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#33647250   2021/02/26 To Up

Peripheral blood cultured mast cells: Phenotypic and functional outcomes of different culture protocols.

Mast cells (MCs) play a pivotal role in innate and adaptive immune responses. However, MCs are also involved in different pathologic conditions. Studies on the mechanisms that govern human MC functions are impeded by their limited and difficult recovery. Therefore, several research groups have developed protocols to culture human MCs from progenitor cells. These protocols vary with respect to culture duration and used maturation cytokines. How MCs obtained by different protocols differ in phenotype and functionality is currently unknown.
Jessy Elst, Vito Sabato, Marie-Line M van der Poorten, Margaretha Faber, Athina L Van Gasse, Leander P De Puysseleyr, Chris H Bridts, Christel Mertens, Michel Van Houdt, Marcus Maurer, Margo M Hagendorens, Didier G Ebo

2146 related Products with: Peripheral blood cultured mast cells: Phenotypic and functional outcomes of different culture protocols.

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#33639037   2021/02/27 To Up

Platelet-Activating Immune Complexes Identified in Critically Ill COVID-19 Patients Suspected of Heparin-Induced Thrombocytopenia.

Thrombocytopenia and thrombosis are prominent in coronavirus disease 2019 (COVID-19), particularly amongst critically ill patients; however, the mechanism is unclear. Such critically ill COVID-19 patients may be suspected of heparin-induced thrombocytopenia (HIT), given similar clinical features.
Ishac Nazy, Stefan D Jevtic, Jane C Moore, Angela Huynh, James W Smith, John G Kelton, Donald M Arnold

1172 related Products with: Platelet-Activating Immune Complexes Identified in Critically Ill COVID-19 Patients Suspected of Heparin-Induced Thrombocytopenia.

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#33600815   2021/02/15 To Up

Lipoxin A4 regulates microglial M1/M2 polarization after cerebral ischemia-reperfusion injury via the Notch signaling pathway.

Microglia are rapidly activated after acute ischemic stroke, and the polarization of microglial is associated with the prognosis of acute ischemic stroke. Lipoxin A4 (LXA4), an anti-inflammatory agent, has a protective effect against ischemic stroke. However, the role of LXA4 on the polarization of microglial after acute ischemic stroke remains undetermined. We hypothesized that LXA4 may exert the neuroprotective effect though regulating the polarization of microglial. In this study, clinical features of acute ischemic stroke were simulated using a rat model of model of middle cerebral artery occlusion (MCAO) in vivo and the BV2 microglia oxygen-glucose deprivation/reoxygenation model (OGD/R) in vitro. The protective effects of LXA4 on cerebral ischemia-reperfusion injury were determined using TTC staining, HE staining, and TUNEL staining. The expression of targeted genes was assayed using quantitative real-time PCR (qRT-PCR), immunofluorescence, and western blot to investigated the regulation of LXA4 on microglia polarization after acute ischemic stroke. We found that LXA4 exerted protective effects on focal cerebral ischemia-reperfusion injury and reduced the expression of the pro-inflammatory cytokines IL-1β and TNF-α. Furthermore, LXA4 inhibited the expression of Notch-1, Hes1, iNOS and CD32 all of which are associated with the differentiation into M1 microglia. By contrast, LXA4 upregulated the expression of Hes5, Arg-1 and CD206 all of which are associated with M2 phenotype in microglia. In addition, blocking the Notch signaling pathway with the inhibitor DAPT significantly mitigated the effect of LXA4 on microglia differentiation. These data suggest that LXA4 may regulate the polarization of microglia after cerebral ischemia-reperfusion injury through the Notch signaling pathway.
Qian-Qian Li, Dan-Hua Ding, Xin-Yu Wang, Yu-Ying Sun, Jun Wu

1999 related Products with: Lipoxin A4 regulates microglial M1/M2 polarization after cerebral ischemia-reperfusion injury via the Notch signaling pathway.

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#33593344   2021/02/16 To Up

Genetic variation in the immune system and malaria susceptibility in infants: a nested case-control study in Nanoro, Burkina Faso.

