Search results for: (Trifluoromethoxy)benzene CAS: [456-55-3]
#32957048 2020/09/15 To Up
Alleviating product inhibition of Trichoderma reesei cellulase complex with a product-activated mushroom endoglucanase.Product inhibition of cellulase is a challenging issue in industrial processes. Here, we introduced a product-activated mushroom cellulase, PaCel3A from Polyporus arcularius, into Trichoderma reesei. The filter paper activity, carboxymethyl cellulase activity, and saccharification efficiency (substrate: pretreated rice straw, PRS) of transformants increased significantly with this enzyme (by 18.4-26.8%, 13.8-22.8%, and 17.0%, respectively). A mutant of PaCel3A, PaCel3AM, obtained based on B-factor analysis, saturated mutagenesis, and residual activity assay, showed improved thermostability. The PRS saccharification efficiency using the cellulase complex from T. reesei transformants overexpressing pacel3am increased by 56.4%-63.0%. In addition, the T. reesei cellulase complex obtained by adding the purified recombinant PaCel3AM from T. reesei (rCel3aM-tr) to hydrolyze PRS resulted in increased reducing sugar yields at all sampling points, outperforming the cellulase complexes without rCel3aM-tr. These results suggest that introducing product-activated cellulase genes is a simple and feasible method to alleviate the product inhibition of cellulase.
Gen Zou, Dapeng Bao, Ying Wang, Sichi Zhou, Meili Xiao, Zhanshan Yang, Yinmei Wang, Zhihua Zhou
2340 related Products with: Alleviating product inhibition of Trichoderma reesei cellulase complex with a product-activated mushroom endoglucanase.1 ml50g100 ul Product tipe: Anti0.2 mg25 µgcase50 ug Product tipe: Antib500g 100 TESTS250 ml1 L.50 ug Product tipe: Antib
#32955851 2020/09/21 To Up
Intracellular binding/unbinding kinetics of approved drugs to carbonic anhydrase II observed by in-cell NMR.Candidate drugs rationally designed in vitro often fail due to low efficacy in vivo caused by low tissue availability or because of unwanted side effects. To overcome the limitations of in vitro rational drug design, the binding of candidate drugs to their target needs to be evaluated in the cellular context. Here, we applied in-cell NMR to investigate the binding of a set of approved drugs to the isoform II of carbonic anhydrase (CA) in living human cells. Some compounds were originally developed towards other targets, and were later found to inhibit CAs. We observed strikingly different dose- and time-dependent binding, wherein some drugs exhibited a more complex behavior than others. Specifically, some compounds were shown to gradually unbind from intracellular CA II, even in the presence of free compound in the external medium, therefore preventing the quantitative formation of a stable protein-ligand complex. Such observations could be correlated to the known off-target binding activity of these compounds, suggesting that this approach could provide information on the pharmacokinetic profiles of lead candidates at the early stages of multi-target drug design.
Enrico Luchinat, Letizia Barbieri, Matteo Cremonini, Alessio Nocentini, Claudiu T Supuran, Lucia Banci
1756 related Products with: Intracellular binding/unbinding kinetics of approved drugs to carbonic anhydrase II observed by in-cell NMR.1 kit1 kit1.00 flask25 Tests100 tests1 kit1 kit25 Tests1 kit96T50 mg
#32955088 2020/09/21 To Up
Rezafungin versus Caspofungin in a Phase 2, Randomized, Double-Blind Study for the Treatment of Candidemia and Invasive Candidiasis- The STRIVE Trial.Rezafungin (RZF) is a novel echinocandin exhibiting distinctive pharmacokinetics/pharmacodynamics. STRIVE was a phase 2, double-blind, randomized trial designed to compare the safety and efficacy of RZF once weekly (QWk) to caspofungin (CAS) once daily for treatment of candidemia and/or invasive candidiasis (IC).
George R Thompson, Alex Soriano, Athanasios Skoutelis, Jose A Vazquez, Patrick M Honore, Juan P Horcajada, Herbert Spapen, Matteo Bassetti, Luis Ostrosky-Zeichner, Anita F Das, Rolando M Viani, Taylor Sandison, Peter G Pappas
2791 related Products with: Rezafungin versus Caspofungin in a Phase 2, Randomized, Double-Blind Study for the Treatment of Candidemia and Invasive Candidiasis- The STRIVE Trial.1
#32954763 2020/09/14 To Up
Ma-Nuo-Xi decoction has an immunostimulating effect in cyclophosphamide-immunosuppressed mice.Ma-Nuo-Xi decoction (MNXD), as well as its hundreds of derivative preparations, has been used in Tibetan medicine since the 14th century. MNXD is in accordance with the theory of treatment determination based on syndrome differentiation. This study aimed to compare the effect of the auxiliary MNXD prescription (MNXD-AD) with that of the basic MNXD prescription (MNXD-BD) on the immunostimulating activity of MNXD.
