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Effect of Silver Fluoride in Preventing the Formation of Artificial Dentinal Caries Lesions in vitro.

To investigate the effect of silver fluoride in preventing the development of artificial caries lesions in root dentine using an artificial mouth system (AMS).

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Star-Shaped Poly(2-ethyl-2-oxazoline) and Poly(2-isopropyl-2-oxazoline) with Central Thiacalix[4]Arene Fragments: Reduction and Stabilization of Silver Nanoparticles.

The interaction of silver nitrate with star-shaped poly(2-ethyl-2-oxazoline) and poly(2-isopropyl-2-oxazoline) containing central thiacalix[4]arene cores, which proceeds under visible light in aqueous solutions at ambient temperature, was studied. It was found that this process led to the formation of stable colloidal solutions of silver nanoparticles. The kinetics of the formation of the nanoparticles was investigated by the observation of a time-dependent increase in the intensity of the plasmon resonance peak that is related to the nanoparticles and appears in the range of 400 to 700 nm. According to the data of electron and X-ray spectroscopy, scanning and transmission electron microscopy, X-ray diffraction analysis, and dynamic light scattering, the radius of the obtained silver nanoparticles is equal to 30 nm. In addition, the flow birefringence experiments showed that solutions of nanoparticles have high optical shear coefficients.

2174 related Products with: Star-Shaped Poly(2-ethyl-2-oxazoline) and Poly(2-isopropyl-2-oxazoline) with Central Thiacalix[4]Arene Fragments: Reduction and Stabilization of Silver Nanoparticles.

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Preliminary Study of a Single Instillation of Low-Concentration High-Volume Silver Nitrate Solution for Chyluria: Is >10 mL Instillation an Absolute Contraindication in the Real World?

Silver nitrate instillation (SNI) is one form of treatment for chyluria. However, there is the opinion that a high volume of SNI (>10 mL) should be avoided because life-threatening complications have been reported. However, we have noticed that most severe complications occur in high-concentration treatments (≥1%), even with a small volume. In addition, a small volume (≤10 mL) of low-concentration (<1%) SNI occasionally causes recurrence. Based on these facts, we aimed to evaluate a preliminary study of a novel single-injection regimen of low-concentration high-volume SNI. In this preliminary study, nine patients who underwent SNI were retrospectively examined. Patient characteristics, anesthesia, procedure, efficacy, complications, and duration of hospital stay were investigated. The volume of silver nitrate solution was decided based on symptoms, findings of pyelography, and vital signs, and it was given as a single instillation. This study was approved by the Institutional Review Board. The ranges of silver nitrate concentration and volume were 0.1%-0.5% and 15-30 mL, respectively. In all patients, proteinuria and cloudy urine disappeared immediately. However, two patients had recurrence after the initial SNI. These two patients were subsequently treated with increasing concentrations of silver nitrate (0.2% and 0.5%) from 0.1%, and they had complete symptomatic relief without recurrence for more than 6 years. None of the patients experienced severe complications. In conclusion, our preliminary study showed that a single instillation of low-concentration (0.1%-0.5%) and high-volume (15-30 mL) SNI is safe and useful. It is worth considering as a treatment option for chyluria.

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Validation of a method of quantifying 3D leakage in dental restorations.

A reliable and validated protocol for nondestructive analysis of the entire restorative interface that could be applicable in studies assessing the adaptation of direct and indirect restorations is lacking.

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Outcome Of Use Of 1% Silver Nitrate In Patients With Low Lying Perianal Fistula.

Anorectal fistula is a common illness which is seen among the middle-aged male patients. Various surgical procedures have been proposed and are associated with the recurrence of the fistula, repeated surgery and in some cases incontinence which may reach up to 43% in complicated fistulas. The purpose of this study was to assess and formulate a non-surgical procedure for fistula in ano through irrigation of the fistula tract with 1% of silver nitrate solution in healing the low perianal fistula.

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Mass Cytometry Enabling Absolute and Fast Quantification of Silver Nanoparticle Uptake at the Single Cell Level.

In the last decades, significant efforts have been made to investigate possible cytotoxic effects of metallic nanoparticles (NPs). Methodologies enabling precise information regarding uptake and intracellular distribution of NPs at the single cell level remain to be established. Mass cytometry (MC) has been developed for high-dimensional single cell analyses and is a promising tool to quantify NP-cell interactions. Here, we aim to establish a new MC-based quantification procedure to receive absolute numbers of NPs per single cell by using a calibration that considers the specific transmission efficiency (TE) of suspended NPs. The current MC-quantification strategy accept TE values of complementary metal solutions. In this study, we demonstrate the different transmission behavior of 50 nm silver NPs (AgNP) and silver nitrate solution. We have used identical AgNPs for calibration as for -differentiated macrophages (THP-1 cell line) in a time- and dose-dependent manner. Our quantification relies on silver intensities measuring AgNPs in the same detection mode as the cells. Results were comparable with the TE quantification strategy using AgNPs but differed when using ionic silver. Furthermore, intact and digested cell aliquots were measured to investigate the impact of MC sample processing on the amount of AgNPs/cell. Taken together, we have provided a MC-specific calibration procedure to precisely calculate absolute numbers of NPs per single cell. Combined with its unique feature of multiplexing up to 50 parameters, MC provides much more information on the single cell level than single cell-inductively coupled plasma mass spectrometry (SC-ICPMS) and, therefore, offers new opportunities in nanotoxicology.

