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#33673010   2021/02/14 To Up

Genome-Wide Identification and Characterization of Wheat 14-3-3 Genes Unravels the Role of TaGRF6-A in Salt Stress Tolerance by Binding MYB Transcription Factor.

14-3-3 proteins are a large multigenic family of general regulatory factors (GRF) ubiquitously found in eukaryotes and play vital roles in the regulation of plant growth, development, and response to stress stimuli. However, so far, no comprehensive investigation has been performed in the hexaploid wheat. In the present study, A total of 17 potential 14-3-3 gene family members were identified from the Chinese Spring whole-genome sequencing database. The phylogenetic comparison with six 14-3-3 families revealed that the majority of wheat genes might have evolved as an independent branch and grouped into ε and non-ε group using the phylogenetic comparison. Analysis of gene structure and motif indicated that 14-3-3 protein family members have relatively conserved exon/intron arrangement and motif composition. Physical mapping showed that wheat genes are mainly distributed on chromosomes 2, 3, 4, and 7. Moreover, most members in wheat exhibited significantly down-regulated expression in response to alkaline stress. VIGS assay and protein-protein interaction analysis further confirmed that TaGRF6-A positively regulated slat stress tolerance by interacting with a MYB transcription factor, TaMYB64. Taken together, our findings provide fundamental information on the involvement of the wheat 14-3-3 family in salt stress and further investigating their molecular mechanism.
Wenna Shao, Wang Chen, Xiaoguo Zhu, Xiaoyi Zhou, Yingying Jin, Chuang Zhan, Gensen Liu, Xi Liu, Dongfang Ma, Yongli Qiao

2747 related Products with: Genome-Wide Identification and Characterization of Wheat 14-3-3 Genes Unravels the Role of TaGRF6-A in Salt Stress Tolerance by Binding MYB Transcription Factor.

100.00 ug 25UG50 ug100.00 ug100.00 ug100.00 ug 100 UG5 x 50 ug100.00 ug0.1ml (1mg/ml)100 100 μg

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#33672342   2021/02/23 To Up

Chromatin Accessibility Is Associated with Artemisinin Biosynthesis Regulation in .

Glandular trichome (GT) is the dominant site for artemisinin production in . Several critical genes involved in artemisinin biosynthesis are specifically expressed in GT. However, the molecular mechanism of differential gene expression between GT and other tissue types remains elusive. Chromatin accessibility, defined as the degree to which nuclear molecules are able to interact with chromatin DNA, reflects gene expression capacity to a certain extent. Here, we investigated and compared the landscape of chromatin accessibility in leaf and GT using the Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) technique. We identified 5413 GT high accessible and 4045 GT low accessible regions, and these GT high accessible regions may contribute to GT-specific biological functions. Several GT-specific artemisinin biosynthetic genes, such as and , showed higher accessible regions in GT compared to that in leaf, implying that they might be regulated by chromatin accessibility. In addition, transcription factor binding motifs for MYB, bZIP, C2H2, and AP2 were overrepresented in the highly accessible chromatin regions associated with artemisinin biosynthetic genes in glandular trichomes. Finally, we proposed a working model illustrating the chromatin accessibility dynamics in regulating artemisinin biosynthetic gene expression. This work provided new insights into epigenetic regulation of gene expression in GT.
Limeng Zhou, Yingzhang Huang, Qi Wang, Dianjing Guo

1875 related Products with: Chromatin Accessibility Is Associated with Artemisinin Biosynthesis Regulation in .

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#33668977   2021/02/25 To Up

Comparative Transcriptome Analysis Reveals Key Genes and Pathways Involved in Prickle Development in Eggplant.