Genetic polymorphisms in the human immune system modulate susceptibility to malaria. However, there is a paucity of data on the contribution of immunogenetic variants to malaria susceptibility in infants, who present differential biological features related to the immaturity of their adaptive immune system, the protective effect of maternal antibodies and fetal haemoglobin. This study investigated the association between genetic variation in innate immune response genes and malaria susceptibility during the first year of life in 656 infants from a birth cohort survey performed in Nanoro, Burkina Faso.
Hamatandi Magloire Natama, Eduard Rovira-Vallbona, Meryam Krit, Pieter Guetens, Hermann Sorgho, M Athanase Somé, Maminata Traoré-Coulibaly, Innocent Valéa, Petra F Mens, Henk D F H Schallig, Dirk Berkvens, Luc Kestens, Halidou Tinto, Anna Rosanas-Urgell

2408 related Products with: Genetic variation in the immune system and malaria susceptibility in infants: a nested case-control study in Nanoro, Burkina Faso.



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#33552684   2021/01/25 To Up

Cyclophosphamide alters the tumor cell secretome to potentiate the anti-myeloma activity of daratumumab through augmentation of macrophage-mediated antibody dependent cellular phagocytosis.

Multiple Myeloma (MM) is a malignant disorder of plasma cells which, despite significant advances in treatment, remains incurable. Daratumumab, the first CD38 directed monoclonal antibody, has shown promising activity alone and in combination with other agents for MM treatment. Daratumumab is thought to have pleiotropic mechanisms of activity including natural killer (NK) cell-mediated antibody-dependent cellular cytotoxicity (ADCC). With the knowledge that CD38-expressing NK cells are depleted by daratumumab, we sought to investigate a potential mechanism of enhancing macrophage-mediated antibody-dependent cellular phagocytosis (ADCP) by combining daratumumab with cyclophosphamide (CTX). Cyclophosphamide's immunomodulatory function was investigated by conditioning macrophages with tumor cell secretome collected from cyclophosphamide treated MM cell lines (CTX-TCS). Flow cytometry analysis revealed that CTX-TCS conditioning augmented the migratory capacity of macrophages and increased CD32 and CD64 Fcγ receptor expression on their cell surface. Daratumumab-specific tumor clearance was increased by conditioning macrophages with CTX-TCS in a dose-dependent manner. This effect was impeded by pre-incubating macrophages with Cytochalasin D (CytoD), an inhibitor of actin polymerization, indicating macrophage-mediated ADCP as the mechanism of clearance. CD64 expression on macrophages directly correlated with MM cell clearance and was essential to the observed synergy between cyclophosphamide and daratumumab, as tumor clearance was attenuated in the presence of a FcγRI/CD64 blocking agent. Cyclophosphamide independently enhances daratumumab-mediated killing of MM cells by altering the tumor microenvironment to promote macrophage recruitment, polarization to a pro-inflammatory phenotype, and directing ADCP. These findings support the addition of cyclophosphamide to existing or novel monoclonal antibody-containing MM regimens.
Serika D Naicker, Claire L Feerick, Kevin Lynch, Dawn Swan, Cian McEllistrim, Robert Henderson, Niamh A Leonard, Oliver Treacy, Alessandro Natoni, Athina Rigalou, Joana Cabral, Christopher Chiu, Kate Sasser, Thomas Ritter, Michael O'Dwyer, Aideen E Ryan

2204 related Products with: Cyclophosphamide alters the tumor cell secretome to potentiate the anti-myeloma activity of daratumumab through augmentation of macrophage-mediated antibody dependent cellular phagocytosis.

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#33493550   2021/01/22 To Up

Human peripheral basophils extended phenotype shows a high expression of CD244 immuno-regulatory receptor.

Basophils play a major physio-pathological role in hypersensitivity related diseases. Basophils express high affinity Immunoglobulin (Ig) E receptors (FcεRI), IgG and complement regulatory. Basophils also have immunoregulatory activity through interaction with T cells. The aim of this study was to look for the expression of markers reflecting the activation status of peripheral Basophil in healthy donors.
Anne-Emmanuelle Berger, Coralie Durrieu, Charles Dzviga, Jean-Luc Perrot, Claude Lambert

1408 related Products with: Human peripheral basophils extended phenotype shows a high expression of CD244 immuno-regulatory receptor.

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#33364576   2020/11/30 To Up

CD32CD4 memory T cells are enriched for total HIV-1 DNA in tissues from humanized mice.

