Search results for: Rabbit Anti-HIPK2 Polyclonal Antibody, FITC conjugated,Isotype: IgG
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Production and characterization of anti-human IgG F(ab')2 antibody fragment.In present study an optimized protocol for the separation of antibodies into antigen-binding fragments F(ab')2 using pepsin digestion was investigated. The production of these fragments is a consequential step in the development of medical research, treatment and diagnosis. For production of polyclonal antibody rabbit received antigen in four steps. The rabbit serum at 1/128000 dilution showed high absorbance in reaction with human IgG at the designed ELISA method. Rabbit IgG was purified by Ion-Exchange Chromatography (IEC) method. Purity was assessed by SDS-PAGE method. In non-reduced condition only one band was seen in about 150 kDa MW position and in reduced form, two bands were seen in 50 and 25 kDa MW positions. Rabbit IgG was digested by pepsin enzyme. The antibody fragments solution was applied to Gel filtration column to isolate the F(ab')2. Non-reduced SDS-PAGE for determining the purity of F(ab')2 fragment resulted in one band in 100 kDa corresponds to F(ab')2 fragment and a band in 150 kDa MW position corresponds to undigested IgG antibodies. The activities of FITC conjugated F(ab')2 fragment and commercial ones were compared using flowcytometry method. The activity results implied that the FITC conjugated- anti human F(ab')2 fragment worked as efficiently as the commercial one.
2515 related Products with: Production and characterization of anti-human IgG F(ab')2 antibody fragment.Rabbit Anti-Cell death in Rabbit Anti-CIDE A Polycl Mouse anti Human IgE anti Rabbit Anti-Ankyrin eryth Rabbit Anti-ATAD5 Polyclo Rabbit Anti-NRH2 Polyclon Mouse anti Human IgG anti Rabbit Anti-SEN1 Polyclon Mouse anti-human type II Mouse anti-human type II Rabbit Anti-THYN1 Polyclo Rabbit Anti-KCNMB1 Maxi P
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Chi-Ju-Di-Huang-Wan protects rats against retinal ischemia by downregulating matrix metalloproteinase-9 and inhibiting p38 mitogen-activated protein kinase.Retinal ischemia is a retinal disorder related to retinal vascular occlusion, glaucoma, diabetic retinopathy and age-related macular degeneration. The study aimed to evaluate the protective effects and underlying mechanisms of Chi-Ju-Di-Huang-Wan (CJDHW) against retinal ischemia in rats.
1710 related Products with: Chi-Ju-Di-Huang-Wan protects rats against retinal ischemia by downregulating matrix metalloproteinase-9 and inhibiting p38 mitogen-activated protein kinase.Mouse Anti-Human Matrix M Bovine Activated Protein Activated Carrier Protei Human Matrix metalloprote Recombinant SARS Virus Ma Recombinant Human p38a SA to PKA--RIIA (Protein Ki Anti-ATM Protein Kinase p Polyclonal Antibody Prote G Protein Coupled Recepto Anti ATM Protein Kinase p Anti ATM Protein Kinase p
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Detection of 3-chlorinated tyrosine residues in human cells by flow cytometry.Hypochlorite is a strong oxidant, generated under pathological conditions, with the potency to introduce chlorine atom into a number of molecules. 3-Chloro- and 3,5-dichlorotyrosine are documented to be generated by this oxidant and their elevated levels were found in many diseases. Thus, we decided to check the possibility of use of FITC-conjugated antibodies for flow cytometric detection of 3-chlorotyrosine residues in human cells (A549, MCF-7, HUVEC-ST) exposed to the action of hypochlorite. Additionally, we compared the effects of chlorohydrins and N-chloroamino acids as chlorine donors. Cell fixation and permeabilization was followed by incubation with rabbit polyclonal anti-3-chlorotyrosine primary antibody and subsequent staining with goat anti-rabbit FITC-labeled secondary antibody. For antibody isotypic control, normal rabbit IgG was employed. Hypochlorite appeared to be the most efficient from the chlorocompounds analyzed in chlorotyrozine generation in all cell lines. Statistically significant increase of fluorescence corresponding to the level of 3-chlorotyrosine residues was found in cells treated with hypochlorite even at non-toxic concentrations (<5μM). This effect was not observed in cells exposed to the action of chlorinated amino acids or chlorohydrins. The use of anti-3-chlorotyrosine antibodies in conjunction with fluorophore-conjugated secondary antibodies analysis allows for detection of 3-chlorotyrosine residues by flow cytometry in cells treated with low doses of hypochlorite.