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A paper-based length of stain analytical device for naked eye (readout-free) detection of cystic fibrosis.

The test of sweat chloride is routinely performed as a worldwide newborn screening (NBS) to the diagnosis of cystic fibrosis (CF) in infants. However, the available methods for measurement of chloride in sweat suffer from such limitations as either low selectivity and/or requiring relatively large sample size. In this work, we have designed an analytical ruler that can measure chloride ion in sweat and hence can be used for the diagnosis of cystic fibrosis. This micro-pad (μ-PAD) device is fabricated by making hydrophilic micro-channel on a filter paper impregnated with silver dichromate. After addition of chloride ion-containing sweat sample, it moves through the channel, leading to the formation of an AgCl sediment, which deposits as a white color stain, the length of which in the channel being proportional to the amount of chloride ion in sweat. A well-defined linear relation was observed between the length of white color stain and the concentration of chloride ion in the sample solutions with a relative standard deviation of 3.6% (n = 3) for an artificial sweat sample containing 100 mM chloride ion. The possible interfering effects of several different cations and anions on the detection of chloride ion were investigated and the results well-confirmed the selectivity of the proposed method. With the use of only 2.0 μL of the sample solution, the μPAD was able to measure the chloride content of sweat over a concentration range of 20.0-100.0 mM, which covers both the healthy range (˂ 40 mM) and the risky range (˃60 mM) of chloride ion. Analysis of chloride content of sweat samples by the μPAD agreed well with those obtained by a standard electrochemical method (with relative errors of lower than 10%).

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Electrospinning of hyaluronan/polyvinyl alcohol in presence of in-situ silver nanoparticles: Preparation and characterization.

Novel nanofibers mats were fabricated by electrospinning of polyvinyl alcohol/hyaluronan (PVA/HA) solutions in the presence of silver nanoparticles (AgNPs). The AgNPs were synthesized by in-situ chemical reduction of silver ions (Ag) using HA as a reducing and stabilizing agent. Narrow size distribution and spherical shape of AgNPs were achieved by optimizing the initial silver nitrate concentration (0.01 to 1 M) and reaction time (10-60 min). HA-AgNPs nanocomposite and PVA/HA-AgNPs nanofibrous mats were fabricated by electrospinning technique from aqueous solution containing a different mass ratio of PVA and HA-AgNPs and characterized by UV/Vis spectroscopy, SEM, TEM, DLS, XRD, TGA, and ATR-FTIR. Mechanical and rheological properties were also investigated and discussed. The novel nanofibrous mats show great potential in skin regeneration and drug carrier applications.

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Assessment of the aerosol distribution pattern of a single-port device for intraperitoneal administration of therapeutic substances.

In the last 20 years, intraperitoneal chemotherapy (IPC) has been explored as a modality for the management of peritoneal metastases of gynecologic, gastrointestinal, and primary peritoneal tumors. Direct delivery of chemotherapeutic agents to the peritoneal cavity space has proved superior to systemic chemotherapy when evaluating characteristics such as drug concentration reached in the peritoneal space, penetration into peritoneal metastases, and chemotherapy-related toxicity. Traditionally, IPC is delivered by peritoneal lavage with a liquid solution. This form of delivery has limitations, including inhomogeneous intraperitoneal distribution and limited ability to penetrate tissues and metastatic nodules. An alternative mode of delivery is so-called pressurized intraperitoneal aerosol chemotherapy (PIPAC). Within this context, the present study sought to identify the pattern of spatial distribution of therapeutic solutions aerosolized into the peritoneal space using a single-port PIPAC device and ascertain whether the aerosolized method is superior to the traditional (liquid) mode of IPC delivery.

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Silver deposition optimization process on ultrananocrystalline diamond applied to nitrate reduction.

Nitrate is a serious contaminant of ground and surface water, which cause high concern in the field of health and environmental protection. In this work, a ternary composite of silver/boron-doped ultrananocrystalline diamond/reticulated vitreous carbon (Ag/B-UNCD/RVC) was prepared and its electrocatalytic activity for nitrate reduction was examined. B-UNCD films were grown by hot filament chemical vapour deposition technique on RVC after a substrate seeding process improvement using 4 nm diamond powder. Compared to conventional 0.25 µm diamond seeding, this new procedure allowed uniform RVC coverage avoiding its etching process during diamond growth, in addition to obtaining more reproductive electrodes. In order to improve the catalytic effect of B-UNCD/RVC electrodes for nitrate reduction, silver deposition was performed on B-UNCD surface after oxidation for 10, 20 and 30 min in 0.5 mol L HSO solution using a potential of 2 V vs. Ag/AgCl. The most oxidized diamond film (30 min of oxidation) presented the highest silver deposit and particle adhesion. Thus the electrochemical response to nitrate of B-UNCD/RVC oxidized for 30 min was compared to that of RVC and B-UNCD/RVC electrodes showing the important Ag influence in the catalytic process.

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