Search results for: anti-5-Methylcytosine (anti-5-meC) antibody mouse IgM (clone 5MC-CD) Antibodies
#30805388 // Save this To Up
Monoclonal Antibody Production Against Vimentin by Whole Cell Immunization in a Mouse Model.Pancreatic carcinoma is the fourth-leading cause of cancer death in the United States and due to its late presentation, only few patients would be candidates for the curative treatment of pancreactomy. Monoclonal antibodies have brought hope to targeted therapy.
2115 related Products with: Monoclonal Antibody Production Against Vimentin by Whole Cell Immunization in a Mouse Model.Mouse Anti-Insulin(1G11) Mouse Anti-Insulin(1G11) Mouse Anti-Insulin(1D4 ) Rabbit Anti-Cell death in Mouse Anti-Insulin(1D4 ) Anti AGO2 Mouse, Monoclon Mouse Anti-Insulin(1G11) Mouse Anti-Insulin(1G11) Mouse Anti-Insulin(1D4:) Mouse Anti-Insulin(1D4 ) Mouse Anti-Insulin(1G11) Mouse Anti-Insulin(1G11)
#30594924 // Save this To Up
Tomato endo beta-mannanase: A candidate of potential tomato allergen protein detected with human monoclonal antibody established from a patient suffered from Japanese cedar pollinosis.Peripheral blood lymphocytes from a patient allergic to Japanese cedar pollens were transformed by Epstein-Barr virus infection. Some transformed B-lymphoblastoid cells (BLCs) secreted IgM class antibodies to cedar pollen extracts and tomato fruit extracts. One stable human-mouse hybridoma clone Y-22-3-3 secreting IgM class monoclonal antibody to tomato fruit extracts was established by cell fusion of BLCs with mouse myeloma cells. Western blot analysis of tomato extracts showed Y-22-3-3 monoclonal antibody recognized a tomato protein with a molecular weight of 40 kDa. The CBB-stained 40 kDa protein from antibody-affinity chromatography was analyzed by MALDI-TOF/TOF, and identified as tomato endo-beta-mannanase, which was previously reported as one of the potential candidates for tomato allergens.
2126 related Products with: Tomato endo beta-mannanase: A candidate of potential tomato allergen protein detected with human monoclonal antibody established from a patient suffered from Japanese cedar pollinosis.Anti C Reactive Protein A amyloid beta precursor pr stress-associated endopla Mouse Monoclonal Antibody Rabbit Anti-P2RX4 ATP gat Breast cancer membrane pr Tubby-related protein 1 a Mouse Anti-Human Endorphi F-box only protein 2 anti zinc finger protein 574 a ribosomal protein S3a ant Homeobox-containing prote
#30282350 // Save this To Up
Production and characterization of monoclonal antibodies against recombinant extracellular domain of CD99.CD99/MIC2 gene product is a heavily glycosylated transmembrane protein which plays a major role in homotypic cell adhesion, apoptosis of double positive T cells and vesicular protein trafficking. It is over expressed in various cancers and has been considered as an ideal therapeutic target. The present study focused at developing monoclonal antibodies against the extracellular domain (ECD) of CD99 using hybridoma technology.
1337 related Products with: Production and characterization of monoclonal antibodies against recombinant extracellular domain of CD99.Viral antibodies: anti-H Hsp90 total Monoclonals A Monoclonal Anti dEGF Rece Monoclonal Anti-dEGF Rece Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Rat monoclonal anti mouse Mouse Anti-Human CD99 Ant Rat monoclonal anti mouse
#30280273 // Save this To Up
A Cell Assay for Detecting Anti-PEG Immune Response against PEG-Modified Therapeutics.Immunogenicity of PEGylated proteins and nanomedicines represents a potential impediment against their development and use in clinical settings. The purpose of this study is to develop a method for detecting anti-PEG immunity of PEGylated proteins and/or nanomedicines using flow cytometry.
