Search results for: Streptavidin [+HRP] Antibody Array
#24974892 2014/07/11 Save this To Up
Orthogonal enzyme arrays on a DNA origami scaffold bearing size-tunable wells.A new waffle-like DNA origami assembly (DNA waffle) with nine nanometer-scale wells in a 3 × 3 matrix pattern has been successfully constructed and used as a scaffold for selective nano-patterning of individual protein molecules. The folding pattern of the scaffold was specially designed so that the dimensions of each well could be independently tuned according to the dimensions of the guest nanoparticles. We demonstrated that two distinct proteins, streptavidin (SA) tetramer (d = 5 nm) and anti-fluorescein antibody (IgG) (inter-paratope distance ∼ 14.0 nm), could be selectively captured in size-variable wells of dimensions 6.8 × 12 × 2.0 nm for SA and 6.8 × 12 × 2.0 nm or 10.2 × 12 × 2.0 nm for IgG, respectively, through the attachment of two biotins or two fluoresceins at the two edges of each well. This allowed the formation of a heterogeneous protein nanoarray of individual molecules. The position of SA or IgG capture can be fully controlled by placement of biotins or fluoresceins in the nanoarray well. Moreover, a hetero-nanoarray consisting of two kinds of enzyme: horseradish peroxidase-labeled streptavidin (HRP-SA) and alkaline phosphatase-labeled anti-FITC antibody (AP-IgG) was successfully constructed through selective attachment of biotin or fluorescein in any desired wells. Successful enzyme-heteroarray formation was confirmed by enzymatic activity analyses after purification of mixtures of enzymes and DNA waffles.
2481 related Products with: Orthogonal enzyme arrays on a DNA origami scaffold bearing size-tunable wells.AccuLadder 100 bp DNA Siz AccuLadder 1 kb DNA Size ELISA Human , Angiotensin Apo B48 Elisa Blue Feulgen DNA Ploidy AccuRuller 100bp DNA Ladd AccuRuller 100bp Plus DNA AccuRuller 1Kb DNA Ladder IpPore track etched membr IpPore track etched membr IpPore track etched membr IpPore track etched membr
#24833877 2014/05/16 Save this To Up
Use of lectin microarray to differentiate gastric cancer from gastric ulcer.To investigate the feasibility of lectin microarray for differentiating gastric cancer from gastric ulcer.
2704 related Products with: Use of lectin microarray to differentiate gastric cancer from gastric ulcer.Gastric ulcer tissue arra GI cancer (gastric, colon GI cancer (esophageal, ga Cancer Samples: Gastric Gastric cardia disease sp Top 4 types of cancer (co Top 4 types of cancer (co Tissue microarray of top Top five cancer tissue ar Tissue array of gastric d Tissue microarray of blad Tissue microarray of brea
#24411673 2014/02/03 Save this To Up
Quantitative assessment of Tn antigen in breast tissue micro-arrays using CdSe aqueous quantum dots.In this study, we examined the use of CdSe aqueous quantum dots (AQDs) each conjugated to three streptavidin as a fluorescent label to image Tn antigen expression in various breast tissues via a sandwich staining procedure where the primary monoclonal anti-Tn antibody was bound to the Tn antigen on the tissue, a biotin-labeled secondary antibody was bound to the primary anti-Tn antibody, and finally the streptavidin-conjugated AQDs were bound to the biotin on the secondary antibody. We evaluated the AQD staining of Tn antigen on tissue microarrays consisting of 395 cores from 115 cases including three tumor cores and one normal-tissue core from each breast cancer case and three tumor cores from each benign case. The results indicated AQD-Tn staining was positive in more than 90% of the cells in the cancer cores but not the cells in the normal-tissue cores and the benign tumor cores. As a result, AQD-Tn staining exhibited 95% sensitivity and 90% specificity in differentiating breast cancer against normal breast tissues and benign breast conditions. These results were better than the 90% sensitivity and 80% specificity exhibited by the corresponding horse radish peroxidase (HRP) staining using the same antibodies on the same tissues and those of previous studies that used different fluorescent labels to image Tn antigen. In addition to sensitivity and specificity, the current AQD-Tn staining with a definitive threshold was quantitative.
