Search results for: Rat monoclonal anti mouse CCL-5 Anti-Mouse antibodies
#36546900 2022/12/02 To Up
Epitope Mapping of Anti-Mouse CCR3 Monoclonal Antibodies Using Flow Cytometry.
The CC chemokine receptor 3 (CCR3) is a receptor for CC chemokines, including CCL5/RANTES, CCL7/MCP-3, and CCL11/eotaxin. CCR3 is expressed on the surface of eosinophils, basophils, a subset of Th2 lymphocytes, mast cells, and airway epithelial cells. CCR3 and its ligands are involved in airway hyperresponsiveness in allergic asthma, ocular allergies, and cancers. Therefore, CCR3 is an attractive target for those therapies. Previously, anti-mouse CCR3 (mCCR3) monoclonal antibodies (mAbs), CMab-3 (rat IgG, kappa), and CMab-4 (rat IgG, kappa) were developed using the Cell-Based Immunization and Screening (CBIS) method. In this study, the binding epitope of these mAbs was investigated using flow cytometry. A CCR3 extracellular domain-substituted mutant analysis showed that CMab-3, CMab-4, and a commercially available mAb (J073E5) recognized the N-terminal region (amino acids 1-38) of mCCR3. Next, alanine scanning was conducted in the N-terminal region. The results revealed that the Ala2, Phe3, Asn4, and Thr5 of mCCR3 are involved in CMab-3 binding, whereas Ala2, Phe3, and Thr5 are essential to CMab-4 binding, and Ala2 and Phe3 are crucial to J073E5 binding. These results reveal the involvement of the N-terminus of mCCR3 in the recognition of CMab-3, CMab-4, and J073E5.Nami Tateyama, Teizo Asano, Hiroyuki Suzuki, Guanjie Li, Takeo Yoshikawa, Tomohiro Tanaka, Mika K Kaneko, Yukinari Kato
2995 related Products with: Epitope Mapping of Anti-Mouse CCR3 Monoclonal Antibodies Using Flow Cytometry.
100.00 ug100.00 ug100.00 ug100.00 ug100.00 ug0.5 mg100.00 ug100.00 ug100.00 ug100.00 ug0.1ml (1mg/ml)100.00 ugRelated Pathways
#21606075 2011/05/23 To Up
CC chemokine CCL5 plays a central role impacting infarct size and post-infarction heart failure in mice.
The chemokine CCL5 plays a critical role as neutrophil and macrophage activator do in atherosclerosis and myocardial infarction. Thus, we investigated whether the treatment with a neutralizing monoclonal antibody (mAb) to mouse CCL5 would provide therapeutic benefit when provoking a coronary-associated ischaemic event.Fabrizio Montecucco, Vincent Braunersreuther, Sébastien Lenglet, Benedicte M A Delattre, Graziano Pelli, Vanessa Buatois, Florence Guilhot, Katia Galan, Nicolas Vuilleumier, Walter Ferlin, Nicolas Fischer, Jean-Paul Vallée, Marie Kosco-Vilbois, François Mach
2932 related Products with: CC chemokine CCL5 plays a central role impacting infarct size and post-infarction heart failure in mice.
100 UG 50 UG 50 UG1 Set4/120 Packing /sleeve/bo0.1 mg1 Set100 μg1 Set50 ugRelated Pathways
#20478301 2010/05/16 To Up
Anti-IL-6-receptor antibody promotes repair of spinal cord injury by inducing microglia-dominant inflammation.
We previously reported the beneficial effect of administering an anti-mouse IL-6 receptor antibody (MR16-1) immediately after spinal cord injury (SCI). The purpose of our present study was to clarify the mechanism underlying how MR16-1 improves motor function after SCI. Quantitative analyses of inflammatory cells using flow cytometry, and immunohistochemistry with bone marrow-chimeric mice generated by transplanting genetically marked purified hematopoietic stem cells, revealed that MR16-1 dramatically switched the central player in the post-traumatic inflammation, from hematogenous macrophages to resident microglia. This change was accompanied by alterations in the expression of relevant cytokines within the injured spinal cord; the expression of recruiting chemokines including CCL2, CCL5, and CXCL10 was decreased, while that of Granulocyte/Macrophage-Colony Stimulating Factor (GM-CSF), a known mitogen for microglia, was increased. We also showed that the resident microglia expressed higher levels of phagocytic markers than the hematogenous macrophages. Consistent with these findings, we observed significantly decreased tissue damage and reduced levels of myelin debris and Nogo-A, the axonal growth inhibitor, by MR16-1 treatment. Moreover, we observed increased axonal regeneration and/or sprouting in the MR16-1-treated mice. Our findings indicate that the functional improvement elicited by MR16-1 involves microglial functions, and provide new insights into the role of IL-6 signaling in the pathology of SCI.Masahiko Mukaino, Masaya Nakamura, Osamu Yamada, Seiji Okada, Satoru Morikawa, Francois Renault-Mihara, Akio Iwanami, Takeshi Ikegami, Yoshiyuki Ohsugi, Osahiko Tsuji, Hiroyuki Katoh, Yumi Matsuzaki, Yoshiaki Toyama, Meigen Liu, Hideyuki Okano
1372 related Products with: Anti-IL-6-receptor antibody promotes repair of spinal cord injury by inducing microglia-dominant inflammation.
100ug Lyophilized100ug Lyophilized100ug100ug Lyophilized100ug100ug Lyophilized100ul100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug100ug LyophilizedRelated Pathways
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