human IgG (KD μ = 19 μM, σ(2) = 1000 mM(2)), murine IgG (KD μ = 4.3 nM, σ(2) = 3 μM(2)), and human IgG from CHO cells (KD μ = 2.5 nM, σ(2) = 0.01 μM(2)). Second, we show that an array of nanosensors can uniquely monitor weakly affined analyte interactions via the increased number of observed interactions. One application involves monitoring the metabolically induced hypermannosylation of human IgG from CHO using PSA-lectin conjugated sensor arrays where temporal glycosylation patterns are measured and compared. Finally, the array of sensors can also spatially map the local production of an analyte from cellular biosynthesis. As an example, we rank productivity of IgG-producing HEK colonies cultured directly on the array of nanosensors itself.
2945 related Products with: Emergent properties of nanosensor arrays: applications for monitoring IgG affinity distributions, weakly affined hypermannosylation, and colony selection for biomanufacturing.MOUSE ANTI APAAP COMPLEX, MOUSE ANTI BOVINE ROTAVIR MOUSE ANTI CANINE DISTEMP MOUSE ANTI HUMAN CD19 RPE MOUSE ANTI HUMAN CD15, Pr PERMANENT AQUEOUS MOUNTIN NATIVE HUMAN PROLACTIN, P RABBIT ANTI GSK3 BETA (pS 10X PHOSPHATE BUFFERED SA MarkerGene™ LysoLive™ NATIVE HUMAN PROLACTIN, P 10x ELISA WASH BUFFER, Pr
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Removal of therapeutic anti-lymphocyte antibodies from human sera prior to anti-human leukocyte antibody testing.Both monoclonal (e.g. Orthoclone (OKT3), rituximab) and polyclonal (e.g. ATGAM, Thymoglobulin (Thymo)) anti-lymphocyte Abs (ALAs) are used extensively in organ transplantation for immunosuppression induction, desensitization, and treatment of acute rejection. ALAs often interfere with post transplant immunologic monitoring. We describe a method that uses magnetic beads to selectively remove ALAs from patient serum. Rabbit anti-mouse Fc-specific (180 mug), or rabbit anti-mouse Fab-specific (180 microg), or rabbit anti-horse heavy and light chain-specific and rabbit anti-horse F(ab')2 (200 microg) (Jackson Immunoresearch) was adsorbed to 6.7 x 10(8) Dynabeads M-280 conjugated with sheep anti-rabbit IgG (Dynal Biotech). Fifty microliters of normal human serum (NHS) with 2 microg/ml of OKT3 or 100 microg/ml ATGAM, Thymo, or rituximab were incubated with conjugated beads for several incubations. NHS containing ALAs before and after treatment by the protocol were incubated with human lymphocytes and labeled with FITC-antibody to immunoglobulin of the species used to produce the particular ALA. Residual ALA was determined using flow cytometry. Average median channel for serum with or without ALA was 11.1 and 0.120, respectively for OKT3; 64.4 and 0.344 for ATGAM; 108.5 and 0.200 for Thymo; and 1022.5 and 11.4 for rituximab. Treatment lowered the median channel for serum with OKT3 to 0.103, 0.309 for ATGAM, 0.199 for Thymo, and 12.1 for rituximab. ALAs can be effectively removed from serum by the use of magnetic beads conjugated with Ab specific for ALA thereby permitting immunologic monitoring without interference.
2013 related Products with: Removal of therapeutic anti-lymphocyte antibodies from human sera prior to anti-human leukocyte antibody testing.Rabbit Anti-Human TopBP1 Mouse anti Human IgE anti Goat Anti-Human TOM1L1 SR Mouse Anti-Human CD49d Ly Mouse Anti-Human CD103 (I Rabbit Anti-Human Toll-Li Rabbit Anti-Human TOLLIP Anti PPARgamma Human, Pol Anti Human AGO3, Monoclon Mouse anti Human IgG anti Rabbit Anti-MAL Myelin an Rabbit Anti-Human Toll In
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Preparation and preclinical assessment of folate-conjugated, radiolabelled antibodies.The folate receptor (FR) is frequently over-expressed on human cancer cells and may be a suitable target for radiopharmaceuticals. Because of FR expression in the kidneys, the rapidly renal clearing folate is not well suited as a carrier for therapeutic radionuclides. As an alternative, folate-immunoglobulin conjugates were studied as potential carriers for radionuclides.
1734 related Products with: Preparation and preclinical assessment of folate-conjugated, radiolabelled antibodies.Rabbit Anti-Rat Androgen Goat Anti-Human Androgen Native Human HSA (Low B12 Donkey anti Goat IgG (H + Mac3 antibody (FITC), Con Alkaline Phosphatase Co Native Bovine Folate Bind Rabbit Anti-Human Androge Texas Red Conjugated Affi Mouse Anti-Bovine Folate Polyclonal anti conjugate Folate antibody, Monoclon
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A competitive ELISA for albumin in rat urine.Nephropathy is characterized by urinary micro albumin. Rats are usually used in experimental studies. But, there was no specific and simple method for detecting rat urinary albumin. A specific, easily performed, and sensitive method for quantitatively determining rat urinary albumin is needed. Using rabbit anti-rat albumin polyclonal antibody, biotinylated goat anti-rabbit IgG, avidin conjugated peroxidase, and TMB (3,3',5,5'-tetramethylbenzidine) as substrate, a competitive ELISA for rat albumin in urine was developed. With this method, the urinary albumin in diabetic and normal rats was detected. This method was sensitive to 30 ng/mL and reproducibly quantifies rat urinary albumin levels in the range of 0.05-5 microg/mL. The rabbit anti-rat albumin polyclonal antibody showed no cross reaction with bovine and human serum albumins and rat IgG, and showed little cross-reaction with mouse albumin. The intra-assay CV was less than 10%. The urinary albumin markedly increased in diabetic rats. Since quantifying urinary albumin was very important in the experimental study of diabetic nephropathy, the results from our experiments indicated that this competitive ELISA could be used for quantification of rat urinary albumin.
