Only in Titles

           Search results for: elisa kits   

paperclip

#28093042   2017/01/17 Save this To Up

Chloramphenicol stereoisomers need to be distinguished: consequences observed from a proficiency test.

Although the use of chloramphenicol (CAP) as a veterinary drug is banned in the European Union and many other countries, monitoring for CAP residues in food is routine. Positive detections are few, but taken extremely seriously. European Union laboratories analysing for CAP should validate methods according to European Commission Decision 2002/657/EC, must be accredited to ISO 17025, and will generally participate in proficiency testing (PT) schemes, such as those offered by the Food Analysis Performance Assessment Scheme (FAPAS®). The FAPAS PTs aim to cover a wide range of relevant matrices including honey, prawns, fish, milk and kidney. Test materials are prepared either by animal dosing studies or by spiking raw matrix. The most common method reported by FAPAS participants used to screen for CAP residues is LC-MS/MS, but ELISA kits are increasingly being used. A recent PT round highlighted that the result obtained might be correlated with the type of analytical method being employed. Follow-up investigations have demonstrated that some of these variations in data are a function of the different stereoisomeric forms of CAP. This paper discusses the implication of this research on method validation requirements and European Union legislation.

2654 related Products with: Chloramphenicol stereoisomers need to be distinguished: consequences observed from a proficiency test.

D-Alanine Benzyl Ester p- L-Alanine Benzyl Ester p- (2S)-2-Amino-benzenebutan 1,5-Anhydro-4,6-O-benzyli 1,5-Anhydro-4,6-O-benzyli (1R,3S,5R)-2-Azabicyclo[3 4-(Benzyloxy)-3-hydroxybe Homogenizer for 24 sample Mouse Anti-Cholera Toxin Sheep Anti-C. perfringens Her-2 IHC Bio-marker cont Rabbit Anti-Cholera Toxin

Related Pathways

paperclip

#28085100   2017/01/13 Save this To Up

Effects of Ambient Fine Particles PM2.5 on Human HaCaT Cells.

The current study was conducted to observe the effects of fine particulate matter (PM2.5) on human keratinocyte cell line (HaCaT) cells. The potential mechanism linking PM2.5 and skin was explored. HaCaT cells were cultured and then accessed in plate with PM2.5. Cell viability was tested by Cell Counting Kit-8. The mRNA and protein expression of Filaggrin, Loricrin, Involucrin, and Repetin were analyzed. The levels of Granulocyte-macrophage Colony Stimulating Factor, Thymic Stromal Lymphopoietin, Tumor Necrosis Factor-α, Interleukin-1α, and Interleukin-8 were detected in the supernatant of the HaCaT cell with enzyme-linked immunosorbent assay kits. Cell viability decreased with the increase in PM2.5. Compared with the control group, the protein expression of Filaggrin, Repetin, Involucrin, and Loricrin showed different expression patterns in PM2.5 treatment groups. The level of Tumor Necrosis Factor-α, Thymic Stromal Lymphopoietin, Interleukin-1α, and Interleukin-8 significantly increased in the cells treated with PM2.5. Ambient PM2.5 may increase the risk of eczema and other skin diseases. The relative mechanism may be associated with the impairment of the skin barrier and the elevation of inflammatory responses.

1549 related Products with: Effects of Ambient Fine Particles PM2.5 on Human HaCaT Cells.

Epidermal Growth Factor ( Epidermal Growth Factor ( Rabbit Anti-Human Red Blo ACTH [D Arg8](4 10)(Human Angiotensin II [Lys0](Hum Anti C Reactive Protein A Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon Anti Human AGO3, Monoclon Epidermal Growth Factor ( Epidermal Growth Factor (

Related Pathways

paperclip

#28077800   2017/01/12 Save this To Up

Oxidative stress induced by Se-deficient high-energy diet implicates neutrophil dysfunction via Nrf2 pathway suppression in swine.

