Gentaur Pub

#38652923   2024/04/23 To Up

Prevalence of cranial autonomic symptoms in frequent episodic tension-type headache: A post hoc analysis of the cross-sectional Migraine in Poland study.

Cranial autonomic symptoms (CASs) include lacrimation, conjunctival injection, rhinorrhea, nasal congestion, facial flushing or sweating, ptosis, and myosis. These symptoms may be associated with trigeminal autonomic cephalalgias (TACs) and migraine.
Marcin Straburzyński, Marta Waliszewska-Prosół, Magdalena Nowaczewska, Ewa Katarzyna Czapinska-Ciepiela, Anna Gryglas-Dworak, Slawomir Budrewicz

2474 related Products with: Prevalence of cranial autonomic symptoms in frequent episodic tension-type headache: A post hoc analysis of the cross-sectional Migraine in Poland study.

11 mL100.00 ug50ul1 mL

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#38652868   2024/04/23 To Up

Lithium Metal-Compatible Antifluorite Electrolytes for Solid-State Batteries.

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Pengcheng Yu, Haochang Zhang, Fiaz Hussain, Jing Luo, Wen Tang, Jiuwei Lei, Lei Gao, Denys Butenko, Changhong Wang, Jinlong Zhu, Wen Yin, Hao Zhang, Songbai Han, Ruqiang Zou, Wei Chen, Yusheng Zhao, Wei Xia, Xueliang Sun

2402 related Products with: Lithium Metal-Compatible Antifluorite Electrolytes for Solid-State Batteries.

100 Tests / Kit1 mg5 mg1 pack5 mg5 mg10 ml100 mg25 mg 500 ml 50 mg25 mg

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#38652732   2024/04/23 To Up

Optogenetic manipulation of lysosomal physiology and autophagy-dependent clearance of amyloid beta.

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Wenping Zeng, Canjun Li, Ruikun Wu, Xingguo Yang, Qingyan Wang, Bingqian Lin, Yanan Wei, Hao Li, Ge Shan, Lili Qu, Chunlei Cang

1688 related Products with: Optogenetic manipulation of lysosomal physiology and autophagy-dependent clearance of amyloid beta.

100ug Lyophilized100ug100ug100ug Lyophilized96 tests100ug100ug100 100ug 5 G100ul100ug Lyophilized

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#38652546   2024/04/23 To Up

Threonine dehydrogenase regulates neutrophil homeostasis but not H3K4me3 levels in zebrafish.

l-threonine dehydrogenase (Tdh) is an enzyme that links threonine metabolism to epigenetic modifications and mitochondria biogenesis. In vitro studies show that it is critical for the regulation of trimethylation of histone H3 lysine 4 (H3K4me3) levels and cell fate determination of mouse embryonic stem cells (mESCs). However, whether Tdh regulates a developmental process in vivo and, if it does, whether it also primarily regulates H3K4me3 levels in this process as it does in mESCs, remains elusive. Here, we revealed that, in zebrafish hematopoiesis, tdh is preferentially expressed in neutrophils. Knockout of tdh causes a decrease in neutrophil number and slightly suppresses their acute injury-induced migration, but, unlike the mESCs, the level of H3K4me3 is not evidently reduced in neutrophils sorted from the kidney marrow of adult tdh-null zebrafish. These phenotypes are dependent on the enzymatic activity of Tdh. Importantly, a soluble supplement of nutrients that are able to fuel the acetyl-CoA pool, such as pyruvate, glucose and branched-chain amino acids, is sufficient to rescue the reduction in neutrophils caused by tdh deletion. In summary, our study presents evidence for the functional requirement of Tdh-mediated threonine metabolism in a developmental process in vivo. It also provides an animal model for investigating the nutritional regulation of myelopoiesis and immune response, as well as a useful tool for high-throughput drug/nutrition screening.
Ning-Zhe Li, Zi-Xuan Wang, Fan Zhang, Chang-Zhou Feng, Yi Chen, Dian-Jia Liu, Shu-Bei Chen, Yi Jin, Yuan-Liang Zhang, Yin-Yin Xie, Qiu-Hua Huang, Lan Wang, Bing Li, Xiao-Jian Sun

1015 related Products with: Threonine dehydrogenase regulates neutrophil homeostasis but not H3K4me3 levels in zebrafish.

100 μg100 μg100 assays100 μg100 μg25 mg100 μg 5 G100 μg 25 G100 μg100 μg

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#38652440   2024/04/23 To Up

CRISPR-Cas13a-Triggered DNAzyme Signal Amplification-Based Colorimetric miRNA Detection Method and Its Application in Evaluating the Anxiety.

