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           Search results for: Rat Anti-IAA Monoclonal Antibody, Biotin conjugated, Isotype: IgG   

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#26316179   2015/11/17 Save this To Up

On-bead antibody-small molecule conjugation using high-capacity magnetic beads.

Antibodies labeled with small molecules such as fluorophore, biotin or drugs play an important role in various areas of biological research, drug discovery and diagnostics. However, the majority of current methods for labeling antibodies is solution-based and has several limitations including the need for purified antibodies at high concentrations and multiple buffer exchange steps. In this study, a method (on-bead conjugation) is described that addresses these limitations by combining antibody purification and conjugation in a single workflow. This method uses high capacity-magnetic Protein A or Protein G beads to capture antibodies directly from cell media followed by conjugation with small molecules and elution of conjugated antibodies from the beads. High-capacity magnetic antibody capture beads are key to this method and were developed by combining porous and hydrophilic cellulose beads with oriented immobilization of Protein A and Protein G using HaloTag technology. With a variety of fluorophores it is shown that the on-bead conjugation method is compatible with both thiol- and amine-based chemistry. This method enables simple and rapid processing of multiple samples in parallel with high-efficiency antibody recovery. It is further shown that recovered antibodies are functional and compatible with downstream applications.

2330 related Products with: On-bead antibody-small molecule conjugation using high-capacity magnetic beads.

Protein A Magnetic Beads Protein G Magnetic Beads Protein A G Magnetic Bead Protein L Magnetic Beads Protein A G L Magnetic Be NanoLink™ Amino Magneti NanoLink™ 4FB Magnetic NanoLink™ Streptavidin NanoLink™ Streptavidin NanoLink™ Streptavidin NanoLink™ Streptavidin MagnaLink™ Streptavidin

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#16199376   2005/10/03 Save this To Up

Targeting of daunomycin using biotinylated immunoliposomes: pharmacokinetics, tissue distribution and in vitro pharmacological effects.

Biotinylated immunoliposomes were prepared by a non-covalent (biotin-streptavidin) coupling procedure and conjugated to the OX26 monoclonal antibody directed against the rat transferrin receptor. In vitro, these biotinylated immunoliposomes were used to by-pass P-glycoprotein in multidrug-resistant RBE4 brain capillary endothelial cells and thereby to achieve 2- to 3-fold higher intracellular accumulation of liposomal daunomycin as compared to free drug. The extent of cellular uptake of liposomal daunomycin was dose- and time-dependent, was inhibited by competition with unbound OX26 and was associated with a pharmacological (i.e. cytotoxic) effect. Cytotoxic effects of liposomal formulations of daunomycin, in contrast to the free drug, were apparent only after prolonged incubation periods being indicative of a slow intracellular unpacking and release of liposomal daunomycin. Pharmacokinetics and tissue distribution studies in the rat revealed brain accumulation of daunomycin in OX26-immunoliposomes to higher levels as compared to brain uptake of free daunomycin, or daunomycin incorporated within pegylated liposomes or within unspecific IgG(2a) isotype control immunoliposomes. Such OX26-mediated effects were not observed in other tissues such as spleen, liver, muscle or kidney.

1376 related Products with: Targeting of daunomycin using biotinylated immunoliposomes: pharmacokinetics, tissue distribution and in vitro pharmacological effects.

Mouse Anti-Human Interleu Mouse Anti-Human Interleu Alkaline Phospatase (ALP) Incu Tissue(square vessel Incu Tissue(square vessel Incu Tissue(square vessel Cultrex In Vitro Angiogen Human integrin aVb3, affi T-2 Toxin Mycotoxins ELIS Zearalenone Mycotoxins EL Multi organ carcinoma tis Multi organ carcinoma tis

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#2785143   1989/06/02 Save this To Up

An improved method for the detection of IgE antibody of defined specificity by ELISA using rat monoclonal anti-IgE antibody.

An improved method for the detection of IgE antibody of defined specificity by ELISA using rat monoclonal anti-IgE antibodies is described. The innovation consists of coating the plates first by a monoclonal rat anti-murine IgE antibody, adding the sera to this antibody-coated plates and then adding the biotin-conjugated antigen after the sera. The plates are then reacted with streptavidin-peroxidase and developed. This procedure eliminates possible competitions with other isotypes of the same specificity. The method is useful especially to quantitate IgE with defined specificity in the presence of high amounts of isotypes of the same specificity.

2867 related Products with: An improved method for the detection of IgE antibody of defined specificity by ELISA using rat monoclonal anti-IgE antibody.

MOUSE ANTI BOVINE ROTAVIR MOUSE ANTI BORRELIA BURGD RABBIT ANTI GSK3 BETA (pS Rat Anti-IAA Monoclonal A MOUSE ANTI CANINE DISTEMP MOUSE ANTI HUMAN CD15, Pr MOUSE ANTI HUMAN CD19 RPE MOUSE ANTI HUMAN CD15, Pr MOUSE ANTI APAAP COMPLEX, anti CD16 monoclonal anti anti CD20 monoclonal anti Human IgE antibody, Monoc

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