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#34817370   2021/01/31 To Up

Chemical profiling, HPLC characterization and in-vitro antioxidant potential of Pakistani propolis collected from peripheral region of Faisalabad.

Propolis is a highly adhesive and resinous product of honey bee (Apis mellifera L.) which is produced from the exudations of plants. Bee propolis being a source of bioactive compounds like polyphenols and flavonoids imparts numerous biological properties including, antioxidant, anti-inflammatory, antimicrobial and anticancer activities. Present study was designed to elucidate the composition and antioxidant status of locally available propolis using in-vitro conditions. Propolis collected from locally found apiaries and its hydroalcoholic extract of propolis was prepared using different concentrations of ethanol and methanol. The results regarding proximate composition of propolis showed a higher proportion of ether extract (85.59±0.87%) and lowest contents of crude fiber (0.31±0.08%). Among the mineral's sodium, potassium and calcium was found in a concentration of 11.33±0.91, 52.10±2.9 and 10.53±0.83.59±0.23mg/Kg respectively whilst zinc was noticed as 3.59±0.23mg/Kg. HPLC characterization indicates a highest concentration of Chlorogenic acid 31.80±2.56mg/Kg whereas gallic acid (0.21±0.01mg/Kg) was found in lowest concentration among the polyphenols. Ethanol extract represents more phenolic contents, DPPH activity and antioxidant status as 327.30±14.89mg/gGAE, 73.18±4.43% and 60.59±4.38% accordingly in comparison to methanol and water extract. Bee propolis found an effective source of natural antioxidants which retards the production of free radicals and reactive oxygen species thus help to cope oxidative stress.
Muhammad Shahbaz, Tahir Zahoor, Rizwan Arshad, Saima Rafiq, Tahira Batool Qaisrani, Atif Liaqat, Muhammad Sameem Javed, Zainab Akbar, Nighat Raza, Shamas Murtaza, Umar Farooq, Muhammad Imran, Ahmed El-Ghorab, Umar Bacha, Ishtiaque Ahmad, Zaffar Mehmood, Rizwana Muzaffar, Tanweer Aslam Gondal, Syed Abdul Majeed Shah, Abdul Sattar Shah, Muhammad Akhtar, Muhammad Inam Afzal, Muhammad Umer

1986 related Products with: Chemical profiling, HPLC characterization and in-vitro antioxidant potential of Pakistani propolis collected from peripheral region of Faisalabad.

5 G100 μg100 μg0.1mg2 mL100 μg100 μg100 μg1,000 tests100 μg100 μg

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#34560112   2021/09/21 To Up

Role of melatonin in attenuation of hemodynamic response to intubation and anesthetic requirements: a randomized, controlled, double-blind study.

Melatonin has been studied to have anxiolytic, sedative, and analgesic effects. However, there is limited data on the effect of melatonin in the attenuation of hemodynamic response to intubation. We aimed to study whether preanesthetic oral melatonin attenuates hemodynamic responses to intubation and anesthetic requirements.
Rakesh Kumar, Kamlesh Kumari, Sarita Janweja, Rakesh Kumar, Manoj Verma, Ankur Sharma, Bharat Paliwal, Rama Kishan

1596 related Products with: Role of melatonin in attenuation of hemodynamic response to intubation and anesthetic requirements: a randomized, controlled, double-blind study.

100 UG100 μg100 1 kit1 mg100 25

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#34378137   2021/08/10 To Up

Bioaccumulation and transfer of zinc in soil plant and animal system: a health risk assessment for the grazing animals.