Eggplant is one of the most important vegetables worldwide. Prickles on the leaves, stems and fruit calyxes of eggplant may cause difficulties during cultivation, harvesting and transportation, and therefore is an undesirable agronomic trait. However, limited knowledge about molecular mechanisms of prickle morphogenesis has hindered the genetic improvement of eggplant. In this study, we performed the phenotypic characterization and transcriptome analysis on prickly and prickleless eggplant genotypes to understand prickle development at the morphological and molecular levels. Morphological analysis revealed that eggplant prickles were multicellular, lignified and layered organs. Comparative transcriptome analysis identified key pathways and hub genes involved in the cell cycle as well as flavonoid biosynthetic, photosynthetic, and hormone metabolic processes during prickle development. Interestingly, genes associated with flavonoid biosynthesis were up-regulated in developing prickles, and genes associated with photosynthesis were down-regulated in developing and matured prickles. It was also noteworthy that several development-related transcription factors such as bHLH, C2H2, MYB, TCP and WRKY were specifically down- or up-regulated in developing prickles. Furthermore, four genes were found to be differentially expressed within the locus interval. This study provides new insights into the regulatory molecular mechanisms underlying prickle morphogenesis in eggplant, and the genes identified might be exploited in breeding programs to develop prickleless eggplant cultivars.
Lei Zhang, Haoyun Sun, Tao Xu, Tianye Shi, Zongyun Li, Wenqian Hou

2175 related Products with: Comparative Transcriptome Analysis Reveals Key Genes and Pathways Involved in Prickle Development in Eggplant.

50 UG100 μg96 wells0.1ml (1mg/ml)300 units100 μg5mg 1 G100 μg100μg5ug

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#33668731   2021/02/25 To Up

Somatic Copy Number Alterations and Associated Genes in Clear-Cell Renal-Cell Carcinoma in Brazilian Patients.

Somatic copy number aberrations (CNAs) have been associated with clear-cell renal carcinoma (ccRCC) pathogenesis and are a potential source of new diagnostic, prognostic and therapeutic biomarkers. Recurrent CNAs include loss of chromosome arms 3p, 14q, 9p, and gains of 5q and 8q. Some of these regional CNAs are suspected of altering gene expression and could influence clinical outcomes. Despite many studies of CNAs in RCC, there are currently no descriptions of genomic copy number alterations in a Brazilian ccRCC cohort. This study was designed to evaluate the chromosomal profile of CNAs in Brazilian ccRCC tumors and explore clinical associations. A total of 92 ccRCC Brazilian patients that underwent nephrectomy at Barretos Cancer Hospital were analyzed for CNAs by array comparative genomic hybridization. Most patients in the cohort had early-stage localized disease. The most significant alterations were loss of 3p (87.3%), 14q (35.8%), 6q (29.3%), 9p (28.6%) and 10q (25.0%), and gains of 5q (59.7%), 7p (29.3%) and 16q (20.6%). Bioinformatics analysis revealed 19 genes mapping to CNA significant regions, including , , , , and . Moreover, gain of 5q34-q35.3 ( and ) and loss of 6q23.2-q23.3 () and 9p21.3 () had gene expression levels that correlated with TCGA data and was also associated with advanced disease features, such as larger tumors, Fuhrman 3, metastasis at diagnosis and death. The loss of region 14q22.1 which encompasses the gene was associated with poor overall survival. Overall, this study provides the first CNA landscape of Brazilian patients and pinpoints genomic regions and specific genes worthy of more detailed investigations. Our results highlight important genes that are associated with copy number changes involving large chromosomal regions that are potentially related to ccRCC tumorigenesis and disease biology for future clinical investigations.
Flávia Gonçalves Fernandes, Henrique Cesar Santejo Silveira, João Neif Antonio Júnior, Rosana Antunes da Silveira, Luis Eduardo Zucca, Flavio Mavignier Cárcano, André Octavio Nicolau Sanches, Luciano Neder, Cristovam Scapulatempo-Neto, Sergio Vicente Serrano, Eric Jonasch, Rui Manuel Reis, Adriane Feijó Evangelista

2067 related Products with: Somatic Copy Number Alterations and Associated Genes in Clear-Cell Renal-Cell Carcinoma in Brazilian Patients.



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#33668082   2021/02/24 To Up

Genome-Wide Identification and Analysis of the Transcription Factor Gene Family in Chili Pepper ( spp.).

