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#38648108 2024/04/22 To Up
Methods for screening and evaluation of antimicrobial activity: A review of protocols, advantages, and limitations.
Infectious diseases pose a formidable global challenge, compounded by the emergence of antimicrobial resistance. Consequently, researchers are actively exploring novel antimicrobial compounds as potential solutions. This endeavor underscores the pivotal role of methods employed for screening and evaluating antimicrobial activity-a critical step in discovery and characterization of antimicrobial agents. While traditional techniques such as well-diffusion, disk-diffusion, and broth-dilution are commonly utilized in antimicrobial assays, they may encounter limitations concerning reproducibility and speed. Additionally, a diverse array of antimicrobial assays including cross-streaking, poisoned-food, co-culture, time-kill kinetics, resazurin assay, bioautography, etc., are routinely employed in antimicrobial evaluations. Advanced techniques such as flow-cytometry, impedance analysis, and bioluminescent technique may offer rapid and sensitive results, providing deeper insights into the impact of antimicrobials on cellular integrity. However, their higher cost and limited accessibility in certain laboratory settings may present challenges. This article provides a comprehensive overview of assays designed to characterize antimicrobial activity, elucidating their underlying principles, protocols, advantages, and limitations. The primary objective is to enhance understanding of the methodologies designed for evaluating antimicrobial agents in our relentless battle against infectious diseases. By selecting the appropriate antimicrobial testing method, researchers can discern suitable conditions and streamline the identification of effective antimicrobial agents.Tanim Jabid Hossain
2353 related Products with: Methods for screening and evaluation of antimicrobial activity: A review of protocols, advantages, and limitations.
100ug50 mg25 mg100ug96T50 ug 10 mg1000 tests100ul1000 TESTS/0.65ml500 mg25 mgRelated Pathways
#38648039 // To Up
Levels of the HtrA1 Protein in Serum and Vitreous Humor Are Independent of Genetic Risk for Age-Related Macular Degeneration at the 10q26 Locus.
The purpose of this study was to determine if levels of the HtrA1 protein in serum or vitreous humor are influenced by genetic risk for age-related macular degeneration (AMD) at the 10q26 locus, age, sex, AMD status, and/or AMD disease severity, and, therefore, to determine the contribution of systemic and ocular HtrA1 to the AMD disease process.Brandi L Williams, Moussa A Zouache, Nathan A Seager, Chris M Pappas, Jin Liu, Robert A Anstadt, William C Hubbard, Julie Thomas, Jill L Hageman, Jennifer Mohler, Burt T Richards, Gregory S Hageman
2212 related Products with: Levels of the HtrA1 Protein in Serum and Vitreous Humor Are Independent of Genetic Risk for Age-Related Macular Degeneration at the 10q26 Locus.
96T100 U2 Pieces/Box2 Pieces/Box1 Set1mg1Related Pathways
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#38647967 2024/02/01 To Up
Production of biliverdin by biotransformation of exogenous heme using recombinant Pichia pastoris cells.
BJianfeng Mei, Yanchao Han, Shihang Zhuang, Zhikai Yang, Yu Yi, Guoqing Ying
2386 related Products with: Production of biliverdin by biotransformation of exogenous heme using recombinant Pichia pastoris cells.
1 mg100 µg1001mg1mg21 mg1mg201mg1001 mgRelated Pathways
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#38647905 2024/03/05 To Up
Optimization of fungicidal and acaricidal metabolite production by endophytic fungus Aspergillus sp. SPH2.
The endophytic fungus Aspergillus sp. SPH2 was isolated from the stems of the endemic plant Bethencourtia palmensis and its extracts were found to have strong fungicidal effects against Botrytis cinerea and ixodicidal effects against Hyalomma lusitanicum at different fermentation times. In this study, the fungus was grown using three different culture media and two methodologies, Microparticulate Enhancement Cultivation (MPEC) and Semi-Solid-State Fermentation (Semi-SSF), to increase the production of secondary metabolites during submerged fermentation. The addition of an inert support to the culture medium (Semi-SSF) resulted in a significant increase in the extract production. However, when talcum powder was added to different culture media, unexpected results were observed, with a decrease in the production of the biocompounds of interest. Metabolomic analyses showed that the production of aspergillic, neoaspergillic, and neohydroxyaspergillic acids peaked in the first few days of fermentation, with notable differences observed among the methodologies and culture media. Mellein production was particularly affected by the addition of an inert support to the culture medium. These results highlight the importance of surface properties and morphology of spores and mycelia during fermentation by this fungal species.Nicolas Reyes Castillo, Carmen E Díaz, M Fe Andres, Juan Imperial, Félix Valcárcel, Ana Azucena González Coloma
1610 related Products with: Optimization of fungicidal and acaricidal metabolite production by endophytic fungus Aspergillus sp. SPH2.
