Search results for: Rabbit Anti-Human IL-4 Antibodies
#16879875 2006/08/01 To Up
Expression of rabbit interleukin-4 and characterization of its biologic activity on T and B-cells.
The purpose of the current study was to express recombinant rabbit IL-4 (rRbIL-4) and to characterize its biological activity. The cDNA of RbIL-4 was cloned into an insect cell expression vector that allowed for constitutive expression in Sf9 cells and incorporated a 6-histidine tag on the recombinant protein for purification. The purified protein corresponded to the predicted size of rRbIL-4 and was recognized by an anti-human IL-4 antibody in immunoblotting. As shown for IL-4 from other species, a dose-dependent proliferative response was observed in T-lymphoblasts cultured with rRbIL-4. rRbIL-4 also induced increased expression of MHC class II molecules on the surface of rabbit B-cells in a dose-dependent manner. These results indicate that we have produced recombinant rabbit IL-4 that exhibits expected biological activity on rabbit B and T-cells.Brandon T Leader, Wesley C VanVoorhis, Sheila A Lukehart
1079 related Products with: Expression of rabbit interleukin-4 and characterization of its biologic activity on T and B-cells.
1000 TESTS/0.65ml100.00 ul100ug Lyophilized1,000 tests50 1000 tests1000 50 ug 2.5 mg25 mg96T100ug LyophilizedRelated Pathways
#14602230 // To Up
Detection of HLA-G5 secreting cells.
Soluble human leukocyte antigen-G molecules (HLA-G5) can be found in the peripheral blood of healthy females and males, and in other body fluids. To identify cells secreting HLA-G5 we generated a rabbit antiserum against a peptide motif encoded by intron-4 of HLA-G (RaHLA-G/I-4). Utilizing this antiserum as capture and antihuman beta 2-microglobulin as detection reagent, an enzyme-linked immunospot assay specific for HLA-G5 was developed. The results of this enzyme-linked immunospot assay format were proven by the HLA-G1 and soluble HLA-G5 (sHLA-G5) specific mAb MEM/G9 used in parallel as capture reagent. For the choriocarcinoma cell line JEG3 the number of HLA-G5 specific spots was found to be increased after stimulation with IFN-alpha, IFN-beta, and IFN-gamma at various concentrations. In contrast to this, no substantial variation of HLA-G5 specific spots was observed after incubation with lymphokines such as leukemia inhibitory factor, interleukin-10 (IL-10), IL-2, IL-4, and granulocyte-colony-stimulating factor. To clarify the cellular source of secreted HLA-G5 molecules, peripheral blood monocytes, CD4 and CD8 positive T and B cells from healthy donors (n = 14) were tested at a fixed cell number (5000/well) in the absence and presence of IFN-gamma (500 U/ml, 24 hours). In all experiments the number of HLA-G5 specific spots was significantly (p < 0.001) increased primarily in monocytes compared with T and B cells, which suggests that peripheral blood monocytes are the predominant cells secreting HLA-G5.Vera Rebmann, Annika Busemann, Monica Lindemann, Hans Grosse-Wilde
1953 related Products with: Detection of HLA-G5 secreting cells.
1 kit(s) 100tests1.00 flask1 mg4x96 well plate1mg15ml2.00 flaskRelated Pathways
#7681401 // To Up
Identification of two distinct CD5- B cell subsets from human tonsils with different responses to CD40 monoclonal antibody.
This study investigated the response of different CD5- B cell subsets to CD40 monoclonal antibody (mAb) in various combinations with interleukin (IL)-4 or rabbit anti-human mu chain antibody (a-mu-Ab). The different CD5- B cell subsets were isolated from tonsillar B cell suspensions depleted of CD5+ B cells and subsequently fractionated on Percoll density gradients. While resting CD5+ B cells proliferated and produced IgM molecules in response to a-mu-Ab, IL-4 and CD40 mAb as well as to Staphylococcus aureus Cowan strain I (SAC) and IL-2, resting CD5- B cells, which were co-purified in the same 60% Percoll fractions, consistently failed to respond. These cells were, however, activated by the stimuli employed, as demonstrated by their capacity to express the surface activation markers CD69, CD25 and CD71. Resting CD5+ B cells had the typical phenotype of mantle zone B cells (IgM+ IgD+ CD39+ CD38- CD10- CDw75dim), whereas resting CD5- B cells were CD38- CD39- CD10- CDw75 intermediate and expressed surface IgM but relatively little surface IgD and could not be classified as mantle zone or germinal center cells. The finding that purified germinal center cells (CD38+ CD10+ CD39- CDw75bright, IgG+) responded to CD40 mAb and IL-4 and also to SAC plus IL-2 further underlined the differences to resting CD5- B cells. However, some of the data collected suggest possible relationships between CD5- B cells and germinal center cells. The CD5- B cells isolated from the 50% Percoll fraction proliferated in response to a-mu-Ab, CD40 mAb and IL-4 as well as to SAC and IL-2. These cells had the same mantle zone B cell phenotype as the CD5+ B cells, but their capacity to respond to the stimuli in vitro was unrelated to a possible contamination with CD5+ B cells, as documented by the appropriate controls. Furthermore, upon exposure to SAC or phorbol esters, the large majority of CD5- B cells from the 50% Percoll fraction did not express surface CD5 and there was very little if any accumulation of CD5 mRNA. Finally, most of the cycling cells in the stimulated CD5- B cells did not express CD5. The CD5- B cells from the 50% Percoll fraction were comprised of a consistent proportion of cells that expressed surface activation markers.(ABSTRACT TRUNCATED AT 400 WORDS)M Dono, S Zupo, R Masante, G Taborelli, N Chiorazzi, M Ferrarini
2820 related Products with: Identification of two distinct CD5- B cell subsets from human tonsils with different responses to CD40 monoclonal antibody.
1mg500ug100ul100ul1 mg100ul0.1mg100ul100 ug/vial100ulOne 96-Well Microplate Ki 100ulRelated Pathways
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