The MYB transcription factor family is very large and functionally diverse in plants, however, only a few members of this family have been reported and characterized in chili pepper ( spp.). In the present study, we performed genome-wide analyses of the MYB family in , including phylogenetic relationships, conserved domain, gene structure organization, motif protein arrangement, chromosome distribution, chemical properties predictions, RNA-seq expression, and RT-qPCR expression assays. A total of 235 non-redundant MYB proteins were identified from , including R2R3-MYB, 3R-MYB, atypical MYB, and MYB-related subclasses. The sequence analysis of CaMYBs compared with other plant MYB proteins revealed gene conservation, but also potential specialized genes. Tissue-specific expression profiles showed that genes were differentially expressed, suggesting that they are functionally divergent. Furthermore, the integration of our data allowed us to propose strong candidates to be regulating phenylpropanoid, lignin, capsaicinoid, carotenoid, and vitamin C biosynthesis, providing new insights into the role of MYB transcription factors in secondary metabolism. This study adds valuable knowledge about the functions of genes in various processes in the genus.
Magda L Arce-Rodríguez, Octavio Martínez, Neftalí Ochoa-Alejo

1063 related Products with: Genome-Wide Identification and Analysis of the Transcription Factor Gene Family in Chili Pepper ( spp.).

25UG100 µl96100.00 ugProtein5 x 50 ug96T20 ul8 x 25 ul1 mg200ug

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#33657372   // To Up

MMB-FOXM1-driven premature mitosis is required for CHK1 inhibitor sensitivity.

To identify genes whose loss confers resistance to CHK1 inhibitors, we perform genome-wide CRISPR-Cas9 screens in non-small-cell lung cancer (NSCLC) cell lines treated with the CHK1 inhibitor prexasertib (CHK1i). Five of the top six hits of the screens, MYBL2 (B-MYB), LIN54, FOXM1, cyclin A2 (CCNA2), and CDC25B, are cell-cycle-regulated genes that contribute to entry into mitosis. Knockout of MMB-FOXM1 complex components LIN54 and FOXM1 reduce CHK1i-induced DNA replication stress markers and premature mitosis during Late S phase. Activation of a feedback loop between the MMB-FOXM1 complex and CDK1 is required for CHK1i-induced premature mitosis in Late S phase and subsequent replication catastrophe, indicating that dysregulation of the S to M transition is necessary for CHK1 inhibitor sensitivity. These findings provide mechanistic insights into small molecule inhibitors currently studied in clinical trials and provide rationale for combination therapies.
Timothy B Branigan, David Kozono, Amy E Schade, Peter Deraska, Hembly G Rivas, Larissa Sambel, Hunter D Reavis, Geoffrey I Shapiro, Alan D D'Andrea, James A DeCaprio

1000 related Products with: MMB-FOXM1-driven premature mitosis is required for CHK1 inhibitor sensitivity.

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#33656245   2021/03/03 To Up

Alternative splicing of MaMYB16L regulates starch degradation in banana fruit during ripening.

The alternative splicing of select genes is an important mechanism to regulate responses to endogenous and environmental signals in plants. However, the role of alternative splicing in regulating fruit ripening remains unclear. Here, we discovered that MaMYB16L, an R1-type MYB transcription factor, undergoes alternative splicing and generates two transcripts, the full-length isoform MaMYB16L and a truncated form MaMYB16S, in banana fruit. During banana fruit ripening, the alternative splicing process intensifies with downregulated MaMYB16L and upregulated MaMYB16S. Moreover, MaMYB16L is a transcriptional repressor that directly binds with the promoters of many genes associated with starch degradation and MaDREB2, a positive ripening regulator, and represses their expression. In contrast, MaMBY16S lacks a DNA-binding domain but competitively combines and forms nonfunctional heterodimers with functional MaMYB16L. MaMYB16L-MaMYB16S heterodimers decrease the binding capacity and transrepression activity of MaMYB16L. The downregulation of MaMYB16L and the upregulation of MaMYB16S, i.e., a decreased ratio of active to nonactive isoforms, facilitates the activation of ripening-related genes and thereby promotes fruit ripening. Furthermore, the transient overexpression of MaMYB16S promotes banana fruit ripening, whereas the overexpression of MaMYB16L delays this process. Therefore, the alternative splicing of MaMYB16L might generate a self-controlled regulatory loop to regulate banana fruit ripening. This article is protected by copyright. All rights reserved.
Guoxiang Jiang, Dandan Zhang, Zhiwei Li, Hanzhi Liang, Rufang Deng, Xingguo Su, Yueming Jiang, Xuewu Duan

2721 related Products with: Alternative splicing of MaMYB16L regulates starch degradation in banana fruit during ripening.