The mechanism of the interaction between Se deficiency and high energy remains limited. The aim of the current study was to identify whether Se-deficient, high-energy diet can induce oxidative stress, and downregulate the Nrf2 pathway and phagocytic dysfunction of neutrophils. We detected the phagocytic activity, ROS production, protein levels of Nrf2 and Nrf2 downstream target genes, and the mRNA levels of 25 selenoproteins, heat shock proteins, and cytokines in neutrophils. Cytokine ELISA kits were used to measure the serum cytokines. The concentration of ROS was elevated (P < 0.05) in obese swine fed on a low Se diet (less than 0.03 mg/kg Se) compared to control swine. The protein levels of Nrf2 and its downstream target genes were depressed during Se deficiency and high-energy intake. The mRNA levels of 16 selenoproteins were significantly decreased (P < 0.05) in the Se-deficient group and Se-deficient, high-energy group compared to the control group. However, the mRNA levels of 13 selenoproteins in peripheral blood neutrophils were upregulated in high energy group, except TrxR1, SelI and SepW. In summary, these data indicated that a Se-deficient, high-energy diet inhibits the Nrf2 pathway and its regulation of oxidative stress, and prompted a pleiotropic mechanism that suppresses phagocytosis.

1572 related Products with: Oxidative stress induced by Se-deficient high-energy diet implicates neutrophil dysfunction via Nrf2 pathway suppression in swine.

Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon Anti AGO2 Human, Monoclon Anti AGO2 Mouse, Monoclon OXI TEK (Oxidative Stress Human Epstein-Barr Virus Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu Jurkat Cell Extract (Indu anti H inh human blood an Mouse Epstein-Barr Virus

Related Pathways

paperclip

#28071043   2017/01/10 Save this To Up

Decreased adipose tissue zinc content is associated with metabolic parameters in high fat fed Wistar rats.

Limited data on adipose tissue zinc content in obesity exist. At the same time, the association between adipose tissue zinc content and metabolic parameters in dietary-induced obesity is poorly studied. Therefore, the primary objective of this study is to assess adipose tissue zinc content and its association  with morphometric parameters, adipokine spectrum, proinflammatory cytokines, and apolipoprotein profile in high fat fed Wistar rats.

1878 related Products with: Decreased adipose tissue zinc content is associated with metabolic parameters in high fat fed Wistar rats.

High density breast invas High density breast cance High density (188 cases 2 High density (188 cases 2 Colon cancer high density High density colon cancer High density cervix cance High density cervical can High density esophageal c High density kidney cance High density non small ce High density Lung cancer

Related Pathways

paperclip

#28071012   2017/01/10 Save this To Up

Phenylketonuria is not a risk factor for changes of inflammation status as assessed by interleukin 6 and interleukin 8 concentrations.

High oxidative stress and a reduced potential for free radical scavenging in phenylketonuria (PKU) patients, a phenomenon confirmed in a few studies, may lead to systemic chronic inflammation. The aim of this study was to compare the inflammation status, as assessed by interleukin 6 and interleukin 8 concentrations, in patients with PKU and in healthy controls.

2419 related Products with: Phenylketonuria is not a risk factor for changes of inflammation status as assessed by interleukin 6 and interleukin 8 concentrations.

Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti interleukin 17 receptor C 4-O-Acetyl-2,5-anhydro-1, AST-1306 C24H18ClFN4O2 CA (3R,4S,5R,6S)-1-Aza-4-hyd Rabbit Anti-factor VIII(F Rabbit Anti-factor VIII(F Rabbit Anti-Eukaryotic as Rabbit Anti-Factor VII Po Rabbit Anti-ASM Acid sphi Rabbit Anti-D.Aspartic ac

Related Pathways

paperclip

#28070308   2017/01/10 Save this To Up

The seroprevalence of Mycobacterium avium subspecies paratuberculosis in dairy cattle in Xinjiang, Northwest China.

Mycobacterium avium subspecies paratuberculosis (MAP) causes chronic, wasting, and progressive enteritis in cattle, bringing significant economic losses in livestock industries. MAP has spread worldwide mainly due to movement of animals. The objective of this study was to determine the MAP seroprevalence in cattle in the Xinjiang Uygur Autonomous Region, Northwest China, and evaluate the difference between intensive farming herds (cattle number in a herd is more than 200, and the cattle cannot have access to pasture) and free-range herds (the cattle are bred by individual households, a herd is defined as the cattle are bred in a village or town in this study).