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Na Yan, Zhiwei Hu, Lingling Zhang

2085 related Products with: CRISPR-Cas13a-Triggered DNAzyme Signal Amplification-Based Colorimetric miRNA Detection Method and Its Application in Evaluating the Anxiety.

100tests100tests100tests100tests100tests100tests100tests100tests100tests100tests100tests 100ul

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#38652405   2024/04/23 To Up

Efficient gene editing in the slow-growing, non-sporulating, melanized, endophytic fungus Berkleasmium sp. Dzf12 using a CRISPR/Cas9 system.

The endophytic fungus Berkleasmium sp. Dzf12 that was isolated from Dioscorea zingiberensis, is a proficient producer of palmarumycins, which are intriguing polyketides of the spirobisnaphthalene class. These compounds displayed a wide range of bioactivities, including antibacterial, antifungal, and cytotoxic activities. However, conventional genetic manipulation of Berkleasmium sp. Dzf12 is difficult and inefficient, partially due to the slow-growing, non-sporulating, and highly pigmented behavior of this fungus. Herein, we developed a CRISPR/Cas9 system suitable for gene editing in Berkleasmium sp. Dzf12. The protoplast preparation was optimized, and the expression of Cas9 in Berkleasmium sp. Dzf12 was validated. To assess the gene disruption efficiency, a putative 1, 3, 6, 8-tetrahydroxynaphthalene synthase encoding gene, bdpks, involved in 1,8-dihydroxynaphthalene (DHN)-melanin biosynthesis, was selected as the target for gene disruption. Various endogenous sgRNA promoters were tested, and different strategies to express sgRNA were compared, resulting in the construction of an optimal system using the U6 snRNA-1 promoter as the sgRNA promoter. Successful disruption of bdpks led to a complete abolishment of the production of spirobisnaphthalenes and melanin. This work establishes a useful gene targeting disruption system for exploration of gene functions in Berkleasmium sp. Dzf12, and also provides an example for developing an efficient CRISPR/Cas9 system to the fungi that are difficult to manipulate using conventional genetic tools.
Siji Zhao, Ruya Yin, Mengwei Zhang, Ziqi Zhai, Zhen Shen, Yan Mou, Dan Xu, Ligang Zhou, Daowan Lai

2638 related Products with: Efficient gene editing in the slow-growing, non-sporulating, melanized, endophytic fungus Berkleasmium sp. Dzf12 using a CRISPR/Cas9 system.

1300 units

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#38652321   2024/04/23 To Up

Advances in miniature CRISPR-Cas proteins and their applications in gene editing.

The CRISPR-Cas system consists of Cas proteins and single-stranded RNAs that recruit Cas proteins and specifically target the nucleic acid. Some Cas proteins can accurately cleave the target nucleic acid under the guidance of the single-stranded RNAs. Due to its exceptionally high specificity, the CRISPR-Cas system is now widely used in various fields such as gene editing, transcription regulation, and molecular diagnosis. However, the huge size of the most frequently utilized Cas proteins (Cas9, Cas12a, and Cas13, which contain 950-1,400 amino acids) can limit their applicability, especially in eukaryotic gene editing, where larger Cas proteins are difficult to deliver into the target cells. Recently discovered miniature CRISPR-Cas proteins, consisting of only 400 to 800 amino acids, offer the possibility of overcoming this limitation. This article systematically reviews the latest research progress of several miniature CRISPR-Cas proteins (Cas12f, Cas12j, Cas12k, and Cas12m) and their practical applications in the field of gene editing.
Huimin Wu, Yixiang Sun, Yimai Wang, Liqiang Luo, Yizhi Song

1265 related Products with: Advances in miniature CRISPR-Cas proteins and their applications in gene editing.

101 Set100 7 x 25 ul

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#38652294   2024/04/23 To Up

Plant diversity increases spatial stability of aboveground productivity in alpine grasslands.

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Zeng Cui, Jian Sun, Gao-Lin Wu

2195 related Products with: Plant diversity increases spatial stability of aboveground productivity in alpine grasslands.

4/120 Packing /sleeve/bo4/120 Packing /sleeve/bo96tests4/120 Packing /sleeve/bo1 Set1 Set5 mg 0.1 mg

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#38652293   2024/04/23 To Up

Intraspecific alternative phenotypes contribute to variation in species' strategies for growth.

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Samantha J Worthy, María N Umaña, Caicai Zhang, Luxiang Lin, Min Cao, Nathan G Swenson

2168 related Products with: Intraspecific alternative phenotypes contribute to variation in species' strategies for growth.

100 μg 100ul25 µg4 Membranes/Box100 ml50 ug 96T 100ul1 kit96T 100 G

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