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Search results for: QuantiChrom™ Calcium Assay Kit

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#34382118   2021/08/11 To Up

Characterization of a clonal human periodontal ligament stem cell line exposed to methacrylate resin-, bioactive glass-, or silicon-based root canal sealers.

The aim of this study was to characterize a clonal human periodontal ligament (PDL) stem cell line (line 2-23 cells) cultured with root canal sealers based on methacrylate resin (SuperBond sealer; SB), bioactive glass (Nishika Canal Sealer BG; BG), or silicon (GuttaFlow 2; GF). The sealers were set in rubber molds to form sealer discs. Line 2-23 cells were cultured with or without the discs for 3 days. The cell viability was evaluated by direct cell counting and MTT assay. Inflammation-, PDL-, collagen-, and cell cycle-related gene expression was investigated by real-time RT-PCR. Collagen production was analyzed by Picro Sirius Red staining. Calcium ion concentration in the culture was measured by a QuantiChrom calcium assay kit. Line 2-23 cells survived when cultured with GF discs, but decreased cell viability was observed with SB and BG discs. The expression of inflammation-related genes was higher in cells cultured with SB discs, and expression of PDL-related genes was lower in cells exposed to SB and BG discs. These discs also down-regulated collagen production in line 2-23 cells. BG discs increased calcium ion concentration in the culture medium. Cells exposed to GF discs exhibited the same inflammation-, PDL-, collagen-, and cell cycle-related gene expression and collagen production as untreated cells. These results suggested that the characteristics of line 2-23 cells cultured with GF discs was highly resemble to untreated cells throughout the 3 days of the culture model.
Atsushi Tomokiyo, Daigaku Hasegawa, Taiga Ono, Ryoko Nagano, Keita Ipposhi, Kozue Yamashita, M Anas Alhasan, Hidefumi Maeda

2077 related Products with: Characterization of a clonal human periodontal ligament stem cell line exposed to methacrylate resin-, bioactive glass-, or silicon-based root canal sealers.

1 kit1 mgOne 96-Well Microplate Ki25 4 Arrays/Slide1 moduleOne 96-Well Microplate Ki

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#31099212   2018/11/15 To Up

Release of calcium ions from particulate monosodium titanates for dental mineralization applications.

The calcium ion [Ca(II)] release from monosodium titanates (MST) complexed to calcium ions [Ca(II)], referred to as MST-Ca(II), was examined under varying incubation times, pH conditions, and ion equilibrium disruptions.
Jeanie L Drury, Yen-Wei Chen, Bryce J Plancich, Kathryn Ml Taylor-Pashow, David T Hobbs, John C Wataha

1513 related Products with: Release of calcium ions from particulate monosodium titanates for dental mineralization applications.

0.1 ml1 ml250 ml25 µg0.2 mg1 LITRE25 µg100 TESTS0.1 mg100 ml0.25 mg0.1 mg

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