Duo Jie, Zhongshu Shan, Zhiliang Ma, Ming Zhang, Tingting Gao, Hao Wu, Bin Shan, Lixin Wei, Hongtao Bi
1771 related Products with: Ma-Nuo-Xi decoction has an immunostimulating effect in cyclophosphamide-immunosuppressed mice.1 Set1 Set1 Set100 μg1 Set1 Set100 μg4 Membranes/Box100 μg1 Set0.2 mg
#32954652 2020/09/20 To Up
A Hybrid Organo-Nanotheranostic Platform of Superlative Biocompatibility for Near-Infrared-Triggered Fluorescence Imaging and Synergistically Enhanced Ablation of Tumors.The quest for an all-organic nanosystem with negligible cytotoxicity and remarkable in vivo tumor theranostic capability is inescapably unending. Hitherto, the landscape of available photothermal agents is dominated by metal-based nanoparticles (NPs) with attendant in vivo negatives. Here, an all-organic-composed theranostic nanosystem with outstanding biocompatibility for fluorescence image-guided tumor photothermal therapy, and as a potential alternative to metal-based photothermal agents is developed. This is rationally achieved by compartmentalizing indocyanine green (ICG) in glycol chitosan (GC)-polypyrrole (PP) nanocarrier to form hybrid [email protected] NPs (≈65 nm). The compartmentalization strategy, alongside the high photothermal conversion ability of PP jointly enhances the low photostability of free ICG. Advantageously, [email protected] is endowed with an impeccable in vivo performance by the well-known biocompatibility track records of its individual tri organo-components (GC, PP, and ICG). As a proof of concept, [email protected] NPs enables a sufficiently prolonged tumor diagnosis by fluorescence imaging up to 20 h post-injection. Furthermore, owing to the complementary heating performances of PP and ICG, [email protected] NPs-treated mice by one-time near-infrared irradiation exhibit total tumor regression within 14 days post-treatment. Therefore, leveraging the underlying benefits of this study will help to guide the development of new all-organic biocompatible systems in synergism, for safer tumor theranostics.
Ozioma Udochukwu Akakuru, Chuang Liu, M Zubair Iqbal, Gohar Ijaz Dar, Gao Yang, Kun Qian, Elvis Ikechukwu Nosike, Jie Xing, Zhoujing Zhang, Yanying Li, Juan Li, Aiguo Wu
2291 related Products with: A Hybrid Organo-Nanotheranostic Platform of Superlative Biocompatibility for Near-Infrared-Triggered Fluorescence Imaging and Synergistically Enhanced Ablation of Tumors.1 kit1 kit1 kit1 kit1 kit 5 G250 ml1 kit100 mg 1 G100 plates25 g
#32954573 2020/09/21 To Up
Liquid Metal Initiator of Ring-Opening Polymerization: Self-Capsulation into Thermal/Photomoldable Powder for Multifunctional Composites.Liquid metal nanodroplets not only share similar metallic properties and nanoscale effect with solid metal nanoparticles, but also possess the additional uniqueness in nonvolatile fluidity and ambient sintering ability into continuous conductors. In most cases, liquid metal nanodroplets are encapsulated into ultrathin and fragile shells of oxides and amphiphile monolayers, and may be hindered from incorporating homogeneously into various composites through conventional processing methods. In this study, ring-opening polymerization is found to be initiated by sonicating the liquid metal EGaIn in fluidic lactones. By this in situ polymerization, EGaIn nanodroplets are encapsulated into polylactone shells with tunable thickness, which can further be dried into a solid powder. Besides high chemical stability and dispersibility in organic solvents, the powder of the EGaIn capsules combines the exceptional properties of the EGaIn droplets (e.g., photothermal effect) and the polylactone shells (e.g., biocompatibility, biodegradability, and compatibility with different polymer matrixes), being capable of being introduced into thermoplastic composites through liquid casting and thermal- or photomolding for the notch-insensitive tearing property, sintering-induced electric conductivity, and photothermal effect. Thus, the EGaIn initiator of ring-opening polymerization may start a pathway to produce stable andthermal/photomoldable powders of EGaIn capsules and their multifunctionalcomposites, applicable in biomedicines, soft electronics, and smart robots.
