DESCRIPTION

Fatty acids are aliphatic monocarboxylic acids that are ubiquitously found in animal or vegetable fat, oil and wax. Fatty Acids play important roles in cellular synthesis, energy metabolism and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome and Reye Syndrome. BioAssay Systems' method provides a simple, one-step and high-throughput assay for measuring free fatty acids. In this assay, free fatty acids are enzymatically converted to acyl- CoA and subsequently to H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the free fatty acid concentration in the sample.

KEY FEATURES

Sensitive. Use 10 μL samples. Linear detection range: colorimetric assay 7 - 1000 μM, fluorimetric assay 7 - 100 μM fatty acid. Convenient. Room temperature “mix-and-read” procedure can be readily automated for high-throughput assay of thousands of samples per day.

APPLICATIONS

Assays: free fatty acids in biological samples such as serum, plasma, urine, saliva, milk, cell cultures and in food, agriculture products.
Drug Discovery/Pharmacology: effects of drugs on free fatty acid metabolism.

KIT CONTENTS

Assay Buffer: 20 mL D y e R e agent: 120 μL
Enzyme A: 120 μL Enzyme B: 120 μL
CoSubstrate: 120 μL Standard: 1 mL 1 mM palmitic acid
 

Storage conditions. The kit is shipped on ice. Store all components at -20°C. Shelf life of three months after receipt.
 

Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.