1224 related Products with: Detection of 3-chlorinated tyrosine residues in human cells by flow cytometry.Rabbit Anti-intestinal FA Human Internal Mammary Ar Rabbit Anti-TNIP2 ABIN2 T Macrophage Colony Stimula Rabbit Anti-Cell death in Macrophage Colony Stimula Cell Meter™ Intracellul Human Large Intestine Mic Rabbit Anti-APIP Apaf1 In Rabbit Anti-TNIP2 ABIN2 T GFP Expressing Human Inte MarkerGeneTM in vivo lacZ
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Sensitive immunoassay of Listeria monocytogenes with highly fluorescent bioconjugated silica nanoparticles probe.In this paper, a sensitive immunoassay method was proposed for Listeria monocytogenes detection by using highly fluorescent bioconjugated nanoparticles probe. (FITC-IgG)-doped fluorescent silica nanoparticles (fsNPs) firstly were synthesized by a microemulsion method and characterized by TEM and fluorescent spectra. Then the prepared fsNPs were conjugated with polyclonal rabbit anti-L. monocytogenes antibody (pAb) and used as indicator probe. A sandwich-type immune affinity reaction between polyclonal rabbit anti-L. monocytogenes antibody coated onto microplate wells, target bacteria and the fsNPs-antibody conjugates subsequently was conducted to detect target L. monocytogenes and assemble the indicator probe onto the wells. The target L. monocytogenes was measured by the fluorescent signals of the assembled indicator probes. Under the optimal conditions, the calibration graph of fluorescent intensity is proportional to the amount of target bacteria over the range of 50-10,320 CFU/mL with a detection limit of 50 CFU/mL. The proposed method has been successfully applied to detect L. monocytogenes in food samples offering the advantages of sensitivity, simplicity, and stability.
1114 related Products with: Sensitive immunoassay of Listeria monocytogenes with highly fluorescent bioconjugated silica nanoparticles probe.8 Octadecyloxypyrene 1,3, 3,3 dioctadecyloxacarbocy 4 Methylumbelliferyl sulf Fluorescein mono beta D G Fluorescein di beta D gal Highly Sensitive 8 OHdG C Mouse Anti-Listeria monoc 11 (4,4 difluoro 5,7 dime 6 para Toluidino 2 naphth Fluorescein 5 thiosemicar N,N,N Trimethyl 4 (6 phen Beta Amyloid (1 42) High
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Combination of immunomagnetic separation with flow cytometry for detection of Listeria monocytogenes.Listeria monocytogenes can grow at the low temperature commonly used in the storage and transportation of food, and the number of cases of food poisoning caused by L. monocytogenes has increased recently in the US and Europe. Several methods of detecting L. monocytogenes cells have been proposed; however, all existing methods require approximately 48 h incubation. In this study, we attempted rapid detection of L. monocytogenes using flow cytometry (FCM). The method is based on measuring the number of L. monocytogenes cells by using a combination of FCM and immunomagnetic separation (IMS). First, polyclonal antibodies (anti-L. monocytogenes rabbit IgG-FITC) conjugated with fluorescein isothiocyanate (FITC) were reacted with L. monocytogenes cells, and then FCM was applied. The cell numbers were determined by FCM using a traditional colony-counting method in the range of 10(4)-10(8) cells ml(-1). Tetrameric antibody complexes (TAC) were used because they can recognize both magnetic and FITC molecules on the FITC-conjugated antibodies. FITC-labeled L. monocytogenes cells were reacted with a secondary antibody (TAC) bound to magnetic beads. Then, IMS was used. The method is suitable for detection in the range of 10(2)-10(8)cells ml(-1). The FCM assay enumerated the cells within 1 min and the total assay time, including sample preparation, was less than 2 h.
