Search results for: Recombinant Human CEBPG Proteins
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#20188772 2010/02/25 To Up
Identification of cellular genes critical to recombinant protein production using a Gaussia luciferase-based siRNA screening system.
Development of high-throughput functional genomic screening, including siRNA screening, provides a novel approach for quick identification of critical factors involved in biological processes. Here, we apply this strategy to search for cellular genes involved in recombinant protein production. Since most of biopharmaceutical proteins are secreted proteins, we develop a cell-based reporter assay using a secreted luciferase, Gaussia luciferase (Gluc), as the reporter. Human embryonic kidney 293 (HEK293) cells transiently transfected with the Gluc reporter plasmid are used to screen our siRNA panel. Three cellular genes, CCAAT/enhancer binding protein gamma (CEBPG), potassium channel tetramerisation domain containing 2 (KCTD2), transmembrane protein 183A (TMEM183A), were isolated from the screening. Production of erythropoietin (EPO) was significantly inhibited when CEBPG, KCTD2, and TMEM183A were knocked down. Furthermore, overexpression of CEBPG is shown to significantly improve production of recombinant EPO, interferon gamma, and monoclonal antibody in HEK293 and Chinese hamster ovary cells. Collectively, this novel Gluc-based siRNA screening system is proven to be a useful tool for investigation of secreted protein production in mammalian cells.Teng Rhui Lwa, Chuan Hao Tan, Qiao Jing Lew, Kai Ling Chu, Janice Tan, Yih Yean Lee, Sheng-Hao Chao
1237 related Products with: Identification of cellular genes critical to recombinant protein production using a Gaussia luciferase-based siRNA screening system.
100 51 mg1mg1055101mg51mgRelated Pathways
#19376136 2009/04/17 To Up
Fasting induced up-regulation of activating transcription factor 5 in mouse liver.
Food deprivation (fasting) is commonly encountered in the lives of animals and humans. In mammals, adaptive responses predominantly include the induction of hepatic gluconeogenesis, but the regulatory mechanisms remain unclear. Atf5 (activating transcription factor 5) is a transcription factor of the ATF/cAMP response element-binding protein family and is expressed abundantly in human liver. Atf5 has been associated with stress responses, cell differentiation, proliferation, and survival. However, its role in the liver response to in vivo food deprivation has not yet been investigated.Yusuke I Shimizu, Momoko Morita, Asako Ohmi, Shun Aoyagi, Hitomi Ebihara, Daijuro Tonaki, Yoko Horino, Mika Iijima, Hidenori Hirose, Shigeru Takahashi, Yuji Takahashi
1404 related Products with: Fasting induced up-regulation of activating transcription factor 5 in mouse liver.
100.00 ug2ug100 ul10ug1 kit(96 Wells)100.00 ug500ug100.00 ug200ug100.00 ug500ug200ugRelated Pathways
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