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[Expression and Clinical Significance of PD-L1 and MicroRNA-138-5p in Patients with Acute Myeloid Leukemia].

To investigate the expression and clinical significance of PD-L1 and microRNA-138-5p in the peripheral blood mononuclear cells of patients with acute myeloid leukemia.

1600 related Products with: [Expression and Clinical Significance of PD-L1 and MicroRNA-138-5p in Patients with Acute Myeloid Leukemia].

Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon DNA (cytosine 5) methyltr Androgen Receptor (Phosph Androgen Receptor (Phosph Rabbit Anti-Human Androge Rabbit Anti-Human Androge Androgen Receptor (Ab 650 AZD-3514 Mechanisms: Andr 17β-Acetoxy-2α-bromo-5

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Fluorescent S1 nuclease assay utilizing exponential strand displacement amplification.

We herein devise a simple and label-free strategy to determine S1 nuclease activity by exploiting the target-induced inhibition of exponential strand displacement amplification (eSDA). In principle, a DNA probe that is designed to produce a large amount of duplexes through a process of eSDA, is degraded by the catalytic activity of S1 nuclease. This reaction blocks the initiation of eSDA, leading to the quite-reduced fluorescence of a double-stranded DNA specific fluorescent dye, SYBR Green I compared to the one in the absence of S1 nuclease. With this simple but novel approach, the S1 nuclease activity was selectively assayed with the high sensitivity. In addition, this system was successfully demonstrated to possess the capability to screen potential inhibitors against S1 nuclease.

1779 related Products with: Fluorescent S1 nuclease assay utilizing exponential strand displacement amplification.

LSD 1 Fluorescent Assay k MarkerGeneTM Fluorescent MarkerGeneTM Long Wavelen 8 Octadecyloxypyrene 1,3, MarkerGeneTM Fluorescent MarkerGeneTM Fluorescent MarkerGene™ Multiple Dr Rapid Microplate Assay K UltraRainbow Fluorescent E. coli SSB (Single Stran E. coli SSB (Single Stran E. coli SSB (Single Stran

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An exonuclease-assisted fluorescence sensor for assaying alkaline phosphatase based on SYBR Green I.

In this report, we propose a fast, reliable and convenient approach to determine the alkaline phosphatase (ALP) activity based on a label-free fluorescence strategy. Upon catalysis of ALP, dephosphorylated dsDNA hampers the λ exonuclease (λexo) cleavage, shows high affinity to SYBR Green I (SG I), resulting in a strong fluorescence emission peak at 520 nm. In the absence of ALP, the dsDNA with 5'-phosphoryl-termini could be employed as a substrate of λexo. After cleavage, a weak fluorescence emission peaks at 520 nm could be observed. The assay was both selective and sensitive, and the detection limit was found to be as low as 3 U/L. This method was utilized to evaluate NaVO as ALP inhibitor. The method was successfully applied to the determination of the activity of ALP in spiked human serum samples.

1918 related Products with: An exonuclease-assisted fluorescence sensor for assaying alkaline phosphatase based on SYBR Green I.

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Data on the effect of heat and other technical variables on the detection of microRNAs in human serum.

Data are presented on the number and levels of 384 microRNAs (miRNAs) quantified by reverse-transcription real-time quantitative polymerase chain reaction (RT-qPCR) in human serum analyzed under different experimental conditions. The technical variables tested were 1) heating of the serum samples at 60 °C for 120 minutes prior to RNA extraction no heating; 2) RNA extraction using an Exiqon miRCURY RNA Isolation kit for Biofluids a Systems Biosciences SeraMir Exosome RNA Purification kit; 3) miRNA quantitation by RT-qPCR using an Exiqon SYBR Green Human Panel I an Applied Biosystems TaqMan Human microRNA Array A.

1221 related Products with: Data on the effect of heat and other technical variables on the detection of microRNAs in human serum.

Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s Sterile filtered rat ser TCP-1 theta antibody Sour Recombinant Human Oncosta Recombinant Human PKC the Recombinant Human PKC the Recombinant Human PKC the RAP2C, member of RAS onco Oncostatin M, human recom Oncostatin M, human recom

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Differential gene expression changes and their implication on the disease progression in patients with Chronic Myeloid Leukemia.