2488 related Products with: A Cell Assay for Detecting Anti-PEG Immune Response against PEG-Modified Therapeutics.MarkerGeneTM Fluorescent MarkerGene™ LysoLive™ Mouse Anti-Human CD40 (CD Quick Cell Proliferation Rabbit Anti-SF9 Insect Ce Mouse Anti-Human CD19 (pa MOUSE ANTI HUMAN CD15, Pr MarkerGeneTM Gaussia Luci Multiple lung carcinoma ( Rabbit Anti-Cell death in RubyGlowTM Luminescent Ce Rabbit Anti-Cell death in
#30031120 // Save this To Up
Production of a monoclonal antibody against a mannose-binding protein of Acanthamoeba culbertsoni and its localization.Amoebae from the genus Acanthamoeba are facultative pathogens of humans and other animals. In humans they most frequently infect the eye causing a sight threatening infection known as Acanthamoeba keratitis (AK), and also cause an often fatal encephalitis (GAE). A mannose-binding protein (MBP) has been identified as being important for Acanthamoeba infection especially in AK. This lectin has previously been characterized from Acanthamoeba castellanii as consisting of multiple 130 kDa subunits. MBP expression correlates with pathogenic potential and is expressed in a number of Acanthamoeba species. Here we report the purification of a similar lectin from Acanthamoeba culbertsoni and the production of a monoclonal antibody to it. The A. culbertsoni MBP was isolated by affinity chromatography using α-D-mannose agarose and has an apparent molecular weight of 83 kDa. The monoclonal antibody is an IgM that is useful in both western blots and immunofluorescence. We expect that this antibody will be useful in the study of the pathology of A. culbertsoni and in its identification in clinical samples.
1877 related Products with: Production of a monoclonal antibody against a mannose-binding protein of Acanthamoeba culbertsoni and its localization.Anti-actin binding protei Anti AGE 3 Monoclonal Ant Monoclonal Anti-C-Reactiv Mouse Anti-F1 protein (YE ReadiLink™ mFluor™ Vi Anti-actin binding protei Rabbit Anti-F1 protein (Y Rat monoclonal anti mouse DNA Binding Protein 7 (DB Anti Galectin(Gal 3) Huma RNA binding motif protein anti-Vitamin D binding pr
#29632541 // Save this To Up
5' Rapid Amplification of cDNA Ends and Illumina MiSeq Reveals B Cell Receptor Features in Healthy Adults, Adults With Chronic HIV-1 Infection, Cord Blood, and Humanized Mice.Using 5' rapid amplification of cDNA ends, Illumina MiSeq, and basic flow cytometry, we systematically analyzed the expressed B cell receptor (BCR) repertoire in 14 healthy adult PBMCs, 5 HIV-1+ adult PBMCs, 5 cord blood samples, and 3 HIS-CD4/B mice, examining the full-length variable region of μ, γ, α, κ, and λ chains for V-gene usage, somatic hypermutation (SHM), and CDR3 length. Adding to the known repertoire of healthy adults, Illumina MiSeq consistently detected small fractions of reads with high mutation frequencies including hypermutated μ reads, and reads with long CDR3s. Additionally, the less studied IgA repertoire displayed similar characteristics to that of IgG. Compared to healthy adults, the five HIV-1 chronically infected adults displayed elevated mutation frequencies for all μ, γ, α, κ, and λ chains examined and slightly longer CDR3 lengths for γ, α, and λ. To evaluate the reconstituted human BCR sequences in a humanized mouse model, we analyzed cord blood and HIS-CD4/B mice, which all lacked the typical SHM seen in the adult reference. Furthermore, MiSeq revealed identical unmutated IgM sequences derived from separate cell aliquots, thus for the first time demonstrating rare clonal members of unmutated IgM B cells by sequencing.
