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[Determination of the immunoglobulin G supply in the newborn calf].

A sufficient supply of colostral antibodies within the first hours of life is crucial for the development and the health status in young calves. It is rational to examine the immunoglobulin uptake of single animals, but particularly on a herd basis, during herd controls and consultations. This enables economical calf rearing in accordance with animal welfare. Because of the costly, laboratory-dependent and in part time-consuming direct measurement of the absorbed immunoglobulins using radial immunodiffusion (RID) or ELISA, multiple studies attempted to develop indirect methods, which would be affordable and operational in the field. These aim to draw an inference for the absorbed quantity of colostral antibodies based on other correlated parameters. Multiple validations showed in part significant differences between various methods concerning specificity and sensitivity in comparison to the direct methods. In addition to RID and ELISA, this article presents the measurement of the γ-glutamyltransferase (GGT) activity, the determination of the total serum protein concentration using refractometry and the zinc sulphate turbidity test, and describes the advantages and disadvantages of their application. Refractory measurement and determination of the GGT activity represent a valuable alternative to a laboratory-dependent immunoglobulin G measurement. Nevertheless, there is no ideal rapid test method, such that several influencing factors have to be considered.

2370 related Products with: [Determination of the immunoglobulin G supply in the newborn calf].

Multiple organ tumor tiss MultiGene Gradient therm MyGenie 96 Gradient Therm MyGenie 96 Gradient Therm Thermal Shaker with cooli FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu CSL Gradient Thermal Cycl

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Cluster binding studies with two anti-Thomsen-Friedenreich (anti-core-1, CD176, TF) antibodies: Evidence for a multiple TF epitope.

Antibodies to carbohydrate epitopes are often of the IgM isotype and require multiple binding for sufficient avidity. Therefore clusters of epitopes are preferred antigenic sites in these cases. We have examined the type of clusters recognized by two anti-Thomsen-Friedenreich (TF, core-1, CD176) IgM antibodies, NM-TF1 and NM-TF2, using several different sets of TF-carrying synthetic glycoconjugates in ELISA experiments. To our surprise, the single most important factor determining binding strength was a close vicinity of several TF glycans at distances of ≤1 nm. Considering the known dimensions of IgM antibodies, our data strongly suggest that a cluster of up to four TF moieties, presenting as a "multiple epitope", is required to attach to a single combining site in order to result in adequate binding strength. This effect can also be achieved by "surrogate-multiple epitopes" consisting of separate TF-carrying molecules in close vicinity. In addition, it was found that serine-linked TFs are stronger bound than threonine-linked TFs by both antibodies. This peculiar type of cluster recognition may contribute to improved avidity and explicit tumor specificity.

2127 related Products with: Cluster binding studies with two anti-Thomsen-Friedenreich (anti-core-1, CD176, TF) antibodies: Evidence for a multiple TF epitope.

anti HBcAg core IgG2a (mo anti HBcAg core IgG2b (mo anti HBcAg core IgG2b (mo anti HBcAg core IgG2a (mo anti HBcAg core IgG2b (mo anti HBcAg core IgG2a (mo Goat Anti- TFAP2D, (inter Goat Anti-Rat Clusterin A Mouse Anti-Human CG beta Mouse Anti-Human CG beta Mouse Anti-HCV Core Antig Mouse Anti-HCV Core Antig

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Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children.

Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children. Identification of signs typical for cholesteatoma and support of retraction theory of cholesteatoma.

2060 related Products with: Immunohistochemical analysis of retraction pocket pars tensa of tympanic membrane in children.

Nuclear Membrane Receptor Angiogenesis (Human) Anti Angiogenesis (Human) Anti Angiogenesis (Mouse) Anti Apoptosis (Human) Antibod Atherosclerosis (Human) A Atherosclerosis (Mouse) A Chemokine (Human) Antibod Cytokine (Human) Antibody Cytokine (Human) Antibody Cytokine (Human) Antibody Cytokine (Human) Antibody

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Multicenter evaluation of a novel ROS1 immunohistochemistry assay (SP384) for detection of ROS1 rearrangements in a large cohort of lung adenocarcinoma patients.

The detection of a ROS1 rearrangement in advanced and metastatic lung adenocarcinoma (LUAD) lead to a targeted treatment with tyrosine kinase inhibitors, with favorable progression-free survival and overall survival of the patients. Thus, it is mandatory to screen for the ROS1 rearrangement in all these patients. ROS1 rearrangements can be detected using break-apart fluorescence in situ hybridization (FISH), which is the "gold standard"; however, ROS1 immunohistochemistry (IHC) can be used as a screening test since it is widely available, easy and rapid to perform, and cost-effective.

2032 related Products with: Multicenter evaluation of a novel ROS1 immunohistochemistry assay (SP384) for detection of ROS1 rearrangements in a large cohort of lung adenocarcinoma patients.