Heavy metals pollution has thorough worldwide apprehensions due to the instantaneous growth of industries. Farming regions are irrigated mainly with wastewater which contains both municipal and industrial emancipations. Keeping in view the above scenario, a study was designed in which three sites irrigated with ground, canal, and municipal wastewater in the District Jhang were selected to determine the zinc accumulation and its transfer in the soil, plant, and animal food chain. Zinc concentration was ranged as 18.85-35.59mg/kg in the soil, 26.42-42.67 mg/kg in the forage, and 0.982-2.85mg/kg in the animal samples. Investigated zinc concentration in soil and forages was found to be within the recommended WHO/FAO limits, but blood samples exceed the standards of NRC (2007). The maximum level of pollution load index (0.427-0.805mg/kg) and enrichment factor (0.373-0.894 mg/kg) for zinc was noticed upon wastewater irrigation. Daily intake (0.039 to 0.082 mg/kg/day) and health risk index (0.130 to 0.275 mg/kg/day) of zinc metal was higher in the buffaloes that feed on wastewater-irrigated forages. Bio-concentration factor (0.840 to 2.01mg/kg) for soil-forage was >1 which represents that these plants accumulated the zinc concentration into their tissues and raised health issues in grazing animals on consumption of wastewater-contaminated forages. As animal-derived products are part of human food, then zinc toxicity prevailed in livestock tissues ultimately affects the human food chain. Overall, findings of this study concluded that animal herds should be monitored periodically to devise preventive measures regarding the toxic level of heavy metals availability to livestock.
Fu Chen, Fatima Ghulam Muhammad, Zafar Iqbal Khan, Kafeel Ahmad, Ifra Saleem Malik, Asma Ashfaq, Majida Naeem, Muhammad Nadeem, Jing Ma, Muhammad Umer Farooq Awan, Shahzadi Mahpara, Shahid Mehmood

2575 related Products with: Bioaccumulation and transfer of zinc in soil plant and animal system: a health risk assessment for the grazing animals.

900 tests10 mg96tests5mg25 mg96 tests1000 tests100ul

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#33846926   2021/04/13 To Up

Long-term exposure to p-Nitrophenol induces hepatotoxicity via accelerating apoptosis and glycogen accumulation in male Japanese quails.

p-Nitrophenol (PNP) is the main end product of organophosphorus insecticides and a derivative of diesel exhaust particles. In addition to its unfavorable impact on reproductive functions in both genders, it also has various harmful physiological effects including lung cancer and allergic rhinitis. The identification of the cellular readout that functions in metabolic pathway perpetuation is still far from clear. This research aimed to study the impact of chronic PNP exposure on the health condition of the liver in Japanese quails. Quails were exposed to different concentrations of PNP as follows: 0.0 (control), 0.01mg (PNP/0.01), 0.1mg (PNP/0.1), and 1mg (PNP/1) per kg of body weight for 2.5 months through oral administration. Liver and plasma samples were collected at 1.5, 2, and 2.5 months post-treatment for biochemical, histopathology, and immunohistochemistry assessment. The plasma aspartate aminotransferase (AST) level was assessed enzymatically. The livers were collected for histopathology, glycogen accumulation, proliferating cell nuclear antigen (PCNA), and apoptosis assessment. Our results revealed an irregularity in body weight due to the long-term exposure of PNP with a significant reduction in liver weight. PNP treatment caused histopathological alterations in the hepatic tissues which increased in severity by the long-term exposure. The low dose led to mild degeneration with lymphocytic infiltration, while the moderate dose has a congestion effect with some necrosis; meanwhile severe hepatocyte degeneration and RBCs hemolysis were noticed due to high dose of PNP. Glycogen accumulation increased in hepatocytes by prolonged exposure to p-Nitrophenol with the highest intensity in the group treated by the high dose. Moderate and high doses of PNP resulted in a significant increase in apoptosis and hepatocytes' proliferation at the different time points after treatment. This increase is markedly notable and maximized at 2.5 months post-treatment. The damage occurred in a time-dependent manner. These changes reflected on the plasma hepatic enzyme AST that was clearly increased at 2.5 months of exposure. Therefore, it could be concluded that PNP has profound toxic effects on the liver in cellular level. Taking into consideration the time and dose factors, both have a synergistic effect on the accumulation of glycogen, apoptosis, and cellular proliferation, highlighting the power of cellular investigation which will potentially open the door for earlier medical intervention to counteract this toxicity. Collectively, PNP could have critical hurtful effects on the health of human beings, wild animals as well as livestock.
Eman A Ahmed, Howayda E Khaled, Ahmed K Elsayed

1293 related Products with: Long-term exposure to p-Nitrophenol induces hepatotoxicity via accelerating apoptosis and glycogen accumulation in male Japanese quails.