1082 related Products with: Quantitative assessment of Tn antigen in breast tissue micro-arrays using CdSe aqueous quantum dots.Breast cancer tissue arra Breast cancer (IDC) tissu Breast cancer and adjacen Breast invasive ductal ca Breast cancer tissue arra Breast cancer tissue arra Breast cancer tissue arra Breast cancer and matched Breast cancer and matched Breast cancer and matched Breast cancer tissue arra Breast cancer, carcinoma
#23499260 2013/04/22 Save this To Up
Development and application of antibody microarray for lymphocystis disease virus detection in fish.Lymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease affecting marine and freshwater fish worldwide. Here an antibody microarray was developed and employed to detect LCDV in fish. Rabbit anti-LCDV serum was arrayed on agarose gel-modified slides as capture antibody, and Cy3-conjugated anti-LCDV monoclonal antibody (MAbs) was added as detection antibody. The signals were imaged with a laser chip scanner and analyzed by corresponding software. To improve the sensitivity, different substrate binders (poly-L-lysine, MPTS, aldehyde, APES and agarose gel modified slides, and commercially available amino-modified slides), markers (fluorescein isothiocyanate, Cy3, horseradish peroxidase, biotin or colloidal gold) conjugated to anti-LCDV Mabs, and storage time of the antibody were assessed. The results showed that the antibody microarrays based on agarose gel-modified slides gave a lower detection limit of 0.55μg/ml of LCDV when Cy3 and HRP conjugated anti-LCDV MAbs were used as detection antibody; and the lowest detectable LCDV protein concentration was 0.0686 μg/ml when streptavidin-biotin conjugated to anti-LCDV MAbs served as detection antibody. The developed antibody microarray proved to have a high specificity for LCDV detection and a shelf-life of more than 8 months at -20°C. Furthermore, the LCDV detection results of the microarray in fish gills or fins (n=50) presented a concordance rate of 100% with enzyme-linked immunosorbent assay (ELISA) and 98% with immunofluorescence assay technique (IFAT). These results revealed that the developed antibody microarray could serve as an effective tool for diagnostic and epidemiological studies of LCDV in fish.
2131 related Products with: Development and application of antibody microarray for lymphocystis disease virus detection in fish.MOUSE ANTI CANINE DISTEMP MOUSE ANTI BOVINE ROTAVIR HCV antibody test strip, H. Pylori antibody test s Integrin β1 (CD29) Antib LPAM-1(Integrin α4, CD49 α-Internexin Antibody So INPP5F antibody Source Ra Interferon alpha-8 antibo Interferon alpha-6 antibo succinate-CoA ligase, GDP interleukin 17 receptor C
#19775154 2009/10/30 Save this To Up
Single-wall carbon nanotube forest arrays for immunoelectrochemical measurement of four protein biomarkers for prostate cancer.Protein arrays that measure multiple protein cancer biomarkers in clinical samples hold great promise for reliable early cancer detection. Herein, we report a prototype 4-unit electrochemical immunoarray based on single-wall carbon nanotube forests for the simultaneous detection of multiple protein biomarkers for prostate cancer. Immunoarray procedures were designed to measure prostate specific antigen (PSA), prostate specific membrane antigen (PSMA), platelet factor-4 (PF-4), and interleukin-6 (IL-6) simultaneously in a single serum sample. All of these proteins are elevated in serum of patients with prostate cancer, but they have widely different relative levels of serum concentration. Horseradish peroxidase (HRP) was used as label on detection (secondary) antibodies in a sandwich immunoassay scheme. Biotinylated secondary antibodies (Ab(2)) that bind specifically to streptavidin-HRP conjugates provided 14-16 labels per antibody and gave the necessary higher sensitivity required for PF-4 and IL-6 detection at physiological levels. Conventional singly labeled Ab(2)-HRP conjugates were sufficient for PSA and PSMA detection. Immunoarrays were used to measure four biomarkers in clinical human serum samples of prostate cancer patients and controls with excellent correlation to referee enzyme-linked immunosorbent (ELISA) assays.
2831 related Products with: Single-wall carbon nanotube forest arrays for immunoelectrochemical measurement of four protein biomarkers for prostate cancer.Bone Morphogenetic Protei COOH Modified Single Wall Protein A (Liquid form) Protein A (Liquid form) Protein A (Liquid form) Protein A (Liquid form) NATIVE HUMAN PROLACTIN, P Native Human Lactoferrin, NATIVE HUMAN PROLACTIN, P Anti-daf-2(Abnormal dauer Anti daf 2(Abnormal dauer Anti-BMP-1 (Bone Morphoge
#19447833 2009/07/29 Save this To Up
Biotin-free systems provide stronger immunohistochemical signal in oestrogen receptor evaluation of breast cancer.Biotin-free polymeric visualisation systems (BFPS) were compared with streptavidin-biotin systems (SABS) in the evaluation of immunoreactivity for oestrogen receptor (ER) in breast carcinomas.