Lung adenocarcinoma and n Lung adenocarcinoma tissu Lung adenocarcinoma tissu Lung adenocarcinoma tissu Multiple lung carcinoma ( Lung large cell carcinoma MarkerGeneTM Fluorescent Amplite™ Fluorimetric H Amplite™ Intracellular Amplite™ Fluorimetric P Amplite™ Fluorimetric A Cell Meter™ Intracellul

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Perioperative management of a patient with severe factor V deficiency presenting with chronic subdural hematoma: a clinical report.

Severe factor V deficiency is an extremely rare coagulation disorder. Patients with factor V activity below 5% usually become symptomatic in early childhood.

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The Formation and Effect of Mannitol Hemihydrate on the Stability of Monoclonal Antibody in the Lyophilized state.

Crystalline bulking agent in lyophilized biopharmaceutical formulations provides an elegant lyophilized cake structure and allows aggressive primary drying conditions. The interplay between amorphous and crystalline state of excipients heavily influence the stability of lyophilized biological products and should be carefully evaluated in the formulation and process development phase. This study focuses on: (1) elucidating the influence of formulation and lyophilization process variables on the formation of different states of mannitol and (2) its impact on model monoclonal antibody stability when compared to sucrose. The main aim of the present research work was to study the influence of different mannitol to sucrose ratios and monoclonal antibody concentrations on mannitol physical form established during lyophilization. In addition, also the effect of process variables on mannitol hemihydrate (MHH) formation was under investigation. Thermal analysis and powder X-ray diffraction results revealed that the ratio between sucrose and mannitol and mAb concentration have a decisive impact on mannitol crystallization. Namely, increasing amount of mannitol and monoclonal antibody resulted in decreasing formation of MHH. From the process parameters investigated, a higher secondary drying temperature has the biggest impact on the complete dehydration of MHH. Specifically, higher secondary drying temperature reflected in complete dehydration of MHH. Annealing temperature was shown to affect the MHH content in the final product, wherein the higher annealing temperature was preferential for formation of anhydrous mannitol. Temperature stress stability study revealed that the most important parameter influencing monoclonal antibody stability is the ratio of protein to sucrose. Contrary to widespread assumption, we did not detect any impact of MHH on the stability of the investigated monoclonal antibody.

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Diminished cytolytic activity of γδ T cells with reduced DNAM-1 expression in neuroblastoma patients.

Neuroblastoma is one of the children's malignant tumors with poor prognosis, as well as high recurrence and metastasis rates after surgical removal and chemotherapy. γδ T-cell based immunotherapy receives increasing attention thanks to their strong cytolytic activity to tumor cells. Our previous data revealed a significant increase in circulating γδ T-cell frequency in NB patients. In the present study, we found that beside a reduction of IFN-γ in serum of NB patients, DNAM-1 expression decreased in both circulating and PAM-expanded NB γδ T cells. Upon PAM stimulation, NB γδ T cells showed a reduced level of cell proliferation. In addition, the cytolytic activity of NB γδ T cells to NB cell lines was proved to be attenuated in a co-culture system. The fact that DNAM-1 neutralizing antibody abolished the tumor cell killing accentuates the indispensable role of DNAM-1 molecule in γδ T-cell cytolytic function.

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HIV Self Test Kit, 1Test Cell Meter™ Fluorimetri Cell Meter™ Fluorimetri Alkaline Phospatase (ALP) Glucagon ELISA KIT, Rat G Leptin ELISA Kit, Rat Lep Sf9 insect cells Sf21 insect cells superSf9-1 insect cells superSf9-2 insect cells superSf9-3 insect cells Tubastatin A Mechanisms:

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Anti-atherogenic effects of CD36-targeted epigallocatechin gallate-loaded nanoparticles.

