Search results for: (2S,3S)-3-Amino-2-methyl-4-oxoazetidine-1-sulphonic Acid C4H8N2O4S CAS: 80082-65-1
#38647893 
2022/07/30
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Production of free fatty acids from various carbon sources by Ogataea polymorpha.
EYunxia Li, XiaoXin Zhai, Wei Yu, Dao Feng, Aamer Ali Shah, Jiaoqi Gao, Yongjin J Zhou
2240 related Products with: Production of free fatty acids from various carbon sources by Ogataea polymorpha.
1,000 tests100tests1001KG500gm1kg100gm500g50gm
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#38647822 2022/05/26
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Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.
�Peng Cai, Yunxia Li, Xiaoxin Zhai, Lun Yao, Xiaojun Ma, Lingyun Jia, Yongjin J Zhou
1881 related Products with: Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.
1 mg100 µg1 mL100ug100ug6 ml100ug Lyophilized500 1mg2 mg100.00 ug0.5 mg
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#38647771 2022/10/22
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Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.
IYunfei Zhu, Gaofan Zheng, Xiujuan Xin, Junying Ma, Jianhua Ju, Faliang An
2529 related Products with: Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.
1 mg100μg0.1ml (1mg/ml)500 500 500 μg100ug100ug Lyophilized100ug1 mL100ug Lyophilized100μg
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#38647589 2022/05/18
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From formic acid to single-cell protein: genome-scale revealing the metabolic network of Paracoccus communis MA5.
WSheng Tong, Lizhi Zhao, Daling Zhu, Wuxi Chen, Limei Chen, Demao Li
1951 related Products with: From formic acid to single-cell protein: genome-scale revealing the metabolic network of Paracoccus communis MA5.
10 500 gm.1 mg 25 ml 100 ug1.00 flask 100ul200 ug100ug Lyophilized
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#38647583 2022/08/29
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Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display.
Bacterial cis-epoxysuccinic acid hydrolases (CESHs) are intracellular enzymes used in the industrial production of enantiomeric tartaric acids. The enzymes are mainly used as whole-cell catalysts because of the low stability of purified CESHs. However, the low cell permeability is the major drawback of the whole-cell catalyst. To overcome this problem, we developed whole-cell catalysts using various surface display systems for CESH[L] which produces L(+)-tartaric acid. Considering that the display efficiency depends on both the carrier and the passenger, we screened five different anchoring motifs in Escherichia coli. Display efficiencies are significantly different among these five systems and the InaPbN-CESH[L] system has the highest whole-cell enzymatic activity. Conditions for InaPbN-CESH[L] production were optimized and a maturation step was discovered which can increase the whole-cell activity several times. After optimization, the total activity of the InaPbN-CESH[L] surface display system is higher than the total lysate activity of an intracellular CESH[L] overexpression system, indicating a very high CESH[L] display level. Furthermore, the whole-cell InaPbN-CESH[L] biocatalyst exhibited good storage stability at 4 °C and considerable reusability. Thereby, an efficient whole-cell CESH[L] biocatalyst was developed in this study, which solves the cell permeability problem and provides a valuable system for industrial L(+)-tartaric acid production.Rui Zhou, Sheng Dong, Yingang Feng, Qiu Cui, Jinsong Xuan
1948 related Products with: Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display.
2 Pieces/Box2 Pieces/Box 5 G96T2 Pieces/Box1 ml2 Pieces/Box
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#38647569 2022/07/18
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Chemoenzymatic conversion of glycerol to lactic acid and glycolic acid.
Catalytic valorization of raw glycerol derived from biodiesel into high-value chemicals has attracted great attention. Here, we report chemoenzymatic cascade reactions that convert glycerol to lactic acid and glycolic acid. In the enzymatic step, a coenzyme recycling system was developed to convert glycerol into 1,3-dihydroxyacetone (DHA) with a yield of 92.3% in potassium phosphate buffer (300 mM, pH 7.1) containing 100 mM glycerol, 2 mM NAD, 242 U/mL glycerol dehydrogenase-GldA and NADH oxidase-SpNox at 30 °C. Subsequently, NaOH or NaClO catalyzes the formation of lactic acid and glycolic acid from DHA. The high yield of lactic acid (72.3%) and glycolic acid (78.2%) verify the benefit of the chemoenzymatic approaches.Yue Ma, Tianzhen Li, Zijian Tan, Long Ma, Haifeng Liu, Leilei Zhu
1218 related Products with: Chemoenzymatic conversion of glycerol to lactic acid and glycolic acid.
100Tests 100 G 25 G10 mg10 mg100 MG1 kg 100 G500 mg 1 G 1KG
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#38647045 2024/04/22
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Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a.
CRISPR-Cas systems have widely been adopted as genome editing tools, with two frequently employed Cas nucleases being SpyCas9 and LbCas12a. Although both nucleases use RNA guides to find and cleave target DNA sites, the two enzymes differ in terms of protospacer-adjacent motif (PAM) requirements, guide architecture and cleavage mechanism. In the last years, rational engineering led to the creation of PAM-relaxed variants SpRYCas9 and impLbCas12a to broaden the targetable DNA space. By employing their catalytically inactive variants (dCas9/dCas12a), we quantified how the protein-specific characteristics impact the target search process. To allow quantification, we fused these nucleases to the photoactivatable fluorescent protein PAmCherry2.1 and performed single-particle tracking in cells of Escherichia coli. From our tracking analysis, we derived kinetic parameters for each nuclease with a non-targeting RNA guide, strongly suggesting that interrogation of DNA by LbdCas12a variants proceeds faster than that of SpydCas9. In the presence of a targeting RNA guide, both simulations and imaging of cells confirmed that LbdCas12a variants are faster and more efficient in finding a specific target site. Our work demonstrates the trade-off of relaxing PAM requirements in SpydCas9 and LbdCas12a using a powerful framework, which can be applied to other nucleases to quantify their DNA target search.Lorenzo Olivi, Cleo Bagchus, Victor Pool, Ezra Bekkering, Konstantin Speckner, Hidde Offerhaus, Wen Y Wu, Martin Depken, Koen J A Martens, Raymond H J Staals, Johannes Hohlbein