Only in Titles

Search results for: Rabbit Anti-β-Amyloid(1-28) Polyclonal Antibody, Gold conjugated Isotype: IgG

paperclip

#32663533   2020/07/11 To Up

A rapid and sensitive lateral flow immunoassay (LFIA) test for the on-site detection of banana bract mosaic virus in banana plants.

Banana bract mosaic virus (BBrMV) is a serious pathogen threatening the cultivation of banana and plantain worldwide. This study reports the development of a practical, rapid, sensitive, specific and user-friendly lateral flow immunoassay (LFIA) test for the on-site detection of BBrMV. The BBrMV coat protein (CP) was expressed in Escherichia coli and purified and used to immunize rabbits to produce a polyclonal antiserum (anti-BBrMVCP). The test was based on a double-antibody sandwich format. Protein-A affinity column-purified anti-BBrMVCP Immunoglobulins (IgG) (16 μg/mL), conjugated to ∼30 nm gold nanoparticles, was applied onto the conjugate pad. The anti-BBrMVCP IgG and goat anti-rabbit IgG were printed on the surface of a nitrocellulose filter membrane as the test line and control line, respectively. A positive result could be confirmed visually by the presence of a pink band that developed on the LFIA strip within 5-10 min. The detection limit of the test was 10 ng of the expressed recombinant BBrMV CP (rBBrMVCP), and a 1:20 dilution of the BBrMV-infected crude extract. This LFIA test was validated using 114 banana leaf samples randomly collected from the field and the results indicated a very high diagnostic sensitivity (99.04 %) and specificity (100 %) for the test. A Cohen's kappa coefficient of 0.861 obtained also indicated a very good agreement between the LFIA developed in this study and ELISA. This assay could be adopted by farmers, tissue culture industries and quarantine departments for surveys and surveillance. This is the first report on the development of a LFIA-based test for BBrMV detection.
Ramasamy Selvarajan, Prasanya Selvam Kanichelvam, Velusamy Balasubramanian, Sundaram Sethurama Subramanian

1216 related Products with: A rapid and sensitive lateral flow immunoassay (LFIA) test for the on-site detection of banana bract mosaic virus in banana plants.

96 tests100tests1 kit 96 Tests 20 ul100tests25 96 Tests

Related Pathways

paperclip

#32199081   2020/02/24 To Up

Early diagnosis of experimental Trichinella spiralis infection by nano-based enzyme-linked immunosorbent assay (nano-based ELISA).

Trichinellosis is a serious foodborne zoonotic disease. It is an important threat to public health all over the world. Although anti-Trichinella IgG detection is the most widely used method for diagnosis of trichinellosis, but there is an obvious window between clinical symptoms and positive serology. Gold nanoparticles (AuNPs) can be conjugated with antibodies affording them promising applications for bio-chemical detection. Herein, AuNPs-based ELISA was evaluated for the first time in the detection of Trichinella spiralis circulating antigen (CAg) for its potential as a diagnostic tool of experimental infection. Swiss Albino mice were orally inoculated with 100 muscle larvae/mouse. Animals were sacrificed 6, 8, 10, 12, 14, 16, 22 and 28 day-post infection (dpi). Blood samples were tested for CAg by both standard ELISA and nano-based ELISA using anti-rabbit polyclonal IgG conjugated with AuNPs. CAg was only detected by nano-based ELISA 6, 8, 10 dpi and by both formats 12-28 dpi. Nano-based assay recorded a statistically significant high sensitivity (58.33%, 91.67%) and accuracy (72.22%, 94.44%) 8 and 10 dpi, respectively in comparison to standard ELISA. Both assays showed high sensitivity and accuracy 12-28 dpi. Thus, nano-based ELISA could be considered as an early sensitive diagnostic method for experimental trichinellosis.
Maha M Gomaa

2361 related Products with: Early diagnosis of experimental Trichinella spiralis infection by nano-based enzyme-linked immunosorbent assay (nano-based ELISA).

One 96-Well Microplate KiTwo 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate Ki500 testsOne 96-Well Microplate KiOne 96-Well Microplate KiTwo 96-Well Microplate KiOne 96-Well Microplate KiOne 96-Well Microplate Ki

Related Pathways

paperclip

#28727765   2017/07/20 To Up

Defining the target and the effect of imatinib on the filarial c-Abl homologue.

Previously we demonstrated the micro- and macrofilaricidal properties of imatinib in vitro. Here we use electron and multiphoton microscopy to define the target of imatinib in the adult and microfilarial stages of Brugia malayi and assess the effects of pharmacologically relevant levels of imatinib on the adult parasites.
Elise M O'Connell, Olena Kamenyeva, Sara Lustigman, Aaron Bell, Thomas B Nutman

1173 related Products with: Defining the target and the effect of imatinib on the filarial c-Abl homologue.

