Search results for: miRNASelect pEPhsamir874 Expression Vector
#38630231 // To Up
Live Imaging of Migrating Neurons and Glial Progenitors Visualized by in Utero Electroporation.
During cortical development, both neurons and glial cells are generated in the germinal zone near the lateral ventricle, migrate in the correct direction, and settle in their appropriate locations. This developmental process can be clearly visualized by introducing fluorescent protein-expression vectors via in utero electroporation. In this chapter, we describe labeling methods for migrating neurons and glial progenitors, as well as methods for slice culture, and time-lapse imaging.Masashi Nishikawa, Koh-Ichi Nagata, Hidenori Tabata
2351 related Products with: Live Imaging of Migrating Neurons and Glial Progenitors Visualized by in Utero Electroporation.
900 testsRelated Pathways
#38629194 // To Up
Chinese cherry CpMYB44-CpSPDS2 module regulates spermidine content and florescence in tobacco.
The flower bud differentiation plays a crucial role in cherry yield and quality. In a preliminary study, we revealed the promotion of spermidine (Spd) in bud differentiation and quality. However, the molecular mechanism underlying Spd regulating cherry bud differentiation remains unclear. To address this research gap, we cloned CpSPDS2, a gene that encodes Spd synthase and is highly expressed in whole flowers and pistils of the Chinese cherry (cv. 'Manaohong'). Furthermore, an overexpression vector with this gene was constructed to transform tobacco plants. The findings demonstrated that transgenic lines exhibited higher Spd content, an earlier flowering time by 6 d, and more lateral buds and flowers than wild-type lines. Additionally, yeast one-hybrid assays and two-luciferase experiments confirmed that the R2R3-MYB transcription factor (CpMYB44) directly binds to and activates the CpSPDS2 promoter transcription. It is indicated that CpMYB44 promotes Spd accumulation via regulating CpSPDS2 expression, thus accelerating the flower growth. This research provides a basis for resolving the molecular mechanism of CpSPDS2 involved in cherry bud differentiation.Hong Deng, Qiandong Hou, Zhuang Wen, Runrun Yu, Xuejiao Cao, Chunqiong Shang, Xiaowei Cai, Guang Qiao
2841 related Products with: Chinese cherry CpMYB44-CpSPDS2 module regulates spermidine content and florescence in tobacco.
280025 1600100 μg1 mg 1 G100 μg8 Sample Kit100 μg100Related Pathways
#38627969 2024/04/15 To Up
Evolution of the clinical-stage hyperactive TcBuster transposase as a platform for robust non-viral production of adoptive cellular therapies.
Cellular therapies for the treatment of human diseases, such as chimeric antigen receptor (CAR) T and NK cells have shown remarkable clinical efficacy in treating hematological malignancies, however current methods mainly utilize viral vectors which are limited by their cargo size capacities, high cost, and long timelines for production of clinical reagent. Delivery of genetic cargo via DNA transposon engineering is a more timely and cost-effective approach, yet has been held back by less efficient integration rates. Here, we report the development of a novel hyperactive TcBuster (TcB-M) transposase engineered through structure guided and in vitro evolution approaches that achieves high-efficiency integration of large, multicistronic CAR-expression cassettes in primary human cells. Our proof of principle TcB-M engineering of CAR-NK and CAR-T cells show low integrated vector copy number, a safe insertion site profile, robust in vitro function, and improves survival in a Burkitt lymphoma xenograft model in vivo. Overall, TcB-M is a versatile, safe, efficient and open-source option for the rapid manufacture and preclinical testing of primary human immune cell therapies through delivery of multicistronic large cargo via transposition.Joseph G Skeate, Emily J Pomeroy, Nicholas J Slipek, Bryan J Jones, Bryce J Wick, Jae-Woong Chang, Walker S Lahr, Erin M Stelljes, Xiaobai Patrinostro, Blake Barnes, Trevor Zarecki, Joshua B Krueger, Jacob E Bridge, Gabrielle M Robbins, Madeline D McCormick, John R Leerar, Kari T Wenzel, Kathlyn M Hornberger, Kirsti Walker, Dalton Smedley, David A Largaespada, Neil Otto, Beau R Webber, Branden S Moriarity
1254 related Products with: Evolution of the clinical-stage hyperactive TcBuster transposase as a platform for robust non-viral production of adoptive cellular therapies.