100 ug100 ug100 ug2 Pieces/Box100 μg100 μg 0.1 mg 0.1 mg100 μg1 mg96 wells (1 kit)100 μg

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#33516011   2020/12/10 To Up

Demand-oriented riboflavin supply of organic broiler using a feed material from fermentation of Ashbya gossypii.

Alternatives to riboflavin (vitamin B) production by recombinant microorganisms are needed in organic poultry production, but are cost-intensive, so that a demand-oriented riboflavin supply is necessary. Details on the riboflavin requirements of organic poultry are not available. A feed material with high native riboflavin content from fermentation of the filamentous fungus Ashbya gossypii was studied. Two runs with 800 Ranger Gold™ broilers each (40 pens with 20 animals) were conducted. The fattening period was divided into starter (S), grower (G) and finisher (F) stage. In the first run, a basal diet without riboflavin supplementation (NATIVE; 3.27, 3.50 and 3.16 mg riboflavin/kg DM in S, G and F) was compared to diets with supplementation at low (LOW; 5.30, 4.85 and 5.19 mg/kg in S, G and F), medium (MEDIUM; 7.56, 6.88 and 7.56 mg/kg in S, G and F) and high (HIGH; 10.38, 9.14 and 9.93 mg/kg in S, G and F) dosage. In the second run, different combinations of low and medium riboflavin supplementation were used in S, G and F diets: S-LOW (4.50 mg riboflavin/kg DM), G-MEDIUM (6.66 mg/kg), F-MEDIUM (5.71 mg/kg) (Treatment A), S-LOW (4.50 mg riboflavin/kg DM); G-LOW (4.92 mg/kg), F-LOW (4.01 mg/kg) (Treatment B); S-MEDIUM (6.37 mg/kg), G-MEDIUM (7.37 mg/kg), F-MEDIUM (5.07 mg/kg) (Treatment C); S-MEDIUM (6.37 mg/kg), G-LOW (5.28 mg/kg), F-LOW (4.22 mg/kg) (Treatment D). Body weight, feed and water consumption were recorded weekly, health and welfare indicators were scored bi-weekly. Slaughter traits were assessed for five males and females per pen. In the first run, NATIVE animals showed symptoms of riboflavin deficiency and lower live weights in the second week of age. Riboflavin contents of this group were increased to avoid further deficiency and recovery was observed. Feed conversion was better in HIGH (2.07) compared with NATIVE and LOW (2.11). At slaughter, treatments differed neither for foot pad dermatitis nor plumage cleanliness. In the second run, daily weight gains did not differ between treatments in any of the weeks. Feed conversion ranged between 1.99 and 2.04. Riboflavin deficiency was not observed in the second run, while treatment D showed superior economic efficiency. In conclusion, native contents of feed components (3.27 mg/kg DM) were not sufficient to meet the riboflavin demand and a total content of 4.50 mg/kg DM was identified as safe lower threshold. The levels rather according to commercial recommendations were not additionally beneficial to performance and health.
C Lambertz, J Leopold, S Ammer, F Leiber, B Thesing, C Wild, K Damme

2518 related Products with: Demand-oriented riboflavin supply of organic broiler using a feed material from fermentation of Ashbya gossypii.

2 Membrane supply5ml2 Membrane supply8 Array supply x 22 Membrane supply2 Membrane supply2 Membrane supply 5 G2 Membrane supply5ml2 Membrane supply8 Array supply x 2

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#33367127   2020/12/15 To Up

Seed bio-priming with tri-species consortia of phosphate solubilizing rhizobacteria (PSR) and its effect on plant growth promotion.

