Leptin, secreted from fatty cells, is known as a hormone controlling body fat by suppresssion of eating and increase of energy metabolism. It is reported, however, that expression of leptin gene in fatty tissue and concentration of leptin in blood are at high levels in obese people and model animals of obesity. Samples for serum and plasma : 312.5 ~ 20,000 pg/mL Samples for others than serum and plasma : 78.1 ~ 5,000 pg/mL Specific to rat Leptin, but only slightly cross-reactive to human Leptin.

Principle of the assay
This kit is a sandwich-format of ELISA with two antibodies specific to rat leptin. Monoclonal antibody to leptin is coated on the microplate well, which captures leptin in sample. Following reaction with HRPconjugated rabbit anti rat leptin antibody, forms HRP labeled antigenantibody complex on the well, which reports the signal of leptin amount in the sample by chromogenic reaction associated with
catalysis of hydrogen peroxide.

Dynamic range :
• Samples for serum and plasma : 312.5 ~ 20,000 pg/mL
• Samples for others than serum and plasma : 78.1 ~ 5,000 pg/mL

Cross-reactivity
Specific to rat Leptin, but only slightly cross-reactive to human Leptin.

Kit Contents (96 tests)
1. Antibody-coated Microtiter Plate 1 plate
(Anti Rat Leptin, monoclonal antibody)
2. Rat Leptin Standard 20 ng
3. HRP-conjugated Antibody, rabbit 6 mL
4. Chromogen (OPD Tablet) 2 tablets
5. Chromogen Diluent Solution 24 mL
6. Wash Stock Solution (20 x) 50 mL
7. Buffer A 20 mL
8. Buffer B 20 mL
9. Stop Solution (1mol/L H2SO4) 12 mL
10. Adhesive Plate Cover 2 covers