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Search results for: SensiTek HRP Anti-Mouse (DAB) Staining System

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#35096347   2021/10/31 To Up

Evaluation of New Immunohistochemical Approaches for the Study of Kidney Tumors in Geriatric.

Kidney malignancies are among the most deadly genitourinary tumors. It is more common in males and is often seen in people aged 60-70 years old. The incidence rate of kidney cancer seems to be increasing. One reason for this may be the fact that imaging techniques, such as computed tomography scans are more commonly used. These tests may lead to the accidental detection of more kidney cancers. Fortunately, kidney cancer is often detected in the early stages, when the tumor is small and confined to the kidney. The objective of this study was the development of new diagnostic immunohistochemical methods. Clinical examination material of 134 people, including 94 (70%) males and 40 (30%) females, were used in this study. Immunohistochemical staining of tryptase was carried out in compliance with the requirements using Anti-Mast Cell Tryptase antibodies. Goat anti-mouse antibodies #AS-M1-HRP were used as secondary antibodies, visualized with ImmPACTTM DAB Peroxidase Substrate Kit (#SK-4105) according to the instructions of the manufacturer. The nuclei were counterstained with Mayer's hematoxylin, and the sections were embedded in a permanent mounting medium. The immunohistochemical study showed an increase in both tryptase- and chymase-positive mast cells in the renal parenchyma, compared with the control group. The number of mast cells with tryptase expression directly in the tumor was significantly less than the peritumoral localization. A similar pattern was observed for chymase-positive mast cells as the content of the tumor was more than 10 times higher than the intratumoral arrangement. The histological and immunological characteristics did not differ in different age groups. The immunohistochemical method of research in the diagnosis of renal tumors plays an important diagnostic and prognostic value. It can assist pathologists in difficult and ambiguous cases to correctly diagnose renal tumors. This will make it possible to prescribe the correct treatment and predict the course of malignant tumor growth in patients.
T V Pavlova, N B Pilkevich, D V Bessmertnyi, I A Pavlov, D V Atiakshin, L A Pavlova

1030 related Products with: Evaluation of New Immunohistochemical Approaches for the Study of Kidney Tumors in Geriatric.

96T500 MG

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#20458863   // To Up

Detection of the bovine viral diarrhoea/mucosal disease (BVD/MD) virus in tissues from aborted ruminant foetuses using immunohistochemistry.

Various tissues from aborted ruminant foetuses were collected, fixed in formalin and embedded in paraffin wax. Sections were made and exposed to a primary monoclonal antibody against the bovine viral diarrhoea/mucosal disease (BVD/MD) virus, and subsequently to a goat anti-mouse secondary antibody conjugated to horse radish peroxidase (HRP). Diaminobenzidine (DAB) was the substrate and it released a brown pigment in the tissues on reacting with the HRP in an immunohistochemistry (IHC) procedure. Of 27 aborted foetuses, an immunoperoxidase staining reaction was observed in 1 ovine and 5 bovine foetuses. The IHC procedure located BVD/MD viral antigen in a wide variety of foetal tissues including cerebral cortical neurons, the pseudostratified columnar epithelial cells lining the bronchi, alveolar lining cells and alveolar macrophages, hepatocytes, renal tubular lining cells and the Purkinje fibres in the myocardium.
S M Njiro, C M Nkosi

1466 related Products with: Detection of the bovine viral diarrhoea/mucosal disease (BVD/MD) virus in tissues from aborted ruminant foetuses using immunohistochemistry.

100 100ug/vial110 500 tests

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#16716219   2006/05/22 To Up

Tissue localization of collagenase and leucine aminopeptidase in the bovine filarial parasite Setaria cervi.

Like other helminth proteases, filarial proteases have also been shown to require for parasite survival inside the host and mediate various physiologic processes such as tissue invasion, feeding, embryogenesis and host immune evasion. Many of these proteases have shown potential for vaccines and chemotherapeutic agents against active filarial infections. Setaria cervi is a bovine filarial parasite and serves as a good parasite model for the studies in lymphatic filariasis. Recently, a 175 kDa collagenase and leucine aminopeptidase (LAP) have been purified and characterized from the bovine filarial parasite S. cervi and shown to be potential vaccine candidate and diagnostic marker, respectively for human lymphatic filariasis. However, their tissue localizations and putative roles in the parasite biology have not yet been examined and thus remain unclear. Therefore, the current study attempts to localize and explore the putative roles of these two enzymes in S. cervi.
Daya R Pokharel, Reeta Rai, Pankaj Kumar, C M Chaturvedi, Sushma Rathaur

1050 related Products with: Tissue localization of collagenase and leucine aminopeptidase in the bovine filarial parasite Setaria cervi.

