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#25412170   2014/11/21 Save this To Up

Evaluation of Dengue NS1 Antigen Rapid Tests and ELISA Kits Using Clinical Samples.

Early diagnosis of dengue virus (DENV) infection can improve clinical outcomes by ensuring close follow-up, initiating appropriate supportive therapies and raising awareness to the potential of hemorrhage or shock. Non-structural glycoprotein-1 (NS1) has proven to be a useful biomarker for early diagnosis of dengue. A number of rapid diagnostic tests (RDTs) and enzyme-linked immunosorbent assays (ELISAs) targeting NS1 antigen (Ag) are now commercially available. Here we evaluated these tests using a well-characterized panel of clinical samples to determine their effectiveness for early diagnosis.

1927 related Products with: Evaluation of Dengue NS1 Antigen Rapid Tests and ELISA Kits Using Clinical Samples.

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#25409967   2014/11/21 Save this To Up

Effect of the ingestion of the palm oil and glutamine in serum levels of GLP-1, PYY and glycemia in diabetes mellitus type 2 patients submitted to metabolic surgery.

Incretins are hormones produced by the intestine and can stimulate the secretion of insulin, helping to diminish the post-prandial glycemia. The administration of an emulsion of palm oil can help in the maintenance of the weight, and can increase circulating incretins levels. Glutamine increases the concentration of incretins in diabetic people. Both can help in metabolic syndrome.

1367 related Products with: Effect of the ingestion of the palm oil and glutamine in serum levels of GLP-1, PYY and glycemia in diabetes mellitus type 2 patients submitted to metabolic surgery.

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#25396058   2014/11/14 Save this To Up

Clinical phenotype associations with various types of anti-dsDNA antibodies in patients with recent onset of rheumatic symptoms. Results from a multicentre observational study.

Despite anti-dsDNA antibodies constitute a wide range of specificities, they are considered as the hallmark for systemic lupus erythematosus (SLE).

1087 related Products with: Clinical phenotype associations with various types of anti-dsDNA antibodies in patients with recent onset of rheumatic symptoms. Results from a multicentre observational study.

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#25394190   2014/11/13 Save this To Up

Performance of Rapid HIV Testing by Lay Counsellors in the Field during the Behavioural and Biological Surveillance Survey among Female Sex Workers and Men who have Sex with Men in Botswana.

The study assessed the performance of rapid HIV testing with whole blood using KHB HIV (1+2) and Uni-Gold HIV test kits by trained and certified lay counsellors, offered to female sex workers (FSW) and men who have sex with men (MSM) during the 2012 survey fieldwork. The results of rapid HIV testing were compared to enzyme-linked immunosorbent assay (ELISA) testing carried out in a parallel algorithm at the HIV Reference Laboratory. The sensitivity and the specificity of rapid HIV testing were high for MSM and FSW, with 98.1% and 100%, and 98.2% and 98.5% respectively. Misclassifications occurred with rapid testing.

2715 related Products with: Performance of Rapid HIV Testing by Lay Counsellors in the Field during the Behavioural and Biological Surveillance Survey among Female Sex Workers and Men who have Sex with Men in Botswana.

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#25388913   2014/11/12 Save this To Up

High seroprevalence of echinococossis, schistosomiasis and toxoplasmosis among the populations in Babati and Monduli districts, Tanzania.

BackgroundThe neglected tropical diseases, echinococcosis, schistosomiasis and toxoplasmosis are all globally widespread zoonotic diseases with potentially harmful consequences. There is very limited data available on the prevalence of these infections, except for schistosmiasis, in underdeveloped countries. This study aimed to determine the seroprevalence of Echinococcus multilocularis, Schistosoma mansoni, and Toxoplasma gondii antibodies in populations from the Monduli and Babati districts in Tanzania.MethodsA total of 345 blood samples were collected from 160 and 185 randomly selected households from Babati and Monduli districts, Tanzania between February and May of 2012 and analyzed them using the enzyme linked immunosorbent assay. The antibodies were determined using the NovaLisa® Toxoplasma gondii IgG, NovaLisa® Schistosoma Mansoni IgG, NovaLisa® Echinococcus IgG and NovaLisa® Toxoplasma gondii IgM kits (Novatec, Germany).ResultsThe seropositivity estimated for E. multilocularis, S. mansoni, and T. gondii IgG was 11.3% (95% confidence interval (CI): 7.96 - 14.6), 51.3% (95% CI: 46.0 - 56.5), and 57.68% (95% CI: 52.5 - 62.9), respectively. The seropositivity for T. gondii IgM was 11.3% (95% CI: 7.96 - 14.6). Living in the Monduli district was found to be the main risk factor for IgG seropositivity for both schistosomiasis (OR =1.94; 95% CI: 1.23 - 3.08; p =0.005) and toxoplasmosis (OR =2.09; 95% CI: 1.31-3.33; p =0.002).ConclusionsThese results suggest that restricting disease transmission, implementing control measures, and introducing training projects to increase public awareness are imperative, particularly for the Monduli district.

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#25385391   2014/11/11 Save this To Up

Assessment of a Test for the Screening and Diagnosis of Celiac Disease.

Celiac disease (CD) is an immune-mediated intolerance to dietary gluten, affecting genetically predisposed individuals. ELISA-based serological tests help to decide if further duodenal biopsy is necessary, for this the diagnostic kits have to be accurate, specific, and sensible. In this study, we investigate the performance of an ELISA that uses the purified cross-linked complex of tissue transglutaminase and gliadin, referred as the "neoepitope" (AESKULISA® tTG New Generation), as antigen.