2121 related Products with: Combination of immunomagnetic separation with flow cytometry for detection of Listeria monocytogenes.LISTERIA MONOCYTOGENES se Cell Meter™ Annexin V B Cell Meter™ Fluorimetri Mouse Anti-Listeria monoc Cell Meter™ Generic Flu Cell Meter™ Phosphatidy Ofloxacin CAS Number [824 Nano Fluorescent Size Sta Flow Cytometry Staining B Cell Meter™ NIR Mitocho Formaldehyde Detection Ki Cell Meter™ Annexin V B
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Detection of Xylella fastidiosa from resistant and susceptible grapevine by tissue sectioning and membrane entrapment immunofluorescence.Immunofluorescence detection was performed by tissue sectioning and membrane entrapment of Xylella fastidiosa from the inoculated hybrid selection F8909-08 (Vitis rupestris A. de Serres x V. arizonica/candicans b43-17; resistant) and Chardonnay (susceptible). In both techniques, tissue sections and bacteria-trapped polycarbonate membranes were incubated with specific polyclonal IgG and stained with fluorescein isothiocyanate (FITC)-conjugated IgG from rabbits to X. fastidiosa cells. The stained preparations were observed by fluorescence microscopy. Rapid identification of the bacteria within 3 weeks post inoculation (wpi) was possible in thin cross sections of the petioles, which allowed penetration of the specific antibody. Examination of the bacteria over time was also possible, and allowed observation of bacterial multiplication and invasion of xylem vessels. The membrane entrapment technique was able to isolate bacteria at low concentrations in infected but asymptomatic plants.
1526 related Products with: Detection of Xylella fastidiosa from resistant and susceptible grapevine by tissue sectioning and membrane entrapment immunofluorescence.Rabbit Anti-Human Androge Androgen Receptor Antibod 5α-N-Acetyl-2'H-androst- Androsta-1,4,6-triene-3,1 Recombinant Human Androge Epiandrosterone (3 beta H Androstane-3a,17b-diol Gl Rabbit anti Androgen Rece 4 Androstene 3,17 dione C Androgen Receptor (Ab 650 Androgen Receptor (Phosph Androgen Receptor , Mouse
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Removal of therapeutic anti-lymphocyte antibodies from human sera prior to anti-human leukocyte antibody testing.Both monoclonal (e.g. Orthoclone (OKT3), rituximab) and polyclonal (e.g. ATGAM, Thymoglobulin (Thymo)) anti-lymphocyte Abs (ALAs) are used extensively in organ transplantation for immunosuppression induction, desensitization, and treatment of acute rejection. ALAs often interfere with post transplant immunologic monitoring. We describe a method that uses magnetic beads to selectively remove ALAs from patient serum. Rabbit anti-mouse Fc-specific (180 mug), or rabbit anti-mouse Fab-specific (180 microg), or rabbit anti-horse heavy and light chain-specific and rabbit anti-horse F(ab')2 (200 microg) (Jackson Immunoresearch) was adsorbed to 6.7 x 10(8) Dynabeads M-280 conjugated with sheep anti-rabbit IgG (Dynal Biotech). Fifty microliters of normal human serum (NHS) with 2 microg/ml of OKT3 or 100 microg/ml ATGAM, Thymo, or rituximab were incubated with conjugated beads for several incubations. NHS containing ALAs before and after treatment by the protocol were incubated with human lymphocytes and labeled with FITC-antibody to immunoglobulin of the species used to produce the particular ALA. Residual ALA was determined using flow cytometry. Average median channel for serum with or without ALA was 11.1 and 0.120, respectively for OKT3; 64.4 and 0.344 for ATGAM; 108.5 and 0.200 for Thymo; and 1022.5 and 11.4 for rituximab. Treatment lowered the median channel for serum with OKT3 to 0.103, 0.309 for ATGAM, 0.199 for Thymo, and 12.1 for rituximab. ALAs can be effectively removed from serum by the use of magnetic beads conjugated with Ab specific for ALA thereby permitting immunologic monitoring without interference.
