Only in Titles

           Search results for: Rabbit anti Progesterone Receptor (pSer190)   

paperclip

#28489882   2017/05/10 Save this To Up

Preparation of a novel antiserum to aromatase with high affinity and specificity: Its clinicopathological significance on breast cancer tissue.

Aromatase inhibitors have been widely used for the endocrine treatment of estrogen-dependent breast cancer in postmenopausal patients. However, clinicopathological studies of aromatase have been limited due to unsatisfactory specificity and/or restricted availability of anti-aromatase antibodies. Here, we have generated a polyclonal antiserum with high affinity and specificity for human aromatase using a monoclonal antibody tagged immunoaffinity chromatography on an industrial production scale. Our preliminary immunohistochemical analysis of 221 invasive breast cancer cases indicated that 87.3% (193/221) had at least 5% aromatase positive cells. The histoscore for aromatase was inversely correlated with pT (p = 0.019), pN (p = 0.001), stage (p < 0.001), histologic grade (p = 0.003), lymphatic infiltration (p < 0.001), venous infiltration (p < 0.001), and Ki-67 index (p < 0.001). However, cancer aromatase expression was independent of estrogen receptor (ER), progesterone receptor (PgR), and human epidermal growth factor receptor 2 statuses. This antiserum will be applicable to clinicopathological examination of aromatase in addition to ER and PgR for an appropriate use of aromatase inhibitor on the treatment of breast cancer. Further studies on the relationship between Aromatase inhibitors have been widely used for the endocrine treatment of estrogen-dependent breast cancer in postmenopausal patients. However, clinicopathological studies of aromatase have been limited due to unsatisfactory specificity and/or restricted availability of anti-aromatase antibodies. Here, we have generated a polyclonal antiserum with high affinity and specificity for human aromatase using a monoclonal antibody tagged immunoaffinity chromatography on an industrial production scale. Our preliminary immunohistochemical analysis of 221 invasive breast cancer cases indicated that 87.3% (193/221) had at least 5% aromatase positive cells. The histoscore for aromatase was inversely correlated with pT (p = 0.019), pN (p = 0.001), stage (p < 0.001), histologic grade (p = 0.003), lymphatic infiltration (p < 0.001), venous infiltration (p < 0.001), and Ki-67 index (p < 0.001). However, cancer aromatase expression was independent of estrogen receptor (ER), progesterone receptor (PgR), and human epidermal growth factor receptor 2 statuses. This antiserum will be applicable to clinicopathological examination of aromatase in addition to ER and PgR for an appropriate use of aromatase inhibitor on the treatment of breast cancer. Further studies on the relationship between aromatase expression and aromatase inhibitors are warranted.

1735 related Products with: Preparation of a novel antiserum to aromatase with high affinity and specificity: Its clinicopathological significance on breast cancer tissue.

Breast cancer high densit High density, multiple br High density breast cance High density (188 cases 2 High density (188 cases 2 High density breast cance High density breast cance High density lung, breast High density multiple org High density tissue array Top 4 types of cancer (co Top 4 types of cancer (co

Related Pathways

paperclip

#27611647   2016/09/09 Save this To Up

Immunohistochemical Localization of Luteinizing Hormone Receptor in the Cyclic Gilt Ovary.

Luteinizing hormone receptor (LHR) is a specific membrane receptor on the granulosa and theca cells that bind to luteinizing hormone (LH), resulting in androgen and progesterone production. Hence, the regulation of LHR expression is necessary for follicle maturation, ovulation and corpus luteum formation. We examined the immunolocalization of LHR in cyclic gilt ovaries. The ovaries were obtained from 21 gilts aged 326.0 ± 38.7 days and weighing 154.6 ± 15.7 kg. The ovarian tissues were incubated with rabbit anti-LHR polyclonal antibody. The follicles were categorized as primordial, primary, preantral and antral follicles. Ovarian phase was categorized as either follicular or luteal phases. The immunolocalization of LHR was clearly expressed in primary, preantral and antral follicles. LHR immunostaining was detected in the cytoplasm of granulosa, theca interna and luteal cells. LHR immunostaining was evaluated using imaging software. LHR immunostaining in the theca interna cells in antral follicles was almost twice as intense as that in preantral follicles (65.4% versus 38.3%, P < 0.01). LHR immunostaining was higher in the follicular phase than in the luteal phase (58.6% versus 45.2%, P < 0.05). In conclusion, the expression of LHR in the theca interna cells of antral follicles in the follicular phase was higher than in the luteal phase. The expression of LHR in all types of the follicles indicates that LHR may impact follicular development from the primary follicle stage onwards.

