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#32156055   2020/03/07 To Up

Opposing Effects of Adenosine and Inosine in Human Subcutaneous Fibroblasts May Be Regulated by Third Party ADA Cell Providers.

Human subcutaneous fibroblasts (HSCF) challenged with inflammatory mediators release huge amounts of ATP, which rapidly generates adenosine. Given the nucleoside's putative relevance in wound healing, dermal fibrosis, and myofascial pain, we investigated the role of its precursor, AMP, and of its metabolite, inosine, in HSCF cells growth and collagen production. AMP (30 µM) was rapidly (t½ 3 ± 1 min) dephosphorylated into adenosine by CD73/ecto-5'-nucleotidase. Adenosine accumulation (t½ 158 ± 17 min) in the extracellular fluid reflected very low cellular adenosine deaminase (ADA) activity. HSCF stained positively against A and A receptors but were A and A negative. AMP and the A receptor agonist, CGS21680C, increased collagen production without affecting cells growth. The A receptor antagonist, SCH442416, prevented the effects of AMP and CGS21680C. Inosine and the A receptor agonist, 2Cl-IB-MECA, decreased HSCF growth and collagen production in a MRS1191-sensitive manner, implicating the A receptor in the anti-proliferative action of inosine. Incubation with ADA reproduced the inosine effect. In conclusion, adenosine originated from extracellular ATP hydrolysis favors normal collagen production by HSCF via A receptors. Inhibition of unpredicted inosine formation by third party ADA cell providers (e.g., inflammatory cells) may be a novel therapeutic target to prevent inappropriate dermal remodeling via A receptors activation.
Carina Herman-de-Sousa, Ana Rita Pinheiro, Diogo Paramos-de-Carvalho, Maria Adelina Costa, Fátima Ferreirinha, Teresa Magalhães-Cardoso, Severino Ribeiro, Julie Pelletier, Jean Sévigny, Paulo Correia-de-Sá

1534 related Products with: Opposing Effects of Adenosine and Inosine in Human Subcutaneous Fibroblasts May Be Regulated by Third Party ADA Cell Providers.

100 μg1.00 flask1000 500 96 tests5ug25 TESTS1.00 flask100 μg1 mg96 tests

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#31586862   2019/09/19 To Up

A novel hierarchical stiff carbon foam with graphene-like nanosheet surface as the desired adsorbent for malachite green removal from wastewater.

A novel hierarchical stiff carbon foam (HSCF) was successfully prepared via a carbothermal reduction between the carbon foam with two-level pore structure and the AlO from aluminum sulfate, and used as a bulk adsorbent for removing malachite green (MG) dye. The structures of the HSCF were characterized using SEM, XRD, FTIR, BET, and XPS, and the effects of adsorption condition on the MG removal were studied through batch adsorption experiments. Results show that large-sized and complex-shaped HSCF can be easily fabricated with a high compression strength of 1.58 MPa at a low bulk density (0.10 g cm). The HSCF possesses a fluffy graphene-like nanosheet surface with a mesoporous structure and meanwhile exhibits good hydrophilicity loaded with aluminum hydroxide. The experimental maximum adsorption capacity for MG reaches 425.2 mg g with a relatively high partition coefficient of 9.38 mg g μM at the optimal condition. The experimental data are in good agreement with Langmuir isotherm and pseudo-second-order kinetic model, and meanwhile, the adsorption of MG onto the HSCF is a spontaneous and endothermic process. Also, the HSCF still exhibits good adsorption ability and stability after seven regeneration cycles.
Qiyun Zhang, Qilang Lin, Xialan Zhang, Yangfa Chen

1712 related Products with: A novel hierarchical stiff carbon foam with graphene-like nanosheet surface as the desired adsorbent for malachite green removal from wastewater.

250096 Tests1 kit100 tests1 kit10 Plates25 Tests1 kit1 kit

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#31183128   2019/04/10 To Up

Experimental study on seismic performance of a low-energy consumption composite wall structure of a pre-fabricated lightweight steel frame.