Three potential rhizobacteria namely (MTCC 10216), sp. (MTCC 9002) and (MTCC 8528) procured from IMTECH, Chandigarh (India) were evaluated individually and as consortia for its phosphate (P) solubilizing ability and effect of growth of fenugreek ( L.) and tomato ( L.). Phosphate solubilizing ability of these strains individually and as consortia was tested on Pikovskayas agar medium, Phosphate solubilizing agar medium and National Botanical Research Institute phosphate agar medium containing six different sources of insoluble inorganic phosphate such as tri-calcium phosphate (TCP), di-calcium phosphate (DCP), zinc phosphate (ZP), ferric phosphate (FP), sodium di-hydrogen phosphate (SP), and aluminum phosphate (AP), and two organic P such as calcium and sodium phytate. The maximum P solubilizing ability was recorded in consortium-4 having all three potential bacterial strains. Phosphate solubilization after 7 day of incubation was 37.9 mg/100 ml of TCP, 40.01 mg/100 ml of DCP, 15.79 mg/100 ml of FP, 43.02 mg/100 ml of SP, no solubilization of ZP and AP, 39.75 mg/100 ml of calcium phytate and 24.01mg/100 ml of sodium phytate. Seed germination and the other plant parameters such as plant height and weight significantly increased in fenugreek and tomato seeds, bio-primed with consortium-4 followed by consortium-3. After bio-priming of seeds in pot assay, the level of phosphorus in soil got increased by 54% in consortium-4 treated soil followed by consortium-3 (47%) over untreated control soil. Based on these findings, consoritium-4 could be recommended as a good bio-inoculant for fenugreek, tomato and other crops in comparison to individual strains and other consortia.
Pankaj Kumar, Abhinav Aeron, Niru Shaw, Ajay Singh, V K Bajpai, Shailja Pant, Ramesh Chandra Dubey

2715 related Products with: Seed bio-priming with tri-species consortia of phosphate solubilizing rhizobacteria (PSR) and its effect on plant growth promotion.

96T 100ul96T96T96T50 ug 50 ug 1 LITRE50 ug 100ul96T50 ug

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#33068993   2020/10/05 To Up

Protonation stabilized high As/F mobility red mud for Pb/As polluted soil remediation.

The hazardous red mud (RM) with high As/F mobility and heavy metal contaminated soil have constituted severe environmental threats. This work demonstrates a "waste to eco-material" strategy through a reliable and low-cost protonation approach to eliminate the As/F leaching risk of RM, and then recycle it as heavy metal passivators for Pb/As polluted soil remediation. The As/F anions have been immobilized by the protonated Fe/Al (hydr)oxides within RM via the formation of stable As/F compounds during the protonation process, which satisfies the requirement by the World Health Organization (As leaching <0.01 mg/L; F leaching <0.8 mg/L). Moreover, in the oilseed rape pot experiments, by adding 30 g/kg stabilized RM into Pb/As polluted soils (100 ~ 300 ppm), benefited from its large adsorption capacity, approximately 40.9 ~ 49.7% Pb and 40.8 ~ 54.8% As concentrations in the plant are reduced without adverse effects. The whole process for RM treatment and soil remediation is cost-effective, straightforward and eco-friendly without secondary pollution or soil degradation. This research provides a green chemical strategy to address both RM recycling and heavy metal contaminated soil remediation problems, which shows high economic feasibility and ecological benefits.
Dazhong Yang, Wanwan Deng, Ao Tan, Zheting Chu, Wenfei Wei, Renji Zheng, Yangzi Shangguan, Atsushi Sasaki, Masatoshi Endo, Hong Chen

2543 related Products with: Protonation stabilized high As/F mobility red mud for Pb/As polluted soil remediation.

100Tests100 tests100 assays400Tests1,000 tests96 Tests50 assays

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#33019940   // To Up

Trigonella foenum-graecum Seeds Oil Attenuated Inflammation and Angiogenesis in vivo through Down-Regulation of TNF-α.

