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Search results for: Rabbit Anti-Visfatin-2(CT) Polyclonal Antibody, Biotin Conjugated

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#33960377   // To Up

Detection of 4 quinolone antibiotics by chemiluminescence based on a novel Nor-Biotin bifunctional ligand and SA-ALP technology.

A simple and effective direct competitive chemiluminescence immunoassay for the detection of 4 kinds of quinolone antibiotics in milk was established using Nor-Biotin (biotin-modified norfloxacin [NOR]) bifunctional ligand and alkaline phosphatase-conjugated streptavidin signal amplification technology. The polyclonal antibody was obtained after the immunization of New Zealand White rabbits using norfloxacin-derived antigen. "Click chemistry" was used for the rapid and facile synthesis of the Nor-Biotin bifunctional ligand. After the optimization of the incubation time and reaction buffer, the direct competitive chemiluminescence assay method was developed and used for sensitive detection of 4 kinds of quinolone drugs (NOR, pefloxacin, ciprofloxacin, and danofloxacin). The IC50 of the 4 kinds of quinolone drugs ranged from 7.35 to 24.27 ng/mL, and the lowest detection limits ranged from 0.05 to 0.16 ng/mL, which were below their maximum residue levels, approved by the EU for treatment of food-producing animals. To demonstrate the applicability of the assay, artificially contaminated milk samples with the 4 quinolone drugs were analyzed. The mean recovery rates of the drugs ranged from 86.31% to 112.11%.
Zhenyu Han, Tieqiang Sun, Zehua Xu, Longxing Fan, Hanxuan Yun, Xuejiao Ge, Xiao Liu, Ying Liu, Bao'an Ning

2396 related Products with: Detection of 4 quinolone antibiotics by chemiluminescence based on a novel Nor-Biotin bifunctional ligand and SA-ALP technology.

50 0.5 mg100 g100ug Lyophilized100ug100ug50 100ug50 0.2 mg50 25 G

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#35518307   2020/05/18 To Up

A signal-enhanced and sensitive lateral flow aptasensor for the rapid detection of PDGF-BB.

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Na Cheng, Yujie Liu, Omar Mukama, Xiaobo Han, Hualin Huang, Shuai Li, Peng Zhou, Xuewen Lu, Zhiyuan Li

1427 related Products with: A signal-enhanced and sensitive lateral flow aptasensor for the rapid detection of PDGF-BB.

100ug Lyophilized100ug Lyophilized50 assays100tests96 tests1 kit100ug Lyophilized100ug Lyophilized100ug Lyophilized 100ul

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#31621104   2019/11/14 To Up

Development of a double-antibody sandwich ELISA for sensitive detection of Yersinia pestis.

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Sang-Yoon Choi, Gi-Eun Rhie, Jun Ho Jeon

1953 related Products with: Development of a double-antibody sandwich ELISA for sensitive detection of Yersinia pestis.

1 kit100ug Lyophilized100ug100ug100ug Lyophilized100ug100ug100ug Lyophilized100ug100ug Lyophilized100ug96 tests

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#30800246   // To Up

Characterization of Monoclonal and Polyclonal Antibodies Recognizing Prostate Specific Antigen: Implication for Design of a Sandwich ELISA.

Prostate cancer is the second most common cancer in men. Prostate-Specific Antigen (PSA) is a tumor-associated glycoprotein with enzymatic activity which is secreted by the prostate gland. Following entry to the blood, 70-90% of PSA forms complexes with protease inhibitors and its enzymatic activity is inhibited. The serum level of PSA is increased and the rate of free PSA (fPSA) to total PSA is decreased in prostate cancer patients. Therefore, measurement of PSA and fPSA in serum is very valuable for diagnosis and prognosis of prostate cancer.
Sahar Raoofi Mohseni, Forough Golsaz-Shirazi, Mostafa Hosseini, Jalal Khoshnoodi, Tannaz Bahadori, Mohammad Ali Judaki, Mahmood Jeddi-Tehrani, Fazel Shokri

2226 related Products with: Characterization of Monoclonal and Polyclonal Antibodies Recognizing Prostate Specific Antigen: Implication for Design of a Sandwich ELISA.

0.2 mg1 kit(96 Wells) 2 ml Ready-to-use 100 6 ml Ready-to-use 0.1 ml25 µg0.2 mg100 ug/vial100.00 ug0.25 mg1 mg

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#27600788   2016/09/04 To Up

Immunoassay for determination of trilobolide.