1950 related Products with: Immunohistochemical Localization of Luteinizing Hormone Receptor in the Cyclic Gilt Ovary.

Interferon-a Receptor Typ Mouse Anti-Human Interleu Mouse Anti-Human Thyroid Mouse Anti-Human Thyroid MOUSE ANTI HUMAN LUTEINIZ interleukin 17 receptor C interferon-alpha receptor GLP 1 ELISA Kit, Rat Gluc Glucagon ELISA KIT, Rat G Leptin ELISA Kit, Rat Lep Anti beta3 AR Human, Poly Human integrin aVb3, affi

Related Pathways

paperclip

#26258752   2016/07/07 Save this To Up

Ki-67 Membranous Staining: Biologically Relevant or an Artifact of Multiplexed Immunofluorescent Staining.

In the process of developing a multiplex of 8 common breast cancer biomarkers (Her2/neu, estrogen receptor, progesterone receptor, Ki-67, aldehyde dehydrogenase-1, NaK-ATPase, cytokeratin 8/18, and myosin smooth muscle) on a single formalin-fixed paraffin-embedded slide using a sequential staining, imaging, and dye bleaching technology developed by General Electric Company, membranous Ki-67 staining was observed and colocalized with Her2/neu staining. Using immunohistochemistry as gold standards, we discovered that membranous Ki-67 was an artifact caused by the binding of cyanine 5-conjugated rabbit polyclonal Ki-67 antibody to a secondary cyanine 3-conjugated donkey anti-rabbit antibody which was previously applied and bound to rabbit Her2/neu antibody in our multiplexing experiment. After blocking with rabbit serum, a successful protocol for 8 biomarker multiplexing without cross-reactivity of antibodies from the same species was developed.

1629 related Products with: Ki-67 Membranous Staining: Biologically Relevant or an Artifact of Multiplexed Immunofluorescent Staining.

Ki-67 Antigen Ki-67 Antigen Ki-67 Antigen SensiTek HRP Anti-Mouse SensiTek HRP Anti-Mouse SensiTek Alk-Phos Anti-M SensiTek HRP Anti-Rabbit SensiTek HRP Anti-Rabbit SensiTek Alk-Phos Anti-R SensiTek HRP Anti-Polyva SensiTek HRP Anti-Polyva SensiTek Alk-Phos Anti-P

Related Pathways

paperclip

#24287037   2013/11/29 Save this To Up

The Bax/Bcl-2 apoptotic pathway is not responsible for the increase in apoptosis in the RU486-treated rat uterus during early pregnancy.

An increase in apoptotic activity has been observed in both the rabbit and the rat endometria following treatment with RU486. The aim of this study was to assess whether Bax and Bcl-2 signaling, in response to RU486, could be crucial role players mediating apoptosis in the rat uterus during early pregnancy. RU486 is a partial progesterone (P4) and estrogen receptor antagonist, functioning to actively silence P4 receptor gene-associated transcription. Although an increase in apoptosis as a result of RU486 administration has been previously reported in rabbits, the specific apoptotic factors and pathways involved in driving this process have not yet been established. Immunofluorescent techniques were used to determine protein expression levels of both Bax and Bcl-2 in RU486-treated endometria at days 4.5, 5.5 and 6.5 of pregnancy. The Bax/Bcl-2 index was used to determine the overall pro- or anti-apoptotic setting at each day of pregnancy, following RU486 administration. Changes in the Bax and Bcl-2 gene expression levels as a consequence of RU486 administration were evaluated using RT-qPCR. Both the protein and gene expression analyses suggest that RU486 induces a change toward an overall anti-apoptotic signal within the Bax/Bcl-2 pathway. These results suggest that the observed increase in apoptosis following RU486 administration is not driven by a shift in the Bax/Bcl-2 ratio toward cell death, when the P4 and estrogen receptors are partially inactivated by RU486, but is possibly regulated by another apoptotic pathway.

2024 related Products with: The Bax/Bcl-2 apoptotic pathway is not responsible for the increase in apoptosis in the RU486-treated rat uterus during early pregnancy.