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#33651378   2021/03/02 To Up

Myb10-D confers PHS-3D resistance to pre-harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat.

Pre-harvest sprouting (PHS), the germination of grain before harvest, is a serious problem resulting in wheat yield and quality losses. Here, we mapped the PHS resistance gene PHS-3D from synthetic hexaploid wheat to a 2.4 Mb presence-absence variation (PAV) region and found that its resistance effect was attributed to the pleiotropic Myb10-D by integrated omics and functional analyses. Three haplotypes were detected in this PAV region among 262 worldwide wheat lines and 16 Aegilops tauschii, and the germination percentages of wheat lines containing Myb10-D was approximately 40% lower than that of the other lines. Transcriptome and metabolome profiling indicated that Myb10-D affected the transcription of genes in both the flavonoid and ABA biosynthesis pathways, which resulted in increases in flavonoids and ABA in transgenic wheat lines. Myb10-D activates NCED by biding the secondary wall MYB-responsive element (SMRE) to promote ABA biosynthesis in early wheat seed development stages. We revealed that the newly discovered function of Myb10-D confers PHS resistance by enhancing ABA biosynthesis to delay germination in wheat. The PAV harboring Myb10-D associated with grain color and PHS will be useful for understanding and selecting white grained PHS resistant wheat cultivars.
Jing Lang, Yuxin Fu, Yong Zhou, Mengping Cheng, Min Deng, Maolian Li, Tingting Zhu, Jian Yang, Xiaojiang Guo, Lixuan Gui, Linchuan Li, Zhongxu Chen, Yingjin Yi, Lianquan Zhang, Ming Hao, Lin Huang, Chao Tan, Guoyue Chen, Qiantao Jiang, Pengfei Qi, Zhien Pu, Jian Ma, Zehou Liu, Yujiao Liu, Ming-Cheng Luo, Yuming Wei, Youliang Zheng, Yongrui Wu, Dengcai Liu, JiRui Wang

2667 related Products with: Myb10-D confers PHS-3D resistance to pre-harvest sprouting by regulating NCED in ABA biosynthesis pathway of wheat.

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#33649234   // To Up

Posttranslational regulation of multiple clock-related transcription factors triggers cold-inducible gene expression in .

Cold stress is an adverse environmental condition that affects plant growth, development, and crop productivity. Under cold stress conditions, the expression of numerous genes that function in the stress response and tolerance is induced in various plant species, and the dehydration-responsive element (DRE) binding protein 1/C-repeat binding factor (DREB1/CBF) transcription factors function as master switches for cold-inducible gene expression. Cold stress strongly induces these genes. Therefore, it is important to elucidate the mechanisms of expression in response to cold stress to clarify the perception and response of cold stress in plants. Previous studies indicated that the central oscillator components of the circadian clock, CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY), are involved in cold-inducible expression, but the underlying mechanisms are not clear. We revealed that the clock-related MYB proteins REVEILLE4/LHY-CCA1-Like1 (RVE4/LCL1) and RVE8/LCL5 are quickly and reversibly transferred from the cytoplasm to the nucleus under cold stress conditions and function as direct transcriptional activators of expression. We found that CCA1 and LHY suppressed the expression of under unstressed conditions and were rapidly degraded specifically in response to cold stress, which suggests that they act as transcriptional repressors and indirectly regulate the cold-inducible expression of We concluded that posttranslational regulation of multiple clock-related transcription factors triggers cold-inducible gene expression. Our findings clarify the complex relationship between the plant circadian clock and the regulatory mechanisms of cold-inducible gene expression.
Satoshi Kidokoro, Kentaro Hayashi, Hiroki Haraguchi, Tomona Ishikawa, Fumiyuki Soma, Izumi Konoura, Satomi Toda, Junya Mizoi, Takamasa Suzuki, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki

2528 related Products with: Posttranslational regulation of multiple clock-related transcription factors triggers cold-inducible gene expression in .

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