Search results for: Mouse IgG ELISA Kit
#28316427 2017/03/20 Save this To Up
Evaluation of Toxoplasma gondii soluble, whole and excretory/secretary antigens for diagnosis of toxoplasmosis by ELISA test.The present study was performed to compare the soluble, whole and excretory/secretary antigens of Toxoplasma gondii (RH strain) in diagnosis of toxoplasmosis by ELISA method. Tachyzoites of T. gondii, RH strain were injected in intra-peritoneal cavity of BALB/c mice, after 4 days tachyzoites were harvested by peritoneal washing of the mice. For soluble antigen, exudates were centrifuged and sediment sonicated and then centrifuged at 4 °C, 1 h, supernatant collected and density of protein determined by Bradford method. For whole antigen after collecting, washing and centrifuging of peritoneal fluid the tachyzoites sediment was counted. In excretory/secretary antigen 1.5 × 10(8) tachyzoites were transferred in 1 ml tube of saline and incubated under mild agitation and after centrifuging, supernatant was collected and protein density determined by Bradford method. 176 human serum samples were evaluated for T. gondii IgG antibody with prepared antigens, and finally serum samples were evaluated by commercial ELISA kit (Trinity, USA) which was considered as gold standard method. In this study sensitivity and specificity of prepared antigens compared with commercial kit in ELISA method. Sensitivity and specificity of soluble antigen was 91.4 and 74.5 %, in whole antigen these parameters were 77.1 and 77.3 % and in excretory/secretary antigen were 28.5 and 74.5 % respectively. Soluble antigen had high levels of sensitivity and specificity in ELISA method and the results were rather resemble to commercial kit (Trinity, USA).
2188 related Products with: Evaluation of Toxoplasma gondii soluble, whole and excretory/secretary antigens for diagnosis of toxoplasmosis by ELISA test.Toxoplasma gondii MIC 3 r Toxoplasma gondii P24 (GR Toxoplasma gondii P29 (GR Toxoplasma gondii P30 (SA TOXOPLASMA GONDII Culture Recombinant Toxoplasma go Recombinant Toxoplasma go Recombinant Toxoplasma go Recombinant Toxoplasma go Recombinant Toxoplasma go Recombinant Toxoplasma go ELISA TEK™ MBM Thermal
#27942363 2016/12/12 Save this To Up
Design of Indigenous ELISA Using Tachyzoites from the RH Strain of Toxoplasma gondii and Comparison with Commercial Kits in Ahvaz, Southwest of Iran, 2015.Toxoplasma gondii is one of the most common causes of latent infections in humans worldwide. Detecting anti-Toxoplasma antibodies in serum using serological tests is a common method to diagnose toxoplasmosis.
1327 related Products with: Design of Indigenous ELISA Using Tachyzoites from the RH Strain of Toxoplasma gondii and Comparison with Commercial Kits in Ahvaz, Southwest of Iran, 2015.Rat Inactive rhomboid pro Rabbit Anti-T. gondii RH Ofloxacin CAS Number [824 Toxoplasma gondii GRA8, r Toxoplasma gondii MIC 3 r Toxoplasma gondii P24 (GR Toxoplasma gondii P29 (GR Toxoplasma gondii P30 (SA TOXOPLASMA GONDII Culture Goat Anti-Human DHX9 RHA, Rat inositol 1,4,5,-trisp Rat TGF-beta-inducible ea
#27871277 2016/11/22 Save this To Up
Pulmonary endothelial activation caused by extracellular histones contributes to neutrophil activation in acute respiratory distress syndrome.During the acute respiratory distress syndrome (ARDS), neutrophils play a central role in the pathogenesis, and their activation requires interaction with the endothelium. Extracellular histones have been recognized as pivotal inflammatory mediators. This study was to investigate the role of pulmonary endothelial activation during the extracellular histone-induced inflammatory response in ARDS.
