Search results for: elisa kits
#25358091 2014/10/30 Save this To Up
The Effects of Breastfeeding on Serum Asymmetric Dimethylarginine Levels and Body Composition in Children.Abstract Introduction: The purpose of this work was to investigate the association of serum asymmetric dimethylarginine (ADMA) and high-sensitivity C-reactive protein (hs-CRP) levels with duration of breastfeeding and body composition in children. Patients and Methods: The study group consisted of 88 patients with a median age of 12 months (42 boys, 46 girls), classified as never breastfed or fully breastfed. ADMA and hs-CRP were measured by immunoenzymatic enzyme-linked immunosorbent assay commercial kits. Body composition analysis was performed by bioelectrical impedance. Results: We found significantly higher serum ADMA levels but not serum hs-CRP levels in never breastfed compared with the fully breastfed group (p<0.05). Serum ADMA was inversely associated with high-density lipoprotein-cholesterol levels and breastfeeding duration (p<0.05). Positive correlation was found between ADMA and body fat mass (p<0.05). Conclusions: In never breastfed children, increased ADMA is observed; however, further studies are needed to assess whether breastfeeding duration affects body fat and body composition at older ages.
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#25345931 2014/10/27 Save this To Up
[Exendin-4 promotes paracrine action of adipose-derived stem cells through PI3K/Akt signaling pathways].To investigate the mechanism by which exendin-4 promotes paracrine secretion of cytokines by adipose-derived stem cells (ADSCs).
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#25345339 2014/10/27 Save this To Up
Correlation of TLR-4, IL-18, Transaminases and Uric Acid in the Patients With Chronic Periodontitis and Healthy Adults.Background: Because of the potential association between periodontal disease and inflammation, the purpose of the present study was to examine the level of toll like receptor-4 (TLR-4), Interleukin-18 (IL-18) and uric acid as a marker of the inflammatory host response along with routine biochemical parameters such as fasting glucose, insulin, total cholesterol, High density lipoprotein (HDL), Low density lipoprotein (LDL), triglycerides, Fasting glucose, Alanine transaminase (AST), Aspartate transaminase (ALT) in the plasma and saliva of healthy individuals and periodontitis patients. We also wanted to check whether the patients with chronic periodontitis exhibit different modulations in salivary and/or plasma concentrations of these parameters compared with clinically healthy individuals. Materials and methods: Both salivary and plasma samples were collected from 40 chronic periodontitis patients and 20 healthy control individuals. TLR-4 and IL-18 measurements were done using commercially available ELISA kits. Total cholesterol, HDL, LDL, triglycerides, Fasting glucose, AST and ALT levels were analyzed on Roche p-800 modular system using the specific kits provided by the manufacturer. Non-parametric tests was used in case of certain parameters for the statistical analyses, because our data did not follow the Gaussian distribution. Results: Significant difference was observed in the plasma and salivary TLR-4 and IL-18 levels along with clinical measurements such as plaque index (PI) and pocket depth (PD) in chronic periodontitis patients (P<0.001). The plasma level of TLR-4 was found to be enhanced from 0.99 to 3.28 in chronic periodontitis patients. Salivary TLR-4 levels also showed slightly higher increase in diseased state (12.44 to 29.97). A significant increase of approx 46% was recorded in the plasma IL-18 level. However, salivary IL-18 level rose upto more than 5 fold in the chronic periodontitis patients compared with the control individuals. The level of plasma uric acid was found to be highly significant compared to control individuals. HDL and triglyceride also showed significant difference (P<0.017 and P<0.025 respectively). Moreover, plasma glucose, total cholesterol, LDL and insulin levels did not show any significant difference. There was only slight increase in the plasma AST and ALT levels between diseased and healthy state (22.55 vs 25.50 and 12.35 vs 15.95 respectively). However, salivary AST and ALT level showed approx 6 fold rise in the chronic periodontitis patients compared with the control individuals. The cross correlation analysis in periodontitis disease group showed significant association of plasma AST, salivary AST and salivary ALT with uric acid level. Conclusion: Based on our study, we believe that TLR-4, IL-18 and uric acid could have a role in the inflammatory pathology of periodontitis. These parameters are suggested to be useful in the prognosis and diagnosis of chronic periodontitis. However, mechanistic association of these parameters with inflammatory pathology of periodontitis patients needs to be further elucidated on a higher number of samples.