MultiGene Gradient therm Thermal Shaker with cooli Rabbit Anti-intestinal FA Rabbit Anti-APIP Apaf1 In Rabbit Anti-APIP Apaf1 In FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Multiple organ tumor tiss

Related Pathways

paperclip

#23779096   2013/07/29 Save this To Up

Activation of the LH receptor up regulates the type 2 adiponectin receptor in human granulosa cells.

Adiponectin is a predominantly adipocyte-derived hormone which influences insulin sensitivity and energy homeostasis through at least two receptors, AdipoR1 and AdipoR2. In animal models, adiponectin may regulate ovarian steroidogenesis, folliculogenesis, and ovulation. The receptors AdipoR1 and AdipoR2 are present in the human ovary, but their regulation is unknown. In these studies, we determined the effects of LH receptor activation on the expression and function of the two adiponectin receptors in human granulosa cells.

2820 related Products with: Activation of the LH receptor up regulates the type 2 adiponectin receptor in human granulosa cells.

Interferon-a Receptor Typ Mouse Anti-Human Interleu Human soluble interleukin Goat Anti-Human Serotonin Rabbit Anti-Human Vasoact Human Tumor Necrosis Fact Rabbit Anti-Human Androge interleukin 17 receptor C Olfactory receptor 2A4 an interferon-alpha receptor Human integrin aVb3, affi Human 4 1BB Receptor20 ug

Related Pathways

paperclip

#23739217   2013/07/17 Save this To Up

BAY 1002670: a novel, highly potent and selective progesterone receptor modulator for gynaecological therapies.

Does the novel progesterone receptor (PR) modulator BAY 1002670, based on its preclinical pharmacological profile, offer a potential novel treatment option for uterine fibroids?

2633 related Products with: BAY 1002670: a novel, highly potent and selective progesterone receptor modulator for gynaecological therapies.

Progesterone Receptor (Ph Androgen Receptor (Phosph Androgen Receptor (Phosph Rabbit Anti-Human Androge Rabbit Anti-Human Androge Progesterone Receptor (Ab Androgen Receptor (Ab 650 Progesterone receptor ant AZD-3514 Mechanisms: Andr IGF-1R Signaling Phospho- Insulin Receptor Phospho- Nuclear Membrane Receptor

Related Pathways

paperclip

#23115015   2014/08/27 Save this To Up

Effect of progesterone receptor status on maspin synthesis via nitric oxide production in neutrophils in human breast cancer.

Although progesterone receptor (PR) status, similarly to estrogen receptor status, is of prognostic importance in breast cancer, the involvement of the PR in breast cancer remains obscure. Studies were conducted to determine the function of the PR in neutrophils in the nitric oxide-induced synthesis of maspin, an anti-breast-cancer protein produced in nonmalignant mammary cells and in neutrophils in the circulation.

1406 related Products with: Effect of progesterone receptor status on maspin synthesis via nitric oxide production in neutrophils in human breast cancer.

Human breast invasive duc Interferon-a Receptor Typ Mouse Anti-Human Interleu anti H inh human blood an interleukin 17 receptor C interferon-alpha receptor Human integrin aVb3, affi Breast cancer tissue arra Breast cancer (IDC) tissu Breast cancer and adjacen Breast cancer tissue arra Breast cancer tissue arra

Related Pathways

paperclip

#22796279   2012/10/02 Save this To Up

Not only dopamine D2 receptors involved in Peony-Glycyrrhiza Decoction, an herbal preparation against antipsychotic-associated hyperprolactinemia.

Clinical studies have demonstrated the effectiveness of an herbal preparation called Peony-Glycyrrhiza Decoction (PGD) in alleviating antipsychotic-induced hyperprolactinemia (hyperPRL). In the present study, we further examined the pharmacological action of PGD on prolactin (PRL) secretion using in vitro and in vivo models, with specific attention to the role of dopaminergic mediators and other sex hormones. Treatment with PGD at 1-5mg/ml significantly suppressed PRL secretion and synthesis in MMQ cells, a model of hyperPRL derived from pituitary adenoma cells. The suppressive effects were completely abolished by pretreatment with 10μM haloperidol, a dopamine D(2) receptor antagonist. Consistent with a D(2)-action, PGD did not affect PRL in rat pituitary lactotropic tumor-derived GH3 cells that lack the D(2) receptor expression but significantly increased the expression of D(2) receptors and dopamine transporters (DAT) in PC12 cells. In a rat model of hyperPRL, produced by repeated injection of the dopamine blocker metoclopramide (MCP), chronic PGD (2.5-10g/kg daily) significantly reduced elevated serum PRL. The reduction in magnitude was similar to that elicited by bromocriptine (BMT), a dopamine D(2) receptor agonist currently used for treatment of hyperPRL. Neither PGD nor BMT altered serum estradiol, but PGD reversed decreased serum progesterone to control level, whereas BMT did not. These results indicate that the anti-hyperPRL effects of PGD are associated not only with D(2) receptor and DAT modulation, but also with a normalization of other sex hormone dysfunction. This experimental evidence supports clinical use of PGD as an effective treatment of antipsychotic-induced hyperPRL.