Xiankai Li, Mingjie Li, Qinghui Shou, Li Zhou, Anle Ge, Danfeng Pei, Chaoxu Li
2658 related Products with: Liquid Metal Initiator of Ring-Opening Polymerization: Self-Capsulation into Thermal/Photomoldable Powder for Multifunctional Composites.25 mg1 g250 g100μg100 mg100μg10 mg100μg50 ug25 μg 5 lt20
#32954022 2020/09/02 To Up
CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants.CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) has been extensively exploited as a genetic tool for genome editing. The RNA guided Cas nucleases generate DNA double-strand break (DSB), triggering cellular repair systems mainly Non-homologous end-joining (NHEJ, imprecise repair) or Homology-directed repair (HDR, precise repair). However, DSB typically leads to unexpected DNA changes and lethality in some organisms. The establishment of bacteria and plants into major bio-production platforms require efficient and precise editing tools. Hence, in this review, we focus on the non-DSB and template-free genome editing, i.e., base editing (BE) and prime editing (PE) in bacteria and plants. We first highlight the development of base and prime editors and summarize their studies in bacteria and plants. We then discuss current and future applications of BE/PE in synthetic biology, crop improvement, evolutionary engineering, and metabolic engineering. Lastly, we critically consider the challenges and prospects of BE/PE in PAM specificity, editing efficiency, off-targeting, sequence specification, and editing window.
Abdullah, Zhengzheng Jiang, Xulin Hong, Shun Zhang, Ruilian Yao, Yi Xiao
2201 related Products with: CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants.5 G2.5 mg10 mg100ug1,000 tests1 mg2.5 mg50 ug 1000 tests1000 TESTS/0.65ml100ug
#32953742 // To Up
Effect of combined chronic predictable and unpredictable stress on depression-like symptoms in mice.Mental stress mainly induces depression, and predictable stress, as well as a constant bombardment of chronic unpredictable micro-stressors, always coexist in daily life. However, the combined effect of predictable and unpredictable stress on depression is still not fully understood.
Yajun Qiao, Jiubo Zhao, Cen Li, Ming Zhang, Lixin Wei, Xiaoyuan Zhang, Olga Kurskaya, Hongtao Bi, Tingting Gao
1427 related Products with: Effect of combined chronic predictable and unpredictable stress on depression-like symptoms in mice.100 UG 50 UG100ug100ug100ug Lyophilized1 Set1 Set
#32953248 2020/09/01 To Up
Piloting a Deep Learning Model for Predicting Nuclear BAP1 Immunohistochemical Expression of Uveal Melanoma from Hematoxylin-and-Eosin Sections.Uveal melanoma (UM) is the most common primary intraocular malignancy in adults. Monosomy 3 and mutation are strong prognostic factors predicting metastatic risk in UM. Nuclear BAP1 (nBAP1) expression is a close immunohistochemical surrogate for both genetic alterations. Not all laboratories perform routine BAP1 immunohistochemistry or genetic testing, and rely mainly on clinical information and anatomic/morphologic analyses for UM prognostication. The purpose of our study was to pilot deep learning (DL) techniques to predict nBAP1 expression on whole slide images (WSIs) of hematoxylin and eosin (H&E) stained UM sections.
Hongrun Zhang, Helen Kalirai, Amelia Acha-Sagredo, Xiaoyun Yang, Yalin Zheng, Sarah E Coupland
2533 related Products with: Piloting a Deep Learning Model for Predicting Nuclear BAP1 Immunohistochemical Expression of Uveal Melanoma from Hematoxylin-and-Eosin Sections.0.1ml (1mg/ml)0.2 mg100ug Lyophilized 125 ml 100ul100ug100 mg
#32953198 2020/09/15 To Up
Phage capsid nanoparticles as multivalent inhibitors of viral infections.
Manzar Abbas, Muhammad Ovais, Chunying Chen
1920 related Products with: Phage capsid nanoparticles as multivalent inhibitors of viral infections.400Tests1 mg100 plates100400 assays200 assays1000 assays50 assays100 ul100ug Lyophilized
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