The molecular mechanisms responsible for disease progression of CML are not conclusive. The main functional changes associated with disease evolution in CML was high proliferation rate, decreased apoptosis, blockade of differentiation, and strong resistance to chemotherapeutic agents. The current study analyzed the relative expressional profiles of genes related with proliferation, apoptosis, differentiation, and resistance to chemotherapeutic agents such as c-MYC, BAD, BCL-2, C/EBPα/-β and ABCB1 respectively in different clinical stages of CML by SYBR Green I quantitative real-time (qRT) PCR. We selected a total of 183 CML patients and 30 healthy control samples. The study populations were classified into four groups, including de novo CML-CP (50/183), CML-AP (32/183), CML-BC (51/183) and Imatinib Mesylate or IM resistant CML-CP (50/183) groups. qRT PCR analysis revealed that significant overexpression of c-MYC, ABCB1 and BCL-2 was observed in advanced phases and IM resistant CP of CML compared to healthy controls. Likewise, the mean expression level of BAD, C/EBPα/-β genes were found to be significantly down regulated. Present study concluded that the complex interplay of several candidate genes like overexpression of c-MYC, ABCB1, BCL-2 and down regulation of BAD, C/EBPα/-β played a significant role in the disease evolution and development of drug resistant in CML.

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DNA (cytosine 5) methyltr Anti 3 DG imidazolone Mon Rectum disease spectrum ( Kidney disease spectrum ( Brain disease spectrum (b Colon disease spectrum (c Ovarian disease spectrum Ovary disease spectrum (o Pancreatic disease spectr Small intestine disease ( Small intestine disease s Human Epstein-Barr Virus

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- and - expression in clinical no response-antimonial isolates.

Cutaneous leishmaniasis (CL) is a major disease in many parts of the world. Since no vaccine has been developed, treatment is the best way to control it. In most areas, antimonial resistance whose mechanisms have not been completely understood has been reported. The main aim of this study is gene expression assessing of - and - in clinical isolates. The patients with CL from central and north Iran were considered for this study. The samples were transferred in RNAlater solution and stored in - 20 °C. RNA extraction and cDNA synthesis were performed. The gene expression analysis was done with SYBR Green real-time PCR using ∆∆CT. Written informed consent forms were filled out by patients, and then, information forms were filled out based on the Helsinki Declaration. Statistical analysis was done with SPSS (16.0; SPSS Inc, Chicago) using independent test, Shapiro-Wilk, and Pearson's and Spearman's rank correlation coefficients. ≤ 0.05 was considered significant. The gene expression of and had no relation with sex and age. The gene expression was high in sensitive isolates obtained from north of the country. The gene expression was significant in sensitive and no response-antimonial isolates from the north, but no significant differences were detected in sensitive and resistant isolates from central Iran. Differential gene expression of and in various clinical resistances isolates in different geographical areas shows multifactorial ways of developing resistance in different isolates.

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Multiple organs tumor and Stomach adenocarcinoma wi Liver carcinoma and norma Liver carcinoma and norma Liver carcinoma and norma Lung carcinoma and normal Lung carcinoma and normal Lung cancer tissue array Lung squamous cell carcin Lung adenocarcinoma and n Colon cancer and normal t Colon cancer and normal t

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Development and comparison of real-time and conventional PCR tools targeting β-tubulin gene for detection of infection in silkworms.

Microsporidiosis (Pebrine) caused by the microsporidian parasite is one of the important devastating disease which affect the silk production leading to an unprofitable harvest. Till date ribosomal RNA (rRNA) gene was used as a target for detection of microsporidian species. In this study, we describe conventional and SYBR green based real-time PCR techniques alternatively targeting β-tubulin gene for quantitative detection of microsporidia infecting both the mulberry and non-mulberry silkworms. The modified DNA extraction method followed in our study was found to be easy, economical and could be used for both conventional and real time PCR as template. The real time qPCR revealed the expression of β-tubulin gene in different infected tissues of the silkworm . The sensitivity of the SYBR green based real time PCR was found to be 100 times more than the conventional PCR and PCR was found more sensitive than the microscopic examination. The developed method did not produce any false positive results with the other silkworm pathogens and healthy silkworm. The data suggest that both the developed PCR methods targeting β-tubulin gene could be used effectively in quarantine process at seed centres for early detection of microsporidian infection in silkworms.

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DNA (cytosine 5) methyltr Dengue Virus General type Avian Influenza virus H5N Avian Influenza Virus H5N Avian Influenza virus H5N Avian Influenza virus H5N Influenza virus A&B Real Syringe pump can be contr Leukemia m BCR Fusion Gen Human Epstein-Barr Virus Androgen Receptor (Phosph Androgen Receptor (Phosph

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Zero background and triple-signal amplified fluorescence aptasensor for antibiotics detection in foods.