CD32 has raised conflicting results as a putative marker of the HIV-1 reservoir. We measured CD32 expression in tissues from viremic and virally suppressed humanized mice treated relatively early or late after HIV-1 infection with combined antiretroviral therapy. CD32 was expressed in a small fraction of the memory CD4 T-cell subsets from different tissues in viremic and aviremic mice, regardless of treatment initiation time. CD32 memory CD4 T cells were enriched in cell-associated (CA) HIV-1 DNA but not in CA HIV-1 RNA as compared to the CD32CD4 fraction. Using multidimensional reduction analysis, several memory CD4CD32 T-cell clusters were identified expressing HLA-DR, TIGIT, or PD-1. Importantly, although tissue-resident CD32CD4 memory cells were enriched with translation-competent reservoirs, most of it was detected in memory CD32CD4 T cells. Our findings support that CD32 labels highly activated/exhausted memory CD4 T-cell subsets that contain only a small proportion of the translation-competent reservoir.
Philipp Adams, Virginie Fievez, Rafaëla Schober, Mathieu Amand, Gilles Iserentant, Sofie Rutsaert, Géraldine Dessilly, Guido Vanham, Fanny Hedin, Antonio Cosma, Michel Moutschen, Linos Vandekerckhove, Carole Seguin-Devaux

2600 related Products with: CD32CD4 memory T cells are enriched for total HIV-1 DNA in tissues from humanized mice.

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#33312564   2020/12/08 To Up

Hepatitis B virus exploits C-type lectin receptors to hijack cDC1s, cDC2s and pDCs.

C-type lectin receptors (CLRs) are key receptors used by DCs to orchestrate responses to pathogens. During infections, the glycan-lectin interactions shape the virus-host interplay and viruses can subvert the function of CLRs to escape antiviral immunity. Recognition of virus/viral components and uptake by CLRs together with subsequent signalling cascades are crucial in initiating and shaping antiviral immunity, and decisive in the outcome of infection. Yet, the interaction of hepatitis B virus (HBV) with CLRs remains largely unknown. As HBV hijacks DC subsets and viral antigens harbour glycan motifs, we hypothesised that HBV may subvert DCs through CLR binding.
Laurissa Ouaguia, Tania Dufeu-Duchesne, Vincent Leroy, Thomas Decaens, Jean-Baptiste Reiser, Eleonora Sosa Cuevas, David Durantel, Jenny Valladeau-Guilemond, Nathalie Bendriss-Vermare, Laurence Chaperot, Caroline Aspord

2628 related Products with: Hepatitis B virus exploits C-type lectin receptors to hijack cDC1s, cDC2s and pDCs.

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#33197619   2020/11/13 To Up

A study of multinucleated giant cells in esophageal cancer.

To evaluate the occurrence, abundance, distribution, nature and clinical significance of multinucleated giant cell (MGC) in esophageal cancer.
Hui Wang, Junjie Zhou, Jun Li, Yiqun Geng, Pei Meng, Changchun Ma, Ziqi Zhu, Weifeng Zhang, Liangli Hong, Yan Quan, Jiacong Wei, Qiongyi Huang, You Zhou, Zuoqing Su, Xiaoqing Zhu, Chuangzhen Chen, Shaobin Chen, Jiang Gu

1623 related Products with: A study of multinucleated giant cells in esophageal cancer.



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#33168071   2020/11/09 To Up

Scleroderma-specific autoantibodies embedded in immune complexes mediate endothelial damage: an early event in the pathogenesis of systemic sclerosis.

Consistently with their diagnostic and prognostic value, autoantibodies specific for systemic sclerosis (SSc) embedded in immune complexes (ICs) elicited a pro-inflammatory and pro-fibrotic cascade in healthy skin fibroblasts, engaging Toll-like receptors (TLRs) via their nucleic acid components. The objective of this study was to investigate the pathogenicity of SSc-ICs in endothelial cells.
Elena Raschi, Daniela Privitera, Caterina Bodio, Paola Adele Lonati, Maria Orietta Borghi, Francesca Ingegnoli, Pier Luigi Meroni, Cecilia Beatrice Chighizola

1320 related Products with: Scleroderma-specific autoantibodies embedded in immune complexes mediate endothelial damage: an early event in the pathogenesis of systemic sclerosis.

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