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#27341611   2016/6/24 Save this To Up

Conceptual processing is referenced to the experienced location of the self, not to the location of the physical body.

We tested whether an experimentally induced change in the experienced location of the self by means of the Full Body Illusion (FBI) would impact high-level cognitive processing. In an implicit association task, participants saw a landscape where words, conveying spatial ("Near", "Far") or social ("Us", "Them") semantic distance appeared either in a proximal or distal position. Words' "semantic" distance was congruent or incongruent to the words' spatial position. Participants were asked to classify words' spatial position. Implicit association judgements were intermingled with sessions of visuo-tactile stimulation, during which participants were touched on their back, while viewing an avatar in the distal position being touched either synchronously or asynchronously. In a control experiment, participants performed the same task while observing a neutral object being touched, as a control. When subjects self-identified with the avatar presented at the distal position, the facilitation effect of the words' spatial-semantic congruency disappeared. The congruency effect did not disappear in case of asynchronous stimulation as well as in the control experiment with the object, where no change in self-identification was observed. These results demonstrate that conceptual processing is not referenced to the location of the physical body, but to the experienced location of the self.

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#27341567   2016/6/24 Save this To Up

High-dose bee venom exposure induces similar tolerogenic B cell responses in patients and healthy beekeepers.

The involvement of B cells in allergen tolerance induction remains largely unexplored. This study investigates the role of B cells in allergen tolerance induction to high-dose allergen exposure, and to compare B cell responses between allergic patients receiving allergen immunotherapy (AIT) and naturally exposed healthy beekeepers before and during the beekeeping season.

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#27341529   2016/6/24 Save this To Up

Associations Between Delirium and Preoperative Cerebrospinal Fluid C-Reactive Protein, Interleukin-6, and Interleukin-6 Receptor in Individuals with Acute Hip Fracture.

To examine whether delirium in individuals with hip fracture is associated with high C-reactive protein (CRP), interleukin-6 (IL-6), and soluble IL-6 receptor (sIL-6R) levels in the cerebrospinal fluid (CSF).

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#27341419   2016/6/24 Save this To Up

Production and Characterization of Monoclonal Antibody Broadly Recognizing Cry1 Toxins by Use of Designed Polypeptide as Hapten.

In this study, by use of synthesized polypeptides as haptens, a monoclonal antibody with broad recognition against seven major Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, Cry1E, and Cry1F) has been produced and characterized. First, by comparing the three-dimensional structures of seven Cry1 toxins, analyzing the conserved sequences, and considering the antigenicity and hydrophilicity, three polypeptides (T1, T2, and T3) have been chosen and coupled to keyhole limpet hemocyanin as immunogens for the generic monoclonal antibody (Mab) generation. Thereafter, a double antibody sandwich enzyme-linked immunosorbent assay method (DAS-ELISA) was developed for simultaneous determination of seven Cry1 toxins. The results revealed that the haptens T1, T2, and T3 had different effects in the production of antibodies. Among them, the obtained Mab (strain 2D3) generated by T2 can recognize seven Cry1 toxins simultaneously. Equilibrium dissociation constant (KD) values for seven Cry1 toxins with Mab 2D3 were 1.198 × 10(-8) M for Cry1Aa, 2.197 × 10(-8) M for Cry1Ab, 1.367 × 10(-8) M for Cry1Ac, 2.092 × 10(-8) M for Cry1B, 5.177 × 10(-8) M for Cry1C, 4.016 × 10(-8) M for Cry1E, and 3.497 × 10(-8) M for Cry1F. For 2D3-based DAS-ELISA, the limits of detection (LOD) and limits of quantification (LOQ) can reach 15 and 30 ng·mL(-1) for each Cry1 toxin, respectively. Our study is the first report of a broadly specific immunoassay for multidetermination of seven major Cry1 toxins, and it will provide a new idea and technical routes for development of multidetermination immunoassays.

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#27341222   2016/6/24 Save this To Up

Prospects for Multiband Gravitational-Wave Astronomy after GW150914.