100ul100 mg100ul 1 kit(s) 200ul200 µg10 mg100 mg100ug 25 MG100μg1 mgRelated Pathways
#38647902 2023/11/09 To Up
Influence of light absorption rate on the astaxanthin production by the microalga Haematococcus pluvialis during nitrogen starvation.
Large amounts of astaxanthin (about 4% DW) can be produced under nitrogen starvation of Haematococcus pluvialis in photobioreactors (PBRs) exposed to high light conditions to induce a light stress. However, in PBR, the large biomass concentration usually achieved leads to strong light attenuation conditions, which makes complex the analysis of this "light stress". This study aims to elucidate the role of light transfer in astaxanthin cell content and productivity from the microalga Haematococcus pluvialis during nitrogen starvation. Haematococcus pluvialis was cultivated in a flat-panel PBR in a batch mode with sudden nitrogen starvation conditions and an incident photon flux density (PFD) of 250 µmol m s. Different initial biomass concentrations ( ) were evaluated, 0.21, 0.52, 1.39 and 2.21 kg m. As a result, spectral mass absorption cross-sections of Haematococcus pluvialis were measured at different times during nitrogen starvation, and were used to relate the mean rate of photon absorption (MRPA) to the astaxanthin productivity. A minimum initial MRPA of 7000 ± 500 µmol kg s was found necessary to trigger large accumulation of astaxanthin in Haematococcus pluvialis cells (up to 3.21% DW) during nitrogen starvation conditions. The results also demonstrated the link between the MRPA and the daily astaxanthin productivity of Haematococcus pluvialis cultures, introducing then the MRPA as a physical quantity of interest for a rational optimization of the light culture conditions in PBRs.Khadija Samhat, Antoinette Kazbar, Hosni Takache, Ali Ismail, Jeremy Pruvost
1532 related Products with: Influence of light absorption rate on the astaxanthin production by the microalga Haematococcus pluvialis during nitrogen starvation.
500IUmin 2 cartons100.00 ul1200 units11 ml1500 UnitsRelated Pathways
#38647878 2023/03/02 To Up
Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction.
Lipids produced by oleaginous yeasts are considered as sustainable sources for the production of biofuels and oleochemicals. The red yeast Rhodosporidium toruloides can accumulate lipids to over 70% of its dry cell mass. To facilitate lipid extraction, a recombinant β-1,3-glucomannanase, MAN5C, has been applied to partially breakdown R. toruloides cell wall. In this study, R. toruloides NP11 was engineered for secretory expression of MAN5C to simplify the lipid extraction process. Specifically, a cassette contained a codon-optimized gene MAN5C was integrated into the genome of R. toruloides by Agrobacterium-mediated transformation. The engineered strain NP11-MAN5C was found with proper expression and secretion of active MAN5C, yet no notable compromise in terms of cell growth and lipid production. When NP11-MAN5C cell cultures were extracted with ethyl acetate without any pretreatment, 20% of total lipids were recovered, 4.3-fold higher than that of the parental strain NP11. When the cells were heat-treated followed by extraction with ethyl acetate in the presence of the culture broth supernatants, up to 93% of total lipids were recovered, confirming beneficial effects of MAN5C produced in situ. This study provides a new strategy to engineer oleaginous yeasts for more viable lipid extraction and down-stream processes.Shiyu Liang, Yue Zhang, Liting Lyu, Shuang Wang, Zongbao K Zhao
1022 related Products with: Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction.
1 G 1 G100 mg300 units 5 G10 mgRelated Pathways
#38647868 2024/04/22 To Up
Changing Patterns of Organisms Causing Neonatal Sepsis and Their Antimicrobial Sensitivity Profile in a Tertiary Center - A Prospective Study.
To identify the profile of organisms causing neonatal sepsis and their antibiotic susceptibility pattern in recent years.Gayathiri Govindaraju, Balakrishnan Rajaiah, Srinivas Ramakrishnan, Deepak Thangaraju, Preethi Chandrasekar, Thangaraj Abiramalatha
2557 related Products with: Changing Patterns of Organisms Causing Neonatal Sepsis and Their Antimicrobial Sensitivity Profile in a Tertiary Center - A Prospective Study.
100 ug96T1mg100 μg100ug Lyophilized1 mg100 μg100ugRelated Pathways
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