1621 related Products with: Correlation of TLR-4, IL-18, Transaminases and Uric Acid in the Patients With Chronic Periodontitis and Healthy Adults.Androst-4-ene-3,17-dion-1 Interleukins Recombinant Interleukins Recombinant CELLKINES Natural Human I Human Interleukin-4 IL-4 Human Interleukin-6 IL-6 Human Interleukin-7 IL-7 Human Interleukin-2 IL-2 Human Interleukin-16 IL-1 Human Interleukin-33 IL-3 Human Interleukin-17E (IL Human Interleukin-32 alph
#25339431 2014/10/23 Save this To Up
Effect of pre-partum feed supplementation on post-partum ovarian activity, milk production and calf growth of small holder dairy Cattle in Cameroon.Seventy-two cows were selected for an on-farm study on the effect of feed supplementation before calving on milk production, ovarian activity and calf growth of Holstein, indigenous Red Fulani cows and their crosses. Pre-partum feed supplementation was done using cotton seed cake (80 %), maize (18 %), bone meal (1 %) and kitchen salt (1 % NaCl). Supplementation levels consisted of a low supplementation fed at 1 kg per animal per day and high supplementation fed at 2 kg per animal per day. In addition, Red Fulani cows received the supplements in two different ways namely a pre-partum supplementation consisting of 1 kg per cow per day and pre- and post-partum supplementation consisting of 1 kg per cow per day before calving and 1 kg per cow per day post-partum up to 30 days after calving. Blood samples were analysed using ELISA Progesterone kits to determine the length of post-partum anoestrus. Results show that pre-partum levels of feeding did not have any effect (P > 0.05) on body condition score (BCS) at 12 weeks after calving, calf birth weight, average daily weight gain of calves, milk production and post-partum anoestrus. High BCS at calving was shown to influence BCS at 12 weeks of lactation. Holstein cows had bigger calves (P < 0.01) at birth (45 kg) compared to traditional cows (36 kg) and crosses (34 kg). There was little benefit of pre-partum supplementation on the parameters investigated in this study. Consequently, low income farmers are advised to concentrate their efforts of supplementation early in lactation.
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#25339322 2014/10/23 Save this To Up
[Immuno-effect of plasmacytoid dendritic cells on bacteria infection induced spontaneous remission of leukemia].To explore the immuno-effect of plasmacytoid dendritic cells (pDC) on bacteria infection induced spontaneous remission (SR) of leukemia.
2351 related Products with: [Immuno-effect of plasmacytoid dendritic cells on bacteria infection induced spontaneous remission of leukemia].Mouse Anti-Human Dendriti Rat Anti-Mouse Dendritic Mouse Anti-Human Follicul Mouse Anti-Human Follicul Mouse Anti-Human Follicul Mouse Anti-Human Follicul MarkerGeneTM in vivo lacZ MarkerGeneTM Chemilumines Octyl â D 1 thioglucopyr Ofloxacin CAS Number [824 Bcl-2 Oncoprotein; Clone Bcl-2 Oncoprotein; Clone
#25338574 2014/10/23 Save this To Up
[Effect of plasmacytoid dendritic cells activited by bacteria on spontaneous remission of leukemia].Spontaneous remission (SR) of leukemia is a rare event in clinic, which possibly correlated with severe infection and sepsis, but its exact mechanism has not been confirmed. Plasmacytoid dendritic cells (pDC) and myeloid dendritic cells (mDC) play a key role in innate and adaptive immunity respectively. A patient with severe infection of staphylococcus aureus acquired completely spontaneous remission (SR), moreover a increased number of pDC were observed, suggesting that bacteria-activated pDC may play an important role in SR. This study was purposed to explore if the bacteria can stimulate pDC successfully and get a functional pDC. Both pDC and mDC were isolated from freshly collected, leukocyte-rich buffy coats from healthy blood donor and leukemic patient with SR by using MACS and FACS. The pDC were cultured in RPMI 1640 medium and were stimulated with different kinds of bacteria and the expression of CD40, CD86 and HLA-DR on the cell surface was analyzed by flow cytometry. The cytokine (IFN-α, IL-12, IFN-γ, IL-2, IL-4, IL-10) production was measured by using ELISA kits. The results showed that the stimulation with staphylococcus aureus and pseudomonas aeruginosa resulted in the maturation of pDC, which secrete a large number of IFN-α and promote the differentiation of naive CD4(+) T cells to Th1 cells. The activated pDC expressed high level of CD40 and CD86 and showed higher T cell stimulatory capacities. It is concluded that staphylococcus aureus and pseudomonas aeruginosa can activate pDC, the activated pDC secrete high quantity of IFN-α. This result suggests that bacteria stimulated pDC may play a key role in SR of leukemia following severe infections.
