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#38647822   2022/05/26 To Up

Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.

α-Alkenes (terminal alkenes) are important fuel and platform chemicals that are mainly produced from petroleum. Microbial synthesis might provide a sustainable approach for α-alkenes. In this work, we engineered the methylotrophic yeast Pichia pastoris to produce long-chain (C15:1, C17:1 and C17:2) α-alkenes via a decarboxylation of fatty acids. Combinatorial engineering, including enzyme selection, expression optimization and peroxisomal compartmentalization, enabled the production of 1.6 mg/L α-alkenes from sole methanol. This study represents the first case of α-alkene biosynthesis from methanol and also provides a reference for the construction of methanol microbial cell factories of other high-value chemicals.
Peng Cai, Yunxia Li, Xiaoxin Zhai, Lun Yao, Xiaojun Ma, Lingyun Jia, Yongjin J Zhou

1754 related Products with: Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.

1 mg100 µg1 mL100ug100ug6 ml100ug Lyophilized500 1mg2 mg100.00 ug0.5 mg

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#38647589   2022/05/18 To Up

From formic acid to single-cell protein: genome-scale revealing the metabolic network of Paracoccus communis MA5.

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Sheng Tong, Lizhi Zhao, Daling Zhu, Wuxi Chen, Limei Chen, Demao Li

1335 related Products with: From formic acid to single-cell protein: genome-scale revealing the metabolic network of Paracoccus communis MA5.

10 500 gm.1 mg 25 ml 100 ug1.00 flask 100ul200 ug100ug Lyophilized

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#38647583   2022/08/29 To Up

Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display.

Bacterial cis-epoxysuccinic acid hydrolases (CESHs) are intracellular enzymes used in the industrial production of enantiomeric tartaric acids. The enzymes are mainly used as whole-cell catalysts because of the low stability of purified CESHs. However, the low cell permeability is the major drawback of the whole-cell catalyst. To overcome this problem, we developed whole-cell catalysts using various surface display systems for CESH[L] which produces L(+)-tartaric acid. Considering that the display efficiency depends on both the carrier and the passenger, we screened five different anchoring motifs in Escherichia coli. Display efficiencies are significantly different among these five systems and the InaPbN-CESH[L] system has the highest whole-cell enzymatic activity. Conditions for InaPbN-CESH[L] production were optimized and a maturation step was discovered which can increase the whole-cell activity several times. After optimization, the total activity of the InaPbN-CESH[L] surface display system is higher than the total lysate activity of an intracellular CESH[L] overexpression system, indicating a very high CESH[L] display level. Furthermore, the whole-cell InaPbN-CESH[L] biocatalyst exhibited good storage stability at 4 °C and considerable reusability. Thereby, an efficient whole-cell CESH[L] biocatalyst was developed in this study, which solves the cell permeability problem and provides a valuable system for industrial L(+)-tartaric acid production.
Rui Zhou, Sheng Dong, Yingang Feng, Qiu Cui, Jinsong Xuan

1381 related Products with: Development of highly efficient whole-cell catalysts of cis-epoxysuccinic acid hydrolase by surface display.

2 Pieces/Box2 Pieces/Box96T 5 G2 Pieces/Box1 ml2 Pieces/Box

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#38647109   2024/04/22 To Up

High-density generation of spatial transcriptomics with STAGE.

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Shang Li, Kuo Gai, Kangning Dong, Yiyang Zhang, Shihua Zhang

2657 related Products with: High-density generation of spatial transcriptomics with STAGE.

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#38647075   // To Up

Simple, sensitive, and visual detection of 12 respiratory pathogens with one-pot-RPA-CRISPR/Cas12a assay.

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Qi Tan, Yaoqiang Shi, Chenlu Duan, Qingyuan Li, Tao Gong, Shilin Li, Xiaoqiong Duan, He Xie, Yujia Li, Limin Chen

2545 related Products with: Simple, sensitive, and visual detection of 12 respiratory pathogens with one-pot-RPA-CRISPR/Cas12a assay.

100tests50 mg10 plates100tests100tests50 Test96 tests100tests100tests100tests

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#38647045   2024/04/22 To Up

Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a.

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Lorenzo Olivi, Cleo Bagchus, Victor Pool, Ezra Bekkering, Konstantin Speckner, Hidde Offerhaus, Wen Y Wu, Martin Depken, Koen J A Martens, Raymond H J Staals, Johannes Hohlbein

1491 related Products with: Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a.

2.5 mg0.1ml (1mg/ml)25 mg100ug100 TESTS 5 G100 stainings 5 G25 Tests100ug

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