Gentaur Pub

#38648296   2024/04/22 To Up

Polyphenol-Assisted Biomineralization of Metal-Organic Framework Nanoparticles for Precision Delivery of Therapeutic Proteins to Cancer Cells.

The delivery of proteins into the cytosol holds great promise for cell signaling manipulation and the development of precision medicine. However, this potency is challenged by achieving targeted and controlled delivery, specifically within diseased cells. In this study, we introduce a versatile and effective method for the precision delivery of therapeutic proteins to cancer cells by designing polyphenol-assisted biomineralization of zeolite imidazole framework-8 (ZIF-8). We demonstrate that by leveraging the strong noncovalent binding affinity of epigallocatechin gallate (EGCG) with both proteins and ZIF-8, our approach significantly enhances the biomineralization of ZIF-8, which in turn improves the efficiency of protein encapsulation and intracellular delivery. Moreover, the incorporation of EGCG within ZIF-8 enables controlled degradation of the nanoparticles and the selective release of the encapsulated proteins in cancer cells. This selective release is triggered by the oxidation of EGCG in response to the high levels of reactive oxygen species (ROS) found within cancer cells that destabilize the EGCG/ZIF-8 nanoparticles. We have further demonstrated the ability of EGCG/ZIF-8 to deliver a wide range of proteins into cancer cells, including bacterial virulence protein, to rewire cell signaling and prohibit tumor cell growth in a mouse xenograft model. Our strategy and findings underscore the potential of designing the EGCG/ZIF-8 interface for specific and controlled protein delivery for targeted cancer therapy.
Tianli Luo, Qizhen Zheng, Ji Liu, Rui Yao, Ming Wang

1743 related Products with: Polyphenol-Assisted Biomineralization of Metal-Organic Framework Nanoparticles for Precision Delivery of Therapeutic Proteins to Cancer Cells.

501mg101mg1 mg1001010021.00 flask

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#38648198   2024/04/22 To Up

The impact of phage and phage resistance on microbial community dynamics.

Where there are bacteria, there will be bacteriophages. These viruses are known to be important players in shaping the wider microbial community in which they are embedded, with potential implications for human health. On the other hand, bacteria possess a range of distinct immune mechanisms that provide protection against bacteriophages, including the mutation or complete loss of the phage receptor, and CRISPR-Cas adaptive immunity. While our previous work showed how a microbial community may impact phage resistance evolution, little is known about the inverse, namely how interactions between phages and these different phage resistance mechanisms affect the wider microbial community in which they are embedded. Here, we conducted a 10-day, fully factorial evolution experiment to examine how phage impact the structure and dynamics of an artificial four-species bacterial community that includes either Pseudomonas aeruginosa wild-type or an isogenic mutant unable to evolve phage resistance through CRISPR-Cas. Additionally, we used mathematical modelling to explore the ecological interactions underlying full community behaviour, as well as to identify general principles governing the impacts of phage on community dynamics. Our results show that the microbial community structure is drastically altered by the addition of phage, with Acinetobacter baumannii becoming the dominant species and P. aeruginosa being driven nearly extinct, whereas P. aeruginosa outcompetes the other species in the absence of phage. Moreover, we find that a P. aeruginosa strain with the ability to evolve CRISPR-based resistance generally does better when in the presence of A. baumannii, but that this benefit is largely lost over time as phage is driven extinct. Finally, we show that pairwise data alone is insufficient when modelling our microbial community, both with and without phage, highlighting the importance of higher order interactions in governing multispecies dynamics in complex communities. Combined, our data clearly illustrate how phage targeting a dominant species allows for the competitive release of the strongest competitor while also contributing to community diversity maintenance and potentially preventing the reinvasion of the target species, and underline the importance of mapping community composition before therapeutically applying phage.
Ellinor O Alseth, Rafael Custodio, Sarah A Sundius, Rachel A Kuske, Sam P Brown, Edze R Westra

2699 related Products with: The impact of phage and phage resistance on microbial community dynamics.

0.25 mL100ug100ug 6 ml 100 mg25 mg10 25 MG

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#38647965   2023/09/15 To Up

Thermophilic Moorella thermoacetica as a platform microorganism for C1 gas utilization: physiology, engineering, and applications.

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Dechen Jia, Wangshuying Deng, Peng Hu, Weihong Jiang, Yang Gu

1145 related Products with: Thermophilic Moorella thermoacetica as a platform microorganism for C1 gas utilization: physiology, engineering, and applications.

10 mg250 ml5 g0.2 mg10 mg0.1 mg25 mg10 mg1 mg1 LITRE96 Tests0.5 mg

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#38647893   2022/07/30 To Up

Production of free fatty acids from various carbon sources by Ogataea polymorpha.

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Yunxia Li, XiaoXin Zhai, Wei Yu, Dao Feng, Aamer Ali Shah, Jiaoqi Gao, Yongjin J Zhou

2946 related Products with: Production of free fatty acids from various carbon sources by Ogataea polymorpha.

1,000 tests100tests1001KG500gm1kg100gm500g50gm

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#38647886   2023/06/28 To Up

Construction of Cupriavidus necator displayed with superoxide dismutases for enhanced growth in bioelectrochemical systems.

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Ke Chen, Chunling Ma, Xiaolei Cheng, Yuhua Wang, Kun Guo, Ranran Wu, Zhiguang Zhu

2369 related Products with: Construction of Cupriavidus necator displayed with superoxide dismutases for enhanced growth in bioelectrochemical systems.

20ug500 MG2 Pieces/Box100.00 ug100.00 ug0.1ml (1mg/ml)10ug10ug 100 G100.00 ug250 mg

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#38647878   2023/03/02 To Up

Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction.

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Shiyu Liang, Yue Zhang, Liting Lyu, Shuang Wang, Zongbao K Zhao

2050 related Products with: Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction.

1 G 1 G100 mg300 units 5 G10 mg

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#38647822   2022/05/26 To Up

Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.

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Peng Cai, Yunxia Li, Xiaoxin Zhai, Lun Yao, Xiaojun Ma, Lingyun Jia, Yongjin J Zhou

1021 related Products with: Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.

1 mg100 µg1 mL100ug100ug6 ml100ug Lyophilized500 1mg2 mg100.00 ug0.5 mg

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#38647771   2022/10/22 To Up

Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.

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Yunfei Zhu, Gaofan Zheng, Xiujuan Xin, Junying Ma, Jianhua Ju, Faliang An

1491 related Products with: Combinatorial strategies for production improvement of anti-tuberculosis antibiotics ilamycins E/E from deep sea-derived Streptomyces atratus SCSIO ZH16 ΔilaR.

1 mg100μg0.1ml (1mg/ml)500 500 500 μg100ug100ug Lyophilized100ug1 mL100ug Lyophilized100μg

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#38647747   2022/05/18 To Up

A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis.

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Jing Ye, Yunjie Li, Yuqing Bai, Ting Zhang, Wei Jiang, Ting Shi, Zijian Wu, Yi-Heng P Job Zhang

2452 related Products with: A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis.

50 25mg400Tests

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