Only in Titles

           Search results for: Mouse CRP ELISA Kit   

paperclip

#28961281   2017/09/29 Save this To Up

Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet.

Oxidative stress and inflammatory factors are deeply involved in progression of atherosclerosis. Mitochondrion-targeted peptide SS-31, selectively targeting to mitochondrial inner membrane reacting with cardiolipin, has been reported to inhibit ROS generation and mitigate inflammation. The present study was designed to investigate whether SS-31 could suppress the development of atherosclerosis in vivo.

2683 related Products with: Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet.

DNA (cytosine 5) methyltr Stat3 Peptide Inhibitor, Stat3 Peptide Inhibitor, Mouse Interleukin IL-31 I GLP 1 ELISA Kit, Rat Gluc C Peptide ELISA Kit, Rat Glucagon ELISA KIT, Rat G Anti 3 DG imidazolone Mon Heme Oxygenase-1 Blocking Insig1 Blocking Peptide Influenza A Virus Hemaggl L JNK Peptide Inhibitor I

Related Pathways

paperclip

#20661728   2010/09/08 Save this To Up

Novel solid-phase refolding method for preparation of scFv-immobilized polystyrene plates with high-antigen-binding activity.

In the present study, we demonstrated site-specific immobilization and solid-phase refolding of single-chain Fv antibodies on hydrophilic polystyrene (phi-PS) plates that was mediated by novel polystyrene binding peptides (PS-tags: RIIIRRIRR), which were originally isolated and optimized in previous studies. Three PS-tag-fused scFvs, namely scFv-PS, scFv-(PS), and scFv-PSII, which were over-expressed in the insoluble fraction of Escherichia coli cells were denatured and site-specifically immobilized onto hydrophilic PS plates in the presence of 0.5-4 M urea and 0.1% Tween 20. The antigen-binding activity of the scFvs was efficiently recovered by washing the surface of the plate with PBS that contained 0.1% Tween 20 (PBST). The solid-phase refolding mediated by PS-tag was successfully applied to several scFvs such as mouse anti-CRP antibodies and an anti-RNase antibody, although further investigation of the versatility of scFv-PSII is needed. The maximal density of PS-tag-fused scFvs was increased more than 15-fold compared with a whole monoclonal antibody (mAb) immobilized on Maxisorp and, consequently, the sensitivity of PS-tag-fused scFvs for CRP in a sandwich ELISA was increased 25-fold. Thus, the novel, solid-phase, refolding method mediated by a PS-tag will be very useful for preparation of solid supports coated with recombinant antibody fragments, which can be used in immunoassays and immuno-separation.

2599 related Products with: Novel solid-phase refolding method for preparation of scFv-immobilized polystyrene plates with high-antigen-binding activity.

EnzyChrom™ Kinase Assay HBV surface recombinant a MOUSE ANTI BORRELIA BURGD Dog Receptor-binding canc QuantiChrom™ Acetylchol QuantiChrom™ α-Amylase QuantiChrom™ Formaldehy EnzyChrom™ Catalase Ass EnzyChrom™ Phospholipas Mouse Anti-Ca19.9 Sialyl Mouse Anti-Lipoprotein Li P450 Demethylation Activi

Related Pathways