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#25338345 2014/10/23 Save this To Up
Evaluation of bacterial translocation in patients with chronic HCV infection.The factors involved in the progression of liver disease towards decompensated cirrhosis are not completely elucidated. It seems that bacterial translocation (BT) from the gut to the systemic blood flow has an important role in the disease progression, but literature data are controversial. Our objectives were to evaluate the presence of BT in patients with chronic HCV infection and to assess the correlation between BT and liver fibrosis stages and inflammatory state.
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#25337367 2014/10/22 Save this To Up
The effect of food deprivation on nociception in formalin test and plasma levels of noradrenaline and corticosterone in rats.The concentration of noradrenalin and corticosterone as the two nociception modulators change after fasting or stress situation. The aim of present study was to investigate the effect of food deprivation on formalin-induced nociceptive behaviours and plasma levels of noradrenalin and corticosterone in rats.
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#25330157 2014/10/21 Save this To Up
Evaluation of Commercially Available Diagnostic Tests for the Detection of Dengue Virus NS1 Antigen and Anti-Dengue Virus IgM Antibody.Commercially available diagnostic test kits for detection of dengue virus (DENV) non-structural protein 1 (NS1) and anti-DENV IgM were evaluated for their sensitivity and specificity and other performance characteristics by a diagnostic laboratory network developed by World Health Organization (WHO), the UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR) and the Pediatric Dengue Vaccine Initiative (PDVI). Each network laboratory contributed characterized serum specimens for the panels used in the evaluation. Microplate enzyme-linked immunosorbent assay (ELISA) and rapid diagnostic test (RDT formats) were represented by the kits. Each ELISA was evaluated by 2 laboratories and RDTs were evaluated by at least 3 laboratories. The reference tests for IgM anti-DENV were laboratory developed assays produced by the Armed Forces Research Institute for Medical Science (AFRIMS) and the Centers for Disease Control and Prevention (CDC), and the NS1 reference test was reverse transcriptase polymerase chain reaction (RT-PCR). Results were analyzed to determine sensitivity, specificity, inter-laboratory and inter-reader agreement, lot-to-lot variation and ease-of-use. NS1 ELISA sensitivity was 60-75% and specificity 71-80%; NS1 RDT sensitivity was 38-71% and specificity 76-80%; the IgM anti-DENV RDTs sensitivity was 30-96%, with a specificity of 86-92%, and IgM anti-DENV ELISA sensitivity was 96-98% and specificity 78-91%. NS1 tests were generally more sensitive in specimens from the acute phase of dengue and in primary DENV infection, whereas IgM anti-DENV tests were less sensitive in secondary DENV infections. The reproducibility of the NS1 RDTs ranged from 92-99% and the IgM anti-DENV RDTs from 88-94%.
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#25329073 2014/10/21 Save this To Up
Treg/IL-17 Ratio and Treg Differentiation in Patients with COPD.Chronic obstructive pulmonary disease (COPD) is characterized by chronic pulmonary and systematic inflammation. An abnormal adaptive immune response leads to an imbalance between pro- and anti-inflammatory processes. T-helper (Th), T-cytotoxic (Tc) and T-regulatory (Treg) cells may play important roles in immune and inflammatory responses. This study was conducted to clarify the changes and imbalance of cytokines and T lymphocyte subsets in patients with COPD, especially during acute exacerbations (AECOPD).
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