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#38626865 2024/04/14 To Up
Pathogen-associated molecular patterns (PAMPs) derived from Leishmania and bacteria increase gene expression of antimicrobial peptides and gut surface proteins in sand flies.
TBarbora Vomáčková Kykalová, Fabiana Sassù, Felipe Dutra Rêgo, Rodrigo Pedro Soares, Petr Volf, Erich Loza Telleria
2200 related Products with: Pathogen-associated molecular patterns (PAMPs) derived from Leishmania and bacteria increase gene expression of antimicrobial peptides and gut surface proteins in sand flies.
100ul300 units200ul1005mgRelated Pathways
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#38626731 2024/04/16 To Up
Forward problem of electrocardiography based on cardiac source vector orientations.
TReshma H, Vikas R Bhat, Anitha H
1854 related Products with: Forward problem of electrocardiography based on cardiac source vector orientations.
100ulOne 96-Well Microplate Ki100ug50ul 100ul 100ul 100ul 100ul 100ul 100ul 100ul 100ulRelated Pathways
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#38626531 2024/04/09 To Up
Overexpression of Slc22a18 facilitates fat accumulation in mice.
WTakashi Yamamoto, Yoko Iizuka, Kozue Izumi-Yamamoto, Midori Shirota, Nobuko Mori, Yoshikazu Tahara, Toshiro Fujita, Takanari Gotoda
2113 related Products with: Overexpression of Slc22a18 facilitates fat accumulation in mice.
3 inhibitors96 tests96tests100 100 μg100 ul5 mg100 μg250 mg50ug100ug LyophilizedRelated Pathways
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#38626311 2024/04/16 To Up
ATP13A3 variants promote pulmonary arterial hypertension by disrupting polyamine transport.
Potential loss-of-function variants of ATP13A3, the gene encoding a P5B-type transport ATPase of undefined function, were recently identified in pulmonary arterial hypertension (PAH) patients. ATP13A3 is implicated in polyamine transport but its function has not been fully elucidated. Here, we sought to determine the biological function of ATP13A3 in vascular endothelial cells and how PAH-associated variants may contribute to disease pathogenesis.Bin Liu, Mujahid Azfar, Ekaterina Legchenko, James A West, Shaun Martin, Chris Van den Haute, Veerle Baekelandt, John Wharton, Luke Howard, Martin R Wilkins, Peter Vangheluwe, Nicholas W Morrell, Paul D Upton
1576 related Products with: ATP13A3 variants promote pulmonary arterial hypertension by disrupting polyamine transport.
500 ml 1000 ml 100ug 500 ml 100ul 25UG 1000 ml 20 Bags of 25 Tubes/Unit5 x 200ul 500 mlRelated Pathways
#38626093 2024/04/16 To Up
Peak transgene expression after intramuscular immunization of mice with adenovirus 26-based vector vaccines correlates with transgene-specific adaptive immune responses.
NSonia Marquez-Martinez, Nadine Salisch, Jan Serroyen, Roland Zahn, Selina Khan
1393 related Products with: Peak transgene expression after intramuscular immunization of mice with adenovirus 26-based vector vaccines correlates with transgene-specific adaptive immune responses.
100 µLRelated Pathways
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#38626020 2024/04/16 To Up
Members of the paralogous gene family 12 from the Lyme disease agent Borrelia burgdorferi are non-specific DNA-binding proteins.
LKalvis Brangulis, Inara Akopjana, Laura Drunka, Sofija Matisone, Diana Zelencova-Gopejenko, Shapla Bhattacharya, Janis Bogans, Kaspars Tars
1545 related Products with: Members of the paralogous gene family 12 from the Lyme disease agent Borrelia burgdorferi are non-specific DNA-binding proteins.
100 UG1 mL100μl1mg1 mL100μl2 Pieces/Box101 mL100μl1 mg100μlRelated Pathways
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#38625980 2024/04/16 To Up
From an obliquely falling rod in a viscous fluid to the motion of suspended magnetic bead chains that are driven by a gradient magnetic field and that make an arbitrary angle with the magnetic force vector: A Stokes flow study.
IRobert J Deissler, Rose Al Helo, Robert Brown
1023 related Products with: From an obliquely falling rod in a viscous fluid to the motion of suspended magnetic bead chains that are driven by a gradient magnetic field and that make an arbitrary angle with the magnetic force vector: A Stokes flow study.
100.00 ul100 μg1 Set1 mg100ug LyophilizedRelated Pathways
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