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#33662400   2021/03/01 To Up

SSSPTA is essential for serine palmitoyltransferase function during development and hematopoiesis.

Serine palmitoyltransferase complex (SPT) mediates the first and rate-limiting step in the de novo sphingolipid biosynthetic pathway. The larger subunits SPTLC1 and SPTLC2/SPTLC3 together form the catalytic core while a smaller third subunit either SSSPTA or SSSPTB has been shown to increase the catalytic efficiency and provide substrate specificity for the fatty acyl-CoA substrates. The in vivo biological significance of these smaller subunits in mammals is still unknown. Here, using two null mutants, a conditional null for ssSPTa and a null mutant for ssSPTb we show that SSSPTA is essential for embryogenesis and mediates much of the known functions of the SPT complex in mammalian hematopoiesis. The ssSPTa null mutants are embryonic lethal at E6.5 much like the Sptlc1 and Sptlc2 null alleles. Mx1-Cre induced deletion of ssSPTa leads to lethality and myelopoietic defect. Chimeric and competitive bone marrow transplantation experiments show that the defect in myelopoiesis is accompanied by an expansion of the LinSca1c-Kit (LSK) stem and progenitor compartment. Progenitor cells that fail to differentiate along the myeloid lineage display evidence of endoplasmic reticulum stress. On the other hand, ssSPTb null mice are homozygous viable and analyses of the bone marrow cells show no significant difference in the proliferation and differentiation of the adult hematopoietic compartment. SPTLC1 is an obligatory subunit for the SPT function and since Sptlc1 and ssSPTa mice display similar defects during development and hematopoiesis, we conclude that an SPT complex that includes SSSPTA mediates much of its developmental and hematopoietic functions in a mammalian model.
Velayoudame Parthibane, Jing Lin, Diwash Acharya, Thiruvaimozhi Abimannan, Sargur Madabushi Srideshikan, Kimberly Klarmann, Acong Yang, Ferri Soheilian, Kunio Nagashima, Stephen D Fox, Thorkell Andresson, Lino Tessarollo, Jonathan R Keller, Usha Acharya, Jairaj K Acharya

2863 related Products with: SSSPTA is essential for serine palmitoyltransferase function during development and hematopoiesis.

250ul0.1 ml0.1ml (1mg/ml)25 µg1 g0.1 mg50ul (1mg/ml)0.25 mg1 mg1 ml0.2 mg

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#33662257   2021/02/25 To Up

Maintaining the structural and functional homeostasis of the plant endoplasmic reticulum.

The endoplasmic reticulum (ER) is a ubiquitous organelle that is vital to the life of eukaryotic cells. It synthesizes essential lipids and proteins and initiates the glycosylation of intracellular and surface proteins. As such, the ER is necessary for cell growth and communication with the external environment. The ER is also a highly dynamic organelle, whose structure is continuously remodeled through an interaction with the cytoskeleton and the action of specialized ER shapers. Recent and significant advances in ER studies have brought to light conserved and unique features underlying the structure and function of this organelle in plant cells. In this review, exciting developments in the understanding of the mechanisms for plant ER structural and functional homeostasis, particularly those that underpin ER network architecture and ER degradation, are presented and discussed.
Federica Brandizzi

2839 related Products with: Maintaining the structural and functional homeostasis of the plant endoplasmic reticulum.

121 ml50 IU100.00 ul1500 Units 100ul 100 G

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#33662063   2021/03/03 To Up

Laser retrieval of cemented zirconia single unit implant restoration: A pilot study: Part I - Force values.

To measure the retrieval force required to remove 1.5-mm-thick CAD/CAM zirconia copings cemented on zirconia (Zr) and titanium (Ti) stock implant abutments after a single application of erbium-doped yttrium scandium gallium garnet (Er:YSGG) laser.
Karnik Shah, Edwin McGlumphy, Damian J Lee, Robert R Seghi, D Stanley Sharples, Do-Gyoon Kim

1762 related Products with: Laser retrieval of cemented zirconia single unit implant restoration: A pilot study: Part I - Force values.