1688 related Products with: Removal of therapeutic anti-lymphocyte antibodies from human sera prior to anti-human leukocyte antibody testing.Mouse anti Human IgE anti Mouse anti Human IgG anti Mouse Anti-Human CD37 (B- Anti-AdipoR1 Human Polycl Anti AGO2 Human, Monoclon Mouse anti Human IgA anti Mouse anti Human IgM anti Rabbit Anti-Human TOSO (C Anti AGO2 Human, Monoclon Mouse anti Human IgE anti Anti Galectin(Gal-3) Huma Mouse Anti-Human CD103 (I
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Dual enhancement of triple immunofluorescence using two antibodies from the same species.Triple immunofluorescence method with two mouse monoclonal antibodies and another rabbit polyclonal antibody was established with catalyzed reporter deposition (CARD) amplification on thick floating sections from the rat cerebellum. One of the monoclonal antibodies (anti-calbindin), diluted maximally, probed with anti-mouse IgG-horseradish peroxidase (HRP) and amplified with Cy5-conjugated tyramide, immunolabeled cerebellar Purkinje cells and their arborization. Subsequently, a rabbit polyclonal IgG (anti-glial fibrillary acidic protein (anti-GFAP)), probed with anti-rabbit IgG-HRP, amplified with biotin-tyramide and visualized with fluorescein-isothiocyanate (FITC)-streptavidin, immunolabeled Bergmann's glia. Another mouse monoclonal IgG (anti-SNAP25), probed with anti-mouse IgG-rhodamine without CARD amplification, selectively visualized synaptic sites, because the maximal dilution of the other monoclonal antibody (anti-calbindin) was below the detection threshold of this anti-mouse IgG-rhodamine. Separation of the two signals (calbindin and SNAP25), each detected through mouse monoclonal antibody, was then based on the difference of sensitivity either with or without CARD amplification. Triple immunofluorescence is possible when just one of the three primary antibodies is from different species. Intensification of two of the three signals provides further advantages to examine immunolocalization of multiple epitopes on histological sections.
2389 related Products with: Dual enhancement of triple immunofluorescence using two antibodies from the same species.Rabbit Anti-Theophylline Multiple organ cancer tis Mouse monoclonal anti-fla Goat Anti-Human Dual oxid Mouse Anti-SARS Nucleocap Anti-SARS Nucleocapsid Pr Mouse Anti-Bacteroides th Anti-SARS Spike Protein I Sheep Anti-Theophylline 3 Multiple organ tumor tiss Rat Anti-CCT theta Antibo Anti-Human NaPi-2 NPT2a I
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In situ localization associates biologically active plant natriuretic peptide immuno-analogues with conductive tissue and stomata.Plant natriuretic peptide immuno-analogues (irPNP) have previously been shown to affect a number of biological processes including stomatal guard cell movements, ion fluxes and osmoticum-dependent water transport. Tissue printing and immunofluorescent labelling techniques have been used here to study the tissue and cellular localization of irPNP in ivy (Hedera helix L.) and potato (Solanum tuberosum L.). Polyclonal antibodies active against human atrial natriuretic peptide (anti-hANP) and antibodies against irPNP from potato (anti-StPNP) were used for immunolabelling. Tissue prints revealed that immunoreactants are concentrated in vascular tissues of leaves, petioles and stems. Phloem-associated cells, xylem cells and parenchymatic xylem cells showed the strongest immunoreaction. Immunofluorescent microscopy with fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG supported this finding and, furthermore, revealed strong labelling to stomatal guard cells and the adjacent apoplastic space as well. Biologically active immunoreactants were also detected in xylem exudates of a soft South African perennial forest sage (Plectranthus ciliatus E. Mey ex Benth.) thus strengthening the evidence for a systemic role of the protein. In summary, in situ cellular localization is consistent with physiological responses elicited by irPNPs reported previously and is indicative of a systemic role in plant homeostasis.
2838 related Products with: In situ localization associates biologically active plant natriuretic peptide immuno-analogues with conductive tissue and stomata.Incu Tissue(square vessel Incu Tissue(square vessel Incu Tissue(square vessel Cervix cancer and adjacen Middle advanced stage eso Breast tumor survey tissu FDA Standard Frozen Tissu Liver cancer and normal t Breast infiltrating lobul Rat monoclonal anti mouse Liver carcinoma and norma Colon cancer tissue array
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Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia.To observe expression of CD14 protein and CD14 gene in rat liver sinusoidal endothelial cells (LSECs) during endotoxemia, and the role of CD14 protein in the activation of lipopolysaccharide (LPS)-induced LSECs.
2595 related Products with: Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia.RFP Expressing Human Live GFP Expressing Human Live Human Liver Sinusoidal Mi Octyl â D 1 thioglucopyr Human Large Intestine Mic GFP Expressing Human Inte DNA (cytosine 5) methyltr Human Small Intestine Mic Human Internal Mammary Ar Rabbit Anti-IEX1 Differen OCLN & CSNK1E Protein Pro STK11 & CAB39 Protein Pro
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