2336 related Products with: Not only dopamine D2 receptors involved in Peony-Glycyrrhiza Decoction, an herbal preparation against antipsychotic-associated hyperprolactinemia.

Anti 3 DG imidazolone Mon Nuclear Membrane Receptor Goat Anti- Dopamine recep Goat Anti- NOTCH2, (inter Goat Anti- Notch1a (zebra Goat Anti- Notch1b (zebra Rabbit Anti-Dopamine D2 R Rabbit Anti-Dopamine D2 R Rabbit Anti-Dopamine D2 R Rabbit Anti-Dopamine D2 R Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti

Related Pathways

paperclip

#21395466   2011/03/14 Save this To Up

Difference in hormone receptor content in breast cancers from Vietnamese and Swedish women.

The aim of the present study was to compare both estrogen (ER) and progesterone receptor (PgR) content in operable breast cancers from Vietnamese and Swedish patients.

1385 related Products with: Difference in hormone receptor content in breast cancers from Vietnamese and Swedish women.

Interferon-a Receptor Typ Mouse Anti-Human Interleu interleukin 17 receptor C interferon-alpha receptor GLP 1 ELISA Kit, Rat Gluc Glucagon ELISA KIT, Rat G Leptin ELISA Kit, Rat Lep Anti beta3 AR Human, Poly Human integrin aVb3, affi IGF-1R Signaling Phospho- Insulin Receptor Phospho- Nuclear Membrane Receptor

Related Pathways

paperclip

#20096851   2010/04/26 Save this To Up

Progesterone receptor expression and proliferative activity in uterine tumours of pet rabbits.

Endometrial adenocarcinoma is the most common uterine tumour of domestic rabbits. The present immunohistochemical study examined the expression of cytokeratin 19 (CK19), the progesterone receptor (PR), the proliferation-associated antigen Ki-67 and telomerase in normal rabbit uterine tissue and examples of endometrial hyperplasia, adenoma and adenocarcinoma. Tubulopapillary adenomas and adenocarcinomas were the most common histological subtypes in this series. Cytoplasmic expression of CK19 was recorded in two of three samples of normal endometrium and in one of three samples of endometrial hyperplasia, in all adenomas and five of six adenocarcinomas. PR was expressed within the nucleus of normal endometrial cells and in one of three samples of endometrial hyperplasia, each of four adenomas and in four of six adenocarcinomas. This finding suggests that PR expression is not directly involved in neoplastic transformation of the endometrium and that such expression is not a prognostic indicator. Nuclear labelling of telomerase activity was found in one of three normal uteri, all samples of endometrial hyperplasia, two of four adenomas, but none of the adenocarcinomas. The proliferation index as determined by Ki-67 expression was 9.7+/-2.75% (mean+/- standard-deviation (SD)) for normal endometrium, 11.29+/-2.5% for hyperplastic endometrium, 19.40+/-3.01% for benign tumours and 19.41+/-7.9% for malignant tumours. These findings may be interpreted to suggest that hormonal and anti-proliferative treatment may be more appropriate for the management of uterine carcinomas in rabbits than anti-telomerase treatment.

2729 related Products with: Progesterone receptor expression and proliferative activity in uterine tumours of pet rabbits.

Progesterone Receptor (P Progesterone Receptor (P Progesterone Receptor (P DNA (cytosine 5) methyltr Progesterone Receptor (Ph Androgen Receptor (Phosph Androgen Receptor (Phosph Interferon-a Receptor Typ Mouse Anti-Human Interleu Rabbit Anti-Human Androge Rabbit Anti-Human Androge Progesterone Receptor (Ab

Related Pathways