1 mlmin 2 cartons100.00 ul11200 units500 Units 100 G

Related Pathways

paperclip

#27940044   2016/12/08 To Up

Immunochromatographic detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli with cross-detection of cholera toxin.

Here, we report the development of an immunochromatographic test strip that can detect heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli. Five types of monoclonal antibody (mAb)-producing hybridomas were isolated: three mAbs were A subunit specific and two were B subunit specific. Four mAbs also cross-reacted with both LT proteins derived from swine and human E. coli strains, but only one mAb 57B9 additionally cross-reacted with cholera toxin. Thus, mAb 57B9 was used to form a gold colloid-conjugated antibody for the immunochromatographic test by combination with polyclonal anti-LT rabbit IgG. This test strip detected not only LT in the culture supernatant of LT gene-positive strains, but also cholera toxin in the culture supernatant of Vibrio cholerae. These results indicate that this test strip is suitable for the diagnosis of both enterotoxigenic E. coli and V. cholerae infection.
Hideyuki Arimitsu, Keiko Sasaki, Takao Tsuji

1659 related Products with: Immunochromatographic detection of the heat-labile enterotoxin of enterotoxigenic Escherichia coli with cross-detection of cholera toxin.

1 mg1 mg0.2 mg0.2 mg 5 G2x384 well plate0.1 mg100 U400 assays

Related Pathways

paperclip

#19699744   2009/08/21 To Up

Differences in usability of rabbit IgG and chicken IgY after clean-up and impact on gold labelling properties.

For the application of antibodies in rapid test systems such as Lateral Flow Devices (LFD) antibodies have to be coupled to coloured particles for immediate readability of the test system. In this work colloidal gold was selected for conjugation to the antibodies. Polyclonal rabbit antibodies were chosen for the development of Lateral Flow Devices for the detection of bovine alpha-casein. For antibody comparison chicken egg yolk IgY and sheep IgG were additionally used. Rabbit and chicken antibodies were purified from rabbit sera and egg yolk using affinity chromatography and alternatively ammonium sulphate precipitation, Sheep IgG was commercially obtained. In the course of colloidal gold sol titration experiments differences not only between antibody species but also between differently purified rabbit IgG were observed. While affinity purified rabbit IgG was not able to stabilise colloidal gold particles, antibodies obtained by ammonium sulphate precipitation resulted in a stable gold conjugate suitable for application in Lateral Flow Assays. This work compares and discusses the impact of antibody pre-treatment on further conjugation capacity.
Judith Rudolf, Manuela Führer, Brigitte Galler, Parisa Ansari, Christoph Hasenhindl, Sabine Baumgartner

1158 related Products with: Differences in usability of rabbit IgG and chicken IgY after clean-up and impact on gold labelling properties.

100ul100ug Lyophilized50 ug 1000 100ug Lyophilized1 ml1000 TESTS/0.65ml50 ug 100ug Lyophilized100.00 ul100ug50 ug

Related Pathways

paperclip

#10583677   // To Up

Production and utilization of polyclonal antibodies against nisin in an ELISA and for immuno-location of nisin in producing and sensitive bacterial strains.

Specific nisin polyclonal antibodies (PAb) were produced in rabbits using nisin Z produced by Lactococcus lactis subsp. lactis biovar diacetylactis UL 719. Antisera were obtained from white female New Zealand rabbits that were first immunized with a nisin Z-keyhole limpet haemocyanin conjugate and boosted with free nisin Z. Nisin-specific PAb were purified by affinity chromatography with a yield of 15 mg specific antinisin 100 ml-1 serum. The detection limit of the ELISA test for nisin Z was 0.75 ng ml-1 in buffer but was 1.7 and 3.5 ng ml-1 in milk and complex media broth spiked (5, 10, 20 microg ml-1) with nisin Z, respectively. In nisin Z-spiked samples, the average concentration was between 90 and 107% of actual added amount. In contrast, when the bioassay (microtitration method) was used, only 50-63% of nisin Z biological activity could be detected. In addition, the affinity-purified nisin PAb, antirabbit IgG gold conjugate and transmission electron microscopy were successfully used to locate nisin Z on producing cells and to observe its bactericidal effects against sensitive cells.
M Bouksaim, C Lacroix, R Bazin, R E Simard

2442 related Products with: Production and utilization of polyclonal antibodies against nisin in an ELISA and for immuno-location of nisin in producing and sensitive bacterial strains.

100ug Lyophilized50 ug 50 ug 96T50 ug 100ug Lyophilized100ug Lyophilized96 wells (1 kit)1000 TESTS/0.65ml0.1 mg1 ml0.1ml (1mg/ml)

Related Pathways