96T

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#2988598   // To Up

Ultrastructural localization of transferrin, transferrin receptor, and iron-binding sites on human placental and duodenal microvilli.

Ultrastructural methods were used to determine the subcellular location of the transferrin receptor, transferrin and iron-binding sites on human term placenta and human duodenum microvillus surfaces. The transferrin receptor and transferrin were localized by immunocytochemical methods employing either OKT9, a human transferrin receptor monoclonal antibody, or mouse anti-human transferrin (ATfn), both followed by a horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (GAM-HRP) and diaminobenzidine (DAB) sequence. Iron-binding sites were localized by acid ferrocyanide (AF) staining after saturation of tissue specimens with iron, accomplished with iron nitrilotriacetate (FeNTA), a known transferrin iron donor. Placental microvillus surfaces demonstrated staining for the OKT9-GAM-HRP-DAB-reactive transferrin receptor, ATfn-GAM-HRP-DAB-reactive transferrin, and FeNTA-AF-reactive iron acceptor, whereas enterocyte microvillus surfaces lacked significant staining with each of these methods. FeNTA-AF stained iron-binding substance in placental and enterocyte microvilli and cytoplasmic matrix. Thus using the same ultrastructural immunostaining and cytochemical methods transferrin receptor, transferrin, and nitrilotriacetate iron acceptor sites can be demonstrated on the microvillus surface of human placenta but not on the microvillus surface of human duodena.
R T Parmley, J C Barton, M E Conrad

1697 related Products with: Ultrastructural localization of transferrin, transferrin receptor, and iron-binding sites on human placental and duodenal microvilli.

100ul1 ml200 1000 100 100 ug/vial0.1 mg100ul100ug100ug Lyophilized

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#6307335   // To Up

Ultrastructural localization of the transferrin receptor and transferrin on marrow cell surfaces.

The monoclonal antibody OKT9 (a known transferrin receptor antibody) and a monoclonal antibody to transferrin (ATfn) were used to localize the transferrin receptor and transferrin on marrow cells. After incubation of cell suspensions with the antibody, the cells were reacted with an affinity purified Fab fragment of goat anti-mouse IgG conjugated to horseradish peroxidase (GAM-HRP), which was in turn visualized by reaction with 3,3'-diaminobenzidine (DAB). Erythroblast cell surfaces stained intensely with OKT9-GAM-HRP-DAB, weaker staining was observed on reticulocyte surfaces, whereas erythrocyte surfaces lacked staining. Staining was present on surface caveolae, which often contained endogenous ferritin particles. Moderate to strong OKT9 staining was observed on less than 10% of presumed lymphoid cells. Monocytes, macrophages, promyelocytes, granulocytes, megakaryocytes and platelets consistently lacked OKT9 staining. ATfn-GAM-HRP-DAB staining of erythroid cells was similar to that observed with OKT9 staining; however, in contrast to the latter staining, ATfn stained the surfaces of megakaryocytes, platelets, monocytes and most lymphocytes. Promyelocytes stained weakly, whereas late granulocytes lacked staining. These results indicate that the T9 transferrin receptor (1) is largely confined to erythroid cells in marrow, (2) is diffusely distributed on the surface of early erythroid cells, (3) decreases with cell maturation, and (4) is lost when haemoglobin synthesis is completed. Transferrin appears in a similar distribution on erythroid cell surfaces but also appears to bind to some cell surfaces lacking the T9 receptor.
R T Parmley, I Hajdu, F R Denys

2545 related Products with: Ultrastructural localization of the transferrin receptor and transferrin on marrow cell surfaces.

100 ug/vial100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100 100ug Lyophilized100ug Lyophilized100ug Lyophilized100

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