Glucagon ELISA KIT, Rat G Beta Amyloid (42) ELISA K Beta Amyloid (1 40) ELISA Human Apolipoprotein AI E ELISA Agtr2,Angiotensin I Beta Amyloid (1 40) ELISA Mouse intercellular adhes Plant Indole 3 acetic aci Rat Albumin ELISA Kit Rabbit Anti-IAA (Indole-3 Rabbit Anti-Integrin alph Rat intestinal fatty acid
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An ELISA for the H-subunit of human ferritin which employs a combination of rabbit poly- and mice monoclonal antibodies and an enzyme labeled anti-mouse-IgG.We describe a sensitive ELISA for measuring the H-type subunit of human ferritin. A high detection sensitivity was attained by the use of antibodies from different species and an enzyme-conjugated secondary antibody. It consisted of a sandwich assay using a solid phase coated with a rabbit polyclonal antibody for human ferritin from term placenta and a soluble monoclonal antibody for human H-ferritin, followed by a secondary anti-mouse immunoglobulin (Ig)G conjugated to beta-galactosidase. The assay was calibrated with purified recombinant human H-ferritin from E. coli. The colorigenic chlorophenol red beta-D-galactopyranoside and the fluorogenic 4-methyl-umbelliferyl-beta-D-galactopyranoside substrates were used with similar outcome. The described method permits the measurement of human H-ferritin at a concentration ranging from 0.1 to 100 micrograms/l (or 20-20,000 pg per 200 microliters sample) and is accurate at a concentration as low as 0.3 microgram/l. The coefficient of variation of the assay was 6.05-10.3% and the recovery of H-ferritin added to cell lysates was 105.8 +/- 7.52%. Depending on the H-ferritin content of the cell line tested, only 600 to 60,000 cells of different human cell lines were needed to measure their H-ferritin content.
1777 related Products with: An ELISA for the H-subunit of human ferritin which employs a combination of rabbit poly- and mice monoclonal antibodies and an enzyme labeled anti-mouse-IgG.Rabbit Anti-Human Androge Proteins and Antibodies H Proteins and Antibodies H Proteins and Antibodies H Proteins and Antibodies H Proteins and Antibodies H Proteins and Antibodies H Proteins and Antibodies H Proteins and Antibodies H Proteins and Antibodies H Proteins and Antibodies H Proteins and Antibodies H
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Development of a polyclonal antibody with defined specificity against synthetic peptides from the N-myc oncoprotein using multiple antigen peptide and hemocyanin conjugation methods.The importance of determining N-myc oncoprotein rather than genomic N-myc amplification has been emphasized in neuroblastoma, especially in an international project to register biological risk factors in all neuroblastomas. A method to raise a specific polyclonal antibody against the N-myc oncoprotein in large quantities was sought using the synthetic antigen peptide and the multiple antigen peptide (MAP) method.
2386 related Products with: Development of a polyclonal antibody with defined specificity against synthetic peptides from the N-myc oncoprotein using multiple antigen peptide and hemocyanin conjugation methods.Polyclonal Antibody Synap Rabbit Anti-CCAP Cardioac Rabbit Anti-CCAP Cardioac Rabbit Anti-MYCBP2 Polycl Antigen-Antibody Pens, a Polyclonal Antibody Excha Androgen Receptor (Phosph Polyclonal Antibody Gluco Rabbit Anti-Mycobacterium Rabbit Polyclonal to Myco Rabbit Anti-Mycobacterium Multiple organ cancer tis
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alpha IIb beta 3 redistribution triggered by receptor cross-linking.Fibrinogen binding to alpha IIb beta 3 on adherent, spread platelets triggers active, cytoskeletally-directed redistribution of fibrinogen/alpha IIb beta 3 complexes on the platelet surface. Gold-conjugated fibrinogen, unlabeled, soluble fibrinogen, and individual fibrinogen molecules have been demonstrated to trigger receptor redistribution. Here we examine the respective roles of receptor cross-linking and ligand occupancy of receptors in initiating this movement. Monovalent, alpha IIb beta 3-binding fibrinogen fragments RGDS and HHLGGAKQAGDV did not trigger receptor redistribution, suggesting that ligand binding to a single receptor is an insufficient stimulus. Binding of monoclonal antibodies 10E5, AP2, and AP3 to the receptor did not trigger receptor movement. However, cross-linking these receptor-bound monoclonal antibodies by polyclonal anti-mouse IgG or by conjugation of the anti-receptor antibody to large colloidal gold particles triggered receptor redistribution identical in rate, pattern, and final distribution to that previously seen with fibrinogen binding. We conclude that receptor cross-linking provides the signal for initiation of fibrinogen/alpha IIb beta 3 complex redistribution on platelet surfaces.
Mouse AntiT cell receptor Rabbit Anti-14-3-3(Alpha Benzyl D-Glucopyranoside Rabbit Anti-14-3-3(Alpha Mouse anti human INF alph Rabbit anti Estrogen Rece Rat monoclonal anti mouse Rabbit Anti-IKK alpha + I Mouse Anti-Human Spectrin Mouse Anti-Human Estrogen Rabbit Anti-IKK alpha + I Retinoid X Receptor beta
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