1947 related Products with: The seroprevalence of Mycobacterium avium subspecies paratuberculosis in dairy cattle in Xinjiang, Northwest China.

Thermal Shaker with cooli FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu FDA Standard Frozen Tissu Multiple organ tumor tiss MultiGene Gradient therm Interleukin-34 IL34 (N-t Interleukin-34 IL34 anti Sterile filtered goat se

Related Pathways

  •  
  • No related Items
paperclip

#28068681   2017/01/09 Save this To Up

Hypoxia and Hyperoxia Affect Serum Angiogenic Regulators in T2DM Men during Cycling.

Exercise-induced transient increases in pro-angiogenic regulators can promote angiogenesis.This pilot study aims to analyze the potential of exercise to positively affect angiogenic regulators in patients with type 2 diabetes mellitus (T2DM), who often exhibit abnormal angiogenesis, under different environmental conditions. 9 overweight/obese men with uncomplicated T2DM (8 took anti-diabetic drugs) performed submaximal cycling for 40 min in normoxia (≈21 vol%O2), hypoxia (≈14 vol%O2) and during alternating hypoxia/hyperoxia (≈14 vol%O2/≈30 vol%O2, 5-min intervals) (3×3 crossover design). Serum pro-angiogenic vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2, MMP-9 and anti-angiogenic endostatin were quantified using enzyme-linked immunosorbent assay (ELISA) kits. Non-parametric statistical tests (Wilcoxon, Friedman analysis of variance) were applied. VEGF increased significantly from pre- to post-exercise with hypoxia and hypoxia/hyperoxia. MMP-2 increased significantly in all experimental runs, while MMP-9 only increased significantly with hypoxia and hypoxia/hyperoxia. Endostatin increased significantly with normoxia and hypoxia. However, the magnitude of changes did not differ significantly between conditions. Capillary blood lactate was significantly lower following cycling with hypoxia/hyperoxia than with hypoxia alone. Although differences in subjective ratings of perceived exertion failed significance, 7 subjects were less exerted with hypoxia/hyperoxia than with hypoxia. Submaximal cycling with hypoxia or alternating hypoxia/hyperoxia may induce a more reliable up-regulation of pro-angiogenic regulators compared with normoxia, while hypoxia/hyperoxia may be better tolerated than hypoxia alone.

2474 related Products with: Hypoxia and Hyperoxia Affect Serum Angiogenic Regulators in T2DM Men during Cycling.

Sterile filtered goat se Sterile filtered goat se Sterile filtered mouse s Sterile filtered rat ser Bovine Androstenedione,AS Human interleukin 2(IL-2) Bovine prolactin-induced Innovative Grade™ Canin Innovative Grade™ Guine Innovative Grade™ Mouse Rabbit Serum US Origin In Rabbit Serum US Origin In

Related Pathways

paperclip

#28061872   2017/01/07 Save this To Up

Amoebic liver abscess in northern Sri Lanka: first report of immunological and molecular confirmation of aetiology.

Since 1985, amoebic liver abscess (ALA) has been a public health problem in northern Sri Lanka. Clinicians arrive at a diagnosis based on clinical and ultrasonographic findings, which cannot differentiate pyogenic liver abscess (PLA) from ALA. As the treatment and outcome of the ALA and PLA differs, determining the etiological agent is crucial.

1742 related Products with: Amoebic liver abscess in northern Sri Lanka: first report of immunological and molecular confirmation of aetiology.

Alkaline Phospatase (ALP) GLP 2 ELISA Kit, Rat Prog Leptin ELISA Kit, Rat Lep Zearalenone Mycotoxins EL Liver disease spectrum ti Liver carcinoma and norma Liver carcinoma and norma Liver carcinoma and norma Colon cancer, metastasize GI cancer (esophageal, ga Liver cancer antibody scr Liver cancer tissue array

Related Pathways

  •  
  • No related Items
paperclip

#28045894   2017/01/03 Save this To Up

Investigation of a Novel Hepatitis B Virus Surface Antigen (HBsAg) Escape Mutant Affecting Immunogenicity.