It's important to eliminate matrix interference for accurate detecting antibiotic residues in complex food samples. In this study, we designed a zero-backgrounded fluorescence aptasensor to achieve on-site detection of antibiotic residues, with chloramphenicol (CAP) as representative analyte. Moreover, a three stir-bars assisted target recycling system (TSBTR) was designed to achieve triple signal amplification and increase the sensitivity. The bars included one magnetic stir-bar modified with two kinds of long DNA chains, and two gold stir-bars modified with Y shape-duplex DNA probes respectively. In the presence of CAP, the target could recurrently react with the probes on the bars and replace a large amount of long DNA chains into supernatant. After then, the bars were taken out and SYBR green dye was added to the solution. The dye can specifically intercalate into the duplex structures of DNA chains to emit fluorescence while not emitting a signal in its free state. Under the optimized experimental conditions, a wide linear response range of 5 orders of magnitude from 0.001 ng mL to 10 ng mL was achieved with a detection limit of 0.033 pg mL CAP. The assay was successfully employed to detect CAP in food samples (milk & fish) with consistent results with ELISA's. High selectivity and sensitivity were attributed to the zero background signal and triple signal-amplification strategy. Moreover, the detection time can be shortened to 40 min due to that three signal amplified process can occur simultaneously. The fluorescent aptasensor was also label- and enzyme-free. All these ensure the platform to be rapid, cost-effective, easily-used, and is especially appropriate for detection antibiotics in food safety.

2014 related Products with: Zero background and triple-signal amplified fluorescence aptasensor for antibiotics detection in foods.

Cell Meter™ Apoptotic a Amplite™ Fluorimetric F Amplite™ Fluorimetric H Amplite™ Intracellular Amplite™ Fluorimetric P Amplite™ Fluorimetric A FluoroQuest™ Fluorescen Cell Meter™ Fluorimetri Cell Meter™ Fluorimetri Signal transduction antib AKT PKB Signaling Phospho AMPK Signaling Phospho-Sp

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Identification of a novel tRNA-derived RNA fragment exhibiting high prognostic potential in chronic lymphocytic leukemia.

tRNA-derived fragments (tRFs) are 16-28 nucleotide fragments, which occur by specific cleavage of tRNA molecules. i-tRF-GlyGCC is an internal tRF, originating from the tRNAs bearing the Glycine anticodon "GCC". Other tRFs generated from tRNA have proved to be associated with various cancer types. Chronic lymphocytic leukemia (CLL) is a type of leukemia during which CD5+ B lymphocytes accumulate in marrow and lymphoid tissues and is characterized by differential clinical features among patients. In this original study, we verified the existence of this tRF experimentally, and examined, for the first time, the putative utility of this molecule as a prognostic biomarker in CLL, by developing an innovative tRF quantification method, based on the SYBR chemistry. Total RNA was extracted from peripheral blood mononuclear cells (PBMCs) collected from 91 CLL patients and 43 non-leukemic blood donors. In vitro polyadenylation and reverse transcription using an oligo-dT-adapter were carried out and quantitative expression analysis of i-tRF-GlyGCC was performed by an in-house-developed real-time quantitative PCR assay. Advanced biostatistical analysis followed, to assess our results. As revealed by Kaplan-Meier survival analysis, elevated levels of i-tRF-GlyGCC were associated with poor overall survival of CLL patients (p<0.001). Univariate bootstrap Cox regression analysis confirmed these results, by demonstrating a higher hazard ratio (HR) of 3.40 (95% CI=1.6-7.18, p=0.001) for patients overexpressing for i-tRF-GlyGCC, compared to patients with lower expression levels. Multivariate bootstrap Cox regression analysis revealed that the prognostic value of this tRF is independent of clinical stage, IGHV mutational status and CD38 expression (Binet or Rai stage: p<0.001. risk group, p=0.001; bootstrapping p=0.003 and p=0.004 respectively). Our findings demonstrate that the expression status of i-tRF-GlyGCC could serve as potential indicator of unfavorable prognosis in this hematological malignancy, as its prognostic significance is independent of other established prognostic factors in CLL.

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The influence of probiotic diet and chondroitin sulfate administration on Ptgs2, Tgfb1 and Col2a1 expression in rat knee cartilage during monoiodoacetate-induced osteoarthritis.

Osteoarthritis (OA) is a common worldwide disease induced by a wide range of biochemical processes, mainly inflammation and degradation of collagen. The aim of this study, was to describe the effect of a multistrain probiotic (PB) and chondroitin sulfate (CS), administered separately or in combination, on the expression of Ptgs2, Tgfb1 and Col2a1 during monoiodoacetate-induced OA in male rats.

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CAR,Car,Constitutive andr Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon DNA (cytosine 5) methyltr Human Epstein-Barr Virus Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Androgen Receptor (Phosph

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