1797 related Products with: 5' Rapid Amplification of cDNA Ends and Illumina MiSeq Reveals B Cell Receptor Features in Healthy Adults, Adults With Chronic HIV-1 Infection, Cord Blood, and Humanized Mice.Androgen Receptor Androgen Receptor (Phosph Rabbit Anti-Insulin Recep Bovine Red Blood Cells, P Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Sheep Red Blood Cells, Pa Rabbit Anti-Insulin Recep Rabbit Anti-Insulin Recep Bovine Red Blood Cells, P Human Cord Blood CD34+ Ce Guinea Pig Red Blood Cell
#29420136 // Save this To Up
Preparation, Purification, and Identification of a Monoclonal Antibody Against the C-Terminal Domain of Semaphorin3F.Class three semaphorins were originally identified as mediators of axon guidance, which repelled axons and collapsed growth cones. As a member of class three semaphorins, semaphorin3F (Sema3F) has been found to have similar effects on tumor cells and endothelial cells and also is implicated in the signaling of tumor metastasis by forming a complex with neuropilins and plexins. In this study, our laboratory produced a monoclonal antibody against the C-terminal domain of Sema3F (Sema3Fc mAb) using the hybridoma method, expecting to explore the potential role of the antibody and its application in the detection of Sema3F. The capture enzyme-linked immunosorbent assay (ELISA) method indicated that mAb belonged to the IgM subclass and purified Sema3Fc mAb had a titer of 5.12 × 10 against Sema3Fc by indirect ELISA. In addition, results showed that the Sema3Fc mAb could be applied in such experiments as Western blotting, flow cytometry, immunofluorescence, and immunocytochemical staining. It indicates the Sema3Fc mAb is available in the detection of Sema3F with specificity and will help further study the role and mechanism of Sema3F among tumor cells.
1282 related Products with: Preparation, Purification, and Identification of a Monoclonal Antibody Against the C-Terminal Domain of Semaphorin3F.Monoclonal Anti-dEGF Rece FDA Standard Frozen Tissu Monoclonal Anti-Cellulose Apoptosis Repressor with Monoclonal Anti-dEGF Rece Apoptosis Repressor with FDA Standard Frozen Tissu Anti-Calpain-5 (H-TRA-3) to PC4 (paired basic ami Anti-Axin1 (C-terminal) p Anti CML Monoclonal Antib Anti-BPDE Monoclonal Anti
#29266242 // Save this To Up
Age-associated B cells expanded in autoimmune mice are memory cells sharing H-CDR3-selected repertoires.Age-associated B cells (ABCs) represent a distinct cell population expressing low levels of CD21 (CD21 ). The Ig repertoire expressed by ABCs in aged mice is diverse and exhibits signs of somatic hypermutation (SHM). A CD21 B-cell population is expanded in autoimmune diseases, e.g. systemic lupus erythematosus, as well as in lupus-prone NZB/W mice and in mice lacking a pre-B cell receptor (SLC ). However, the nature of the CD21 B cells (hereafter ABCs) in autoimmunity is not well understood. Here we show that in young SLC mice, the vast majority of the ABCs express memory B-cell (MBC) markers in contrast to wild-type controls. A similar population is present in lupus-prone MRL mice before and at disease onset. In SLC mice, a majority of the ABCs are IgM , their V genes have undergone SHM, show clonal diversification and clonal restriction at the H-CDR3 level. ABC hybridomas, established from SLC mice, secrete typical lupus autoantibodies, e.g. anti-Smith antigen, and some of those that bind to DNA comprise a H-CDR3 that is identical to previously described IgM anti-DNA antibodies from lupus-prone mice. Together, these results reveal that ABCs in autoimmune mice are comprised of autoreactive MBCs expressing highly restricted H-CDR3 repertoires.
2387 related Products with: Age-associated B cells expanded in autoimmune mice are memory cells sharing H-CDR3-selected repertoires.superSf9-1 insect cells Cold Fusion Cloning Kit w Human Small Intestine Mic Leptin ELISA Kit, Rat Lep CometAssay Electrophoresi anti HSV (II) gB IgG1 (mo GLP 1 ELISA Kit, Rat Gluc superSf9-3 insect cells Macrophage Colony Stimula Nile Red, A lipophilic dy Human Internal Mammary Ar Rat Anti-Mouse Dendritic
#29174537 // Save this To Up
Flow cytometry-based method for rapid and high-throughput screening of hybridoma cells secreting monoclonal antibody.Monoclonal antibodies (mAbs) are a valuable biomaterial for basic life sciences and industrial purposes. The production of the mAb is time and effort intensive. In this report, we established a time- and labor-saving method for the mAb production. Because membrane-type immunoglobulin on a hybridoma cell surface and its secreted form, called as antibody, share the same binding property to the antigen, the fluorescence-labeled antigen bound to membrane-type immunoglobulin can be used as a screening marker. In the method, a hybridoma labeled by a fluorescent antigen was selected and sorted singly into 96-well plate using flow cytometer. Model experiments indicated that the method is highly efficient to obtain good mAbs suitable for Western blotting and immunofluorescence. Notably, most mAbs established by this method belonged to the IgG isotype, which is preferred over the IgM counterpart. Using a high-throughput flow cytometer, the method avoids tedious repeated screening and cloning processes. Because the method uses conventional myeloma for cell fusion and all reagents required in this method are commercially available, all research laboratories can apply the method to obtain mAbs efficiently.