2015 related Products with: Pulmonary endothelial activation caused by extracellular histones contributes to neutrophil activation in acute respiratory distress syndrome.Stat3 Activation Inhibito EtBr Destaining Bag Kit A Anti-AICDA(Activation-ind Anti AICDA(Activation ind to FAPβ (Fibroblast Act to FAPβ (Fibroblast Act Goat Anti-Human Endotheli Recombinant Human Interfe Native Influenza HA (A To Native Influenza HA (A To Native Influenza HA (A To Cell Meter™ Fluorimetri
#26996337 2016/05/18 Save this To Up
A Novel IgM-capture enzyme-linked immunosorbent assay using recombinant Vag8 fusion protein for the accurate and early diagnosis of Bordetella pertussis infection.An ELISA that measures anti-PT IgG antibody has been used widely for the serodiagnosis of pertussis; however, the IgG-based ELISA is inadequate for patients during the acute phase of the disease because of the slow response of anti-PT IgG antibodies. To solve this problem, we developed a novel IgM-capture ELISA that measures serum anti-Bordetella pertussis Vag8 IgM levels for the accurate and early diagnosis of pertussis. First, we confirmed that Vag8 was highly expressed in all B. pertussis isolates tested (n = 30), but little or none in other Bordetella species, and that DTaP vaccines did not induce anti-Vag8 IgG antibodies in mice (i.e. the antibody level could be unaffected by the vaccination). To determine the immune response to Vag8 in B. pertussis infection, anti-Vag8 IgM levels were compared between 38 patients (acute phase of pertussis) and 29 healthy individuals using the anti-Vag8 IgM-capture ELISA. The results revealed that the anti-Vag8 IgM levels were significantly higher in the patients compared with the healthy individuals (P < 0.001). ROC analysis also showed that the anti-Vag8 IgM-capture ELISA has higher diagnostic accuracy (AUC, 0.92) than a commercial anti-PT IgG ELISA kit. Moreover, it was shown that anti-Vag8 IgM antibodies were induced earlier than anti-PT IgG antibodies on sequential patients' sera. These data indicate that our novel anti-Vag8 IgM-capture ELISA is a potentially useful tool for making the accurate and early diagnosis of B. pertussis infection.
1347 related Products with: A Novel IgM-capture enzyme-linked immunosorbent assay using recombinant Vag8 fusion protein for the accurate and early diagnosis of Bordetella pertussis infection.Bone Morphogenetic Protei Recombinant Human c-jun A Recombinant Human c-jun A Recombinant Human c-jun A Mouse Anti-RSV Fusion Pro Recombinant Human Androge Allergens, Phospholipase Recombinant Viral antige anti GSK3 Beta IgG2a (mon anti HIV 2 gp36 IgG1 (mon anti HIV 1 p24 IgG1 (mono anti HIV 1 p55 17 IgG1 (m
#26868748 2016/02/12 Save this To Up
Vascular Endothelial Growth Factor (VEGF) Concentration Is Underestimated by Enzyme-Linked Immunosorbent Assay in the Presence of Anti-VEGF Drugs.Commercially available enzyme-linked immunosorbent assay (ELISA) kits are often used to monitor vascular endothelial growth factor (VEGF) levels in exudative age-related macular degeneration. To test their accuracy, this study performed measurements using the ELISA kits in the presence of anti-VEGF drugs.
2221 related Products with: Vascular Endothelial Growth Factor (VEGF) Concentration Is Underestimated by Enzyme-Linked Immunosorbent Assay in the Presence of Anti-VEGF Drugs.Human Endocrine Gland Vas Human Vascular Endothelia Human Vascular Endothelia Mouse Vascular Endothelia Mouse Vascular Endothelia Rat Vascular Endothelial Mouse Vascular Endothelia Mouse Anti-Insulin-Like G Recombinant Human Vascula Rabbit Anti-VEGF Polyclon Rabbit Anti-VEGF Polyclon Rabbit Anti-VEGF Polyclon
#26788445 2016/01/20 Save this To Up
Measuring serum concentrations of interleukin-33 in atopic dermatitis is associated with potential false positive results.In the search for valid biomarkers in inflammatory diseases, cytokine serum concentrations are often measured by enzyme-linked immunosorbent assay and correlated to disease activity. Interleukin-33 is a relatively newly described cytokine, which holds a promising potential as a biomarker for different diseases including atopic dermatitis. However, interfering human anti-animal IgG antibodies and heterophilic antibodies might give rise to false positive or negative results that often go unnoticed.
2855 related Products with: Measuring serum concentrations of interleukin-33 in atopic dermatitis is associated with potential false positive results.Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti Human Interleukin-33 IL-3 interleukin 17 receptor C Recombinant Mouse Interle Human interleukin 2(IL-2) Single Donor Human Atopic ELISA Human , Interleukin Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s Sterile filtered rat ser
#26767257 2016/01/15 Save this To Up
[Study on immunologic function of thioredoxin glutathione reductase from Schistosoma japonicum].To study the immunogenicity and the immuno-protection of thioredoxin glutathione reductase from Schistosomajaponicum (SjTGR) against schistosome infection in mice.