Inflammation is a vital reaction of the natural immune system that protects against encroaching agents. However, uncontrolled inflammation can lead to complications. Trigonella foenumgraecum is traditionally used as an anti-inflammatory herb.
Muhammad Asif, Hafiz M Yousaf, Mohammad Saleem, Malik Saadullah, Tahir A Chohan, Muhammad U Shamas, Hafiza S Yaseen, Mahrukh, Muhammad U Yousaf, Maria Yaseen

2619 related Products with: Trigonella foenum-graecum Seeds Oil Attenuated Inflammation and Angiogenesis in vivo through Down-Regulation of TNF-α.

48 samples16 Arrays/Slide4 Sample Kit96 tests32-50 Sample Kit4 Arrays/Slide8 Sample Kit50ug4 Membranes/Box16 Arrays/Slide8 Sample Kit

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#32995330   // To Up

Comparison between Golnar product and placebo in heavy menstrual bleeding: A double-blind randomized clinical trial.

Golnar product is a poly herbal formulation advised by Persian medicine to control heavy menstrual bleeding (HMB). This study was conducted to compare the efficacy of this product with placebo in patients with HMB.
Fatemeh Yousefi, Maryam Kashanian, Ismail Nazem, Soodabeh Bioos, Omid Sadeghpour, Jale Aliasl, Fataneh Hashem-Dabaghian

2322 related Products with: Comparison between Golnar product and placebo in heavy menstrual bleeding: A double-blind randomized clinical trial.

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#32979684   2020/09/04 To Up

Acaricidal activity of (E)-cinnamaldehyde and α-bisabolol on populations of Rhipicephalus microplus (Acari: Ixodidae) with different resistance profiles.

This study aimed to investigate the acaricidal activity of (E)-cinnamaldehyde and α-bisabolol on populations of Rhipicephalus microplus with different resistance profiles. The adult immersion test (AIT) was used to characterize the susceptibility of tick populations (50 field populations) to synthetic acaricides: deltamethrin, amitraz, and chlorfenvinphos. The larval packet test (LPT) was used to determine the LC values for (E)-cinnamaldehyde (populations 1-25) and α-bisabolol (populations 26-50) at the concentrations of 0.31, 0.62, 1.25, 2.0, 2.5, 5.0 and 10.0 mg/mL. The susceptible strain Porto Alegre (POA) was used as a reference for calculating the resistance ratio (RR). In the AIT, deltamethrin did not show efficacy >95 % for any of the populations, whereas amitraz and chlorfenvinphos have presented efficacy >95 % for three (6 %) and 15 (30 %) populations, respectively. In the LPT, the LC values of (E)-cinnamaldehyde and α-bisabolol varied from 0.23 to 2.36 mg/mL and 1.57-3.01 mg/mL, respectively. The RR for (E)-cinnamaldehyde showed 20 (80 %) populations with values <1.0 and no population with values>1.5. As for α-bisabolol, only two (8%) populations have presented RR <1.0, whereas three (12 %) populations showed incipient resistance to this sesquiterpene (RR between 1.5 and 2.0). The results indicate that all studied tick populations showed low susceptibility to at least one of the commercial acaricides tested. In addition, comparison between the LC values of (E)-cinnamaldehyde and α-bisabolol for the field populations and the susceptible strain POA suggests that there is no cross-resistance of (E)-cinnamaldehyde and α-bisabolol for the tick populations evaluated, and that the differences in the LC values are due to population variations.
Paula Marchesini, Tatiane Pinheiro Novato, Simone Jaqueline Cardoso, Márcia Cristina de Azevedo Prata, Rafael Moreira do Nascimento, Guilherme Klafke, Lívio M Costa-Júnior, Ralph Maturano, Welber Daniel Zaneti Lopes, Vânia Rita Elias Pinheiro Bittencourt, Caio Monteiro

1744 related Products with: Acaricidal activity of (E)-cinnamaldehyde and α-bisabolol on populations of Rhipicephalus microplus (Acari: Ixodidae) with different resistance profiles.

5 G2.5 mg100 assays100ul0.1 mg 125 ml 96 Tests 96 Tests 100 assays10 mg2 x 96 well plate

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