Intimal macrophages play a critical role in atherosclerotic lesion initiation and progression by taking up oxidized low-density lipoprotein (oxLDL) and promoting inflammatory process. 1-(Palmitoyl)-2-(5-keto-6-octene-dioyl) phosphatidylcholine (KOdiA-PC), a major type of oxidized phosphatidylcholines (PC) found on oxLDL, has a high binding affinity to the macrophage scavenger receptor CD36 and participates in CD36-mediated recognition and uptake of oxLDL by intimal macrophages. We successfully synthesized epigallocatechin gallate (EGCG)-loaded nanoparticles (Enano), which were composed of EGCG, PC, (+) alpha-tocopherol acetate, and surfactant. We also incorporated KOdiA-PC on the surface of Enano to make ligand-coated Enano (L-Enano) to target intimal macrophages. The objectives of this study were to determine the anti-atherogenic effects of Enano and L-Enano in LDL receptor null (LDLr-/-) mice. Our in vitro data demonstrated that L-Enano had a higher binding affinity to mouse peritoneal macrophages than Enano. This high binding affinity was diminished by CD36 antibodies or knockdown of the CD36 receptor in mouse peritoneal macrophages, confirming the specific binding of L-Enano to the macrophage CD36 receptor. LDLr-/- mice were randomly divided to six groups and received weekly tail vein injection with PBS, EGCG, void nanoparticles (Vnano), Enano, ligand-coated Vnano (L-Vnano), or L-Enano once per week for 22 weeks. The dose of EGCG was 25 mg per kg body weight. L-Enano at 20 μg/mL significantly decreased production of monocyte chemoattractant protein-1, tumor necrosis factor alpha, and interleukin-6 from mouse macrophages, while having no effect on their plasma levels compared to the PBS control. There were no significant differences in blood lipid profiles among six treatment groups. Mice treated with L-Enano also had significantly smaller lesion surface areas on aortic arches compared to the PBS control. Liver EGCG content was decreased by treatments in the order of EGCG>Enano>L-Enano. Native EGCG had inhibitory effects on liver and body fat accumulation. This molecular target approach signals an important step towards inhibiting atherosclerosis development via targeted delivery of bioactive compounds to intimal macrophages.

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Rat Anti-Mouse CD36 Rat Anti-Mouse CD36, FITC Rat Anti-Mouse CD36, RPE- Rabbit Anti-CD36 PAS-4 Po Rabbit Anti-CD36 PAS-4 Po Rabbit Anti-CD36 PAS-4 Po Rabbit Anti-CD36 PAS-4 Po Rabbit Anti-CD36 PAS-4 Po Rabbit Anti-CD36 PAS-4 Po Rabbit Anti-CD36 PAS-4 Po Rabbit Anti-CD36 PAS-4 Po Rabbit Anti-CD36 PAS-4 Po

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Dysregulation of Akt-FOXO1 pathway leads to dysfunction of regulatory T cells in psoriasis patients.

Psoriasis is a T lymphocyte-driven systemic inflammatory disease. Regulatory T (Treg) cells are essential for establishing and maintaining immune tolerance. In this study, we found that the psoriatic patients and healthy controls had comparable percentages of circulating CD4CD25FOXP3 Treg cells, but psoriatic Treg cells had reduced suppressive function. Then, mRNA arrays were performed to study the gene expression profile of psoriatic Treg cells. Interestingly, psoriatic Treg cells expressed high level of Th1-like transcription factor and cytokines such as T-bet and IFN-γ. Further, we found that FOXO1 can bind to the promoter of TBX21 to inhibit its expression, thus keeping the suppressive function of Treg cells. However, in psoriatic Treg cells, Akt-mediated phosphorylation of FOXO1 was increased, which subsequently caused FOXO1 inactivation by nuclear exclusion. In addition, incubation of healthy Treg cells with psoriatic serum led to Akt activation, FOXO1 nuclear exclusion and the loss of FOXO1 transcription activity. The role of FOXO1 in regulating the function of Treg cells was corroborated using a psoriasis-like mouse model in which Foxo1-deficient Treg cells failed to protect mice from developing psoriasis. In conclusion, our findings reveal that the dysregulation of Akt-FOXO1 pathway may be a critical cause of Treg cell dysfunction in psoriasis.

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Development of a Bead-based Immunoassay Using Virus-like Particles for Detection of Alphaviral Humoral Response.

There is a pressing need for sustainable and sensitive immunodiagnostics for use in public health efforts to understand and combat the threat of endemic and emerging infectious diseases. In this proof-of-concept work, we describe an immunodiagnostic approach based on the utilization of virus-like particles (VLPs) in a magnetic bead-based platform for multiplexed detection of antiviral humoral response. A retroviral-based VLP, that presents Venezuelan equine encephalitis virus E1/E2 glycoprotein antigen on its surface, was synthesized and coupled to magnetic beads to create VLP-conjugated microspheres (VCMs). Using these VCMs, IgM and IgG antibodies were detectable in nonhuman primate (NHP) and human clinical serum samples at dilutions of 1 × 10 [4] and greater. We also extended the VCM methodology to an Old World alphavirus, chikungunya virus, demonstrating the flexibility of this approach toward different VLP architectures. When multiplexed on the MAGPIX® platform, this method provided differential detection between Old World and New World alphaviral IgM. This flexible, immunodiagnostic method, based on the MAGPIX® platform, demonstrates compatibility of particulate antigens with bead-based assays, improves sensitivity by up to 2-logs, and has faster sample-to-answer time over traditional methods.

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MarkerGeneTM Fluorescent Custom Immunoassay Develo MOUSE ANTI CANINE DISTEMP Adeno Associated Virus (A Adeno Associated Virus (A Recombinant Viral Antige Tick born encephalitis vi Rubella virus E1 mosaic r Rubella virus E2 recombin Rubella virus capsid (C) West Nile Virus Envelope West Nile Virus Pre M rec

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