Mutation in the hepatitis B virus surface antigen (HBsAg) may affect the efficiency of diagnostic immunoassays or success of vaccinations using HBsAg. Thus, antigenicity and immunogenicity analyses of the mutated HBsAg are necessary to develop novel diagnostic tools and efficient vaccinations. Here, the in vitro antigenicity of three wild-type HBsAg open reading frames (ORFs) (adr4, W1S [subtype adr] and W3S [subtype adr]) isolated from clinically infected patients and nineteen synthesized single/double/multiple amino acid-substituted mutants were tested with commercial ELISA kits. Immunofluorescence staining of transfected cells and Western blot analysis confirmed that these ORFs were expressed at comparable levels in HEK-293 cells. W1S and adr4 were clearly detected, whereas W3S could not be detected. Using the same commercial immunoassay kit, we found that the single mutants, K120P and D123T, were marginally reactive, whereas W3S-aW1S and the double mutant, K120P/D123T, exhibited antigenicity roughly equivalent to the wild-type wako1S. On the other hand, the single mutants of W1S, P120K and T123D, significantly impaired the reactivity, while W1S-aW3S and the double mutant of W1S, P120K/T123D, resulted in a complete loss of antigenicity. In addition, ELISA revealed reduced HBs antigenicity of two mutants, W1S N146G and W1S Q129R/G145R. These commercial ELISA-based antigenic reactivities of HBsAg were also strongly correlated with the predicted Ai alterations of affected amino acids due to the specific mutation. In conclusion, this study showed for the first time that lysine (K120) and aspartate (D123) simultaneously affected HBsAg antigenicity, leading to diagnostic failure. These findings will improve diagnostic assays and vaccine development.

2962 related Products with: Investigation of a Novel Hepatitis B Virus Surface Antigen (HBsAg) Escape Mutant Affecting Immunogenicity.

Human Hepatitis B Surface Anti-Hepatitis B Surface Anti-Hepatitis B Surface HBV surface recombinant a HBV surface recombinant a HBV surface recombinant a Tick born encephalitis vi anti HBsAg surface antige Rabbit Anti-Polyprotein(H Rabbit Anti-Hepatitis C V Rabbit Anti-Hepatitis C V Recombinant Chikungunya M

Related Pathways

paperclip

#28043543   2017/01/03 Save this To Up

Investigation of urine lipoarabinomannan in human immunodeficiency virus patients with or without coinfection with Tuberculosis in Iran.

Tuberculosis (TB) remains the leading cause of AIDS-related deaths among adults in countries with resource limitations. The emergence of the Xpert MTB/RIF rapid molecular assay and its subsequent World Health Organization endorsement in 2010 transformed the TB-diagnostic landscape. Xpert provided diagnostic accuracy that was far superior to that of the sputum-smear microscopy test previously used. The detection of mycobacterial lipoarabinomannan (LAM) antigen in urine has emerged as a potential point-of-care test for TB. LAM antigen is a lipopolysaccharide present in mycobacterial cell walls which is released from metabolically active or degenerating bacterial cells and appears to be present only in people with active TB. Urine-based testing has advantages over sputum-based testing because urine is easy to collect and store and lacks the infection control risks associated with sputum collection. A previously study reported that urinary-LAM testing is a rapid, low-cost, ante-mortem diagnosis for human immunodeficiency virus (HIV)-associated TB. The objective of this study was to investigate the levels of LAM in HIV patients referred to the Mashih Daneshvari Hospital Tehran, Iran.

2478 related Products with: Investigation of urine lipoarabinomannan in human immunodeficiency virus patients with or without coinfection with Tuberculosis in Iran.

Human Epstein-Barr Virus Goat Anti-Human ORAI1 CRA Multiple organ tumor tiss Multiple organ tumor tiss Multiple organ tumor tiss Frozen multiple human org FIV Core Ag, recombinant Syringe pump can be contr Anti AGO2 Human, Monoclon Anti AGO2 Human, Monoclon CELLKINES Natural Human I Human Interleukin-4 IL-4

Related Pathways