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#19379308   2009/04/21 Save this To Up

Calponin expression and myoepithelial cell differentiation in canine, feline and human mammary simple carcinomas.

Calponin is a 34-kDa smooth muscle-specific protein that has been shown to be a highly sensitive marker of myoepithelial cells in canine, feline and human mammary tissue and tumours. The expression of calponin was studied in 15 canine, 32 feline and 28 human simple mammary carcinomas using a monoclonal mouse antihuman calponin antibody and the avidin-biotin peroxidase complex (ABC) immunohistochemical technique. Calponin expression was compared with the expression of cytokeratin 14, a marker of normal mammary myoepithelial cells in the three species. Four different types of calponin-positive cells were identified: (1) Type 1: cytokeratin-14-positive pre-existing myoepithelial cells forming a continuous layer with images of focal disruptions; (2) Type 2: cytokeratin-14-positive isolated nests of fusiform, polygonal or round cells without atypia; (3) Type 3: cytokeratin-14-positive atypical cells indistinguishable from non-reactive atypical cells, which should have never been detected in haematoxylin and eosin-stained sections and (4) Type 4: cytokeratin-14-negative stromal fusiform cells around the neoplastic growth or cell nests, identified as myofibroblasts. Calponin-negative and cytokeratin-14-positive atypical neoplastic cells were observed in three canine, 28 feline and two human carcinomas. The latter were indicative of altered expression of high-molecular-weight cytokeratins in luminal epithelial-type simple carcinomas. Our findings show that calponin is a good marker of myoepithelial cell differentiation in feline, human and, particularly, canine simple carcinomas. The high number (six out of 15) of canine tumours with type 3 cells points to the need of both introducing calponin examination in the routine diagnostic schedule and performing further studies on its prognostic significance.

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Human Internal Mammary Ar GFP Expressing Human Inte Epidermal Growth Factor ( Epidermal Growth Factor ( CELLKINES Natural Human I Rabbit Anti-Human Androge Rabbit Anti-Human Androge Macrophage Colony Stimula Macrophage Colony Stimula Rabbit Anti-Cell death in Rabbit Anti-Cell death in Human Small Intestine Mic

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#9629667   1998/07/20 Save this To Up

Cross reactivities among some mammalian haptoglobins studied by a monoclonal antibody.

Mouse monoclonal antibody antihuman-Hp, product of clone 2.36.71.41 was found to recognize, however, with different affinities, the immunological determinant on haptoglobin of some mammals (goat, sheep, cow, horse, rabbit). The following differences in immunological cross reactivities were noticed: (i) haptoglobins present in sera of goat, sheep and cow (Artiodactyla, Bovidae) react in enzyme-linked immunosorbent assay (ELISA) and form precipitates in agarose gel; (ii) horse (Perissodactyla) and rabbit (Lagomorpha) haptoglobins react in ELISA, but they do not form precipitates; and (iii) haptoglobins of dog, fox, cat (Carnivora) and pig (Artiodactyla, Suidae) are not recognized by the tested monoclonal antibody either in ELISA or in agarose gel. This study suggests that monoclonal antibody, clone 2.36.71.41, recognizes the structure on haptoglobin around the disulphide bond linking two alpha chains. Antigenic structure of Bovidae haptoglobins is rather similar to human haptoglobin 2-2 (circular polymers) than to 2-1 type (linear polymers). Monoclonal antibody 2.36.71.41 could be used for classification of mammalian haptoglobins by epitope structure. It can distinguish polymeric haptoglobins similar to human type 2-2 from other mammalian haptoglobins.

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