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Search results for: Rat monoclonal anti mouse CCL-20 Anti-Mouse antibodies

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#37218898   2023/04/28 To Up

Identification of the Binding Epitope of an Anti-Mouse CCR6 Monoclonal Antibody (CMab-13) Using 1× Alanine Scanning.

CC chemokine receptor 6 (CCR6) is one of the members of the G-protein-coupled receptor (GPCR) family that is upregulated in many immune-related cells, such as B lymphocytes, effector and memory T cells, regulatory T cells, and immature dendritic cells. The coordination between CCR6 and its ligand CC motif chemokine ligand 20 (CCL20) is deeply involved in the pathogenesis of various diseases, such as cancer, psoriasis, and autoimmune diseases. Thus, CCR6 is an attractive target for therapy and is being investigated as a diagnostic marker for various diseases. In a previous study, we developed an anti-mouse CCR6 (mCCR6) monoclonal antibody (mAb), CMab-13 (rat IgG, kappa), that was applicable for flow cytometry by immunizing a rat with the N-terminal peptide of mCCR6. In this study, we investigated the binding epitope of CMab-13 using an enzyme-linked immunosorbent assay (ELISA) and the surface plasmon resonance (SPR) method, which were conducted with respect to the synthesized point-mutated-peptides within the 1-20 amino acid region of mCCR6. In the ELISA results, CMab-13 lost its ability to react to the alanine-substituted peptide of mCCR6 at Asp11, thereby identifying Asp11 as the epitope of CMab-13. In our SPR analysis, the dissociation constants () could not be calculated for the G9A and D11A mutants due to the lack of binding. The SPR analysis demonstrated that the CMab-13 epitope comprises Gly9 and Asp11. Taken together, the key binding epitope of CMab-13 was determined to be located around Asp11 on mCCR6. Based on the epitope information, CMab-13 could be useful for further functional analysis of mCCR6 in future studies.
Tomohiro Tanaka, Mayuki Tawara, Hiroyuki Suzuki, Mika K Kaneko, Yukinari Kato

2662 related Products with: Identification of the Binding Epitope of an Anti-Mouse CCR6 Monoclonal Antibody (CMab-13) Using 1× Alanine Scanning.

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#36383115   2022/11/16 To Up

Development of a Novel Anti-Mouse CCR6 Monoclonal Antibody (CMab-13) by N-Terminal Peptide Immunization.

The CC chemokine receptor 6 (CCR6) is a G protein-coupled receptor family member that is highly expressed in B lymphocytes, certain subsets of effector and memory T cells, and immature dendritic cells. CCR6 has only one chemokine ligand, CCL20. The CCL20-CCR6 axis has been recognized as a therapeutic target for autoimmune diseases and tumor. This study developed specific monoclonal antibodies (mAbs) against mouse CCR6 (mCCR6) using the peptide immunization method. The established anti-mCCR6 mAb, CMab-13 (rat IgG, kappa), reacted with mCCR6-overexpressed Chinese hamster ovary-K1 (CHO/mCCR6), and mCCR6-endogenously expressed P388 (mouse lymphoid neoplasma) and J774-1 (mouse macrophage-like) cells in flow cytometry. The dissociation constant () of CMab-13 for CHO/mCCR6 cells was determined to be 2.8 × 10 M, indicating that CMab-13 binds to mCCR6 with high affinity. In summary, CMab-13 is useful for detecting mCCR6-expressing cells through flow cytometry.
Teizo Asano, Tomohiro Tanaka, Hiroyuki Suzuki, Guanjie Li, Ren Nanamiya, Nami Tateyama, Yu Isoda, Yuki Okada, Hiyori Kobayashi, Takeo Yoshikawa, Mika K Kaneko, Yukinari Kato

2532 related Products with: Development of a Novel Anti-Mouse CCR6 Monoclonal Antibody (CMab-13) by N-Terminal Peptide Immunization.

100ug100μl0.1 mg100ug Lyophilized100ug0.25 mg100ug Lyophilized100ul100ug100ul100ug Lyophilized100ul

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