1238 related Products with: Assessment of a Test for the Screening and Diagnosis of Celiac Disease.

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#25380625   2014/11/8 Save this To Up

Circulating levels of apelin, glucagon-like peptide and visfatin in hypercholesterolemic-hyperhomocysteinemic guinea-pigs: their relation with NO metabolism.

The aim of this study was to determine the levels of regulatory peptides apelin, glucagon-like peptide (GLP-1) and visfatin in hypercholesterolemic and hyperhomocysteinemic state and to examine their relation with nitric oxide (NO) metabolism. 32 Male guinea pigs were divided into four groups and each group was fed as follows: (a) commercial chow, (b) cholesterol (chol)-rich diet, (c) methionine (meth)-rich diet, and (d) chol + meth-rich diet. Blood samples were drawn at the end of 10 weeks, and abdominal aorta was dissected for histopathological examination. Serum insulin, GLP-1, apelin, visfatin, and nitrotyrosine concentrations were measured by the manufacturer's kits based on ELISA; asymmetric dimethylarginine (ADMA) and arginine levels were measured by the high performance liquid chromatography. Homocysteine level was measured by the chemiluminescence immunoassay; glucose, total chol and triglyceride levels were measured by the autoanalyzer. The microscopic examination of aorta indicated varying degrees of vascular disturbance in chol- and chol + meth-fed groups. High levels of chol and homocysteine, accompanied with significantly low levels of apelin and GLP-1 were detected in the plasma. Visfatin, ADMA, and nitrotyrosine levels both in chol- and chol + meth-fed groups were significantly higher than those in control animals, whereas arginine and arginine/ADMA ratio were lower. This study indicated that circulating levels of apelin, GLP-1, and visfatin are markedly altered during the development of atherosclerotic changes in close association with chol, homocysteine, NO, and ADMA levels. The measurements of these peptides in serum may help for the diagnosis and follow-up of vascular dysfunction.

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#25379480   2014/11/07 Save this To Up

Study of serum interleukin (IL) 18 and IL-6 levels in relation with the clinical disease severity in chronic idiopathic urticaria patients of Kashmir (North India).

Chronic urticaria is termed as idiopathic if there is an absence of any identifiable causes of mast cell and basophil degranulation. Various cytokines have been found to be involved in inflammatory processes associated with chronic idiopathic urticaria, including interleukin (IL) 18 and IL-6.

2323 related Products with: Study of serum interleukin (IL) 18 and IL-6 levels in relation with the clinical disease severity in chronic idiopathic urticaria patients of Kashmir (North India).

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#25378771   2014/11/07 Save this To Up

Usefulness of enzyme immunoassay (EIA) for screening of anti HIV antibodies in urinary specimens: A comparative analysis.

Standard HIV testing is done using serum or plasma. FDA approved ELISA to screen urine for IgG antibodies to HIV-1 in 1996. It is a simple, noninvasive test and is appropriate for developing countries where health care personnel may not be professionally trained or where clean needles for drawing blood may not always be available.

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HIV1 integrase antibody, HIV 1 intergase antigen. MONOBODIES (Monoclonal An MONOBODIES (Monoclonal An MONOBODIES (Monoclonal An MONOBODIES (Monoclonal An MONOBODIES (Monoclonal An MONOBODIES (Monoclonal An MONOBODIES (Monoclonal An HIV1 gp41 antibody, Monoc HIV2 p26 antibody, Monocl HIV1 Nef antibody, Monocl

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#25367907   2014/11/4 Save this To Up

Cationic liposomal-sodium stibogluconate (SSG): A potent therapeutic tool for the treatment of SSG-sensitive and -resistant Leishmania donovani infection.

Pentavalent antimonials have been the first line treatment of leishmaniasis for decades. However, development of resistance to sodium stibogluconate (SSG) has limited its use especially for treating visceral leishmaniasis (VL). The present work aims at optimizing a cationic liposomal formulation of SSG for the treatment of both SSG-sensitive (AG83) and resistant (GE1F8R and CK1R) Leishmania donovani infection. Parasite killing was determined by MTT assay and microscopic counting of Giemsa-stained macrophages. Macrophage uptake studies were carried out by confocal imaging. Parasite-liposome interaction was visualized through transmission electron microscopy. Toxicity tests were performed using assay kits. Organ parasite burden was determined by microscopic counting and limiting dilution assay. Cytokines were measured by ELISA and flow cytometry. Although all cationic liposomes studied demonstrated leishmanicidal activity, phosphatidylcholine-dimethyldioctadecylammonium bromide (PC-DDAB) vesicles were most effective followed by PC-stearylamine (PC-SA) liposomes. Since entrapment of SSG in PC-DDAB demonstrated enhanced ultrastructural alterations in promastigotes, PC-DDAB-SSG vesicles were further investigated in vitro and in vivo. PC-DDAB-SSG could effectively alleviate SSG-sensitive and -resistant L. donovani infection in the liver, spleen and bone marrow of BALB/c mice at a dose of SSG (3 mg/kg body weight) not reported earlier. The parasiticidal activity of these vesicles was attributed to better interaction with the parasite membrane resulting in direct killing, and generation of a strong host-protective environment requiring very low dose of SSG for effective cure.

1327 related Products with: Cationic liposomal-sodium stibogluconate (SSG): A potent therapeutic tool for the treatment of SSG-sensitive and -resistant Leishmania donovani infection.

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