25 Bags/Unit50ug100 ug50ug300 units100 units100ug Lyophilized100ug Lyophilized10 mg100ug Lyophilized100ug Lyophilized100ug

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#33661913   2021/03/04 To Up

Independent predictors of acute kidney injury in patients with acute coronary syndrome after percutaneous coronary intervention.

High residual platelet reactivity (RPR) in patients after percutaneous coronary intervention (PCI) receiving antiplatelet agents has been associated with a high risk of developing acute kidney injury (AKI).
Aisulu Zhunuspekova, Jamilya Mansurova, Lyudmila Karazhanova

1601 related Products with: Independent predictors of acute kidney injury in patients with acute coronary syndrome after percutaneous coronary intervention.

96T 100ul1.00 flask

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#33661876   // To Up

Manejo de la insuficiencia cardiaca con estimulación hisiana en bloqueo de rama derecha: reporte de caso.

La terapia de resincronización cardiaca mediante estimulación hisiana ha demostrado ser efectiva en pacientes con bloqueo de rama izquierda del haz de His e insuficiencia cardiaca. Paciente masculino, con 47 años de edad, con insuficiencia cardiaca, fracción de expulsión del 17% y miocardio dilatada idiopática, electrocardiograma en ritmo sinusal, bloqueo auriculoventricular de 1. grado, intervalo PR 400 ms, bloqueo completo de rama derecha del haz de His, bloqueo del fascículo anterior de la rama izquierda del haz de His, duración del QRS 200 ms. Se decidió realizar estimulación selectiva del haz de His. La resincronización cardiaca biventricular convencional en pacientes con presencia de bloqueo completo de la rama derecha del haz de His no está indicada debido a la pobre respuesta al tratamiento. La estimulación hisiana permite reclutar la rama bloqueada y reestablecer la conducción a través de ella, de tal forma que, en ausencia de necrosis, se logre sincronía biventricular. En el caso presentado el reclutamiento de la rama derecha mediante estimulación hisiana se reflejó en el restablecimiento de la sincronía biventricular, medida por rastreo de marcas (speckle tracking) e incremento significativo de la fracción de expulsión del ventrículo izquierdo del 17 al 36.6%, con un incremento absoluto del 19.6%.
Jorge Gómez-Flores, Jovana Pérez-Báez, Guillermo Muñoz, Gabriela Bustillos-García, Alan García, Álvaro E Reyes-Quintero, Rosa M Ávila-Ocampo, Antonio Arias-Godínez, Manlio Márquez, Luis Colin-Lizalde, Moisés Levinstein-Jacinto, José L Morales-Velázquez, Santiago Nava

1770 related Products with: Manejo de la insuficiencia cardiaca con estimulación hisiana en bloqueo de rama derecha: reporte de caso.

100Tests1 x 961 kit50 assays100tests100Tests100tests100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#33661766   2021/03/04 To Up

Cell death-associated lipid droplet protein CIDE-A is a non-canonical marker of endoplasmic reticulum stress.

Secretory protein misfolding has been linked to ER stress and cell death. We expressed a TGrdw transgene encoding TG-G(2298)R, a misfolded mutant thyroglobulin reported to be linked to thyroid cell death. When the TGrdw transgene was expressed at low-level in thyrocytes of TGcog/cog mice that experience severe ER stress, we observed increased thyrocyte cell death and increased expression of CIDE-A (Cell death-inducing DFFA-like effector-A, a protein of lipid droplets) in whole thyroid gland. Here we demonstrate that acute ER stress in cultured PCCL3 thyrocytes increases Cidea mRNA levels, maintained at least in part by increased mRNA stability, while being negatively regulated by ATF6 - with similar observations other cell types. CIDE-A protein is sensitive to proteasomal degradation yet is stabilized by ER stress, and elevated expression levels accompany increased cell death. Unlike acute ER stress, PCCL3 cells adapted and surviving chronic ER stress maintain a disproportionately lower relative mRNA level of Cidea compared to that of other, classical ER stress markers, as well as a blunted Cidea mRNA response to a new, unrelated acute ER stress challenge. We suggest that CIDE-A is a novel marker linked to a non-canonical ER stress-response program, with implications for cell death and survival.
Yoshiaki Morishita, Aaron P Kellogg, Dennis Larkin, Wei Chen, Suryakiran Vadrevu, Leslie S Satin, Ming Liu, Peter Arvan

2636 related Products with: Cell death-associated lipid droplet protein CIDE-A is a non-canonical marker of endoplasmic reticulum stress.

