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#35498979   2022/01/15 To Up

Preparation, chemical structure, and immunostimulatory activity of a water-soluble heteropolysaccharide from fruiting bodies.

A water-soluble heteropolysaccharide (SGP2-1) was purified from fruiting bodies by anion-exchange chromatography and gel permeation chromatography. The structural characteristics were analyzed by high-performance gel permeation chromatography, high-performance liquid chromatography, Fourier transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy. The immunostimulatory activity was investigated using RAW 264.7 macrophages. Results showed that SGP2-1 with weight average molecular weight of 150.75 kDa was composed of mannose, glucose, and xylose. The backbone of SGP2-1 was mainly composed of → 4)--Glc-(1→, and the terminal group -d-Glc → was linked to the main chain by -6 position. SGP2-1 could significantly enhance pinocytic capacity, reactive oxygen species production, and cytokines secretion. SGP2-1 exerted immunomodulatory effects through interacting with toll-like receptor 2, and activating mitogen-activated protein kinase, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways. These findings indicated that SGP2-1 could be explored as a potential immunomodulatory agent for application in functional foods.
Xiong Gao, Ranhua Zeng, Chi-Tang Ho, Bin Li, Shaodan Chen, Chun Xiao, Huiping Hu, Manjun Cai, Zhongzheng Chen, Yizhen Xie, Qingping Wu

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