This study developed a low-energy consumption composite wall structure constructed with a pre-fabricated lightweight steel frame that is suitable for houses in villages and towns and evaluated its anti-seismic performance. A low-reversed cyclic-loading test was conducted on four full-scale pre-fabricated structure specimens, including a lightweight, concrete-filled steel tube (CFST) column frame specimen (abbreviated as SFCF), a lightweight CFST column frame composite wall specimen (abbreviated as SFCFW), an H-steel column frame specimen (abbreviated as HSCF) and an H-steel column frame composite wall specimen (abbreviated as HSCFW). The failure characteristics, hysteretic behaviour, strength, rigidity, ductility and energy dissipation capacity of each specimen were compared and analysed. The results demonstrated that the pre-fabricated, double L-shaped beam-column joint with a stiffener rib which was proposed in this study worked reliably and exhibited good anti-seismic performance. The yield, ultimate and frame yield loads of the specimen SFCFW were 1.72, 1.80 and 2.03 times higher than those of specimen SFCF. The yield load, ultimate load and frame yield loads of specimen HSCFW were 1.27, 1.68 and 1.82 times higher than those of specimen HSCF. This indicates that the embedded composite wall contributed significantly to the horizontal bearing capacities of the SFCF and HSCF specimens. The embedded composite wall was divided into multiple strip-shaped composite panels during failure and achieved a stable support for the frame in the later stages of elastoplastic deformation. The horizontal strips of the tongue-and-groove connection between the strip-shaped composite panels produced reciprocating bite displacements, and ultimately improved the structure's energy dissipation capacity significantly.
Jia Suizi, Cao Wanlin, Liu Zibin

1395 related Products with: Experimental study on seismic performance of a low-energy consumption composite wall structure of a pre-fabricated lightweight steel frame.

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#30447017   2018/11/16 To Up

Study on immortal conditions of chicken embryonic stem cells.

In recent years, considerable attention has been paid to chicken embryonic stem cells (ESCs) studies in relation to extensive applications in gene therapy and regenerative medicine. However, the approaches used are still immature. In this study, we showed that the chicken ESCs clones with a clear border can express alkaline phosphatase and marker proteins such as SSEA-1, SOX2, and OCT4 stably. In addition, culture medium containing 10 μmol/L of vitamin C (VC) could significantly promote the proliferation of ESCs cells. Moreover, ESCs transfected with p:enhanced green fluorescent protein (pEGFP)-hTERT could be subcultured more than tenth generations in culture medium containing exogenous factors (mLIF + bFGF + hSCF) and VC, and these ESCs clone could still be regenerated following cryopreservation. Quantitative real-time polymerase chain reaction results showed that there was no significant difference between SSEA-1, SOX2, and OCT4 expression during ESCs immortalization and that the tenth generation of ESCs was still able to express marker proteins SSEA-1, SOX2, and OCT4. Our results showed that an immobilized system for ESCs was established, and the ESCs were cultured in vitro maintaining their pluripotency.
Changhua Sun, Yingjie Wang, Kai Jin, Jiuzhou Song, Qisheng Zuo, Yani Zhang, Guohong Chen, Bichun Li

1789 related Products with: Study on immortal conditions of chicken embryonic stem cells.

1 x 10^6 cells/vial15ml1 mg100ml5 x 10A5 cells/vial10 ug130ml96 wells (1 kit)100ug Lyophilized100 μg500

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#27758159   2016/10/27 To Up

Comparison of Human Hematopoietic Reconstitution in Different Strains of Immunodeficient Mice.

Immunodeficient mice play a critical role in hematology research as in vivo models of hematopoiesis and immunology. Multiple strains have been developed, but hematopoietic stem cell engraftment and immune reconstitution have not been methodically compared among them. Four mouse strains were transplanted with human fetal bone marrow or adult peripheral blood CD34 cells: NSG, NSG-3GS, hSCF-Tg-NSG, and hSIRPα-DKO. Hematopoietic engraftment in the bone marrow, blood, spleen, and liver was evaluated by flow cytometry 12 weeks after transplant. The highest levels of human engraftment were observed in the liver, spleen, and bone marrow, whereas peripheral blood cell chimerism was notably less. The highest levels of tissue engraftment were in hSCF-Tg-NSG mice, but NSG mice exhibited the highest blood leukocyte engraftment. hSCF-Tg-NSG mice also exhibited the highest levels of CD133CD34 stem cells. hSIRPα-DKO engrafted poorly and exhibited poor breeding. Myelopoiesis was greatest in NSG-3GS mice, followed by hSCF-Tg-NSG and NSG mice, whereas B cell engraftment exhibited the opposite pattern. Engraftment of CD3 T cells, CD3CD161 T cells, and CD3CD56 NK cells was greatest in NSG-3GS mice. Mast cell engraftment was highest in hSCF-Tg-NSG mice, but was also elevated in spleen and livers of NSG-3GS mice. Basophils were most abundant in NSG-3GS mice. Overall, hSCF-Tg-NSG mice are the best recipient mice for studies requiring high levels of human hematopoiesis, stem cell engraftment, and an intermediate level of myelopoiesis, whereas NSG and NSG-3GS mice offer select advantages in the engraftment of certain blood cell lineages.
Ashley I Beyer, Marcus O Muench