2413 related Products with: Biotin-free systems provide stronger immunohistochemical signal in oestrogen receptor evaluation of breast cancer.IGF-1R Signaling Phospho- T-Cell Receptor Signaling Breast cancer tissue arra Breast cancer (IDC) tissu Breast cancer and adjacen Breast cancer tissue arra Breast cancer tissue arra Breast cancer tissue arra Breast cancer and matched Breast cancer and matched Tissue microarray of brea Breast cancer and matched
#17936257 2007/11/12 Save this To Up
A quantitative analysis of N-myc downstream regulated gene 2 (NDRG 2) in human tissues and cell lysates by reverse-phase protein microarray.N-myc downstream regulated gene 2 (NDRG2) belongs to the NDRG family, which is comprised of 4 members, NDRG1-4. Recently, NDRG2 was reported as a new candidate for a tumor suppressor gene. We developed a reverse-phase protein microarray assay to access NDRG2 levels in human tissue specimens and cell lines.
2227 related Products with: A quantitative analysis of N-myc downstream regulated gene 2 (NDRG 2) in human tissues and cell lysates by reverse-phase protein microarray.Rabbit Anti-Cell death in Rabbit Anti-Cell death in Astra Blue 6GLL, Stain fo Rabbit Anti-APIP Apaf1 In Rabbit Anti-APIP Apaf1 In Rabbit Anti-IEX1 Differen Rabbit Anti-IEX1 Differen Rabbit Anti-TNIP2 ABIN2 T Rabbit Anti-TNIP2 ABIN2 T Proteins and Antibodies H Oral cavity squamous cell Alamar Blue™, REDOX ind
#15020793 2004/03/15 Save this To Up
Cytokine protein arrays.Cytokines play important roles in many aspects of cell physiology and pathology. The simultaneous determination of multiple cytokine-expression levels is receiving much attention in the research community. Multiple cytokine-expression levels can be simultaneously determined using enzyme-linked immunosorbent assay (ELISA)-based protein array technology. In this approach, target proteins are captured by the arrayed capture antibody and then detected in a sandwich ELISA format using a cocktail of biotinylated detection antibodies. The signals are visualized by either horseradish peroxidase (HRP)-conjugated streptavidin and enhanced chemiluminescence, or cy3-conjugated streptavidin and laser scanner. Several key factors and steps are described, including selection of solid supports, selection of suitable antibodies, determination of specificity and sensitivity of cytokine protein arrays, array design, sample preparation, and detailed experimental procedures for macroarray and microarray formats. An account of the successful development and application of cytokine protein arrays is presented.
Cytokine (Human) Antibody Cytokine (Human) Antibody Cytokine (Human) Antibody Cytokine (Human) Antibody Cytokine (Human) Antibody Cytokine (Human) Antibody Cytokine (Human) Antibody Cytokine (Mouse) Antibody Cytokine (Mouse) Antibody Cytokine (Mouse) Antibody Cytokine (Mouse) Antibody Cytokine (Mouse) Antibody
#11464511 2001/07/23 Save this To Up
Array-based ELISAs for high-throughput analysis of human cytokines.In this report, we describe the development of a mini-array system suitable for high-throughput quantification of proteins. This mini-array is a multiplexed, sandwich-type ELISA that measures the concentration of seven different human cytokines--TNF-alpha, IFN alpha, IFN gamma, IL-1 alpha, IL-1 beta, IL-6, and IL-10--from a single sample in each well of a 96-well plate. The mini-array is produced by spotting monoclonal antibodies (mAbs) in a 3 x 3 pattern in the bottom of the wells of 96-well polystyrene plates. Cytokines that are captured by the arrayed mAbs are detected by using biotinylated mAbs, followed by the addition of a streptavidin-horseradish peroxidase (HRP) conjugate and a chemiluminescent substrate. The light produced from the HRP-catalyzed oxidation of the substrate is measured at each spot in the array by imaging the entire plate with a commercially available CCD camera. Here, we demonstrate that these 96-well-plate format mini-arrays have performance characteristics that make them suitable for the high-throughput screening of anti-inflammatory compounds.
Cytokine (Human) Quantita Cytokine (Human) Quantita Cytokine (Human) Quantita Cytokine (Human) Quantita Cytokine (Human) Quantita IGF-1R Signaling (Human) Inflammation (Human) Quan Inflammation (Human) Quan Inflammation (Human) Quan Receptor (Human) Quantita Th1 Th2 (Human) Quantitat Th17 (Human) Quantitative
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