Only in Titles

           Search results for: anti-BrdU antibody   

paperclip

#7524812   1994/11/29 Save this To Up

PCNA/cyclin expression and BrdU uptake define proliferating myosatellite cells during hyperplastic muscle growth of fish (Cyprinus carpio L.).

The possibility of detecting in situ proliferating myosatellite cells during postlarval muscle growth in the carp (Cyprinus carpio, L.) by means of BrdU and PCNA (Cyclin) immunohistochemistry has been evaluated on paraffin embedded sections. Nine subadult stages were defined according to the body length and weight. The fish were injected intraperitoneally with BrdU and fixed one hour later. Adjacent cross sections mounted on glass slides were incubated with monoclonal antiBrdU (1:100) and antiPCNA (1:200) antibody. The proliferative rate, defined as the percentage of labelled cells for each stage, was correlated to the corresponding percentage of small fibers (area less than 200 microns 2) determined by morphometric analysis. Desmin expression, immunocytochemically detected, was aimed at discriminating between the postmitotic and stem myosatellite cells. A low myosatellite cell proliferative activity (2-7%) throughout the carp growth period considered was demonstrated. Quantitative analysis provided evidence that during growth stages in which small fibers are numerous, the myosatellite cell proliferative activity is low and it increases when the small fibers number decreases. It is suggested that an age dependence of myosatellite cell proliferative rate controls the recruitment of new fibers in the carp.

1139 related Products with: PCNA/cyclin expression and BrdU uptake define proliferating myosatellite cells during hyperplastic muscle growth of fish (Cyprinus carpio L.).

Epidermal Growth Factor ( Epidermal Growth Factor ( pDC57 Mammalian Luciferas pDC99 Mammalian Luciferas Mouse Anti-Human Prolifer Anti-bodywall muscle cell Anti bodywall muscle cell Anti-bodywall muscle cell Anti bodywall muscle cell Anti-bodywall muscle cell Anti bodywall muscle cell Anti-bodywall muscle cell

Related Pathways

paperclip

#7688604   1993/09/13 Save this To Up

Use of 5'-bromodeoxyuridine immunohistochemistry to examine proliferative activity of fish tissues.

The suitability of bromodeoxyuridine (BrdU) immunohistochemistry for the study of myosatellite cell proliferation in three subadult carp (Cyprinus carpio) stages (11, 15, 17 cm) was examined. They were injected intraperitoneally with BrdU and fixed one hour late. After fixation and dehydration, white myotomal muscle and small intestine samples were embedded in Histowax. Cross sections mounted on glass slides, were incubated with monoclonal antiBrdU antibody (1:100) after HCl denaturation. After washing twice in PBS, slides were incubated in goat antimouse IgG FITC secondary antibody (1:20). Single cell suspensions were obtained from gut samples. Cellular DNA partially denatured with 2 N HCl, were immunolabelled with monoclonal antibodies against BrdU. Bivariate distribution of BrdU/total cellular DNA content was measured by flow cytometry. Good visualization of BrdU labelled myosatellite cells (4-6%) and small intestine (8-9%) was obtained. Flow cytometric bivariate BrdU/DNA analysis gave evidence of the same proliferative rate in the gut samples. The applicability of these methods to fish tissues further extend the broad range of biological and biomedical investigation in which BrdU immunohistochemistry has been used.

2199 related Products with: Use of 5'-bromodeoxyuridine immunohistochemistry to examine proliferative activity of fish tissues.

Cell Meter™ Fluorimetri Cell Meter™ Fluorimetri Alkaline Phospatase (ALP) AccuzolTM Total RNA Extra MarkerGeneTM Live Cell Gl MarkerGene™ â Galactos Ofloxacin CAS Number [824 Rapid Microplate Assay K Topoisomerase II; Clone Topoisomerase II; Clone Topoisomerase II; Clone Toludine Blue Solution

Related Pathways