Full length recombinant mature FGF-1 (15.8 kDa, 140 amino acids) expressed in E. coli.FGF-1 (acidic FGF) is a member of the fibroblast growth factor (FGF) family, which binds heparin. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.

Applications

1. Use as a supplement in serum-free or reduced serum media for culture of mammalian cells.

2. Studies of the human FGF1 receptor, transmembrane signaling and protein phosphorylation

3. Western blotting control for anti-EGF antibodies

Specifications
Activity: The ED50 as determined by a cell proliferation assay using MTS assay kit (CellTiter 96, Promega) with Balb/c3T3 cells was < 10 pg/ml, corresponding to a specific activity of < 1 x 108 units/mg.
Purity: >98% as determined by SDS-PAGE (CBB staining)
Form: 1.0 mg/ml in PBS (10mM Na-phosphate, 150mM NaCl) pH7.2, 50% glycerol, filter-sterilyzed
Storage: -20 oC (for long-term storage, -70 oC)