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Lukáš Huml, Michal Jurášek, Petra Mikšátková, Tomáš Zimmermann, Pavla Tomanová, Miloš Buděšínský, Zdeňka Rottnerová, Markéta Šimková, Juraj Harmatha, Eva Kmoníčková, Oldřich Lapčík, Pavel B Drašar

2599 related Products with: Immunoassay for determination of trilobolide.

100Tests10 ml 125 ml 1 x 961 mg250 mg1x96 well plate100tests 1 G1 ml

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#26248424   // To Up

[Establishment and evaluation of methods for determinating cystic fibrosis transmembrane conductance regulator quantitatively].

To establish and evaluate a BA-ELISA method for the quantitative detection of cystic fibrosis transmembrane conductance regulator (CFTR) protein.
Feng Qiu, Jie Zeng, Kun Li, Ai-jun Chen, Wan-xiang Xu, Ya Ni

1788 related Products with: [Establishment and evaluation of methods for determinating cystic fibrosis transmembrane conductance regulator quantitatively].

0.12 mL 500 ml 50 mg50 250 mg1,000 tests0.1 ml 100ul25 mg0.1 mg5 mg

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#24931549   2014/06/12 To Up

A heterogeneous biotin-streptavidin-amplified enzyme-linked immunosorbent assay for detecting tris(2,3-dibromopropyl) isocyanurate in natural samples.

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Dan Bu, Huisheng Zhuang, Xinchu Zhou, Guangxin Yang

2841 related Products with: A heterogeneous biotin-streptavidin-amplified enzyme-linked immunosorbent assay for detecting tris(2,3-dibromopropyl) isocyanurate in natural samples.

96 samples96 samples96 samples900 tests48 samples96 samples100ug LyophilizedFor 2 miRNA probes, each 100ug100 assays16 Arrays/Slide

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#23833764   2013/07/08 To Up

Electrochemical immunoassay for Salmonella Typhimurium based on magnetically collected Ag-enhanced DNA biobarcode labels.

We describe a sensitive electrochemical immunoassay for Salmonella enterica serovar Typhimurium, a common foodborne pathogen which can cause infection at extremely small doses. The assay is based on the recognition of DNA biobarcode labels by differential pulse anodic stripping voltammetry (DPASV), following Ag enhancement. The biobarcodes consist of latex spheres (mean diameter 506 nm ± 22 nm) modified by ferromagnetic Fe3O4 particles. Each biobarcode is loaded by adsorption with approx. 27 molecules of mouse monoclonal antibody against S. Typhimurium and 3.5 × 10(5) molecules of 12 mer ssDNA. The assay is performed by adding the biobarcode, S. Typhimurium cells, and biotin-conjugated rabbit polyclonal antibody against Salmonella into well plates. After antigen-antibody binding, magnetic collection enables the excess polyclonal antibody to be washed off. Exposure to avidin-coated screen printed electrodes, and formation of the avidin-biotin bond, then enables the excess biobarcode to be removed. The biobarcode remaining on the electrode is quantified by DPASV measurement of Ag(+) ions following catalytic Ag deposition. The assay showed a negligible response to 10(7) CFU mL(-1)E. coli and had a limit of detection of 12 CFU mL(-1) in buffer, and 13 to 26 CFU mL(-1) for heat-killed and whole cell S. Typhimurium in plain milk, green bean sprouts and raw eggs. To the best of our knowledge, this is the lowest reported limit of detection for Salmonella by an electrochemical immunoassay not requiring sample pre-enrichment.
Feby Wijaya Pratiwi, Patsamon Rijiravanich, Mithran Somasundrum, Werasak Surareungchai

2842 related Products with: Electrochemical immunoassay for Salmonella Typhimurium based on magnetically collected Ag-enhanced DNA biobarcode labels.

100ug Lyophilized100ug Lyophilized2 mL100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100 100ug Lyophilized

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#23000004   2012/09/19 To Up

Cysteine-terminated B-domain of Staphylococcus aureus protein A as a scaffold for targeting GABA(A) receptors.

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Nasser M Qtaishat, Hélène A Gussin, David R Pepperberg

1075 related Products with: Cysteine-terminated B-domain of Staphylococcus aureus protein A as a scaffold for targeting GABA(A) receptors.

1000 assays100ug200 100μg100μg100 ml50 ug 0.1ml (1mg/ml)96 Tests10

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#21406139   2011/03/16 To Up

Participation of the 39-kDa glycoprotein (gp39) of the vitelline envelope of Bufo arenarum eggs in sperm-egg interaction.

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Daniel Barrera, Ricardo J Llanos, Dora C Miceli

1355 related Products with: Participation of the 39-kDa glycoprotein (gp39) of the vitelline envelope of Bufo arenarum eggs in sperm-egg interaction.

5 G11 Set1 Set

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