Only in Titles

           Search results for: Rat Red Blood Cells 10ml   

paperclip

#24751337   2015/04/20 Save this To Up

Evaluation of the repeated-dose liver micronucleus assay using N-nitrosomorpholine in young adult rats: report on collaborative study by the Collaborative Study Group for the Micronucleus Test (CSGMT)/Japanese Environmental Mutagen Society (JEMS)-Mammalian Mutagenicity Study (MMS) Group.

The present study was conducted to evaluate the suitability of a repeated-dose liver micronucleus (LMN) assay in young adult rats as a collaborative study by the Mammalian mutagenicity study (MMS) group. All procedures were performed in accordance with the standard protocols of the MMS Group. Six-week-old male Crl:CD(SD) rats (5 animals/group) received oral doses of the hepatocarcinogen N-nitrosomorpholine (NMOR) at 0 (control), 5, 10, and 30mg/kg/day (10mL/kg) for 14 days. Control animals received vehicle (water). Hepatocytes were collected from the liver 24h after the last dose, and the number of micronucleated hepatocytes (MNHEPs) was determined by microscopy. The number of micronucleated immature erythrocytes (MNIMEs) in the femoral bone marrow was also determined. The liver was examined using histopathologic methods after formalin fixation. The results showed statistically significant and dose-dependent increases in the number of MNHEPs in the liver at doses of 10mg/kg and greater when compared with the vehicle control. However, no significant increase was noted in the number of MNIMEs in the bone marrow at doses of up to 30mg/kg. Histopathology of the liver revealed hypertrophy and single cell necrosis of hepatocytes at doses of 5mg/kg and above. These results showed that the induction of micronuclei by NMOR was detected by the repeated-dose LMN assay, but not by the repeated-dose bone marrow micronucleus assay.

1507 related Products with: Evaluation of the repeated-dose liver micronucleus assay using N-nitrosomorpholine in young adult rats: report on collaborative study by the Collaborative Study Group for the Micronucleus Test (CSGMT)/Japanese Environmental Mutagen Society (JEMS)-Mammalian Mutagenicity Study (MMS) Group.

Multiple organ tumor tiss Alkaline Phospatase (ALP) ELISA TEK™ MBM Thermal Thermal Shaker with cooli FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Multiple organ cancer tis MultiGene Gradient therm

Related Pathways

paperclip

#18472330   2008/05/23 Save this To Up

Injection of Mesobuthus tamulus venom in distal segment of femoral artery evokes hyperventilatory and hypertensive responses in anaesthetised rats.

Intra-arterial (i.a.) injection of Indian red scorpion (Mesobuthus tamulus; BT) venom produces cardiorespiratory changes by involving perivascular receptors. The afferents involved in mediating these reflex responses are not known. The present investigation was conducted to examine the afferents mediating the vasosensory reflexes evoked by i.a. injection of BT venom in the peripheral end of femoral artery in urethane anaesthetised rats. Blood pressure (BP), ECG (for heart rate), and respiratory movements (for rate and depth) were recorded for 30min after the i.a. injection of venom. Minute ventilation (MV) was computed by using appropriate calibrations for depth and rate of respiration. After the injection of venom, there was immediate hyperventilatory, intermediate hypertensive and delayed bradycardiac response. Equal volume of saline (0.10ml, i.a.) did not produce any cardiorespiratory changes thus, eliminating the possibility of stretch mediated responses. Sectioning of ipsilateral sciatic and femoral nerves attenuated the hyperventilatory and hypertensive responses produced by venom significantly. After the neurotomy, the latency of bradycardiac response was shortened significantly. Even the time to reach the peak bradycardiac response was also shortened. The data provide evidences for the partial involvement of somatic nerves in mediating the vascular reflexes.

2620 related Products with: Injection of Mesobuthus tamulus venom in distal segment of femoral artery evokes hyperventilatory and hypertensive responses in anaesthetised rats.

Human Internal Mammary Ar GFP Expressing Human Inte Pressure Injection Cell, Pressure Injection Cell w Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s Sterile filtered rat ser ING1B antisense ING1B sense

Related Pathways