The black hole binary (BHB) coalescence rates inferred from the Advanced LIGO detection of GW150914 imply an unexpectedly loud gravitational-wave (GW) sky at millihertz frequencies accessible to the Evolved Laser Interferometer Space Antenna (eLISA), with several outstanding consequences. First, up to thousands of BHBs will be individually resolvable by eLISA; second, millions of nonresolvable BHBs will build a confusion noise detectable with a signal-to-noise ratio of a few to hundreds; third-and perhaps most importantly-up to hundreds of BHBs individually resolvable by eLISA will coalesce in the Advanced LIGO band within 10 y. eLISA observations will tell Advanced LIGO and all electromagnetic probes weeks in advance when and where these BHB coalescences will occur, with uncertainties of <10  s and <1  deg^{2}. This will allow the prepointing of telescopes to realize coincident GW and multiwavelength electromagnetic observations of BHB mergers. Time coincidence is critical, because a prompt emission associated to a BHB merger will likely have a duration comparable to the dynamical time scale of the systems and is possible only with low-frequency GW alerts.

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#27341090   2016/6/24 Save this To Up

Relationship Between Dynamic Changes in Expression of IL-17/IL-23 in Lacrimal Gland and Ocular Surface Lesions in Ovariectomized Mice.

An ovariectomized mouse model was constructed to observe the dynamic effects of hormone changes on the expression of interleukin (IL)-17A and IL-23 in the lacrimal glands.

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#27340743   2016/6/24 Save this To Up

Convergent solid-phase synthesis of macromolecular MUC1 models truly mimicking serum glycoprotein biomarkers of interstitial lung diseases.

Synthetic macromolecular MUC1 glycopeptides unraveled mo-lecular mechanisms in antibody recognition of the disease-specific epitopes. We established a novel synthetic strategy for MUC1 tandem repeats having complex O-glycosylation states at each repeating units based on the convergent solid-phase frag-ment condensation under microwave irradiation. We accom-plished the synthesis of 77-mer amino acids MUC1 glycopep-tides (MW=12,759) having three major antigenic O-glycoforms, Tn, core 1 (T), and core 2 structures, at 10 designated positions out of 19 potential O-glycosylation sites. It was demonstrated that the macromolecular MUC1 glycopeptide displaying the essential glycopeptidic neo-epitope, Pro-Asp-Thr(sialyl-T)-Arg-Pro-Ala-Pro, at two different tandem repeats is an excellent serum MUC1 model showing an ideal stoichiometric binding with anti-KL6/MUC1 antibody in the sandwich ELISA to quan-tify human serum KL6/MUC1 levels as a critical biomarker of the interstitial lung diseases.

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#27340684   2016/06/24 Save this To Up

Proinflammatory cytokines and matrix metalloproteinases in CSF of patients with VZV vasculopathy.

To determine the levels of proinflammatory cytokines and matrix metalloproteinases (MMPs) in the CSF of patients with virologically verified varicella zoster virus (VZV) vasculopathy.

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#27340671   2016/06/24 Save this To Up

First Report of Bovine Leukemia Virus Infection in Yaks (Bos mutus) in China.

Enzootic bovine leukosis (EBL) is a chronic lymphosarcoma disease of cattle caused by bovine leukemia virus (BLV). No information is available concerning the epidemiology of BLV infection in yaks (Bos mutus). One thousand five hundred and eighty-four serum samples from 610 black yaks and 974 white yaks from Gansu province, northwest China, were collected between April 2013 and March 2014 and tested for BLV antibodies using a commercially available ELISA kit. The overall BLV seroprevalence in yaks was 21.09% (334/1584), with 24.26% (148/610) black yaks and 19.10% (186/974) white yaks yielding positive results. Risk factor analysis indicated that with the exception of breed (OR = 1.36, 95% CI = 1.06-1.73, P < 0.05), the age, region, gender, farm, and the numbers of pregnancies were not considered as risk factors for the presence of BLV in yaks included in this study. This is the first report of BLV infection in yaks in China, which provides information for controlling BLV infection in yaks.

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#27340619   2016/06/24 Save this To Up

Improvement of mesenchymal stem cell differentiation into the endoderm lineage by four step sequential method in biocompatible biomaterial.

The goal of the study described here, was to investigate the potential of umbilical cord derived mesenchymal stem cell (UC-MSCs) into hepatocyte like cells in a sequential 2D and 3D differentiation protocols as alternative therapy.

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