1460 related Products with: [Study on immunologic function of thioredoxin glutathione reductase from Schistosoma japonicum].Glutathione Reductase Ass HT Glutathione Reductase Goat Anti-Human Thioredox Glutathione Reductase Flu Glutathione Reductase Act Glutathione Reductase Ass Glutathione Reductase Ass Thioredoxin Reductase Ass anti-Thioredoxin Reductas anti-Thioredoxin Reductas anti-Glutathione Reductas anti-Thioredoxin Reductas
#26419907 2015/10/20 Save this To Up
Versatile and Amplified Biosensing through Enzymatic Cascade Reaction by Coupling Alkaline Phosphatase in Situ Generation of Photoresponsive Nanozyme.The alkaline phosphatase (ALP) biocatalysis followed by the in situ enzymatic generation of a visible light responsive nanozyme is coupled to elucidate a novel amplification strategy by enzymatic cascade reaction for versatile biosensing. The enzymatic hydrolysis of o-phosphonoxyphenol (OPP) to catechol (CA) by ALP is allowed to coordinate on the surface of TiO2 nanoparticles (NPs) due to the specificity and high affinity of enediol ligands to Ti(IV). Upon the stimuli by CA generated from ALP, the inert TiO2 NPs is activated, which demonstrates highly efficient oxidase mimicking activity for catalyzing the oxidation of the typical substrate of 3,3',5,5'-tetramethylbenzidine (TMB) under visible light (λ ≥ 400 nm) irradiation utilizing dissolved oxygen as an electron acceptor. On the basis of the cascade reaction of ALP and the nanozyme of CA coordinated TiO2 (TiO2-CA) NPs, we design exquisitely colorimetric biosensors for probing ALP activity and its inhibitor of 2, 4-dichlorophenoxyacetic acid (2,4-DA). Quantitative probing of ALP activity in a wide linear range from 0.01 to 150 U/L with the detection limit of 0.002 U/L is realized, which endows the methodology with sufficiently high sensitivity for potentially practical applications in real samples of human serum (ALP level of 40-190 U/L for adults). In addition, a novel immunoassay protocol by taking mouse IgG as an example is validated using the ALP/nanozyme cascade amplification reaction as the signal transducer. A low detection limit of 2.0 pg/mL is attained for mouse IgG, which is 4500-fold lower than that of the standard enzyme-linked immuno-sorbent assay (ELISA) kit. Although only mouse IgG is used as a proof-of-concept in our experiment, we believe that this approach is generalizable to be readily extended to other ELISA systems. This methodology opens a new horizon for amplified and versatile biosensing including probing ALP activity and following ALP-based ELISA immunoassays.
2769 related Products with: Versatile and Amplified Biosensing through Enzymatic Cascade Reaction by Coupling Alkaline Phosphatase in Situ Generation of Photoresponsive Nanozyme.Amplite™ Fluorimetric A alkaline phosphatase (int Alkaline Phospatase (ALP) Monoclonal Anti-Alkaline Monoclonal Anti Alkaline SensiTek Alkaline Phosph SensiTek Alkaline Phosph SensiTek Alkaline Phosph SensiTek Alkaline Phosph SensiTek Alkaline Phosph UltraTek Alkaline Phosph UltraTek Alkaline Phosph
#26233088 2015/08/03 Save this To Up
Occurrence and maintenance of hantavirus infections among rodent populations in their natural habitat--results of a field study from Podkarpackie province, Poland 2010-2012.Human cases of hantavirus infection have been reported annually in Poland's Podkarpackie province, since 2007. In 2014 the number of cases reported significantly increased prompting a rise in studies focusing on the infection.
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#26108008 2015/06/25 Save this To Up
Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon.Toxoplasma gondii infection is widely prevalent in humans and other animals worldwide. Information on the prevalence of T. gondii infection is scarce in some regions of Brazil, including riverside communities along the Amazon River basin.
2334 related Products with: Epidemiological aspects of Toxoplasma gondii infection in riverside communities in the Southern Brazilian Amazon.Toxoplasma gondii GRA8, r Infection diseases: Heli Infection diseases: Heli Thermal Shaker with cooli FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Liver cancer tissue array Liver tissue cancer tissu
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