2010 related Products with: Comparison of Human Hematopoietic Reconstitution in Different Strains of Immunodeficient Mice.

10 ug1 mg100 μg100 μg100 μg100 100 μg100μg100 μg96 wells (1 kit)0.1 mg100ug Lyophilized

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#26724416   2015/12/25 To Up

Improving the soluble expression and purification of recombinant human stem cell factor (SCF) in endotoxin-free Escherichia coli by disulfide shuffling with persulfide.

We here present a new method for the expression and purification of recombinant human stem cell factor (rhSCF(164)) in endotoxin-free ClearColi(®) BL21(DE3) cells harboring codon-optimized Profinity eXact™-tagged hSCF cDNA. Previously, we demonstrated that co-expression with thioredoxin increased the solubility of rhSCF in Escherichia coli BL21(DE3), and addition of l-arginine enhanced chromatography performance by removing the endotoxin-masked surface of rhSCF. Initially, we tried to express rhSCF in an endotoxin-free strain using a thioredoxin co-expression system, which resulted in significantly lower expression, possibly due to the stress imposed by overexpressed thioredoxin or antibiotics susceptibility. Therefore, we developed a new expression system without thioredoxin. External redox coupling was tested using persulfides such as glutathione persulfide or cysteine persulfide for the in vivo-folding of hSCF in the cytoplasm. Persulfides improved the protein solubility by accelerating disulfide-exchange reactions for incorrectdisulfides during folding in E. coli. Furthermore, the persulfides enhanced the expression level, likely due to upregulation of the enzymatic activity of T7 RNA polymerase. The recombinant protein was purified via affinity chromatography followed by cleavage with sodium fluoride, resulting in complete proteolytic removal of the N-terminal tag. The endotoxin-free fusion protein from ClearColi(®) BL21(DE3) could bind to the resin in the standard protocol using sodium phosphate (pH 7.2). Furthermore, purified rhSCF enhanced the proliferation and maturation of the human mast cell line LAD2. Thus, we conclude that use of the protein expression system employing E. coli by disulfide shuffling with persulfide addition could be a very useful method for efficient protein production.
Takafumi Ueda, Teruo Akuta, Takane Kikuchi-Ueda, Keitaro Imaizumi, Yasuo Ono

2823 related Products with: Improving the soluble expression and purification of recombinant human stem cell factor (SCF) in endotoxin-free Escherichia coli by disulfide shuffling with persulfide.

1 mg10 ug50 ug10 ug100 96 wells (1 kit)10 5 x 50 ug10 1mg2x 100ug5

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#25107671   2014/08/08 To Up

Cardiac-specific overexpression of human stem cell factor promotes epicardial activation and arteriogenesis after myocardial infarction.

The adult epicardium is a potential source of cardiac progenitors after myocardial infarction (MI). We tested the hypothesis that cardiomyocyte-specific overexpression of membrane-associated human stem cell factor (hSCF) enhances epicardial activation, epicardium-derived cells (EPDCs) production, and myocardial arteriogenesis post MI.
Fu-Li Xiang, Yin Liu, Xiangru Lu, Douglas L Jones, Qingping Feng

2500 related Products with: Cardiac-specific overexpression of human stem cell factor promotes epicardial activation and arteriogenesis after myocardial infarction.

2ug1 mg96 wells (1 kit)10 ug 100ul1100ug Lyophilized5 ug2ug62ug5 x 50 ug

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