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Search results for: Gag Polymerase (Retro Virus)

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#12634845   // To Up

The soybean retroelement SIRE1 uses stop codon suppression to express its envelope-like protein.

The soybean SIRE1 family of Ty1/copia retrotransposons encodes an envelope-like gene (env-like). We analysed the DNA sequences of nine SIRE1 insertions and observed that the gag/pol and env-like genes are in the same reading frame and separated by a single UAG stop codon. The six nucleotides immediately downstream of the stop codon conform to a degenerate nucleotide motif, CARYYA, which is sufficient to facilitate stop codon suppression in tobacco mosaic virus. In vivo stop codon suppression assays indicate that SIRE1 sequences confer leakiness to the UAG stop codon at an efficiency of 5%. These data suggest that SIRE1 retro-elements use translational suppression to express their envelope-like protein; this is in contrast with all characterized retroviruses, which express the envelope protein from a spliced genomic messenger RNA.
Ericka R Havecker, Daniel F Voytas

1740 related Products with: The soybean retroelement SIRE1 uses stop codon suppression to express its envelope-like protein.

480/kit100 1000 100 U1mg100 24 reactions 100 5 reactions

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#12472123   // To Up

Endogenous jaagsiekte sheep retrovirus RNA is expressed by different cell types in ovine foetus and placenta.

The endogenous retroviruses are inherited elements transmitted trough the germline of most animal species and their biological role is still controversial. Ovine Pulmonary Carcinoma (OPC) represents a good model for studying the interactions of endogenous retroviruses with their exogenous counterparts. The type D exogenous retrovirus known as Jaagsiekte Sheep Retro-Virus (JSRV) is necessary and sufficient to cause OPC in domestic and wild sheep, but both affected and unaffected animals host in their genome 15 to 20 copies of related endogenous retroviruses named endogenous JSRV (enJSRV). In this study we evaluated the expression of enJSRV gag sequences in ovine foetal and placental tissues. RNA in situ hybridisation was performed on tissue sections of thymi, lymph nodes and lungs from ovine foetuses and related placentas, taken at a late stage of development. Reverse transcriptase-in situ polymerase chain reactions were also carried out on placental samples to better define the involved cells. In foetal tissues, specific signals were observed in the thymus medulla, lymph nodes and, at a lesser extent, in foetal bronchiolar cells. In the placental tissues, positive areas were detected in various cell types in the sincythium-and cyto-trophoblast. These data demonstrate that en JSRV RNA is largely expressed in a broad spectrum of cells including tissues which are critical for the development of the immune system.
E Sanna, M P Sanna, C Loddo, L Sanna, M Mura, T Cadelano, A Leoni

2012 related Products with: Endogenous jaagsiekte sheep retrovirus RNA is expressed by different cell types in ovine foetus and placenta.

100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized1 mg100ug Lyophilized100ug Lyophilized100ug Lyophilized100ug Lyophilized10 ug100ug Lyophilized100ug Lyophilized

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#11591216   2001/08/10 To Up

Evolution and phylogeny of insect endogenous retroviruses.

The genome of invertebrates is rich in retroelements which are structurally reminiscent of the retroviruses of vertebrates. Those containing three open reading frames (ORFs), including an env-like gene, may well be considered as endogenous retroviruses. Further support to this similarity has been provided by the ability of the env-like gene of DmeGypV (the Gypsy endogenous retrovirus of Drosophila melanogaster) to promote infection of Drosophila cells by a pseudotyped vertebrate retrovirus vector.
C Terzian, A Pélisson, A Bucheton

1561 related Products with: Evolution and phylogeny of insect endogenous retroviruses.

0.1ml (1mg/ml)1,000 tests100ul1 mg500 ml1000 tests100.00 ul100ug100 mg1x10e7 cells200

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