2198 related Products with: Flow cytometry-based method for rapid and high-throughput screening of hybridoma cells secreting monoclonal antibody.Anti C Reactive Protein A EnzyChrom™ Kinase Assay MOUSE ANTI APAAP COMPLEX, MOUSE ANTI BORRELIA BURGD Rat L-90 Array, Membrane Mouse L-308 Array, Membra Anti-bodywall muscle cell Anti-apoptotic marker in MOUSE ANTI HUMAN CD15, Pr Rat L-90 Array, Glass Sli Mouse L-308 Array, Glass MOUSE ANTI BOVINE ROTAVIR
#28719245 // Save this To Up
Production of a Mouse Monoclonal Antibody Against Mortalin by Whole Cell Immunization.Pancreatic carcinoma is the fourth leading cause of cancer death and is characterized by early invasion and metastasis. Advances in molecular biology directed new strategies in targeted therapy using monoclonal antibodies. To identify new biomarkers, we generated a panel of monoclonal antibodies against the newly established cell line, Faraz-ICR, from a patient with acinar cell carcinoma. After immunization of BALB/c female mice with Faraz-ICR cell line and fusion of splenocytes with SP2/0 myeloma cell line, high reactive hybridoma producing antibodies to Faraz-ICR were detected using enzyme-linked immunosorbent assay, immunofluorescence staining and flow cytometry. Western blot and two-dimensional immunoblot were used for further characterization of the targets antibodies. Among high reactive clones, the reactivity of 1C11 clone was assessed with other epithelial tumors. The isotype of the antibody was revealed to be IgM, and the antibody reacted to a protein with a molecular weight of about 70 kDa in Western blot analysis. To further characterization of the target antigen, immunoproteome of the Faraz-ICR cell line was performed. By liquid chromatography-mass spectrometry (LC-MS) analysis, we identified that the target of 1C11 clone was HSP70. In conclusion, pancreatic cancer is a fatal malignancy with no reliable biomarker for early screening and diagnosis. In this study, by establishing a pancreatic cell line, a panel of monoclonal antibodies was generated aiming to explore specific or associated cancer targets. We then introduced 1C11 monoclonal antibody that can specifically recognize mortalin as a main tumor marker and may serve as a new tool for diagnostic kit and therapeutic strategies targeting this molecule.
2915 related Products with: Production of a Mouse Monoclonal Antibody Against Mortalin by Whole Cell Immunization.Monoclonal Anti-Cellulose Mouse Anti-GAPDH(3E12)-Lo Antibodies, Mouse: Monoc Monoclonal antibody: Hum Mouse Anti-Insulin(1G11) Mouse Anti-Transferrin(1F Mouse Anti-Insulin(1G11) Mouse Anti-Insulin(1D4:) Mouse Anti-Insulin(1D4 ) Mouse Anti-BrdU(A7) Monoc MOUSE ANTI HUMAN CD89-MON Mouse Anti-BrdU(A7) Monoc
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 Fax 0032 16 50 90 45
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50 Fax 01 43 25 01 60
52062 Aachen Deutschland
Tel 0241 40 08 90 86 Fax 0241 55 91 05 36
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
Schweiz Züri +41435006251
Česká republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Ελλάς Αθήνα +302111768494
Magyarország Budapest +3619980547
GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
GENTAUR Nederland BV
5521 DG Eersel Nederland
Tel 0208-080893 Fax 0497-517897
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93 Fax 02 36 00 65 94
53 Iskar Str. 1191 Kokalyane, Sofia