100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized 100ul100ug Lyophilized100ug Lyophilized100ug Lyophilized

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#33661518   2021/03/04 To Up

Chemical chaperones targeted to the endoplasmic reticulum (ER) and lysosome prevented neurodegeneration in a C9orf72 repeat expansion drosophila amyotrophic lateral sclerosis (ALS) model.

ALS is an incurable neuromuscular degenerative disorder. A familiar form of the disease (fALS) is related to point mutations. The most common one is an expansion of a noncoding GGGGCC hexanucleotide repeat of the C9orf72 gene on chromosome 9p21. An abnormal translation of the C9orf72 gene generates dipeptide repeat proteins that aggregate in the brain. One of the classical approaches for developing treatment against protein aggregation-related diseases is to use chemical chaperones (CSs). In this work, we describe the development of novel 4-phenylbutyric acid (4-PBA) lysosome/ER-targeted derivatives. We assumed that 4-PBA targeting to specific organelles, where protein degradation takes place, might reduce the 4-PBA effective concentration.
Salome Azoulay-Ginsburg, Michela Di Salvio, Michal Weitman, Michal Afri, Sara Ribeiro, Simon Ebbinghaus, Gianluca Cestra, Arie Gruzman

2718 related Products with: Chemical chaperones targeted to the endoplasmic reticulum (ER) and lysosome prevented neurodegeneration in a C9orf72 repeat expansion drosophila amyotrophic lateral sclerosis (ALS) model.

11 mg100 μg1 kit1 kit

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#33660830   // To Up

Androgen receptor expression and outcome of neoadjuvant chemotherapy in triple-negative breast cancer.

Triple-negative breast cancers (TNBC) include a heterogeneous group of diseases, characterized by the lack of estrogen receptor (ER), progesterone receptor (PgR), and human epidermal growth factor receptor 2 (HER2) expression. TNBC that shows an overexpression of the androgen receptor (AR) defines the phenotype known as "luminal androgen receptor" (LAR), while the absence of the AR defines a "quadruple negative breast cancer" (QNBC). Several reports have associated AR positivity with a lower response to neoadjuvant chemotherapy (NAC), while divergent data have been reported about the impact of AR positivity on survival. The aim of this study was to retrospectively review our series of patients with TNBC tested for AR and submitted to NAC and compare pathologic complete response (pCR) rates in patients with a LAR phenotype or with QNBC.
A Di Leone, S M Fragomeni, L Scardina, L Ionta, A Mulè, S Magno, D Terribile, R Masetti, G Franceschini

1634 related Products with: Androgen receptor expression and outcome of neoadjuvant chemotherapy in triple-negative breast cancer.

100ug50 ug 100ug

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#33660790   // To Up

Protective effect of estrogen receptors (ERα/β) against the intervertebral disc degeneration involves activating CCN5 via the promoter.

Due to the decrease of estrogen and estrogen receptor (ER) in postmenopausal women, they have a higher risk of intervertebral disc degeneration (IDD) than men. This study aims to explore how ERα and ERb interact with CCN5 and protect IDD.
M-X Song, X-X Ma, C Wang, Y Wang, C Sun, D-R Xu, K Zhu, G-H Li, H Zhao, C Zhang

1988 related Products with: Protective effect of estrogen receptors (ERα/β) against the intervertebral disc degeneration involves activating CCN5 via the promoter.

500 Units500 Units100 2000 IU500IU 100 G1100.00 ul

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